Determination of hydroxylysylpyridinoline in tissues and urine by high-performance liquid chromatography.
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Amino Acids↗
Biomedical subjects
M Adam
Publications and source records attributed to M Adam.
Structural alterations of p(HEMA)--collagen implants.
Samples of linear (additionally crosslinked) p(HEMA) with different amounts of fibrillar collagen were implanted into the popliteal region of rats. After 3 month, the implanted materials were harvested and examined by SEM. The implants underwent marked structural or morphological changes. While the fibrillar collagen was readily resorbed by invading cells, the synthetic constituent persisted to biodegradation. The p(HEMA) residues were shaped into spherical particles, approx. 1-15 microns in diameter. The possible fate of these microparticles in the host organism is discussed.
Animals↗
The influence of some non-steroidal anti-inflammatory drugs on the retraction of collagen lattices.
The effects of some antirheumatics on the formation and retraction of collagen lattices seeded with fibroblasts have been studied. Among the antirheumatics, diclofenac was the most active inhibitor of lattice retraction, then tropesin and to a lesser extent indomethacin. Ibuprofen which is known as a very slight inhibitor of protein synthesis was able to significantly enhance lattice retraction when 10 micrograms/ml (48.5 microM) and 50 micrograms/ml (242 microM) were used.
Anti-Inflammatory Agents, Non-Steroidal↗
Use of the metal replica technique in the electron microscopy of collagen.
The authors present a survey of visualization of the individual types of collagen by the metal replica technique, special attention was paid to the visualization of type IX collagen, which, together with type XI, is a minor component of cartilage collagen. The molecule of this collagen type contains three collagen and four non-collagen domains and during the isolation involving pepsin digestion it is cleaved into MHW and LMW fragments. The HMW fragment can be further cleaved into shorter and longer arm. Results of statistical evaluation of 199 measured molecular fragments showed the following length: whole molecule 194 +/- 23 nm, HMW 136 +/- 16 nm, the longer arm of the HMW 87 +/- +/- 12 nm and the LMW of shorter arm of the HMW 43 +/- 9 nm.
Collagen↗
[Changes in collagen biosynthesis in patients with hip joint replacement surgery and reoperation].
The authors investigated the intensity of collagen synthesis in patients subjected to operation or re-operation of a total endoprosthesis of the hip joint. The patients suffered from osteoarthritis, rheumatoid arthritis and ankylosing spondylitis. The authors assessed the activity of collagen glucosyl transferase (S-GGT) and the concentration of the N-terminal propeptide procollagen type III by two methods (S-Pro III-N-P and S-Fab) in serum before and after operation. A significant rise of S-GGT and S-Fab, as compared with controls, occurred only after operation while S-Pro III-N-P was elevated already before operation. S-GGT did not differ before and after operation, while N-propeptide concentration rose when either method was used.
Adult↗
Study on the hematopoietic stem cells from umbilical cord blood.
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Blood Cell Count↗
Retroviral-mediated gene transfer of the leukocyte integrin CD18 subunit.
Children with leukocyte adherence deficiency (LAD) exhibit heterogeneous defects in the leukocyte integrin CD18 subunit that prevent surface expression of functional CD11/CD18 leukocyte integrin adherence complexes. We used a retroviral vector, designated LCD18SN, to transfer the CD18 cDNA into K562 human myeloid leukemia cells and into EBV B-cells from a child with LAD. Transfer of the LCD18SN retroviral construct, which expresses the CD18 cDNA from the Moloney Murine leukemia virus (MoMLV) long terminal repeat (LTR), into K562 cells resulted in relatively high levels of CD18 mRNA and intracellular protein. Retroviral-mediated gene transfer of CD18 into LAD EBV B-cells resulted in low, but readily measurable, levels of surface expression of the CD11a/CD18 complex in these previously deficient lymphocytes. The reconstitution of surface expression of the CD11a/CD18 complex by gene transfer of the CD18 cDNA into LAD EBV B-cells indicates that this syndrome represents a candidate disorder for gene therapy.
Antigens, CD↗
Chromogenic depsipeptide substrates for beta-lactamases and penicillin-sensitive DD-peptidases.
Various ester and thioester derivatives of hippuric acid have been prepared which were substrates of both beta-lactamases and DD-peptidases. The thioesters were more rapidly hydrolysed by nearly all the enzymes. Surprisingly, the enzymes acted rather efficiently on substrates which did not contain any chiral centre.
Amino Acid Sequence↗
HLA-DR and pre-eclampsia in Edinburgh.
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Alleles↗
The effect of liquid nitrogen submersion on cryopreserved human heart valves.
Cryopreservation and storage of human heart valves have become an accepted means of maintaining a usable supply of heart valves for outflow track reconstructive surgery. Valves are typically stored at the vapor phase temperature of liquid nitrogen, -130 degrees C and below, to reduce the chance of recrystallization within the tissues. Concern over the effects of submersion of the valves in liquid nitrogen, i.e., plunging to -196 degrees C, prompted this study. Cryopreserved valves were plunged into liquid nitrogen, held for 5 min, and then processed (thawed) by standardized protocols. The thawed valves were then assessed using scanning electron microscopy and the more traditional histology at the light microscope level. Cuspal tissues plunged into liquid nitrogen appear to have numerous microfractures over both surfaces of the tissue, penetrating into the collagen/proteoglycan matrix. Control cryopreserved valves do not exhibit these microfractures. Histologically, the submerged valves appear normal. The clinical use of valves which have been submerged in liquid nitrogen is discussed.
Cryopreservation↗
Preparation of factor-VIII-depleted plasma with antibodies and its use for the assay of factor VIII.
Standardized factor VIII (FVIII)-deficient plasma is necessary for the clotting assay of this protein. Ethical considerations and prevalence of HIV seropositivity in hemophilia A patients have required the replacement of severe hemophilia A plasma by another specific substrate for FVIII clotting assays. FVIII-deficient plasma was prepared by immunodepletion using polyclonal anti-von-Willebrand factor (vWF) and monoclonal anti-FVIII antibodies coated to agarose beads. This plasma, deficient in both FVIII and vWF, allows the evaluation of FVIII activity with a detection limit of 1% as compared to normal plasma. This immunodepleted plasma was compared to plasma from severe hemophilia A for the FVIII determination in plasma from normal subjects, severe and mild hemophiliacs, patients with von Willebrand disease and dicoumarol-treated patients. In all cases, similar values were obtained with both reagents at various levels of FVIII, with a correlation coefficient of 0.994. Such a good correlation was also obtained for the assay of FVIII in concentrates and in plasma from hemophiliacs following infusion. This immunodeficient plasma thus represents a suitable reagent for the FVIII clotting assay and provides an accurate alternative to the use of hemophilic plasma.
Antibodies↗
Collection, cryopreservation and autologous transplantation with circulating hemopoietic stem cells in patients with multiple myeloma and lymphoma.
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Blood Transfusion, Autologous↗
Hematopoietic stem cell potential from umbilical cord blood.
We studied the conditions of collection and isolation of hematopoietic cells from cord blood in order to optimise the sampling. A statistically significant correlation was found between the total stem cell content of the samples and the time of delivery suggesting that the quantity of hematopoietic stem cells available is higher when cord blood collection is performed earlier during pregnancy. Attempt to isolate the white cells resulted in a dramatic loss of stem cells. Factors affecting cell recovery and purification must be investigated in order to optimize cord blood cell banking.
Blood Specimen Collection↗
Comparison of the influence of gelatine and collagen substrates on growth of chondrocytes.
The effect of culture substrates on adhesion and growth of chondrocytes and the influence of varying amounts of foetal calf serum on cell proliferation are compared. Skin and cartilage gelatines and type II collagen were used as substrates. The cells used in the experiments were the primary cultures of chondrocytes, frozen primary cultures of chondrocytes and chondrocytes after the 1st subculture. Cartilage gelatine had a more marked influence on cell proliferation than skin gelatine, especially in primary cultures. The differences were off in cells after the 1st subculture. Differences in the number of cells are evident also after decreasing the serum concentration in culture medium.
Animals↗
Separation of collagens by capillary zone electrophoresis.
Collagen (types I, II, V, IX and XI) constituting polypeptide chains and their polymers and cyanogen bromide-cleaved peptides of collagen type I and type III were investigated by means of capillary zone electrophoresis. Separations were effected in 2.5 mM sodium tetraborate buffer in less than 15 min. A 50 cm x 0.1 mm I.D. fused-silica capillary was used. The separations were run at 18 kV per capillary. The results of the separation were monitored at 220 nm with an on-tube detection system. Using the Offord equation, relative retention times of cyanogen bromide cleavage fragments were plotted against M(2-3)/Z, where M is the molecular mass of a polypeptide and Z its valency. A linear relationship was observed. Collagen alpha-chains and their polymers were also satisfactorily resolved.
Collagen↗
[Collagen as a biomaterial].
The authors discuss the characteristics of different types of collagen, their incidence, structure, chemical composition and physical properties with regard to their use as biomaterial. They also mention the antigenicity, effect on platelet aggregation, cytodifferentiation and cellular proliferation. The authors discuss different biomedical applications of collagen and methods of its sterilization.
Biocompatible Materials↗
Markers of collagen metabolism in sera of patients with various rheumatic diseases.
The activity of galactosylhydroxylysyl glucosyltransferase (S-GGT) and concentration of the amino-terminal propeptide of type III procollagen, measured with two different radioimmunoassays, S-Pro(III)-N-P and S-Fab, were determined in the sera of 209 patients with various rheumatic diseases. The mean values for all these three assays were elevated in the patients with rheumatoid arthritis, whereas only the mean value for S-GGT was elevated in osteoarthrosis. The mean S-GGT but not S-Pro(III)-N-P or S-Fab was also elevated in psoriatic arthritis, ankylosing spondylitis and systemic lupus erythematosus. S-GGT correlated significantly both with S-Pro(III)-N-P and S-Fab in the pooled group of all the patients and in the cases of rheumatoid arthritis and psoriatic arthritis, and also with S-Fab in the cases of osteoarthrosis. S-Pro(III)-N-P and S-Fab correlated with each other in every disease group. The ratio S-Fab:S-Pro(III)-N-P was significantly higher in the patients with osteoarthrosis, ankylosing spondylitis and systemic lupus erythematosus than in those with rheumatoid arthritis. The data indicate that definite changes can be seen in the values of serum markers of collagen metabolism in rheumatoid arthritis, psoriatic arthritis, osteoarthrosis, ankylosing spondylitis and systemic lupus erythematosus, the most sensitive indicator being S-GGT.
Adult↗
Separation methods for the study of collagen and treatment of collagen disorders.
Liquid chromatographic and electrophoretic methods applicable to the separation of collagen and its fragments are reviewed. Special attention is paid to the separation of both stabile and labile crosslinking elements. Identification procedures exploiting the mapping of either collagen alpha-chains or of cyanogen bromide fragments are discussed. These methods can be used for diagnosing inborn errors of collagen metabolism using bioptic or necroptic samples. Analysis of urinary hydroxyproline-containing peptides or the determination of peptidically bound pyridinoline is suitable for measuring the intensity of collagen metabolism.
Amino Acids↗