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Biomedical subjects

M Adachi

Publications and source records attributed to M Adachi.

At least 649 records · Page 36Linked to original sources
PubMed1987

Fetal-type creatine kinase in rat fast and slow muscles during denervation and reinnervation.

Creatine kinase (CK) has three forms of isozymes; CK-BB, CK-MB, and CK-MM. In adult rats they show a specific tissue distribution: the BB form in the brain, the MB form in the heart, and the MM form in skeletal muscle. In embryonic skeletal muscles only the BB and MB forms are found. Adult slow-twitch muscles contain more fetal type creatine kinase (CK-B) than do fast-twitch muscles. In the present experiment the effect of denervation and reinnervation on the CK-B concentration was investigated in rat fast (extensor digitorum longus)- and slow (soleus)-twitch muscles by a highly sensitive immunoassay. Denervation of these muscles produced a progressive increase in CK-B concentration in both muscles. When the sciatic nerve was cut and immediately sutured, the CK-B concentration in both muscles showed a gradual reduction after an initial increase. By the 34th postoperative week the CK-B concentration in the soleus was about one-half that of the contralateral control, whereas that in the extensor digitorum longus was nearly normal. After cross union of the nerves innervating the muscles, the CK-B concentration in the soleus was reduced at 35 weeks to about one-half normal, but that in the extensor digitorum longus was always higher than the control value. After self-reunion of the nerves, the CK-B concentration at the 20th week was approximately normal in the extensor digitorum longus and significantly increased in the soleus. We suggest that the motoneurons normally innervating the extensor digitorum longus have a greater capability in suppressing the production of CK-B than do the soleus motoneurons.

Animals↗
PubMed1987

Carbohydrate moieties of peanut agglutinin receptors isolated from human gastric cancer cells.

Receptors for peanut agglutinin (PNA) were isolated from Kato III human gastric cancer cells by affinity chromatography on PNA agarose, and were labeled by the galactose oxidase-NaB3H4 method. Alkaline NaBH4 treatment of the labeled receptors released two small oligosaccharide alcohols, which were identified as Gal beta 1----3GalNAc-ol and Gal beta 1----4GlcNAc beta 1----6(Gal beta 1----3)GalNAc-ol. Higher oligosaccharides and glycopeptides of both N- and O-linked type were also detected, but they did not appear to bear PNA binding sites. The presence of oligo-N-acetyllactosamine units in the N-linked type sugars was indicated by endo-beta-galactosidase digestion.

Carbohydrates↗
PubMed1987

Comparative study of subjective fatigue between automatic transmission bus drivers and manual transmission bus drivers.

The difference in the physiological condition of drivers of manual transmission buses (MTB) and automatic transmission buses (ATB) was examined from the viewpoint of occupational health. This study was based on a self-administered questionnaire which involved items concerning subjective fatigue complaints. No differences in the mental fatigue and stress between MTB drivers and ATB drivers were observed. Although ATB drivers tended to feel less physical fatigue than MTB drivers, the difference was not statistically significant. From these results, it was suggested that there was little difference in the subjective fatigue between ATB drivers and MTB drivers.

Adult↗
PubMed1987

IgA binding factors and Fc receptors for IgA: comparative studies between IgA and IgE Fc receptor systems.

The expression of Fc receptors (FcR) for IgA (Fc alpha R) as well as for IgE (Fc epsilon R) on T lymphocytes (T cells) is enhanced or up regulated by the corresponding class of immunoglobulins (Ig). The production of class-specific regulatory factors binding to IgA and IgE (IgA binding factor [IgA-BF]; IgE binding factor [IgE-BF]) is also induced by these respective ligands. Murine IgA-BFs produced by a T hybridoma T2D4 and concanavalin A-activated spleen cells suppressed the in vitro IgA antibody responses of pokeweed mitogen-stimulated mouse spleen cells class-specifically. Human IgA antibody response was also suppressed by the murine IgA-BF. Similar suppressive IgA-BF is also produced by a human natural killer (NK)-like cell line (YT), which has no rearrangement of the T cell receptor beta-chain gene, indicating that non-T non-B/LGL cells may also be involved in the regulation of the class-specific antibody responses. It appears that, in human as well as murine systems, T- and NK-cells have the capacity to co-express multiple class-specific FcRs and to produce the corresponding immunoglobulin binding factors. While the Fc epsilon R expression is abnormally enhanced in the diseases with hyperimmunoglobulinemia E, disregulation of Fc alpha R is associated with certain human diseases involving the altered IgA regulation. In IgA nephropathy, which is characterized by increased serum IgA level and IgA deposition in the mesangium, there is an enhancement of the expression of Fc alpha R. In contrast, IgA failed to induce Fc alpha R significantly on the lymphocytes from the patients with selective IgA deficiency, indicating that Fc alpha R plays an important role in the IgA regulation in vivo.

Animals↗
PubMed1987

Derivation of a murine monoclonal antibody useful for immunohistochemical diagnosis of human adenoid cystic carcinoma and stomach cancer.

A monoclonal antibody, NCC-SG-007, was raised with a formalin-fixed adenoid cystic carcinoma of the salivary gland as the immunogen. The reactivity of the antibody was tested on paraffin sections using the avidin-biotin-peroxidase complex (ABC) method. NCC-SG-007 reacted with 36% of the adenoid cystic carcinomas, 42% of the pleomorphic adenomas and 14% of the mucoepidermoid tumors of the salivary gland which were tested. The antibody also reacted with some normal tissues and various other tumors. Surprisingly, NCC-SG-007 showed a reactivity as high as 94% with gastric carcinomas. The antigenic determinant was revealed to be a carbohydrate chain with no terminal sialic acid, the molecular weight of which was estimated to be 1.5 X 10(6) daltons. This antigen differed from other previously reported gastrointestinal cancer-associated antigens. The antibody should be useful for studying adenoid cystic carcinomas of the salivary gland and gastric cancers.

Animals↗
PubMed1987

Characterization of osteoblast precursor cells isolated from colchicine treated rats.

The previous study showed that an unusual mineralized tissue appeared in the bone marrow of rats 5 days after colchicine injection. The bone marrow stroma cells isolated from the colchicine treated rats were cultured and characterized to elucidate the relationship between the bone marrow stroma cells and the unusual mineralized tissue. The bone marrow cells were obtained 1 to 5 days after the injection of colchicine and the attached cells (marrow stroma cells) were cultured and tested for alkaline phosphatase (ALP) activity as a marker of osteoblastic characteristics. The cultured cells isolated 2 days after the injection (Col. 2 cells) showed a low ALP activity during the initial culture period. However, the activity began to increase and showed a high level from the 14th to the 30th day of culture. The other cell populations did not show an increase of ALP activity during the culture period. The ALP of the Col. 2 cells resembled the bone type enzyme by their biochemical characteristics. The Col. 2 cells became to respond to both the parathyroid hormone and prostaglandin E2 during the late stage of culture period. These findings suggest that the bone marrow stroma cells isolated from the rats 2 days after the colchicine injection become enriched with osteoblast precursor cells. These cells might differentiate into the osteoblasts during the repair process following the severe damage after the colchicine injection and produce an unusual mineralized tissue in the bone marrow cavity.

Acid Phosphatase↗
PubMed1987

Serological and immunohistochemical detection of a gastrointestinal cancer-associated asialoglycoprotein antigen of human with a murine monoclonal antibody.

A murine monoclonal antibody (mAb), NCC-AS 13 (IgM, kappa), was produced after immunization with ascites from a gastric cancer patient. In a sandwich enzyme immunoassay, NCC-AS 13 reacted with the sera from 32% of 58 gastric cancer patients, 44% of 9 hepatocellular cancer patients and 33% of 6 colorectal cancer patients. Immunohistological analysis showed NCC-AS 13 to react with approximately 90% of 51 gastric carcinomas, 12 out of 12 colon carcinomas and 3 out of 4 lung carcinomas. The NCC-AS 13 defined antigen was determined to be a carbohydrate without terminal sialic acid on macromolecular glycoproteins. This mAb NCC-AS 13, detecting a novel antigen associated with gastrointestinal cancers, may have potential uses for tumor immunoscintigraphy and for the therapy of gastric cancer patients.

Antibodies, Monoclonal↗
PubMed1987

Colonoscopic polypectomy as a tool for management of colonic polyps and detection of new lesions.

Over the past 14 years 1,000 polyps were removed via colonscope and histologically examined. There were 869 neoplastic polyps including 125 carcinomas. Non-neoplastic polyps included 32 inflammatory polyps, 32 metaplastic polyps, 27 juvenile polyps and 17 Peutz-Jeghers type polyps. Ninety-eight focal carcinomas were adequately treated by polypectomy only, whereas 9 out of 26 invasive carcinoma required further operations with one residual carcinoma in the pararectal tissue and colonic wall respectively. The criteria requiring additional surgery in invasive carcinoma are 1) lymphatic permeation of the submucosa, 2) poorly differentiated carcinoma or 3) massive invasion close to the cut end. Not only polypoid adenomas but also flat adenomas were found to exist and seem to play an important role in the adenoma-carcinoma sequence. It became clear that colonscopic polypectomy was a useful tool for the management of colonic polyps.

Adenoma↗
PubMed1987

[The value of follow-up bone scans in the management of postoperative breast cancer--when should it be performed?].

We have reviewed the bone scans of 106 patients with breast cancer taken after surgery, in order to determine when follow-up bone scans should be performed. The results obtained are as follows: 1. The incidence of bone metastasis was seen to be 11.6% in stage I, 39.4% in stage II, 43.5% in stage III, 57.1% in stage IV, and 30.2% overall; 2. Of 32 patients with bone metastasis, 28 patients (87.5%) developed bone metastases within the first 4 years; 3. The scan conversion rate was found to be 31.9% and the time interval to conversion ranged from 4.5 months to 28.5 months; and, 4. The period from single to multiple metastases ranged from 2 months to 37.5 months. Our findings suggest that postoperative follow-up bone scans for stage I breast cancer should be performed annually or when symptoms develop, and follow-ups for stages II, III and IV at six monthly intervals for the first 4 years after surgery and then annually.

Bone Neoplasms↗