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M A Swerdlow

Publications and source records attributed to M A Swerdlow.

14 recordsLinked to original sources
PubMed1988

Crystalline inclusions associated with lung adenomas in C57BLxC3H F1 mice.

In a study on lung adenomas induced by the administration of a single intragastric dose of sodium thiocyanate, diethylnitrosamine, or its precursors to 15-day-old C57BLxC3H F1 mice the occurrence of crystalline inclusions in pulmonary parenchyma was noted. A correlation between crystalline deposits and number and type of adenomas seen between 46 and 110 weeks was observed. In 45/61 adenomas, eosinophilic cells containing inclusions were seen scattered in the pulmonary tissue, adjacent to tumor areas, in desquamated cells in the bronchioles, in the lumens of the submucosal bronchial glands, and within the alveoli. By contrast 7/85 mice without adenomas showed inclusions (4/7 mice had pneumonitis). The inclusions stained with bromophenol blue, were PAS positive diastase resistant and showed varying amounts of alpha-1-antitrypsin, IgG, and IgA by immunoperoxidase technique. Ultrastructure revealed membrane bound rods with a lattice pattern.

Adenoma↗
PubMed1987

Neonatal lupus with congenital atrioventricular block and myocarditis.

This is a case of a child with neonatal lupus and congenital atrioventricular (AV) block, born to a mother with asymptomatic, systemic lupus erythematosus (SLE). The child, despite pacemaker insertion, died of septicemia and myocarditis at the age of three months. Although the association of neonatal lupus with congenital AV block is well-recognized, there are only few pathologic studies of the conduction system reported in the literature. This is such a study in which we emphasize that, due to an altered immune system in the child, septicemia may be the cause of death in some cases.

Female↗
PubMed1986

Induction of neoplastic lesions in the livers of C57BL x C3HF1 mice by chloral hydrate.

Chloral hydrate is a compound of environmental significance. The current investigation was undertaken to evaluate the carcinogenic effect of chloral hydrate, because it is present in drinking water and it is also used as a sedative. Fifteen-day-old C57BL x C3HF1 male mice were given a single dose of chloral hydrate in distilled water at two dose levels: group 1, 5 micrograms/g BW; group 2, 10 mu/g BW (20-25 mice per group). Thirty-five mice given distilled water only served as controls. Animals were sacrificed at 24 hr and thereafter at various intervals up to 92 weeks. The entire liver was fixed and examined histologically. Mice sacrificed between 48 and 92 weeks showed hepatic lesions ranging from hyperplastic to trabecular carcinomas. The tumor incidence in mice given 10 micrograms/g chloral hydrate (six of eight) was significantly higher (P less than 0.05) than the incidence in the controls (two of 19). These findings indicate that chloral hydrate should be more thoroughly studied for potential carcinogenicity.

Animals↗
PubMed1984

IgA deposition in liver in alcoholic liver disease. An index of progressive injury.

A retrospective study was done to evaluate the prognostic importance of the patterns of IgA deposition in the liver in alcoholic liver disease. The patterns of IgA deposition in the liver were determined by direct immunofluorescence with fluorescein conjugated rabbit antihuman IgA. Twenty of 40 patients showed a characteristic continuous pattern, and 20 patients showed the nonspecific discontinuous pattern of IgA deposition in the liver. Alcoholic liver disease progressed considerably in 14 (70%) of the 20 patients with an initial continuous pattern and in three (15%) of the 20 patients with an initial discontinuous pattern. Alcoholic liver disease did not progress in six (30%) of 20 patients with an initial continuous pattern and in 17 (85%) of the 20 patients with an initial discontinuous pattern of IgA deposition in the liver.

Fatty Liver, Alcoholic↗
PubMed1983

IgA subclasses in liver tissues in alcoholic liver disease.

Examination of liver biopsy specimens from 59 patients with alcoholic liver disease and 21 nonalcoholics by immunofluorescence and immunoperoxidase methods using fluorescein-conjugated and peroxidase-labeled antisera against IgA, IgA1, IgA2, and S-IgA showed that 47 of 59 biopsy specimens from alcoholics and 0 of 21 from non-alcoholics showed a "continuous" pattern of IgA and IgA-subclass deposition (P less than 0.001). Grading the intensity of immunofluorescence on 1-4 scale, biopsies with the continuous pattern showed grade 3-4 activity against IgA2 and S-IgA and only grade 1-2 activity against IgA1. Biopsies with the discontinuous pattern showed only grade 1-2 activity against S-IgA, IgA2, and IgA1. Grade 3-4 activity was persistent in all the 47 specimens with the continuous pattern, despite pretreatment with blocking anti-IgA1, whereas 20 and 38 biopsies showed the same activity after blocking with anti-IgA2 and anti-S-IgA sera, respectively. It is concluded from these studies that IgA2 subclass formed a major subclass component contributing to the continuous pattern of IgA deposition in hepatic tissues and that the major source for this IgA in alcoholics was probably derived from the gastrointestinal tract.

Fluorescent Antibody Technique↗
PubMed1983

IgA deposits in skin in alcoholic liver disease.

IgA deposition in the liver in a characteristic continuous pattern is a consistent finding confirming the diagnosis of alcoholic liver disease. This study demonstrates a correlation between characteristic IgA deposition in the liver and IgA deposition in and around superficial dermal capillaries in alcoholic liver disease. Simultaneous liver and skin biopsies from nineteen patients with established alcoholic liver disease and eight patients with nonalcohol-related diseases of the liver were studied using standard immunofluorescent and immunoperoxidase methods. Fourteen of the nineteen alcoholic patients showed the characteristic pattern of IgA deposition in the liver, twelve of these fourteen patients showed IgA deposits in and around superficial dermal capillaries, and of these twelve patients, nine showed concurrent deposits of C3 in the skin capillaries. None of the eight nonalcoholic control patients showed either characteristic liver IgA deposits or any dermal deposits. Biopsy of clinically normal skin can be of adjunctive aid in the confirmation of alcoholic liver disease.

Biopsy↗
PubMed1982

Patterns of IgA deposition in liver tissues in alcoholic liver disease.

Observations in 136 liver biopsies from patients with alcoholic and nonalcoholic liver diseases reveal that IgA deposition in liver tissues appears to have a high degree of morphologic specificity for alcohol injury. Using a direct immunofluorescence technic with fluorescein-conjugated anti-IgG, anti-IgA, anti-IgM, and anti-C1q, four different staining patterns are recognized. These are labelled as "continuous," "discontinuous," "granular," and "pericellular" types depending on their morphologic characteristics and distribution patterns. Fifty of 64 biopsies from alcoholics showed a "continuous" pattern of anti-IgA activity while only three of 72 biopsies from nonalcoholics showed a similar pattern (P less than 0.001). A "pericellular" pattern of anti-IgA activity appears to indicate a more aggressive behavior of alcoholic liver disease. "Continuous" and "pericellular" patterns are seen in "chronic active hepatitis of alcoholics" but not in chronic active hepatitis in nonalcoholics. Anti-IgM activity appears to indicate chronicity of the disease process but does not have any specificity.

Biopsy, Needle↗
PubMed1980

Postirradiation malignant fibrous histiocytoma of the lung. Demonstration of alpha 1-antitrypsin-like material in neoplastic cells.

A metastasizing fibrous histiocytoma arising in the lung of a patient who received radiation therapy and long-term chemotherapy for malignant lymphoma is presented. Ultrastructural studies revealed fibroblast-like and histiocyte-like cells, cells of intermediate type showing ultrastructural features of both fibroblast-like and histiocyte-like cells, primitive mesenchymal cells, multinucleate tumor cells, and xanthomatous cells. The neoplastic cells showed dilated rough endoplasmic reticula with intracisternal accumulation of electron-dense material forming lattice-like structures. Direct immunofluorescence staining of the neoplastic cells using antihuman alpha 1-antitrypsin showed specific activity, with fluorescent deposits exhibiting interlacing globular formations. These findings and their implications are discussed.

Female↗
PubMed1976

Ultrastructure of the basal cell adenoma of parotid gland.

Electron microscopic examination of two classical examples of so-called basal cell adenoma of parotid gland disclosed four distinct cellular types: the squamous epithelial cells with tonofilaments and prominent desmosomes predominantly located at the central portion of neoplastic mass; the basally located secretory cells with numerous secretory granules; the occasional intermediate cells with scanty cytoplasmic microfilaments; and the peripherally situated attenuated myoepithelial cells. The neoplastic clusters are surrounded by highly replicated basal laminae with microfibrils in their interstices. This information about its component cells suggests that basal cell adenoma arises from the secretory duct, in particular the intercalated duct, of the parotid gland. The term basal cell adenoma appears appropriate for its designation. It is interesting that the secretory cells and the multilayered basal laminae illustrated in the present study are reportedly seen in adenoid cystic carcinoma of the salivary gland; this finding would suggest a common cellular origin for these two neoplasms.

Adenoma↗
PubMed1976

Comparative ultrastructure of tubular carcinoma and sclerosing adenosis of the breast.

The innocuous histologic appearance of tubular carcinoma of the breast and its superficial histologic resemblance to sclerosing adenosis will occasionally present diagnostic problems. A comparative ultrastructural analysis of two tubular carcinomas and three cases of sclerosing adenosis was made that showed definite differences in the pattern of myoepithelial cell differentiation and basal lamina deposition in these two entities. Prominent myoepithelial cells and basal lamina reduplication were both conspicuous features of sclerosing adenosis that appeared to be absent in tubular carcinoma. Intracytoplasmic "pseudocysts" were frequently found in sclerosing adenosis, but not in tubular carcinoma. Conversely, intracytoplasmic lumina and incomplete tubular structures were present in tubular carcinoma and seemingly absent in sclerosing adenosis. Such basic ultrastructural differences may help to differentiate these two mammary lesions when diagnostic problems occur at the conventional light microscopic level.

Breast Diseases↗
PubMedDate not supplied

The autopsy.

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Attitude of Health Personnel↗