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M A Savina

Publications and source records attributed to M A Savina.

At least 19 recordsLinked to original sources
PubMed2006

[Diversity of wild and domestic pig populations estimated by a set of serum allotypes].

Variation of serum protein allotypes serving as genetic markers of the blood has been analyzed in 29 populations of the domestic pig and subspecies of the wild boar. The population biodiversity and genetic structure have been estimated by two methods: by the frequencies of allotype combinations and with the use of a map constructed in the space of two principal components. The results obtained are the basis for determining the characteristics of the microevolution of wild boars and formation of the breeds of domestic pigs.

Animals↗
PubMed2002

[Age variation of the phenotypic expression and the inheritance of the IgG system of immunoglobulin allotypes in the pig].

The specificity of phenotypic expression and inheritance of immunoglobulin allotypes IgG1a and IgG2b in pigs are discussed. It was shown that a negative state by both these allotypes is repeatedly found among newborn pigs but is extremely rare in pigs older than one month. A model, which simultaneously describes the genetic determination of allogroups formed by allotypes IgG1a and IgG2b and the dynamics of ontogenetic expression of individual genotype by system IgG with a result of a visually registered phenotype, was developed. Allele frequencies by system IgG were assessed in populations of domestic pigs of productive breeds, laboratory miniature pigs, and wild Eurasian pigs.

Age Factors↗
PubMed2001

[A2G--a new system of alpha-2-globulin allotypes in pig blood serum].

Combinations of four alpha-2-globulin allotypes were studied for their distribution in pigs of nine different breeds and hybrid groups. Based on this analysis, a new, previously unpublished polyallelic genetic system designated A2G was postulated. The complex alleles of this system control alpha-2-globulin allotypes and are suggested to be encoded by genes of two subloci. One of these subloci is virtually monomorphic, whereas the other has at least four allelic variants.

Alleles↗
PubMed2001

[Characteristics of some breeds of swine and populations of Georgian and Western Siberian wild boar by immunogenetic systems of blood serum proteins].

A bank of reagents for hog serum protein allotypes has been created. All of these allotypes passed international comparison tests in 1987-1988. The bank can be used for typing the animals for four generally accepted (Gp, LpB, Lpr, and IgGH) and several experimental systems. In this study, immunogenetic characteristics of some pig breeds (Large White, Lithuanian White, Swedish Landrace, Kakhetinskaya, and Svanetskaya) bred in Georgia are compared with those of western Siberian breeds (Large White, Kemerovskaya, and Northern Siberian), and some foreign breeds, as well as with European, Caucasian, and Siberian subspecies of the wild boar.

Animals↗
PubMed2000

[Dynamics and genetic determination of transformation of alpha macroglobulins allogroups in different populations of domestic pigs].

The results of genetic studies of transformation of alpha-macroglobullin (AM) allogroups (i.e., fixed allotype combinations) in pigs are presented. Population and hybridization studies showed that the phenomenon discovered was based on activation/inactivation of the AMI allotype expression, which depended on the genetic environment and interlocus relationships. Genetic and selection bases, as well as the significance of the transformation phenomenon described, are discussed.

Alleles↗
PubMed2000

[Interaction of polymorphic systems in a population of Semirechenskaya swine breed].

Associations between the alleles of nonlinked polymorphic genetic systems were studied in the population of the Semirechenskaya breed of pigs. The study of marker pairs of 21 genetic systems revealed that 7% of associations are statistically significant. Most of the associations detected are assumed to arise from breeding by commercial productivity traits and from the formation of an integral genotype adapted to conditions of artificial selection in the population.

Animals↗
PubMed1999

[The association of genetic markers in two related swine breeds].

The combinations of genetic markers (allotypes, blood-group systems, and polymorphic proteins) were analyzed in pigs of two phylogenetically related Siberian populations: the newly selected Siberian specialized meat breed SM-1 (daughter breed) and the reproductive core of the Kemerovo breed (maternal breed). Statistically significant interlocus associations were found between the genetic markers that are known not to be linked, being assigned to different linkage groups, and the main selected meat-productivity traits. Some of these associations were shared by both breeds; others were found to be specific to either maternal or daughter populations. Possible genetic mechanisms underlying these associations and their significance are discussed.

Animals↗
PubMed1997

[Further research on the immunogenetic G blood group system in swine].

As a result of several cycles of isoimmunization, two variants of antisera dose-dependently reacting with antigens of the porcine G blood group were obtained. It is suggested that the specific response of immunized pigs to antigens inherited from different wild boar subspecies may be associated with allele Ga and probably with Robertsonian translocations 15/17 and 16/17.

Animals↗
PubMed1996

Regulation of glutamic acid decarboxylase mRNA expression in rat brain after sertraline treatment.

We now investigated the effect of chronic treatment with sertraline on glutamic acid decarboxylase mRNA expression in different rat brain areas by means of in situ hybridization. We found a reduced glutamic acid decarboxylase mRNA expression in the prefrontal cortex, accumbens nucleus, olfactory tubercle and reticular nucleus of the thalamus. The involvement of presynaptic modulation of gamma-amino-butyric acid transmission in the anxiolytic effect of sertraline is discussed.

1-Naphthylamine↗
PubMed1991

[The forms of the intercellular contacts of Leishmania in culture].

Two kinds of intercellular interactions have been observed in cultures of 12 investigated Leishmania species (L. major, L. tropica, L. donovani, L. infantum, L. sp. ZMA, L. mexicana, L. hertigi, L. braziliensis, L. tarentolae, L. adleri, L. gymnodactyli, L. gulickae). The first kind looks as adhesion of two specimens with their fore-ends. This way is characteristic of promastigotes of different morphotypes as well as of the interphase and dividing organisms to be most frequently seen in L. mexicana and L. gymnodactyli, and in both dark and lucid forms. The second kind of intercellular interactions involves a coupled adhesion of morphologically similar promastigotes with free ends of flagella. It is most characteristic of L. gymnodactyli and specially of dark promastigotes. Proves are provided that both the kinds of cell interactions are not associated with cell division, that they may only partially be connected with the phenomenon of rosette formation, and that they represent different phenomena. It is supposed that the intercellular contacts with the fore-ends may reflect gene exchanges in two partners, with a possible involvement of the kinetoplast DNA.

Animals↗
PubMed1991

[Homology between the Lpm system of allotypes in the American mink and the Gp system of allotypes in the domestic pig].

The 5 alpha-macroglobulin allotypes alpha M1, alpha M2, alpha M3, alpha M4 and alpha M5 were identified in pig. The alpha M1 allotype was reported as a marker of pig alpha-macroglobulin, the latter being homologous to alpha 2-macroglobulins in human and in mink. The allotypes alpha M2-alpha M5 were specified as markers of the second isotypical variant of pig alpha-macroglobulins, which was homologous to mink Lpm macroglobulin (alpha 1M). As seen from data obtained by International Comparative Test ISABR 87-88, alpha M1 is a new allotype, while allotypes alpha M2--alpha M5 correspond to four allotypes in the Gp system (Janik et al.). Based on these data, a conclusion was made on the homology between the Lpm system in american mink and the Gp system in pig. Since the allotypes studied are the part of alpha-macroglobulins, a locus controlling them was designated the AM locus. We also find it more advantageous to apply the same name to the homologous locus in mink, instead of the Lpm used earlier. Genetic control of 5 allotypes was studied and the structure of the AM locus in pig analysed in detail. Comparative study of organization of the above locus and the homologous locus in mink was carried out.

Animals↗
PubMed1990

[The results of international comparative testing of reagents to swine serum protein allotypes conducted in 1987-1988].

Antisera to 15 allotypes of pig serum protein were raised and compared with those prepared elsewhere, within the framework of International Comparison Test of pig blood groups and polymorphic proteins and enzymes in 1987-1988. Of 15 allotypes tested 8 were found to coincide with the known GpB, GpD, GpA, Gpa, IGH3 C1, Lpb3, Lpb12, Lbr1. For 7 allotypes left, no analogues were found out. They seem very likely to be new allotypes of pig serum proteins.

Animals↗
PubMed1988

[Allotypes of swine serum proteins].

A series of intraspecific immunizations of pigs by whole blood serum in complete Freund's adjuvant was performed. Four allo-antisera to different serum allotypes were obtained. These allotypes were assigned to A1, B1, C1 and D1. The results of identification show that the allotype A1 is probably a marker of immunoglobulin G. The allotypes B1, C1 and D1 were established to belong to different alpha-globulins, D1 being a marker of lipoprotein. Two geographically isolated populations of white large breed were tested. Two allotype combinations were found to be independent of one another and of sex. Significant differences in allotype frequency between two populations were only established for the G1 (F = 0.142 and 0.334; P less than 0.01). In both populations allotype B1 has the frequency 1.0, the frequencies of allotype A1 being 0.734 and 0.747. Most rare is the allotype D1 (F = 0.078 and 0.082).

Alleles↗
PubMed1987

[Genetics and evolution of the Lpm-system in American mink. VI. Identification, genetic control and phylogenesis of the new allotype Lpm13].

Data on immuno- and biochemical identification, genetic control and phylogenesis of new allotype Lpm13 of the Lpm system in domestic mink are presented. This allotype is encountered in mink populations with the frequency 0.9 and higher. The availability of Lpm13 genetic marker permitted another haplotype to be revealed, in addition to the eight known Lpm haplotypes by means of genetic analysis. It was established that, alongside with the earlier described haplotype Lpm3,4,6,8,9,10,11 (abbreviation H3), there exists a similar haplotype, Lpm3,4,6,8,9,10,11,13 (abbreviation H3.13), containing the Lpm13 gene. Of the rest seven haplotypes, five have the Lpm13 gene and two do not. Taking into account this gene and corresponding antigenic marker, the differentiation of 28, instead of 25, phenotypes and 45, instead of 36, genotypes for the Lpm system became possible. Lpm13 antigenic specificity was found with no exception in all individual serum samples taken from ten species and interspecific hybrids of Mustelidae which are closely related to domestic mink. The data obtained give grounds to refer the newly identified Lpm13 gene to the first evolutionary conservative category of genes of the multigenic Lpm system which is also represented by the Lpm6, Lpm9, Lpm10 and Lpm11 genes. The hypotheses of instantaneous formation of polymorphism of the Lpm system in domestic mink are briefly regarded.

Animals↗
PubMed1987

[Differentiation of Leishmania in culture].

During their cultivation on diphasic medium Leishmania donovani, L. major and L. gymnodactyli pass through the regular differentiation within the promastigote stage of the life cycle that involves a gradual change of the four main morphological forms: 1) extremely basophilic, short, wide, actively dividing cells; 2) lightening longer forms with pointed posterior ends; 3) long, cigar-shaped uniformly light specimens with the clear nucleus in the central or posterior position and distinct karyosome; 4) small narrow nearly non-basophilic cells with a long compact nucleus and a rather large kinetoplast. The latter are similar, on the one hand, to the final stage of Leishmania development in Phlebotomus and, on the other hand, to metacyclic forms of Trypanosoma cruzi infective for the vertebrate host.

Animals↗
PubMed1981

Immunogenetic study on the polymorphism of serum alpha 2-lipoproteins in mink. IV. Diallelism at the Ld locus of low-density lipoprotein.

Antibodies against a new allotype, Ld2, of mink low-density lipoprotein (LDL) were obtained by alloimmunization with a preparation of this lipoprotein. The two known allotypes of LDL, designated Ld1 and Ld2, are coded for by codominant alleles of the autosomal Ld locus. This locus is probably involved in the genetic control of the whole serum pool of LDL molecules. In Ld1/Ld2 heterozygotes, LDL is represented by two homozygous types of molecules, Ld1 and Ld2; it has no hybrid molecules bearing both allotypic specificities together. The results suggest that the Ld locus has, presumably, only two alleles in the mink populations studies. Mink LDL having allotypes Ld1 and Ld2 was found to be homologous to human and pig LDLs. Antigenic specificity of Ld1 allotype was established in the sera of a wide phylogenetic range of mammals and in the human LDL. The parallelism between the phylogenetic antiquity of the Ld1 gene and its high frequency in mink and other species may be attributed to the selective value of this gene, which has been retained unaltered during macroevolution.

Alleles↗