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Biomedical subjects

M A Price

Publications and source records attributed to M A Price.

At least 55 records · Page 3Linked to original sources
PubMed1982

Cell calcium in human peripheral blood lymphocytes and the effect of mitogen.

The apparent cell concentration of calcium in human peripheral blood lymphocytes is 143.3 +/- 17.7 microM, as measured by two different techniques using 45Ca. The steady-state level of accumulation, and possibly the rate of uptake, are increased in the presence of succinyl-concanavalin A. Initiation of the mitogen-induced alteration of cell calcium occurs within 1-2 min and the change is complete within 5-10 min. Determinations of cell calcium in cells suspended in low Na media indicate that (1) there is no difference in cell calcium between cells incubated in 142 mM extracellular Na and cells incubated in 63 mM extracellular Na, and (2) the mitogen-induced increase in cell calcium is unaffected by a decrease in extracellular sodium concentration (to 63 mM).

Calcium↗
PubMed1979

Enhancement of parathyroid hormone-stimulated bone resorption by poly-L-lysine.

Poly-L-lysine (PL II; mol wt, 1000-4000) was added to fetal rat bones cultured in a chemically defined medium (BGJ) containing bovine serum albumin in the presence and absence of parathyroid hormone (PTH). Bone resorption was measured by the release of previously incorporated 45Ca. The addition of PL II at concentrations of 3-100 microgram/ml enhanced the stimulation of bone resorption by submaximal doses of PTH but had little effect on 45Ca release from control unstimulated cultures. Higher concentrations of PL II produced inhibition of 45Ca release. Dialysis of PL II did not alter enhancement or inhibition by PL II. PL II did not increase sensitivity to PTH in serum-supplemented cultures. Higher molecular weight PL II preparations were less effective. PL II did not enhance the resorptive response to 1,25-dihydroxyvitamin D, prostaglandin E2, osteoclast-activating factor, or bacterial endotoxin. The mechanism of the selective ability of PL II to enhance the response to low concentrations of PTH is unknown but may be due to the ability of this basic polypeptide to interfere with binding of PTH to sites other than the hormone receptor or to block degradation of PTH by bone.

Animals↗
PubMed1976

Geographical distribution of amyloidosis in Papua New Guinea.

During an 11 year period 73 cases of amyloidosis were diagnosed at Port Moresby General Hospital as a result of histological examination of autopsy or biopsy material. No case of amyloidosis came from either the Highlands or North Coast regions although these two areas together accounted for 16% of the medical admissions at the hospital and made up a similar percentage of Port Moresby's Papua New Guinean population. Within the sub-district in which Port Moresby is situated there was a much higher incidence of amyloidosis in the inland, more sparsely populated, census divisions of the sub-district. Aetiological factors which may be related to the common occurrence of amyloidosis in Papua New Guinea are discussed in light of the observed geographical distribution of cases.

Amyloidosis↗
PubMed1976

Amyloid fibril protein AA in Papua New Guinean amyloidosis.

In this study of protein composition of amyloid fibrils isolated from eight patients representative of the spectrum of amyloidosis found in Papua New Guinea has been investigated. All fibril preparations, including three from patients with amyloidosis secondary to lepromatous leprosy and one from an unusual juvenile case of primary amyloidosis, contained the non-immunogobulin amyloid protein, protein AA. However, only 44% of thirty-six amyloid patients had detectable levels of the protein AA-related serum component, protein SAA. Alkali-degraded material from each of the fibril preparations failed to react in double immunodiffusion test with antiserum to the amyloid-related light chain VgammaV, but evidence was found for this immunoglobulin light chain-specificity in the serum of one patient.

Adult↗
PubMed1975

Association of amyloidosis with erythema nodosum leprosum reactions and recurrent neutrophil leucocytosis in leprosy.

Rectal biopsy in 190 inpatients at a leprosy hospital in the Highlands of Papua New Guinea disclosed 16 patients with secondary amyloidosis. This represented 20% of the patients who had had polar lepromatous leprosy (L.L.) for more than 2 years. Patients with amyloidosis characteristically had either a history of recurrent attacks of erythema nodosum leprosum (E.NH) reactions or chronic trophic ulcers. Levels of the serum component (protein SAA) antigenically related to the amyloid fibril protein AA were monitored, at varying intervals for three months, in lepromatous patients with E.N.L. reaction. The SAA levels rose during E.N.L. reactions in parallel with the neutrophil count. SAA occurred with greatest frequency among patients with LH, while most non-lepromatous patients with detectable SAA had chronic trophic ulcers. The correlation between raised neutrophil count and elevated SAA concentration, observed in this and other studies, suggests that neutrophils are associated with the production of SAA.

Amyloidosis↗