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Biomedical subjects

M A Madwar

Publications and source records attributed to M A Madwar.

At least 19 recordsLinked to original sources
PubMed1990

Correlation between circulating schistosomal antigen, schistosomal complement C3 and intensity of infection.

53.7% of the already parasitologically proved schistosomiasis cases showed circulating schistosomal antigen (C.S.A.) in their sera with significantly higher levels than the controls, with no correlation between the level of C.S.A. and the foecal egg count. Significant higher levels of schistosomal complement C3 were found in schistosomiasis patients as compared to the control group, with no significant difference in the mean level of C3 between patients with positive and negative C.S.A.

Adolescent↗
PubMed1989

Immunoglobulins in bilharzial patients with and without chronic hepatitis.

The mean values of IgG and IgA were significantly higher in bilharzial patients than in the non bilharzial ones. The mean IgG level in patients with S. mansoni was significantly higher than that in patients with negative stool while the reverse was true as regards IgA. Among the bilharzial patients the mean values of IgG, IgM and IgA were significantly higher in C.A.H. and C.A.H. bilharziasis than in patients with pure bilharziasis, C.P.H. and C.P.H. with bilharziasis. It might be concluded that the altered immune response produced by schistosomiasis, the frequent exposure to the hepatitis B. virus and the bilharzial hepatitis lesions, all may pave the way for development of severe diseases e.g. chronic active hepatitis and liver cirrhosis.

Chronic Disease↗
PubMed1989

Hepatic schistosomiasis and chronic active hepatitis.

As part of an investigation of the possible role of autoimmune mechanisms in the development of chronic active hepatitis (CAH) in schistosomiasis, sera from 25 patients (Group I) with hepatic schistosomiasis without histological features of CAH and 5 patients (Group II) with CAH and intestinal mansoniasis, but without discernible hepatic infection, were examined for the presence of the liver membrane antibody (LMA) that is reportedly associated with the autoimmune variant of CAH. All patients were HBsAg-negative. Circulating LMA was found in 17 (68%) Group I and 3 (60%) Group II patients. Low titres of various organ-non-specific autoantibodies were found in about a third of all patients but these did not correlate with the presence of LMA. There was also no correlation between LMA and antibodies to the hepatitis B core antigen (anti-HBc), found in the sera of 67% of the patients, indicating that occult or recent past hepatitis B virus infection was not a factor in LMA production. Long-term follow-up of the Group I patients should reveal whether current LMA-positivity is related to eventual development of CAH in hepatic schistosomiasis.

Adolescent↗
PubMed1989

Dot-ELISA in diagnosis of schistosomiasis.

One microliter of S. mansoni egg antigen was dotted directly on the nitrocellulose paper sheet acting as the adsorbent surface (9 dots/paper). The sera of 25 Egyptian patients and 15 healthy persons (2 microliters of each) were dotted over the antigen dots, then 2 ml of each of the blocking, washing, HRP-conjugated IgG and DAB adding procedures, were added over the nitrocellulose paper in the petri-dish at room temperature. An intact brown circle (by naked-eye) indicates a positive in Dot-ELISA. There is an insignificant dot colour intensities in different clinical stages of S. mansoni infected Egyptians whereas, a direct relation was obtained between egg count and the colour intensity of the dots. The test had 100% sensitivity and 86% specificity thus it appears to be useful for both laboratory and field studies.

Animals↗
PubMed1989

HBSAg antigenaemia in bilharzial patients.

The frequency of HBSAg in bilharzial patients (7%) was significantly higher than that in non bilharzial ones (2-5%). The presence of insulted liver by bilharzial, non bilharzial (C.P.H. and C.A.H.) or both (C.P.H. with bilharzia and C.A.H. with bilharzia) lesions have no role in changing the incidence of HBSAg in sera of bilharzial patients.

Egypt↗
PubMed1988

Circulating antigens for assessing cure in schistosomiasis mansoni.

An antigen capture enzyme-linked immunosorbent assay using antibodies made in rabbits and sheep to adult Schistosoma mansoni extract antigens has been developed and evaluated as a diagnostic test for schistosomiasis. 25 of 42 patients with S. mansoni infections had detectable circulating antigen while sera from all of 45 normal subjects and patients with other parasitic infections did not react. When the 25 with antigenaemia were retested 3 months after treatment, all 21 who were cured were negative while the 4 with persisting S. mansoni eggs in their stools remained positive. Antibodies to a fractionated egg antigen (CEF6) also diminished in most patients following successful treatment, but the reduction was insufficient to predict successful therapy.

Adolescent↗