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Biomedical subjects

M A Macedo

Publications and source records attributed to M A Macedo.

4 recordsLinked to original sources
PubMed1997

Factor analysis, a more accurate method to be used in epidemiological studies of blood pressure in children.

The aim of our study is to evaluate whether factor analysis is better able to explain the variability of systolic blood pressure (SBP) and diastolic blood pressure (DBP) when compared with regression analysis, which is the usual tool to study a set of variables related to blood pressure (BP). SBP, DBP, weight, height, BMI, triceps skin-fold, sexual maturation and rurality were studied in 889 children aged 5-18 years (389 boys and 500 girls). The proposed method transforms any set of variables into a set of new variables (factors) which are uncorrelated with each other. One of the factors obtained clearly explains the BP variance of the. With this method, the algorithm accepts all meaningful variables, while regressions reject most of them. This method also explains a larger amount of BP variability, losing as little information as possible. In our sample the percentage of the total variance (communality) explained by the three factors was 80.3% for SBP, 88.1% for DBP in males, 79.3% for SBP and 90.7% for DBP in females. For the same sample, regressions only explained 41.2% in males and 41.9% in females for SBP, 40.9% in males and 47.2% in females for DBP. In conclusion, this method is more accurate for epidemiological studies producing a better overall score than regression analysis, losing almost no information from the sample. Two important strengths of the proposed methodology are as follows. First, it yields a unique, easy to calculate and flexible cardiovascular index for children, thus circumventing the problem of making decisions based on two variables (SBP and DBP). Second, such an index is the result of a methodology where specific BP variability is isolated rather than explained.

Adolescent↗
PubMed1976

Bacterial mesosomes. Real structures or artifacts?

The ultrastructural study of membrane organization in gram-positive bacteria related to the OSO4 fixation conditions revealed that large, complex mesosomes are observed only when the bacteria are subjected to an initial fixation with 0.1%OSO4 in the culture broth, as in the prefixation step of the Ryter-Kellenberger procedure. Evidence was obtained suggesting that the large mesosomes are produced by this prefization. The kinetic study of the membrane morphological alterations occurring during the prefixation of Bacillus cereus with 0.1%OSO4 in the culture broth showed that the amount of mesosome material increases linearly from zero to a maximum observed at 1.7 min of prefixation and that at about this time a maximum is reached for the number of mesosomes per unity of cell area and for the average individual mesosome area. The large mesosomes observed in gram-positives fixed by the complete Ryter-Kellenberger procedure would be the result of the membrane-damaging action of 0.1%OSO4. Such damaging action was deduced from the observation thay 0.1%OSO4 quickly lyses protoplasts and induces a quick and extensive leakage of intracellular K+ from B. cereus and Streptococcus faecalis. In support of that interpretation is the observation that in bacteria subjected to several membrane-damaging treatments, mesosome-like structures are seen after three different fixation procedures. In bacteria initially fixed with 1% OSO4, 4% OSO4 or 2.5% glutaraldehyde, no large complex mesosomes are observed, small and simple invaginations of the cytoplasmic membrane being present. The size of these minute mesosomes is inversely proportional that causes of fixation. Uranyl acetate was found among the studied fixatives the one to the rate the least damage to bacterial membranes. This fixative satisfactorily preserves protoplasts. In bacteria initially fixed with uranyl acetate no mesosomes were found. The results of the present work throw serious doubts on the existence of mesosomes, both large and small, as real structures of bacterial cells. It is proposed that a continuous cytoplasmic membrane without infoldings (mesosomes) would be the real pattern of membrane organization in gram-positives.

Bacillus cereus↗
PubMed1976

Effects of phenethyl alcohol on Bacillus and Streptococcus.

The activity of phenethyl alcohol (PEA) on Bacillus cereus, B. megaterium, and Streptococcus faecalis was studied by electron microscopy of thin sections and by the assay of intracellular K+ leakage. S. faecalis was unaffected by PEA at concentrations up to 0.5%, B. cereus was severely damaged by 0.5% PEA, and B. megaterium behaved intermediately. Important membrane ultrastructural alterations were observed in B. cereus cells treated with 0.5% PEA, namely the change in the geometry of the membrane profile from asymmetric to symmetric, the occurrence of prominent, complex mesosome-like structures, and membrane fracturing and solubilization. Protoplasts from B. megaterium were found to be quickly lysed by 0.5% PEA due to the disruption of the cytoplasmic membrane. The electron microscopic observations, together with the results of the study of the K+ efflux from B. cereus and B. megaterium, indicate that PEA primarily and directly damages the cytoplasmic membrane of sensitive bacteria. The breakdown of the permeability barrier probably is responsible for the observed bactericidal action of 0.5% PEA on B. cereus.

Bacillus cereus↗