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Biomedical subjects

M A Kelly

Publications and source records attributed to M A Kelly.

At least 127 records · Page 7Linked to original sources
PubMed1983

High pressure liquid chromatographic detection of intracellular retinoid binding proteins from cultured cell and tumor cytosols.

We report the first application of high pressure liquid chromatography (HPLC) in the rapid detection of cellular retinoic acid binding protein (CRABP) and cellular retinol binding protein (CRBP). Cytosols from cultured cells (3T6 and MCF-7) or from tumors (melanoma and ovarian) were labeled with [3H]retinoic acid (30 Ci/mmol) and [3H]retinol (43 Ci/mmol) and analyzed via HPLC employing a 60 cm TSK 3000 sw column. In each case CRABP and CRBP were readily detectable at an elution volume of 22.5 ml, consistent with their molecular weights of 14,600. Identity of the binding protein peaks was established by saturability, specificity, and selective inhibition of binding by an organomercurial. Thus, this method, which resolves CRABP and CRBP in crude mixtures from the majority of cytosolic proteins, should be a valuable tool in the evaluation of vitamin A-binding protein interactions and their biological significance.

Animals↗
PubMed1983

Continuous monitoring of mixed venous oxygen in neonatal pulmonary hypertension.

The management of persistent fetal circulation (PFC) in 3 neonates was facilitated by continuous monitoring of either pulmonary artery (PAP) or right ventricular pressure using an umbilical catheter with a catheter-tip electrode. The catheter was inserted without fluoroscopy via the umbilical vein, using continuously monitored oxygen tension and pressure waveform to determine entry into the right ventricle in 1 patient and the main pulmonary artery in 2 others. Right ventricular and PAP responses to dopamine, dobutamine, and tolazoline facilitated pharmacologic management, whereas continuous mixed venous oxygen tension (PVO2) provided an index of tissue oxygenation.

Catheters, Indwelling↗
PubMed1983

Human factor IX inhibitors: immunochemical characteristics and treatment with activated concentrate.

Plasma was obtained from two patients with severe factor IX deficiency who had developed specific inhibitors of factor IX. Immunochemical characterization of the inhibitors by coagulation inhibitor neutralization assays and by immunoelectrophoretic methods demonstrated that both were IgG antibodies. One of the antibodies appeared to be monoclonal in origin with IgG subclass 4 heavy chains and lambda light chains. The other appeared to be oligoclonal and contained IgG subclass 1 and subclass 4 heavy chains and kappa and lambda light chains. One of the patients was treated with conventional, non-activated factor IX concentrate and with activated factor IX concentrate (Feiba) for repeated bleeding episodes. Administration of Feiba resulted in a progressive shortening of the kaolin cephalin clotting time and was followed by a good clinical response. Infusion of non-activated factor IX concentrate failed to induce clinical resolution of haemarthroses and had minimal effect on laboratory tests. The presence of circulating immune complexes could not be demonstrated in this patient.

Adult↗
PubMed1983

Schistosoma mansoni adult microsomal antigens, a serologic reagent. II. Specificity of antibody responses to the S. mansoni microsomal antigen (MAMA).

The purified Schistosoma mansoni adult microsomal antigen, MAMA, was used in the quantitative single-tube kinetic dependent enzyme-linked immunosorbent assay (k-ELISA) to measure antibody levels of various human patient sera. The 511 serum specimens tested were from patients with both homologous and heterologous infections. Sera from U.S., Egyptian, Brazilian, and Puerto Rican patients infected with S. mansoni reacted strongly with MAMA. Chinese patients infected with S. japonicum, and Nigerians or Egyptians infected with S. haematobium produced much lower responses to this antigen than those infected with S. mansoni. Sera from patients with echinococcosis, filariasis, paragonimiasis, clonorchiasis, trichinosis, amebiasis, and hepatitis and from healthy uninfected control individuals generally contained no detectable antibodies against this antigen. The S. mansoni adult microsomal antigen, MAMA, therefore, appears to be a highly potent and specific reagent for the serodiagnosis of S. mansoni infections.

Animals↗
PubMed1983

Schistosoma mansoni adult microsomal antigens, a serologic reagent. I. Systematic fractionation, quantitation, and characterization of antigenic components.

A systematic and quantitative search was conducted to identify and isolate a serologically pertinent antigen with high specific activity and low cross-reactivity from adult worms of Schistosoma mansoni. Adult worms of S. mansoni quickly thawed from liquid N2 temperatures were disrupted by controlled homogenization in isotonic buffered sucrose. Differential centrifugation of the homogenate yielded three particulate and one soluble fractions: the 480 x G pellet (nuclear), the 7650 x G pellet (mitochondrial), the 360,000 x G pellet (microsomal), and the 360,000 x G supernatant (cytosol). Quantitative analysis indicated a major concentration of specific antigenic activities in the microsomal fraction. Further purifications of the urea-solubilized, n-butanol-treated microsomal particles by gel filtration and ionic-exchange chromatography resulted in a microsomal antigen (MAMA) possessing high specific activity and low cross-reactivity. The final purification post-ionic exchange chromatograph showed a 30-fold increase of specific antigen activity over that of the cytosol fraction. Sodium dodecyl sulfate/polyacrylamide gel electrophoresis (SDS-PAGE) and enzyme-linked immunotransfer blot by the "Western blot" technique (EITB) indicated specific antigenic activities in association with several different m.w. bands (heterogeneous m.w. by extrapolation = 4.3 to 11.9 x 10(6), 2.0 x 10(5), and 2.0 x 10(4) daltons) of the MAMA fraction. When compared with other reported serologic antigens, MAMA showed substantially higher specific activity and lower cross-reactivity.

Animals↗
PubMed1979

Effects of high-LET neon (20Ne) particle radiation on the brain, eyes and other head structures of the pocket mouse: a histological study.

A study was made of tissues from 130 pocket mice after a single head-only exposure to high-LET 20Ne particle radiation at 1000, 100 or 10 rad (nominal surface dose) with the view of obtaining base"line data regarding the effectiveness of HZE (cosmic-ray) particles during spaceflight. First seen at 2-3 weeks after exposure, necrotic neurons in the cerebrum reached peak incidence (0 . 04 per cent at 1000 rad, 0 . 003 per cent at 100 rad and less than 0 . 0005 per cent at 10 rad) after 4-5 weeks and decreased to low levels thereafter. Incidence in the cerebellum was lower. Neuroglia, cells of the subependymal matrix and dentate gyrus precursor cells suffered acute damage at 1000 and at 100 rad. At 1000 rad, enlarged hyperchromatic neuroglia, first noted at 3 weeks, increased in number up to 7 months, then declined. Alterations in the retina and olfactory epithelium were seen at 1000 rad, and reaction in the scalp at 100 rad. Damage was incurred by dentinoblasts at 10 rad. Changes similar to those observed in pocket mice were found in the brains of gerbils and C57B1 mice.

Animals↗
PubMedDate not supplied

Somatosensory evoked potentials in lacunar syndromes of pure motor and ataxic hemiparesis.

Syndromes of hemiparetic lacunar infarction include pure motor hemiparesis and ataxic hemiparesis. Twelve such patients were evaluated with somatosensory evoked potentials. Potentials were delayed or absent in all 4 patients with ataxic hemiparesis and in 1 of 8 patients with pure motor hemiparesis. Unlike pure motor hemiparesis, ataxic hemiparesis appears to be associated with abnormal somatosensory evoked potentials. These abnormalities suggest that disturbance of afferent pathways are important in ataxic hemiparesis.

Afferent Pathways↗
PubMedDate not supplied

Quantitative sensory thresholds in carpal tunnel syndrome.

Thresholds for vibration and temperature sensation were quantitated at the second and fifth digits of 23 patients (28 hands) with symptomatic, electromyographically confirmed, carpal tunnel syndrome. Thresholds were determined in 23 age-matched controls. Abnormal sensory threshold data confined to the second digit, apparently related to carpal tunnel syndrome, was found in only 3 (11%) of 28 symptomatic hands. There was no apparent relationship between sensory thresholds and the degree of median nerve abnormality as determined by electromyographic nerve conduction studies. Focal demyelination with minimal axonal loss as is seen in mild or moderate carpal tunnel syndrome appears to be more readily detected by electromyographic nerve conduction studies than by quantitative sensory testing.

Adult↗
PubMedDate not supplied

Reasons patients stay with a dentist.

The intent of the study was twofold. The primary objective was to identify the reasons why patients remain with a practitioner. The secondary objective was to explore a procedure that practitioners could employ to assess the strengths and weaknesses of the way they and their staff interact with patients. The majority of patients indicated that honesty of their dentist was the primary reason that they continued in a dental practice. Patients also cited cleanliness, professional appearance, knowledge of dentistry, gentleness, concern, and quality of work as factors that influenced their decision to stay in a dental practice. The patient survey forms used in this study were designed to be utilized by a private practitioner. The feedback obtained from the survey may be used to improve patient's perceptions and relationship with a dental practitioner.

Adult↗