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M A JESAITIS

Publications and source records attributed to M A JESAITIS.

12 recordsLinked to original sources
PubMed1965

THE INHERITANCE OF THE PROTEIN AND NUCLEIC ACID CHARACTERISTICS OF T2 AND T6 BACTERIOPHAGE.

A study of the immunological properties of phage strains derived from T2 x T6 crosses revealed that the majority of the progeny differ serologically from the parental viruses. Some hybrids were found to contain head membranes having the serological specificity of both T2 and T6 phages, while others contained tail sheaths of the former and the tail fibers of the latter. Since the immunological properties of all hybrids were heritable, it has been concluded that the serological specificity of the head proteins of T2 and T6 is controlled by at least two genetic determinants, and that the specificities of the fiber and sheath proteins may be governed by single genes. Furthermore it was found that nucleic acids of hybrids had similar proportions of unsubstituted, mono- and diglucosylated hydroxymethylcytosine nucleotides to the nucleic acid of either T2 or T6 phage. Since the parental and hybrid viruses having chemically similar nucleic acids contained in some instances serologically different proteins, it has also been concluded that the extent of glucosylation of the hydroxymethylcytosine component of viral nucleic acids and the immunological properties of viral proteins are independently heritable traits of T2 and T6 bacteriophages.

Bacteriophages↗
PubMed1963

Properties of a bacteriophage derived from Escherichia coli K235.

A temperate bacteriophage was isolated from the colicinogenic strain of Escherichia coli K235 and characterized. This phage, termed PK, is related to P2 virus morphologically, serologically, and, possibly, genetically and it bears no relationship to the T-even phages. It was also demonstrated that PK virus and colicine K differ both in their host range and in their immunological specificity, and that PK prophage does not induce the colicinogenesis in its host bacterium. It was concluded that the formation of colicine K. and PK phage in E. coli K235 are controlled by different genetic determinants.

Anti-Bacterial Agents↗
PubMed1961

The inheritance of the glucose component of the phage nucleic acids.

The wild type strains of T2 and T6 bacteriophages differ in their host range specificity, efficiency of plating on E. coli K12, and in glucose content. A study of the inheritance of these three differentiating characteristics has revealed that they are transmitted both upon serial passage of the viruses and when the two phages are crossed. It has been found, furthermore, that an extensive recombination takes place upon crossing. Four types of hybrid phages have been isolated from the progeny of crosses, which had a glucose content of one of the parental phages, and either the host range specificity or efficiency of plating or both of the other. The characteristics of each hybrid were found to be hereditarily stable. It has been concluded that the transmission of the characteristics under consideration is determined genetically and that the genes which control them are not closely linked. Since the glucose content of a phage is determined by the degree of glucosylation of its nucleic acid, the T2 and T6 phages apparently contain genes which control certain chemical properties of their nucleic acid.

Bacteriophages↗
PubMed1957

The nucleic acids of T2, T4, and T6 bacteriophages.

The deoxyribonucleic acids of the wild type strains of the T(2), T(4), and T(6) bacteriophages have been shown to contain glucose as an integral part of the molecule; the amount of hexose present in each nucleic acid differs. A study of the acid degradation products of the three nucleic acids has revealed that in each instance glucose is linked to the apurinic acid component. In the case of the T(6) nucleic acid it was found that two molecules of glucose are linked to hydroxymethylcytidylic acid. The other mononucleotides contained no glucose. From the results which have been presented here, and from data presented by others, it can be concluded that the three viral nucleic acids differ in that they contain different proportions of free and glucose-substituted hydroxymethylcytidylic acids.

Bacteriophages↗
PubMed1955

Lysis of T4 phage by the specific lipocarbohydrate of phase II Shigella sonnei.

When the specific lipocarbohydrate of Phase II Sh. sonnei and T(4) phage react in vitro, the virus is rapidly inactivated and the content of the viral membrane is released into the surrounding medium. The reaction between phage and lipocarbohydrate proceeds only in the presence of a lipide constituent which can be extracted from the polysaccharide, rendering the latter inactive, and which can be replaced by certain fatty acids. It has been suggested that the lipocarbohydrate is the receptor substance of the Phase II bacillus which specifically combines with and brings about disintegration of the virus when the latter infects the host cell.

Bacteriophage T4↗
PubMed1955

Colicine K.

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Anti-Bacterial Agents↗
PubMed1952

The chemical and antiviral properties of the somatic antigen of Phase II Shigella sonnei.

1. The somatic antigen of Phase II Shigella sonnei can be isolated by extracting phenol-killed microorganisms with water. This substance inactivates all T phages to which this bacillus is susceptible. 2. The Phase II antigen is constituted from a protein and a phosphorylated lipocarbohydrate. The major portion of the protein component can be removed by digestion with pancreatin. The enzymatically degraded antigen thus obtained can be further dissociated into its protein and lipocarbohydrate components by treatment with 90 per cent phenol. Glucose, galactose, glucosamine, and an aldoheptose have been identified as the monosaccharide constituents of the lipocarbohydrate.

Antigens↗
PubMed1952

The somatic antigen of a phage-resistant variant of Phase II Shigella sonnei.

1. A phage-resistant variant of Phase II Shigella sonnei has been isolated and the type-specific antigen from it has been obtained. 2. The antigen of the variant microorganism, II/3, 4, 7, differs from that of the parent Phase II bacillus in serological specificity and in chemical make-up. 3. Although the antigen of Phase II Sh. sonnei inhibits all T phages to which the bacillus is susceptible, that of the variant fails to inhibit T(3), T(4), and T(7).

Antigens↗