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Biomedical subjects

M A Gross

Publications and source records attributed to M A Gross.

30 records · Page 2Linked to original sources
PubMed1975

Drug-induced inhibition of myeloid colony growth: protective effect of colony-stimulating factor.

The effect of some potentially myelotoxic drugs on the growth of colony-forming cells in mouse marrow in vitro was examined. Among the drugs tested chloramphenicol, thiamphenicol, and chlorpromazine were inhibitory in therapeutic concentrations, a finding consistent with the common occurrence of a dose-dependent leukopenia from these drugs. The degree of drug-induced inhibition of colony growth was inversely proportional to the concentration of colony-stimulating factor in the medium. It is postulated that the concentration of colony-stimulating factor in the cell milieu in vivo may play a significnat role in determining the occurrence or degree of leukopenia from thses drugs.

Animals↗
PubMed1975

Characteristics of rat carcinoma in culture.

Rat chloroma cells have been propagated in permanent suspension cultures and are grown in Dulbecco's modified Eagle's medium with 10% fetal calf serum and 2.5% horse serum. Several lines have been established; the one of longest duration, Mia C51, has been maintained for over 18 months and has undergone over 100 transfers. Mia C51 cells have a doubling time of 12 hr and maintain many of the properties of the parent tumor, including the characteristic greenish color with high myeloperoxidase activity, an an aneuploid chromosomal pattern, and intact tumorigenicity. They will uniformly produce greenish chloroma tumors when injected into newborn rats. Electron microscopic examination of chloroma tumors and the cultured cells derived from them reveal the presence of extracellular mature and immature type C virus particles morphologically typical of oncornaviruses. Chloroma cells obtained from tumors that lost their alkaline phosphatase activity after repeated transfer regain full activity in culture. Studies using the antigen-antibody crossed electrophoresis indicate that the loss of alkaline phosphatase activity represents a true decrease in alkaline phosphatase protein, which is restored under culture conditions. The availability of a permanent chloroma cell line in culture that maintains the biological properties of the parent tumor provides a useful model for the study of myeloid leukemia.

Alkaline Phosphatase↗
PubMedDate not supplied

Two-stage separation of immature and mature T lymphocytes from myeloerythroid clonogenic bone marrow cells by apheresis and elutriation centrifugation.

Because graft-versus-host disease remains a major complication in allogeneic bone marrow transplantation, a number of techniques capable of removing mature T lymphocytes from bone marrow cells have been attempted. The authors describe a simple two-step procedure using counterflow centrifugation elutriation (CCE) that eliminated 95 to 98 percent of the mature T lymphocytes and greater than 97 percent of the T lymphocyte colony-forming units (CFU-T) while concentrating the bone marrow myeloid colony-forming cells. Viability was greater than 98 percent, and 72 to 98 percent of the total cells separated were recovered. Lymphocyte depletion was substantiated by both morphologic and phenotypic criteria using monoclonal antibodies to T lymphocytes, as well as by responsiveness in mixed-lymphocyte cultures and to mitogens. In addition, this technique separated the lymphoid colony-forming cells from the larger myeloid colony-forming cells. It was concluded that this simple two-step CCE procedure can be used to separate T lymphocytes and CFU-T from myeloid colony-forming cells and offers a means of purging T lymphocytes from large numbers of marrow cells that may be required for human allogeneic bone marrow transplantation.

Aging↗