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Biomedical subjects

M A Gordon

Publications and source records attributed to M A Gordon.

At least 91 records · Page 5Linked to original sources
PubMed1982

Characteristics of tricyclic antidepressant binding sites associated with murine lymphocytes from spleen.

A single population of high affinity, saturable, tricyclic antidepressant (3-H-desipramine) binding sites has been identified on the membranes of murine lymphocytes. There are approximately 300 binding sites per cell and the apparent dissociation constant (Kd) for these binding sites is about 0.4 nM. Competition studies between the radiolabeled ligand in concentrations near the Kd and other members of the class of tricyclic antidepressants indicate that binding is competitive, reversible, and does not display interactions characteristic of cooperativity.

Animals↗
PubMed1982

Tricyclic antidepressant effects on the murine lymphocyte mitogen response.

Tricyclic antidepressant binding sites have recently been detected on the membranes of murine splenic lymphocytes (1). It is reported here that the mitogenic response of murine lymphocytes is altered in the presence of tricyclic antidepressants at concentrations of 10(-5) M or greater. The time-dependent effects of these drugs, when added to the cultures at various times up to 24 hours subsequent to addition of a mitogen (either concanavalin A or lipopolysaccharide B), are also reported.

Animals↗
PubMed1981

Automated immunofluorescence test for toxoplasmosis.

A soluble-antigen fluorescent-antibody technique for serodiagnosis of toxoplasmosis, employing a special fluorometer (FIAX; International Diagnostic Technology, Santa Clara, Calif.), was evaluated in comparison with a standard indirect fluorescent-antibody method which uses a fluorescence microscope. Titers obtained by the new method on 324 serum samples correlated very well with those of the conventional procedure. There was good intralaboratory and interlaboratory agreement in runs on selected samples. The FIAX method is simple, rapid, and reliable and has the advantage of objectivity.

Antibodies↗
PubMed1981

Dermatophilus congolensis infections in wildlife in New York State.

Dermatophilus congolensis infection is described for the first time in a woodchuck (Marmota monax) and a striped skunk (Mephitis mephitis). This actinomycete was also recovered from a raccoon (Procyon lotor) carcass which had been frozen for a year. Encrusted lesions or denuded patches or both were seen during necropsy on the head, thorax, abdomen, and legs of each carcass. D. congolensis was demonstrated in strained smears or paraffin sections or both from the three animals and was recovered from lesions induced in a rabbit's ear by inoculation with infected raccoon tissue. This expands to 59 the number of cases of dermatophilosis found in wildlife and domestic animals in New York State and vicinity.

Actinomycetales↗
PubMed1981

Effects of beta-adrenergic agents on the murine lymphocyte response to mitogen stimulation.

The presence of beta-adrenergic receptors on murine lymphocytes has recently been demonstrated (8). beta-adrenergic agonists and antagonists have been found to alter the murine lymphocyte's response to either Concanavalin A or Lipopolysaccharide B. The regulation of the response to Concanavalin A or Lipopolysaccharide B by these beta-adrenergic ligands is most effective if the beta-adrenergic ligand is added within one hour of the addition of mitogen and at concentrations greater than 1 x 10(-7) M. The possibility that adrenergic modulation of the lymphocyte response may be beta-receptor mediated is supported by the observation of competition between adrenergic agonists and antagonists.

Adrenergic beta-Agonists↗
PubMed1981

Effect of chronic beta-adrenergic therapy on the human lymphocyte response to concanavalin A.

Lymphocytes isolated from cystic fibrosis patients chronically treated with beta-adrenergic agonists respond significantly differently to epinephrine modulation of mitogen challenge than either cystic fibrosis patients not receiving beta-adrenergic therapy or normal human volunteers. Those patients on chronic beta-adrenergic therapy are insensitive to the modulating effects of 1-epinephrine. This observation is discussed in terms of adrenergic receptor alteration and/or different cystic fibrosis disease states.

Adrenergic beta-Agonists↗
PubMed1980

Susceptibility of zoopathogenic fungi to phytoalexins.

Phytoalexins are a group of low-molecular-weight antibiotics produced by higher plants in response to infection by relatively avirulent microorganisms. They are of relatively low toxicity for mammalian cells and have been reported to possess a broad spectrum of antimicrobial activity against bacteria and phytopathogenic fungi. Employing a broth dilution technique, we have found the zoopathogens Petriellidium boydii, Aspergillus flavus, A. fumigatus, Candida albicans, Coccidioides immitis, Cryptococcus neoformans, Histoplasma capsulatum, Rhizopus oryzae, Sporothrix schenckii, and Trichophyton rubrum to be inhibited by one or more of the phytoalexins maackiain, medicarpin, phaseollin, phaseollin isoflavan, pisatin, sativan, and vestitol in concentrations of from 12.5 to 50 microgram/ml. Phaseollin isofalvan was the most effective of these. In agar cup diffusion tests growth of the yeast form of Blastomyces dermatitidis was inhibited by phaseollin at 50 microgram/ml and by phaseollin isoflavan at 25 microgram/ml. Susceptibility of the mycelial form of B. dermatitidis to these two compounds was demonstrated by inhibition of radial extension on agar plates.

Animals↗
PubMed1980

Characteristics of adrenergic binding sites associated with murine lymphocytes isolated from spleen.

Beta-adrenergic binding sites have been identified and characterized on murine lymphocytes. The binding sites are of two distinct classes, a high affinity class which has an apparent dissociation constant of about 1 x 10(-9)M and numbers about 500 sites per cell, and a higher capacity binding class that appears to be more characteristic of a non-specific binding site. The binding of a beta-adrenergic radioligand at concentrations about equal to the Kd (dissociation constant) for the high affinity site can be competed for with classical adrenergic ligands, is reversible and does not display cooperative interactions.

Animals↗
PubMed1980

Association of Malassezia (Pityrosporum) pachydermatis with sarcoptic mange in New York State.

Malassezia (Pityrosporum) pachydermatis consistently accompanied sarcoptic mange in all red foxes (Vulpes fulva), porcupines (Erethizon dorsatum), and coyotes (Canis latrans) examined. This yeastlike microorganism has not heretofore been reported on any of these hosts. Its presence on the exoskeleton of Sarcoptes scabiei taken from these animals suggests a carrier role for the mite. The yeast may be saprophytic or a secondary pathogen.

Animals↗
PubMed1980

Experimental basis of enhancing the immunogenicity of influenza B virus by genetic recombination.

Redistribution of the immunogenicity marker in the course of genetic recombination of influenza B virus was studied in animal experiments on virus strains differing in their ability to induce antibody formation following a single peroral or intraperitoneal immunization. Immunogenicity of influenza virus could be enhanced by recombination of a strain possessing a low activity with a highly immunogenic homotypic strain. Efficiency of the transfer of the immunogenicity marker depended on the properties of viruses used for recombination. Strains at a low passage level proved to be more prospective "donors of immunogenicity" than hyperattenuated thermosensitive viruses which were unsuitable for this purpose. There was no complete correlation between hemagglutinating activities of influenza B viruses and of the recombinant.

Animals↗
PubMed1979

Malassezia pityrosporum pachydermatis (Weidman) Dodge 1935.

Priority of the name Malassezia pachydermatis (Weidman) Dodge 1935 is indicated for the microorganism which has been called Pityrosporum pachydermatis Weidman 1925 and P. canis Gustafson 1955. M. pachydermatis is here further characterized in culture with information drawn from 2 recent isolates, in particular the presence of spiral grooves on the inner surface of the cell wall, good growth on Mycosel agar, rapid production of urease, and assimilation of glucose by the Wickerham method.

Aged↗
PubMed1979

Physicians and collective bargaining.

This article discusses both the history of physician collective bargaining and the applicability of the 1974 health amendments of the National Labor Relations Act to this subject. Gordon outlines the issues for housestaff programs as well as for hospital-based professionals. The scope of physician bargaining includes: wages, hours, working conditions and issues related to the quality of patient care. Housestaff collective bargaining often addresses the issues of on-duty meals, free laundry and uniforms, subsidized housing, parking facilities, and on-call rooms. The concerns of staff physicians include tenure, pensions, and insurance benefits. Gordon suggests that the difficulty and novelty of physician/ hospital collective bargaining will frequently result in an impasse. He states that the propriety or impropriety of a doctors' strike depends upon: (l) the patient-care implications of the grievance causing the strike; (2) the gravity of the grievance leading to work stoppage; (3) the consequences of the strike for patient care; and (4) the other alternatives available. In the view of the author, a doctors' strike may be justified when it arises from a grievance relating to patient care as well as to self-aggrandizement. He states,"...if the strike is the only effective method of making known conditions prejudicial to patient care, including conditions which regularly and systematically cause loss of health and life, the physician may be obliged to engage in such a strike, rather than desist from such action." He concludes that as professionals shift from being private entrepreneurs to salaried employees, they will be compelled to use a collective bargaining approach for professional as well as economic concerns.

Collective Bargaining↗