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M A Gallart

Publications and source records attributed to M A Gallart.

8 recordsLinked to original sources

Dual CD38 and DNA staining of plasma cells in monoclonal gammopathies attained by a rapid, non-wash and automated method.

Dual antigenic and DNA analysis are a prerequisite in order to study DNA content and cell cycle distribution accurately and identify, while studying them separately, neoplastic cells in the mixture of tumour samples with a high proportion of normal cells or when residual normal cells are highly proliferative. We describe a method for the simultaneous detection of surface CD38 antigen and study, by flow cytometry, of DNA content in the bone marrow of patients with a monoclonal gammopathy. This standardized, easy-to-perform, and automated flow cytometric non-wash technique allows dual antigen and DNA staining in less than 30 min. The method identifies bone marrow plasma cells and separately calculates their cell cycle distribution by means of a double staining technique for CD38 surface antigen and DNA content.

ADP-ribosyl Cyclase↗

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