Search PubMed⌕ Search

Biomedical subjects

M A Foley

Publications and source records attributed to M A Foley.

At least 19 recordsLinked to original sources
PubMed2001

Understanding children's activity memory: the role of outcomes.

In three experiments the effectiveness of activity outcomes as memory cues was investigated. In the first experiment, 5-year-olds participated in four activities. In two of these activities, action results were maintained during the unfolding of the activity and perceptually preserved in the activity's end product. In the other two activities, action results "disappeared" from view during the activity and were transformed within the end product. Each activity was recalled under one of four cue conditions: verbal, object, action, and reenactment. For half of the children, the end product was also present during retrieval. Memory for the two types of activities varied with cue condition and with the presence of the end product. In a second experiment, children attempted to describe how the end products could be recreated from the materials used in the activities without participating in them. Patterns of performance confirmed that memory and not inference was responsible for the effects observed in the first experiment. In a third experiment, 5- and 7-year-olds participated in activities of the two types. Within each, picture supports were provided to investigate whether the perceptual availability of action results during encoding influences memory. Results are discussed with respect to an activity memory framework and implications for science education.

Age Factors↗
PubMed2001

A one-bead, one-stock solution approach to chemical genetics: part 1.

BACKGROUND: In chemical genetics, small molecules instead of genetic mutations are used to modulate the functions of proteins rapidly and conditionally, thereby allowing many biological processes to be explored. This approach requires the identification of compounds that regulate pathways and bind to proteins with high specificity. Structurally complex and diverse small molecules can be prepared using diversity-oriented synthesis, and the split-pool strategy allows their spatial segregation on individual polymer beads, but typically in quantities that limit their usefulness. RESULTS: We report full details of the first phase of our platform development, including the synthesis of a high-capacity solid-phase bead/linker system, the development of a reliable library encoding strategy, and the design of compound decoding methods both from macrobeads and stock solutions. This phase was validated by the analysis of an enantioselective, diversity-oriented synthesis resulting in an encoded 4320-member library of structurally complex dihydropyrancarboxamides. CONCLUSIONS: An efficient and accessible approach to split-pool, diversity-oriented synthesis using high-capacity macrobeads as individual microreactors has been developed. Each macrobead contains sufficient compound to generate a stock solution amenable to many biological assays, and reliable library encoding allows for rapid compound structure elucidation post-synthesis. This 'one-bead, one-stock solution' strategy is a central element of a technology platform aimed at advancing chemical genetics.

Combinatorial Chemistry Techniques↗
PubMed2001

A one-bead, one-stock solution approach to chemical genetics: part 2.

BACKGROUND: Chemical genetics provides a systematic means to study biology using small molecules to effect spatial and temporal control over protein function. As complementary approaches, phenotypic and proteomic screens of structurally diverse and complex small molecules may yield not only interesting individual probes of biological function, but also global information about small molecule collections and the interactions of their members with biological systems. RESULTS: We report a general high-throughput method for converting high-capacity beads into arrayed stock solutions amenable to both phenotypic and proteomic assays. Polystyrene beads from diversity-oriented syntheses were arrayed individually into wells. Bound compounds were cleaved, eluted, and resuspended to generate 'mother plates' of stock solutions. The second phase of development of our technology platform includes optimized cleavage and elution conditions, a novel bead arraying method, and robotic distribution of stock solutions of small molecules into 'daughter plates' for direct use in chemical genetic assays. This library formatting strategy enables what we refer to as annotation screening, in which every member of a library is annotated with biological assay data. This phase was validated by arraying and screening 708 members of an encoded 4320-member library of structurally diverse and complex dihydropyrancarboxamides. CONCLUSIONS: Our 'one-bead, multiple-stock solution' library formatting strategy is a central element of a technology platform aimed at advancing chemical genetics. Annotation screening provides a means for biology to inform chemistry, complementary to the way that chemistry can inform biology in conventional ('investigator-initiated') small molecule screens.

Bromodeoxyuridine↗
PubMed1999

Loss of immunoreactivity for human calmodulin-like protein is an early event in breast cancer development.

Cell proliferation requires calmodulin, a protein that regulates calcium-dependent enzymes involved in signal transduction pathways in eukaryotic cells. Calmodulin-like protein (CLP) is found in certain epithelial cell types, including normal breast epithelium, and, although it closely resembles calmodulin in amino acid sequence, CLP interacts with different proteins than does calmodulin. The observation that CLP mRNA expression is dramatically reduced in transformed breast epithelial cells led to two hypotheses: (1) CLP helps to maintain the differentiated state in epithelial cells; and (2) downregulation of CLP accompanies malignant transformation of breast epithelial cells. The objective of this study was to determine if the expression of CLP in human breast cancer specimens is reduced in comparison to its expression in normal breast tissue. Eighty human breast cancer biopsy specimens were analyzed immunohistochemically for CLP expression by using a polyclonal rabbit antihuman CLP antibody. CLP expression was reduced in 79% to 88% of the invasive ductal carcinoma and lobular carcinoma specimens and in a similar fraction of the ductal carcinoma in-situ specimens, compared with normal breast specimens. None of the breast cancer specimens showed an increase in CLP expression. These findings support the hypotheses that CLP behaves as a functional tumor suppressor protein and is downregulated early in breast cancer progression.

Animals↗
PubMed1997

Investigations of closure processes: what source-monitoring judgments suggest about what is "closing".

On many occasions, the perception of an object is based on incomplete information because portions of the object are occluded from view. Nevertheless, people are able to identify an occluded object, suggesting that the identification is mediated by the activation of completion processes which "fill in" the missing information. Although these completion processes are invoked as causal mechanisms for many phenomena, the processes mediating the "completion" have not been specified. The present experiments tested two potential explanations for closure processes. In each experiment, participants were presented with pictures, complete or incomplete in form, and, on a surprise source-monitoring task, they were then asked to remember whether pictures had been presented complete or incomplete in form. Whether viewing pictures of objects, faces, or camouflaged objects, participants showed a pronounced tendency to claim that incomplete pictures were actually presented complete in form. This bias was evident after the participants described functions for objects (Experiments 1, 1A, and 2), after they evaluated properties of faces (Experiment 4), and after they searched for objects hidden in a complex scene (Experiment 5). However, this bias was not evident after they simply named objects portrayed in pictures (Experiment 3). This bias is interpreted as evidence for the activation of implicit imaginal processing. The results of these experiments are informative in their contribution to our understanding of the bases of closure, an intuitively appealing notion frequently invoked to account for object identification, but, as yet, not fully understood.

Analysis of Variance↗
PubMed1990

The effect of exhaled alcohol on the performance of the Datex Capnomac.

We investigated the response of the Datex Capnomac to alcohol in expired air. The greatest response was seen when the Capnomac was set to monitor halothane. There was an approximately linear correlation with blood alcohol levels. This effect is of sufficient magnitude to introduce inaccuracies in the measured concentration of halothane.

Carbon Dioxide↗
PubMed1990

Junior medical staff and the assessment of trauma.

Eighty junior doctors in the acute specialties received a postal questionnaire aimed at assessing their ability to assign priority to the treatment of patients who may have sustained serious trauma. Forty-eight doctors (60%) replied. Of these, three doctors (6%) correctly assigned all patients; 44% failed to identify the three patients in most urgent need of treatment.

Clinical Competence↗
PubMed1989

Discriminating between memories: evidence for children's spontaneous elaborations.

Children are more confused than adults about memories for what they said and what they imagined saying. The present studies examine the extent to which this confusion is related to the person subjects imagine. In Experiment 1, subjects (7, 10, and adult) said words and imagined someone (themselves, a parent, or a friend) saying other words. They were then asked to distinguish words they said from words they imagined. Performance varied with age as well as with the person subjects imagined. Further, performance was better for words subjects imagined than for words they said. Metamemory responses indicated subjects of all ages remembered elaborative processing activated spontaneously during imagination when discriminating between memories. When the nature of subjects encodings was constrained (i.e., subjects said and imagined someone saying words as part of a sentence completion task. Experiment 2), performance declined for all age groups. Experiments 3 and 4 suggest that elaborations reported in response to our metamemory questions occurred during imagination and were not solely prompted by our metamemory questions.

Child↗
PubMed1989

Anagram solving: does effort have an effect?

The purpose of these studies was to explore the role of effort in remembering anagrams and their solutions. In Experiment 1, we compared the effects on memory of copying words, typing them as solutions for easy anagrams, or typing them as solutions for difficult anagrams. Solving anagrams involved more effort than did simply typing words, as indexed by response time. However, this effort facilitated recall for solutions to easy anagrams but not for solutions to difficult anagrams. In Experiment 2, we compared memory for anagrams and their solutions using a frequency-judgment task. Memory for solutions was better than memory for anagrams, and this difference was not affected by anagram difficulty. The results of these studies have implications for our understanding of the role of effort in remembering.

Adult↗
PubMed1988

Phenomenal characteristics of memories for perceived and imagined autobiographical events.

Two studies explored potential bases for reality monitoring (Johnson & Raye, 1981) of naturally occurring autobiographical events. In Study 1, subjects rated phenomenal characteristics of recent and childhood memories. Compared with imagined events, perceived events were given higher ratings on several characteristics, including perceptual information, contextual information, and supporting memories. This was especially true for recent memories. In Study 2, subjects described how they knew autobiographical events had (or had not) happened. For perceived events, subjects were likely to mention perceptual and contextual details of the memory and to refer to other supporting memories. For imagined events, subjects were likely to engage in reasoning based on prior knowledge. The results are consistent with the idea that reality monitoring draws on differences in qualitative characteristics of memories for perceived and imagined events (Johnson & Raye, 1981) and augment findings from more controlled laboratory studies of complex events (Johnson & Suengas, in press; Suengas & Johnson, 1988).

Autobiographies as Topic↗
PubMed1987

Discriminating between action memories: children's use of kinesthetic cues and visible consequences.

The present experiments examined the sorts of cues that might be available to facilitate children's ability to discriminate between memories for their own actions. In Experiment 1, 7 and 10 year olds engaged in two types of tracing exercises (using a pencil and a finger; a stylus and a finger; or a stylus and a pencil). Discrimination performance was better when distinguishing between memories involving the use of a pencil vs. a finger than in either of the other two cases. In Experiment 2, children traced and imagined tracing pictures using one of these three tools. Discriminations between memories for tracing and imagined tracing varied with the type of tool involved and interacted with the type of tracing activity (tracing vs. imagining). These differences in discrimination performance demonstrated the importance of kinesthetic cues and visible consequences for children's memory discriminations.

Attention↗
PubMed1985

Confusions between memories for performed and imagined actions: a developmental comparison.

Children are often assumed to be more confused than adults are about the origin of self-generated memories (e.g., what they did or thought). The present experiments showed evidence in support of this assumption but only under some circumstances. In Experiment 1, 6- and 9-year-olds were as good as adults in distinguishing what they did from what they saw someone else do. However, children had particular trouble distinguishing what they did from what they imagined doing. Confusion between performed and imagined actions was evident across a range of actions. Clustering data also showed that information about origin is part of the memory for an event; all subjects recalled actions according to who performed what action (Experiment 1). Further, the presence of person categories as a basis for organization reduced clustering based on action class more for children than for adults (Experiment 1 vs. 2). Collectively, these findings indicate that children become sensitive to some distinctions in memories sooner than they do to others.

Child↗
PubMed1983

Age-related changes in confusion between memories for thoughts and memories for speech.

The present experiments compared people's abilities to make decisions about the origin of their memories. Experiment 1 demonstrated that 6-year-olds were as good as 17-year-olds in discriminating memories originating from what they said earlier (self-generations) from memories of what another person said earlier (external presentations). However, in both experiments 1 and 2, 6-year-olds were not as good at discriminating what they had said earlier from what they had only thought. The possibility that younger children simply have more difficulty distinguishing between memories originating from the same class, internal or external, was ruled out because 6-year-olds performed as well as 9-year-olds when differentiating between memories from 2 external sources (experiment 2). Nor could their difficulty be attributed to a general problem in distinguishing memories for their thoughts from any other class of memories because they were at no disadvantage in discriminating their earlier thoughts (words they imagined themselves saying) from words someone else said (experiment 2). Our findings suggest that some distinctions, self versus other, emerge as cues in memory sooner than other distinctions, thoughts versus actions.

Adolescent↗