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Biomedical subjects

M A Epstein

Publications and source records attributed to M A Epstein.

At least 163 records · Page 9Linked to original sources
PubMed1974

Observations on the type of infection by Epstein-Barr virus in peripheral lymphoid cells of patients with infectious mononucleosis.

Transformation to continuous cell lines has been studied in cultures of peripheral leuckocytes from infectious mononucleosis (IM) patients and in co-cultures of IM leukocytes and foetal cord blood leukocytes of opposite sex. The transformed cells in the co-cultures were of mixed origin with foetal cells usually predominating. Neutralizing antisera to EB virus markedly reduced or abolished the incidence of transformation in IM leukocyte cultures. This effect was not due to cytotoxicity and followed the pattern seen with cultures where transformation was known to depend on the inter-cellular transfer of infectious EB virus. The findings suggest that EB virus is harboured in peripheral lymphocytes of IM patients as a non-productive unexpressed infection which is activated to produce virus in vitro, the particles released then infecting neighbouring cells to give transformed lines. The differences between this mechanism and the one whereby lines arise in culture from malignant cells of Burkitt's lymphoma are considered, and their significance is discussed.

Blood↗
PubMed1974

Cytogenetic studies on human lymphoblastoid cell lines from Burkitt's lymphomas and other sources.

The occurrence and incidence of a characteristic banding abnormality in a No. 14 chromosome has been studied in Burkitt-lymphoma-derived and certain other EB virus-associated lymphoblastoid cell lines. The abnormality was readily detected in 7 out of 7 Burkitt lines, and when present could be seen in 100% of cells with recognizable No. 14 chromosomes. In contrast, the abnormality was not observed in 775 cells from 31 infectious mononucleosis-derived lines nor in 450 cells from 18 lines obtained from cord blood lymphocytes experimentally transformed by EB virus in vitro. The significance of the abnormality as an indication of cells transformed by the virus in vivo is discussed, and the importance of this considered in relation to a possible oncogenic role for EB virus in man.

Blood↗
PubMed1973

Unusual intranuclear tubular structures associated with the maturation of Herpesvirus saimiri in monkey kidney cell cultures.

Unusual intranuclear tubules have been observed in cultures of both African green monkey and owl monkey kidney cells infected with Herpesvirus saimiri; the material was studied in thin sections with the electron microscope. The tubules were seen in about 10% of virus-containing cells at the stage when the virus matured by budding at the nuclear membrane, measured 160-180 nm in diameter and up to 3.6 μm in length, were bounded by an outer "membrane" and contained beneath this an electron dense repeating structure arranged either as a coil or a series of evenly spaced rings. The morphology and significance of the tubules are discussed.

Animals↗
PubMed1973

The EB virus.

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Antibodies, Viral↗
PubMed1972

Morphological transformation in vitro of human fibroblasts by Epstein-Barr virus: preliminary observations.

Human embryo fibroblasts have undergone morphological transformation in vitro after infection by Epstein-Barr virus. The fibroblasts were maintained in suspension during exposure to the virus, and further treatment with inactivated Sendai virus increased the transformation rate. The transformed cells were large and polygonal and grew in discrete, heaped up, foci.

Cell Transformation, Neoplastic↗