A novel high performance liquid chromatographic (HPLC) procedure has been developed for the quantitation of acetaldehyde in 50 microliter samples of primate whole blood during ethanol metabolism. This microassay has a minimum detectable concentration of about 0.1 microM, displays an intra-assay precision under 10%, and is linear over a reasonable concentration range. Of importance is that negligible acetaldehyde is generated artifactually from ethanol during blood analysis. The assay is based on the reaction of acetaldehyde with 1,3-cyclohexanedione and ammonium ion to form a water-soluble fluorogenic adduct, which is separated by reversed phase HPLC and quantitated fluorometrically. Propionaldehyde, added as an internal standard, forms an analogous separable adduct. Blood acetaldehyde concentrations in Rhesus monkeys were between 1-2 microM 150 min after acute administration of ethanol (1.5 g/kg). Traces of endogenous components which are chromatographically identical with cyclohexanedione adducts of acetaldehyde and formaldehyde also were apparent in blood from monkeys and humans not given ethanol.

Acetaldehyde↗

PubMedDate not supplied

The adsorption kinetics of vaccinia virus: demonstration of reversible adsorption (elution).

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Adsorption↗

PubMedDate not supplied

The relation of work stress, hardiness, and burnout among full-time hospital staff nurses.

In this descriptive study, the author examined the relation of work stress, hardiness, and burnout among 113 full-time hospital staff nurses. It was hypothesized that among hospital staff nurses: 1) the greater personality hardiness, the less job stress; and 2) the greater personality hardiness, the less burnout. Instruments used were Personal Views Survey, Nursing Stress Scale, and Tedium Burnout Scale. Promoting hardiness through nursing staff development programs may help manage stress and reduce burnout in the healthcare setting.

Adaptation, Psychological↗

PubMedDate not supplied

Quality of decision making by registered nurses.

This study investigated quality of decision making in a convenience sample of 26 registered nurses. Instruments used were the Joseph Decision Making Tool, Actual Decision Making instrument, and computer-assisted clinical simulations. Results were correlated with educational preparation, age, experience, practice area, and specialty certification. No differences among basic educational preparation with frequency of actual decision-making practices and willingness to make decisions were found. A positive correlation was found between practice area and frequency of actual decision making (r = 0.639, P < 0.001), and practice area and communication (r = 0.439, P < 0.05).

Adult↗