Biomedical subjects
Publications and source records attributed to M A Calvo.
The fungal density of one hundred and forty-eight samples of sands obtained from forty-two Spanish Mediterranean littoral beaches are investigated. The CFU/g number ranged from 15 and 76 x 10(5). The most commonly isolated genera were: Penicillium, Cladosporium, Aspergillus, Acremonium, Alternaria, Fusarium. The keratinophilic capacity of all the isolated fungal strains was also determined.
We report a new case of a patient with transitional cell carcinoma of the urinary bladder and a solitary metastasis to the orbita. A review of the literature shows two cases described previously. This case is interesting for the clinical features and evolution.
Zinc sulphate (50 mg kg-1 p.o.) did not modify basal gastric mucosal blood flow, as measured by [3H]aniline clearance, but inhibited its reduction by noradrenaline (3.5 micrograms kg-1 min-1). Zinc sulphate also influenced gastric emptying of phenol red but its effects depended upon the dose; 30 mg kg-1 caused no variation whereas 80 mg kg-1 induced a significant delay. The nature of both actions is discussed and their implications in the development and prevention of gastric ulceration have been analysed.
The ability of 17 strains of Epidermophyton to perforate hair in vitro using the Ajello & Georg's test procedure and a modification of Lu's method has been studied. Following the Ajello & Georg's test procedure only E. stockdaleae perforated hair. Sporadically some strains of E. floccosum perforated horse hair. We noted as well unusual perforations originated from inside to outside of the hair. By the other technique, all strains, excepting E. floccosum var. nigricans in child hair, perforated hair. E. floccosum showed these perforations later than E. stockdaleae.
Experimental dermatophytoses were tested in guinea pigs by 17 strains of genus Epidermophyton. Only three strains of E. floccosum produced lesions. These lesions were detected 5-7 d after inoculation, and no crust formation or alopecia was observed. These lesions were also studied using microbiological and histopathological techniques. No infection occurred after cutaneous application of E. floccosum var. nigricans and E. stockdaleae.
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The antifungal activity of 15 mediterranean algae species on some dermatophyte strains (Epidermophyton floccosum, Microsporum canis, M. gypseum and Trichophyton mentagrophytes) and pathogenic yeasts (Candida albicans, C. guillermondii, C. krusei, C. tropicalis and Torulopsis glabrata) has been tested following a modification of Aubert's technique. Among the algae species studied, Falkenbergia rufolanosa is the most active in front of all the fungi tested.
The characteristics of 23 strains of Trichophyton mentagrophytes isolated from clinical samples, were studied. Sabouraud agar was the most adequate medium to inhance at their utmost the different taxonomical features. Variability of enzymatic reactions in strains of T. mentagrophytes was observed.
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The effect of nutrients on the formation and accumulation of secondary metabolites in species of the genus Chrysosporium was examined. The study was performed according to the method of Campbell (1960), and among the results obtained was the fact that the only significant increase in antimicrobial capacity was observed when the strains were grown on a 2% malt extract agar and when micro-organisms were assayed using Aspergillus niger.
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Fifty children with acute diarrhoea, in which the only germ found in stools was Campylobacter fetus jejuni, are studied. Digestive complications occurred are discussed. Nine (18%) had lactose malabsorption, associated in two (4%) with saccharose malabsorption and in one (2%) with cow's milk protein intolerance. All complications were temporary, and carbohydrate malabsorption disappeared between one and three months.
50 Strains of Aspergillus fumigatus were studied for enzymatic and physiologic profile. Proteolytic and lipolytic activity were lacking. Various ribonucleases and sacchrolytic enzymes were found.
Eighty dermatophyte strains belonging to the genera Trichophyton, Microsporum, Epidermophyton and Chrysosporium were screened for their ability to produce extracellular enzymes using a semiquantitative method. The results obtained vary significantly when the studied genera are observed separately, though the enzyme beta-galactosidase was detected in none of the four studied genera.