cDNA library construction for the lambda ZAP-based vectors.
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Bacteriophage lambda↗
Biomedical subjects
M A Alting-Mees
Publications and source records attributed to M A Alting-Mees.
CDNA library construction for the Lambda ZAP-based vectors.
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Bacteriophage lambda↗
Clone excision methods for the Lambda ZAP-based vectors.
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Bacteriophage lambda↗
Polycos vectors: a system for packaging filamentous phage and phagemid vectors using lambda phage packaging extracts.
A new class of hybrid vectors, 'polycos' vectors, incorporate a phage lambda cos site and filamentous phage origin to allow high-efficiency cloning via in vitro lambda packaging extracts. The head-filling mechanism of cos site recognition by lambda packaging proteins permits concatemers of several of these small cos-containing vectors to be packaged per phage particle. Excision of vector monomers after infection is accomplished via a lambda ZAP-like M13 excision process. This system has the advantage of adapting high-efficiency lambda packaging extracts to M13 and phagemid cloning vectors.
Bacteriophage M13↗
pBluescriptII: multifunctional cloning and mapping vectors.
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2-Amino-5-phosphonovalerate↗
pBluescript II: gene mapping vectors.
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Bacteriophage lambda↗