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Biomedical subjects

Lin Li

Publications and source records attributed to Lin Li.

At least 217 records · Page 12Linked to original sources
PubMed2003

[Study of reducing graft-versus-host disease by in vitro blockade of CD40-CD40 ligand co-stimulatory pathway in allogeneic bone marrow transplantation mouse model].

OBJECTIVE: To investigate the effect and its mechanism of reducing graft-versus-host disease (GVHD) by in vitro blockade of CD(40)-CD(40)L pathway in vitro, the donor T lymphocytes cultured in vitro with anti-CD(40)L mAb were transfused in bone marrow transplantation (BMT) GVHD mouse model. METHODS: C57BL/6(H-2b) spleen T cells were isolated as responder cells, and BALB/c(H-2d) spleen cells as stimulator cells. They were cocultured with or without Anti-CD(40)L mAb as anti-CD(40)L mAb group and control group, respectively. At day 5, the mixed lymphocyte response (MLR)-culture cells mixed with bone marrow cells and transfused respectively into the TBI conditioned recipient mice. The mice were divided into two groups: group A, bone marrow cells (2 x 10(6)) and spleen T lymphocytes (2 x 10(6)) from MLR control group; group B, bone marrow cells (2 x 10(6)) and spleen T lymphocytes (2 x 10(6)) from MLR anti-CD(40)L mAb group. The GVHD incidence and hematopoietic reconstitution were observed. Peripheral blood sera and spleen cells of the recipients mice were harvested at scheduled time points for the measurement of cytokines and T cell immunophenotyping with flow cytometry. RESULTS: The incidence of GVHD in group A was 100% (10/10), and in group B was 20% (2/10). The percentage of H-2D(b) positive cells in group B (n = 8) was (93.54 +/- 2.32)% at day 40 after transplantation. The levels of cytokines in serum from group B were significantly lower than those from group A (P < 0.05). The expressions of CD(4)(+), CD(8)(+), CD(4)(+)CD(25)(+), CD(8)(+)CD(25)(+), CD(4)(+)CD(69)(+), CD(8)(+)CD(69)(+) and CD(4)(+)CD(40)L(+) were lower in group B than in group A (P < 0.05). The expressions of CD(8)(+)CD(40)L(+) and CD(4)(+)CD(45)RA(+) were similar in the two groups (P > 0.05). CONCLUSION: Blockade of CD(40)-CD(40)L interaction in vitro could induce immune tolerance in vivo, reduce aGVHD in aGVHD mice model and form chimerism, which was mediated by inhibiting the Th1 and Th2 cytokines production, inducing tolerance of CD(4)(+) and CD(8)(+) cells to alloantigens. The obstruction of T cells activation after tolerance happened mainly at the early and mature phase of T cells activation. These provided the experimental basis for the use of anti-CD(40)L mAb in the clinical transplantation to prevent aGVHD.

Animals↗
PubMed2003

[Clinical studies of phacoemulsification combined with intraocular lens implantation in diabetics].

PURPOSE: To investigate the clinical results of phacoemulsification combined with intraocular lens implantation in diabetics. METHODS: All patients were divided into two groups, which there were 50 cases (61 eyes) with diabetics in the group A and 163 cases (174 eyes) without diabetics in the group B. All cases were treated with phacoemulsification combined with intraocular lens implantation. The level of the fasting blood-glucose in the diabetics must be controlled under 10 mmol/L by the dietotherapy, oral antihyperglycemic or insulin therapy before operation. The follow-up period was 1-28 months, average 8.6 months. RESULTS: The post-operative corrected visual acuities of 0.5 or better were achieved in 47 of 61 (77.1%) eyes in group A and in 140 of 174 (80.5%) eyes in group B, it was statistically no significant difference between the two groups (P > 0.05). The comparison between the two groups showed that there was no significant difference in the intra-operative or post-operative complications(P > 0.05). CONCLUSIONS: As long as the level of the blood surgar was controlled effectively, the treatment of phacoemulsification in diabetics was safe and effective as same as the patients without diabetics. It is very important to control the level of the blood sugar for maintaining the post-operative effect.

Aged↗
PubMed2003

[Construction of the plant expression vectors containing the multiepitope gene of Toxoplasma gondii].

OBJECTIVE: To construct the plant expression vectors containing the multiepitope gene of Toxoplasma gondii (TGMG). METHODS: 1. TGMG was subcloned into pBAC55 vector to construct the intermediate plasmid pB35MG. The E35S/TGMG/NOS3' fragment was cleaved from pB35MG and ligated into the plant binary vector pCAMBIA2300 to construct the plant expression vector pC35MG. 2. Tomato fruit-specific E81. 1 promoter was introduced to pB35MG to construct pB35E1MG vector. The E35SE81. 1/TGMG/NOS3' fragment was subcloned into pCAMBIA2300 to construct the plant expression vector pC35E1MG. 3. Tomato fruit-specific E82.2 promoter was inserted to pB35MG to construct pBE2MG vector. The E82.2/TGMG/NOS3' fragment was subcloned into pCAMBIA2300 to construct the plant expression vector pCE2MG. The insert gene TGMG in the vectors pB35MG, pC35E1MG and pCE2MG were confirmed by sequencing. 4. pC35MG, pC35E1MG and pCE2MG were introduced into Agrobacterium tumefaciens strain LBA4404 competent cell. RESULTS: Digestion with restriction enzymes proved that all recombinant vectors had the inserts with expected length of the target fragments. And the sequencing results were confirmed correct. CONCLUSION: The TGMG intermediate vectors pB35MG, pB35E1MG and pBE2MG and the plant expression vectors pC35MG, pC35E1MG and pCE2MG were constructed successfully, and the three plant expression vectors were introduced into Agrobacterium tumefaciens.

Agrobacterium tumefaciens↗
PubMed2003

Role of calcium mobilization in sodium nitroprusside-induced increase of calcium-activated potassium currents in gastric antral circular myocytes of guinea pig.

AIM: To investigate the role of calcium mobilization in the calcium-activated potassium currents [IK(Ca)] increased by sodium nitroprusside (SNP), a nitric oxide (NO) donor, in gastric antral circular myocytes of the guinea pig. METHODS: A perforated patch-clamp technique was used, and the myocytes were isolated by collagenase. RESULTS: SNP 100 mol/L significantly increased IK(Ca), and enhanced the spontaneous transient outward currents (STOC). SNP-induced increase of IK(Ca) was not blocked by extracellular calcium-free solution (containing egtazic acid 10 micromol/L and nicardipine 5 micromol/L, an L-type calcium channel blocker. And SNP 100 micromol/L suppressed the L-type calcium currents (ICa). SNP-induced increase of STOC was inhibited by heparin 3 g/L, a potent inhibitor of inositol triphosphate receptor (InsP3R). However, ryanodine 10 micromol/L, an inhibitor of calcium-induced calcium release (CICR), did not inhibit the effect of SNP-induced increase of STOC. Methylene blue (1 micromol/L), an inhibitor of soluble guanylate cyclase, also inhibited such an effect. CONCLUSION: The increase of IK(Ca) caused by SNP may be mediated by cGMP via IP3-sensitive calcium pools, however, extracellular Ca2+ may not be involved in the process.

Animals↗
PubMed2003

[Structure and function of several intercropping ecosystems in newly built orchard].

Sloping wildland is the important potential land resource in China. Structures and functions of five optimized models selected from more than 10 intercropping patterns in the newly built orchards in the uncultivated sloping field were analyzed in this paper. The results showed that organic matter was increased by 5-20%, total N by 7-40%, total P by 8-70%, and total K by 15-80% yearly. Considering its benefit in soil and water conservation, the intercropping model of peanut and autumn soybean was the best, and followed by spring soybean and autumn soybean, tomato and Chinese cabbage, cayenne and tomato, and drought rice and red turnip. Compared with the control, the average soil erosion module and runoff amount of the 5 models were decreased by 44.19%, 38.24%, 39.52%, 37.56%, 37.30%, and 22.40%, 9.28%, 24.11%, 21.60%, 15.93%, respectively. The intercropping model of cayenne and tomato had the highest biomass, and its annual biomass averaged 1,00,267 kg.hm-2. The biomass of intercropping model of peanut and autumn soybean was the second, and the annual value averaged 77,026 kg.hm-2. Intercropping model of peanut and autumn had the highest annual productivity, which averaged 15,619 kg.hm-2, and that of intercropping model of tomato and Chinese cabbage was the second, averaged 15,394 kg.hm-2. The efficiency of energy introjection was in order of cayenne and tomato, tomato and Chinese cabbage, peanut and autumn soybean, spring soybean and autumn soybean, and dry rice and red turnip, and their values were 3.06, 2.16, 2.15, 2.06, and 0.71, respectively.

Crops, Agricultural↗
PubMed2003

[Characterization of phenylpyruvic acid and beta-benzyl phenylpyruvic acid by UV, IR, MS, and 1H NMR].

Characterization of phenylpyruvic acid (PPA) and beta-benzyl phenylpyruvic acid (BPPA) by UV, IR, MS and 1H NMR has been carried out. It is found that the absorption at 240 and 365 nm are observed in the UV spectrum of PPA and the absorption at 210 nm is the only one in BPPA. These demonstrate that PPA has the olefinic alcohol structure and BPPA hasn't. The IR absorption at 1,624.38 and 1,697.25 cm-1 of PPA are originated from two carboxy C=O because of its gem-diol structure, and the absorption at 1,706.6 and 1,728.6 cm-1 in BPPA is assignable to keto C=O and carboxy C=O, respectively. The main peaks in the MS spectrum of PPA are 164, 119, 91, 65, 45, 39, and BPPA 254, 236, 209, 181, 91, 77, 65, 39. The peaks at 4.124, 6.381, 7.184-7.748, 9.268 are observed in the 1H NMR spectrum of PPA and the peaks in BPPA are 2.90-3.02, 3.29-3.40, 4.73-4.81, 7.04-7.44, 8.99.

Benzyl Compounds↗
PubMed2003

[Effects of compound yi-zhi on learning and memory disorder and beta-amyloid protein induced neurotoxicity].

OBJECTIVE: To investigate the effects of Compound Yi-Zhi (CYZ) on learning and memory disorder and beta-amyloid protein induced neurotoxicity. METHODS: Various doses of CYZ were administered to SD rats for 8 days, twice a day. Then scopolamine hydrobrominde intraperitoneal injection was performed on each rat, and the MORRIS water maze test and step-though test were carried out respectively to explore the changes of the rats' learning and memory capacities. Primary rat cortex neurons were cultured in vitro for 7 days, and then serum containing CYZ was added to neurons before or after the addition of beta Amyloid peptide25-35 (A beta 25-35) to observe CYZ's protectiveness on neurotoxicity. MTT assay and test of level of LDH in the culture media were performed to achieve this aim. RESULTS: Compared with control group, in MORRIS water maze test time of finding the platform under the water surface decreased, and in step-though test the latent period rose and the error number got smaller. Moreover, in cultured primary neurons, the dramatic drop of LDH level in culture media and the high OD scores in MTT test indicated that CYZ could effectively resist the neurotoxicity induced by A beta 25-35. CONCLUSION: CYZ presented promising effects on learning and memory dysfunction and A beta-induced neurotoxicity in vitro.

Amyloid beta-Peptides↗
PubMed2003

[Study on factors associated with heterosexual-transmission of human immunodeficiency virus in central China].

OBJECTIVE: To investigate the factors of the human immunodeficiency virus (HIV) associated with sexual-transmission in central China. METHODS: (1) Cross-sectional study: couples that one was HIV positive were selected in Henan and Hebei province of China. The couples must be 20 - 50 years old with normal function on sexual intercourse. Cordant couples that subsequently infected partners were at risk of infection solely through sexual contact with the HIV-seropositive partner and the discordant couples that the seronegative partners were at risk of infection solely through sexual contact with the HIV-seropositive partner, were selected. Plasma viral load, CD4 cell count were tested. (2) Case-control study was used to compare 7 sexual transmitted cases and 56 nontransmitted controls with respect to the frequency of sexual intercourse, plasma viral load and CD4 cell count. RESULTS: (1) A total of 87 couples that at least one partner was HIV positive were recruited include 56 discordant couples and 7 cordant couples with whom sexual transmission had happened. The rate of sexual transmission was 11.1% among those at the risk of sexual transmission. (2) Of the discordant couples, male positive rate 25%, female positive was 75%. (3) The risk for transmission was higher in those couples with the frequency of unprotected vaginal sexual intercourse (> or = 4 times per month) than the reference group (< 4 times per month) (Fisher's exact test, P = 0.047, OR = 8.0). Median plasma viral load was significantly higher in the antecedent infected partners of cordant couples than the positive partner of discordant couples (378,285.71 vs 136,578.57 copies/ml, t = 3.591, P < 0.01). The odds ratio was 22.0 for plasma viral load > or = 100,000 copies/ml compared with the reference group of < 100 000 copies/ml (Fisher's exact test, P = 0.016). The CD4 cell count and CD4/CD8 of the transmitted group were significantly lower than that of the nontransmitted (t = 2.767, P < 0.05; t = 6.06, P < 0.05). CONCLUSIONS: The frequencies of heterosexual-transmission in central China were relatively low. The risk of heterosexual transmission was related to the frequency of sexual intercourse. Higher plasma viral load and lower CD4 count was strongly correlated with high risk of heterosexual transmission.

Adult↗
PubMed2003

[Genetic analysis of a rolled-leaf mutant in rice population of T-DNA insertion].

A rolled-leaf mutant was obtained in a T-DNA(containing bar gene and Ds element) insertion population, which consist of transgenic japonica rice Zhonghua 11 mediated by Agrobacterium tumefaciens. Through self-hybridization of three generations, one of trait-purified mutants (R1-A2) was obtained and used as parent to cross with variety Zhonghua 11. The leaves of 36 F1 plants investigated were rolled and resistant to herbicide Basta. Among 852 F2 plants, the segregation ratio of rolled leaves to normal leaves(645:207) was consistent with 3:1. All rolled-leaf plants were resistant to herbicide Basta, and all normal leaf plants were sensitive to herbicide Basta. These results showed that the trait of rolled-leaf is co-segregated with Basta resistance. The total DNA of 45 rolled-leaf plants and 30 normal leaf plants in F2 population were amplified to test the presence of T-DNA by Ds primers. The results showed that the positive band were amplified in all rolled-leaf plants, but not in every normal leaf plant. In F1B1 progenies, all plants which derived from backcross parent R1-A2 were rolled leaves; while variety Zhonghua 11 was used as backcross parent, the segregation ratio of rolled-leaf to normal leaf was consistent with 1:1. Taking these data together, it indicated that the rolled-leaf mutant was co-segregation with T-DNA and controlled by single dominant gene.

Agrobacterium tumefaciens↗
PubMed2003

[Early repair of full layer eyelid defect caused by chemical burn].

OBJECTIVE: To investigate the optimal time and method of the early repair of the full layer eyelid defect caused by chemical burn. METHODS: Free nasal septum mucosal cartilage flap with muscle flap, skin grafting, or skin flap were performed in 18 cases (19 eyelids) with chemical burn within 4 postburn weeks. Eyelid reconstruction and corneal transplantation were performed at the same time in 4 patients. RESULTS: All the reconstructed eyelids and transplanted cornea survived. The incidence of severe complications, such as exposure keratitis, corneal ulcer and eyeball perforation decreased. CONCLUSION: Full layer eyelid defect caused by chemical burn should receive early reconstruction and repair, including timely reconstruction of eyelid for the sake of protecting the eyesight and of alleviating the inflammatory reactions, and the corneal transplantation should be done at the same time to avoid corneal perforation. Nasal septum mucosal cartilage flap could be ideal for the eyelid reconstruction.

Adult↗
PubMed2003

Chinese mothers' knowledge and attitudes about breastfeeding in Perth, Western Australia.

The objective of the research was to describe Chinese-Australian (Mandarin speaking) mothers knowledge about and attitudes towards breastfeeding. Data for this cross-sectional survey was obtained by telephone interviews conducted in Mandarin. A sample of 506 Mandarin-speaking women was recruited and interviewed in Perth, Western Australia. The majority came from mainland China (81.6%), were aged between 23 and 59 years, and had some tertiary education (76.3%). Most of the mothers (90.9%) indicated that they supported breastfeeding for all infants and most had some knowledge about the benefits of breastfeeding. The main reasons that mothers considered stopping breastfeeding were not having enough breastmilk and going back to work or study. The higher the family income, the less preference toward breastfeeding. These findings highlighted the significance of social and cultural factors that impact on the women's decisions to initiate and maintain breastfeeding.

Adult↗
PubMed2003

[Eletrocatalytic oxidation for organic pollutant on the nanoparticle platinum coated titanium electrode].

The nanoparticle platinum coated titanium electrode was prepared using the method of electrochemical cathodic reduction followed by anodic oxidation. The surface microstructure of electrode has demonstrated that platinum particles dispersed in the three-dimensional network of channels of the titanium oxide film were in the supper-fine and well-distributed state. The nanoparticle platinum electrode owned plenty of active points and well electrocatalytic property based on the exposed platinum particles with very small diameters. Electrochemical behaviors of two kinds of platinum electrode for methanol has been performed through Cyclic Voltammetry. The results have showed that the nanoparticle platinum electrode had a much better electrocatalytic property for methanol than smooth platinum electrode in acidic, neutral or alkaline medium. The oxidation current densities on nanoparticle platinum electrode are over 100 times of that on smooth platinum electrode. Furthermore, the values of average current efficiency on nanoparticle platinum electrode are several times of that on smooth platinum electrode when degrading three kinds of organics methanol, phenol and methyl orange. This clearly revealed that the nanoparticle platinum electrode had stronger ability to degrade organic pollutants.

Catalysis↗
PubMed2003

[The effects of the tangfukang capsules on cytokines of early diabetic nephropathy].

OBJECTIVE: To find out the mechanism of Tangfukang on the diabetic nephropathy(DN). METHOD: A Model of streptorotocin diabetic rats was used. The expressions of the AGEs, ICAM-1, IV-C and FN were observed in renal cortex of diabetic rats with immunohistochemical method. The level of the IL-1 beta in blood serum was measured by radioimmunoassay method and the level of TNF was determined in blood serum by ELISA method. Aminoguanidine was selected as the control medicine which could inhibit the expression of monenzymatic glycosylation end products of protein. All the results were compared to analyze the effect of Tangfukang on monenzymatic glycosylation of protein and cytokine expression in early diabetic nephropathy rats. RESULTS: Both Tangfukang and aminoguanidine significantly decreased the expression of AGEs, but Tangfukang was greatly superior to aminoguanidine in suppressing the expression of cytokine like ICAM-1, IL-1 beta, TNF-alpha and the ingredients of extracellular matrix like IV-C, FN. CONCLUSION: It may be the partical mechanism of Tangfukang to suppress the monenzymatic glycosylation of protein and to reduce the expression of cytokine in diabetes. The effect of Tangfukang is superior to that of aminoguanidine.

Adrenal Cortex↗
PubMed2003

[Polymorphism analysis of porcine myogenin gene by PCR-RFLP].

The polymorphisms of porcine myogenin gene in 561 pigs including Duroc, Landrace, Large Yorkshire, Nanchang white pig, Erhualian, Meishan, Yushan black pig, Leping spotted pig, Jinhua black head-hind pig and Shanggao black head-hind pig were detected by PCR-RFLPs with three different pairs of primers,and the PCR products were digested by MspI. The results showed that most of the Duroc, Landrace, Large Yorkshire, Nanchang white pigs presented as AA genotype, while more animals of the six Chinese local pig breeds except for Leping spotted pig presented as BB genotype at PCR1 MspI-RFLP site. The six Chinese local breeds presented as MM genotype except that one Yushan black pig presented as MN genotype, while more swines of the exotic breeds including Duroc, Landrace, Large Yorkshire presented as NN genotype, and Nanchang white pig appeared to be closer to the exotic breeds at PCR2 MspI-RFLP site. PCR product was obtained in all the swine by PCR3, but the MspI restriction site was not found in the tested pig breeds including Meishan and blood closely related Erhualian pig.

English Abstract↗
PubMed2002

Studies with GIP/Ins cells indicate secretion by gut K cells is KATP channel independent.

K cells are a subpopulation of enteroendocrine cells that secrete glucose-dependent insulinotropic polypeptide (GIP), a hormone that promotes glucose homeostasis and obesity. Therefore, it is important to understand how GIP secretion is regulated. GIP-producing (GIP/Ins) cell lines secreted hormones in response to many GIP secretagogues except glucose. In contrast, glyceraldehyde and methyl pyruvate stimulated hormone release. Measurements of intracellular glucose 6-phosphate, fructose 1,6-bisphosphate, and pyruvate levels, as well as glycolytic flux, in glucose-stimulated GIP/Ins cells indicated that glycolysis was not impaired. Analogous results were obtained using glucose-responsive MIN6 insulinoma cells. Citrate levels increased similarly in glucose-treated MIN6 and GIP/Ins cells. Thus pyruvate entered the tricarboxylic acid cycle. Glucose and methyl pyruvate stimulated 1.4- and 1.6-fold increases, respectively, in the ATP-to-ADP ratio in GIP/Ins cells. Glyceraldehyde profoundly reduced, rather than increased, ATP/ADP. Thus nutrient-regulated secretion is independent of the ATP-dependent potassium (K(ATP)) channel. Antibody staining of mouse intestine demonstrated that enteroendocrine cells producing GIP, glucagon-like peptide-1, CCK, or somatostatin do not express detectable levels of inwardly rectifying potassium (Kir) 6.1 or Kir 6.2, indicating that release of these hormones in vivo may also be K(ATP) channel independent. Conversely, nearly all cells expressing chromogranin A or substance P and approximately 50% of the cells expressing secretin or serotonin exhibited Kir 6.2 staining. Compounds that activate calcium mobilization were potent secretagogues for GIP/Ins cells. Secretion was only partially inhibited by verapamil, suggesting that calcium mobilization from intracellular and extracellular sources, independent from K(ATP) channels, regulates secretion from some, but not all, subpopulations of enteroendocrine cells.

Adenosine Diphosphate↗
PubMed2002

Potent suppression of viral infectivity by the peptides that inhibit multimerization of human immunodeficiency virus type 1 (HIV-1) Vif proteins.

Virion infectivity factor (Vif) is essential for the replication of human immunodeficiency virus type 1 (HIV-1) in vivo, but its function remains uncertain. Recently, we have shown that Vif proteins are able to form multimers, including dimers, trimers, or tetramers. Because the multimerization of Vif proteins is required for Vif function in the viral life cycle, we propose that it could be a novel target for anti-HIV-1 therapeutics. Through a phage peptide display method, we have identified a set of 12-mer peptides containing a PXP motif that binds to HIV-1 Vif protein. These proline-enriched peptides potently inhibited the Vif-Vif interaction in vitro. We have also screened a set of synthesized Vif peptides (15-mer), which covers all the amino acids of the HIV-1 Vif protein sequence, for their ability to inhibit the Vif-Vif interaction in vitro. We demonstrated that Vif-derived proline-enriched peptides that contain the (161)PPLP(164) domain are able to inhibit the Vif-Vif interaction. Conversely, the deletion of the (161)PPLP(164) domain of Vif protein will significantly impair the capability of Vif proteins to interact with each other, indicating that the (161)PPLP(164) domain plays a key role in Vif multimerization. All these results demonstrate that the proline-enriched peptides block the multimerization of Vif through interfering with the polyproline interfaces of Vif formed by (161)PPLP(164) domain. Moreover, these peptides which inhibit the Vif-Vif interaction in vitro potently inhibit HIV-1 replication in the "nonpermissive" T-cells. We propose that this study starts a novel strategy to develop structural diverse inhibitors of Vif such as peptidomimetics or small organic molecules.

Amino Acid Sequence↗
PubMed2002

[Inhibition of activation of nuclear factor-KappaBeta enhances apoptosis induced by chemotherapeutic drugs in P388 cell line].

OBJECTIVE: To explore the effects of glucocorticoid on apoptosis of mouse acute lymphocytic leukemia P388 cells and activation of nuclear factor-Kappa-gene binding (NF-KappaBeta) in P388 cells induced by chemotherapeutic drugs. METHODS: Mouse acute lymphcytic leukemia P388 cells were cultured. with 1.0 micro mol/L thasone (DXM) hours. Then Cytosine arabinoside Ara-C), cyclophosphamide Cy , and etoposide VP16 , of different concentrations were added into the cultures. Twelve hours later, the apoptosis induced by these drugs in P388 cells was analysed by TdT-mediated kappa-dUTP nick and end labeling (Tunel) and DNA electrophoresis. P388 cells in the exponential growth stage (1 x 10(6)/ml) were cultured with DXM (1.0 micro mol/L) for 2 hours. Then Ara-C, Cy, DXM, and VP16 were added into the culture. Three hours later electrophoretic mobility shift assay (EMSA) was conducted to determine the DNA binding activation of NF-KappaBeta. RESULTS: The highest apoptosis rates of P388 cells induced by Ara-C, Cy, and VP-16, were 48.3% +/- 2.6%, 21.4% +/- 6.8%, and 38.1% +/- 3.8%, respectively. The apoptosis rate of P388 cells induced by Ara-C + DXM was 69.6% +/- 7.7%, 44% higher than that induced by only Ara-C (48.3% +/- 2.6%); the apoptosis rate of P388 cells induced by Cy + DXM was 35.6% +/- 6.8%, 89% higher than that induced by only Cy (21.4% +/- 6.8%); and the apoptosis rate of P388 cells induced by VP-16 + DXM was 71.9% +/- 9.9%, 66% higher than that induced by only VP-16 (38.1% +/- 3.8%) (all P < 0.01). Ara-C, Cy, and VP-16 significantly increased the activation of NF-KappaBeta in P388 cells. Pre-processing of 1.0 micro mol/L DXM suppressed the activation of NF-KappaBeta in P388 cells induced by Ara-C, Cy and VP16 by 90 , 71 and 68 respectively. CONCLUSION: Chemotherapeutic drugs (Ara-C, Cy, and VP-16) induce apoptosis and meanwhile induce the activation of NF-KappaBeta in P388 cells. Glucocorticoids, such as DXM, suppress the activation of NF-KappaBeta in leukemic cells, thus increasing the apoptosis induced by chemotherapeutic drugs.

Animals↗