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Biomedical subjects

Li Zhang

Publications and source records attributed to Li Zhang.

At least 613 records · Page 34Linked to original sources
PubMed2003

A novel polymorphism A(+884)-->G in the hepatic lipase gene and its association with coronary artery disease.

Hepatic lipase (HL) activity may influence susceptibility to coronary artery disease (CAD). Association between the single nucleotide polymorphisms (SNPs) in the HL gene with the occurrence of CAD has been investigated thoroughly, but to date most studies focused on the base variation in the promoter of HL gene, little is known about the variation in the coding region. In present study, the SNP in all exons of the HL gene were analyzed. All 9 exons with their flanking sequences of the HL gene were amplified from the Chinese patients with CAD and normal controls by PCR technique, and the PCR products were detected by denaturing high performance liquid chromatography (DHPLC) and sequenced with a dideoxy terminal termination method. As the result, a novel SNP A(+884)-->G within the sixth exon of HL gene was found, the 276 codon AAA was changed into AGA and resulted in the substitution of arginine for lysine. Compared with the control group, more CAD patients carried the G+884 allele (AG+GG) (54.9% vs. 41.5%, chi(2)=6.164, df=2, P=0.046). The prevalence of the G+884 allele was significantly higher in the CAD patients than that in control subjects (31.4% vs. 21.3%, chi(2) =4.652, df=1, P=0.031). Data from the linkage disequilibrium analysis showed that the A(+884)-->G polymorphism was strong in linkage disequilibrium with the T(-2)-->C variation we identified previously(D'=0.699, 0.742 in CAD patients and controls, respectively), and the frequency of the C(-2)/G(+884) haplotype (mutation) is significantly higher in CAD patients than that in controls (0.253 vs. 0.172, P<0.05).

Coronary Disease↗
PubMed2003

[Cloning and characterization of mitochondrial NADH dehydrogenase gene of Cysticercus cellulosae].

OBJECTIVE: To clone and characterize the NADH1 gene of Cysticercus cellulosae. METHODS: A cDNA library was constructed from Cysticercus cellulosae and was immunoscreened by using rabbit anti-Cysticercus cellulosae polyclonal antibody. The gene structure and its possible function were analyzed by comparing with sequences available in the GenBank, after the insert of positive clone was subcloned and the nucleotide sequence of the insert was determined by dideoxynucleotide chain termination method. RESULTS AND CONCLUSION: A cDNA clone (named TS5) with a length of 1,082 bp was isolated. The 5' terminal of cloned gene contained one open reading frame of 1-578 bp encoding 192 amino acid residues of mitochondrial NADH dehydrogenase subunit 1 and the 3' terminal contained three kinds of tRNA genes.

Amino Acid Sequence↗
PubMed2003

[Clinical characteristics of non-steroidal anti-inflammatory drugs associated gastroduodenal ulcer bleeding].

OBJECTIVE: To study the clinical characteristics of non-steroidal anti-inflammatory drugs (NSAIDs) associated gastroduodenal ulcer bleeding. METHODS: Patients who were hospitalized in our ward because of gastroduodenal bleeding from Jan, 1997 to Dec, 2001 were divided into two groups according to consumption of NSAIDs or not in the week previous to the onset of bleeding. RESULTS: 446 patients were investigated with 86 in NSAIDs group and 360 in non-NSAIDs group. There was no significant difference in sex, way of bleeding, history of peptic ulcer, bleeding site in stomach or duodenum, presence of erosion and the need of endoscopic injection therapy or not between the two groups. However, the patients in NSAIDs group were older than those in non-NSAIDs group. Hemoglobin level was lower in NSAIDs group (P = 0.004). There was more gastric ulcer and complex ulcer in the NSAIDs group than in non-NSAIDs group (P < 0.001). NSAIDs users had more ulcers (P < 0.001). Helicobacter pylori (Hp) was present in 72.5% of the non-NSAIDs group and 53.4% of the NSAIDs group (P = 0.001). In an another study, we found that age of the patients or Hp state didn't affect the clinical characteristics of NSAIDs ulcer bleeding. CONCLUSIONS: The clinical characteristics of NSAIDs associated gastroduodenal bleeding should be better understood so as to decrease the occurrence of NSAIDs ulcer and its complication.

Adolescent↗
PubMed2003

[Causes of ineffectiveness of microvascular decompression for hemifacial spasm].

OBJECTIVE: To study causes of ineffectiveness of microvascular decompression (MVD) in treatment of hemifacial spasm (HFS). METHODS: Reoperative MVD was performed in 23 HFS patients with previous ineffective MVD. In the patients, the main causes of ineffectiveness included misjudgment of compressing vessels (7 patients), improper insertion of decompressing grafts (9), improper selection of grafts (5) and small grafts (2). RESULTS: Symptoms of HFS disappeared immediately after the second MVD in 21 patients and delayed in 2 patients (after 2 weeks, 6 weeks). No recurrence of HFS was noted during the follow-up period of 1.0 - 6.0 years (mean 3.4 years). CONCLUSIONS: MVD is an effective microsurgical method for treating HFS. Accurate judgement of compressing vessels and proper decompression are the key to surgical effectiveness.

Adult↗
PubMed2003

[Human leucocyte antigen-DPA1 polymorphism distribution in Chengdu Chinese families with intrahepatic cholestasis of pregnancy].

OBJECTIVE: To investigate the relationship between human leucocyte antigen (HLA)-DPA1 region gene polymorphism and intrahepatic cholestasis of pregnancy (ICP). METHODS: The HLA-DPA1 alleles of 25 ICP families and 25 normal control group families were analysed by polymerase chain reaction-sequence specific primers(PCR-SSP) method. RESULTS: The analysis of the frequencies and feto-maternal or couples sharing of the DPA1 alleles showed that no statistically significant difference was observed between the two study groups. CONCLUSION: It is unlikely that an HLA-DPA1 allele is primarily associated with susceptibility or resistance to ICP.

Alleles↗
PubMed2003

[Vegetation state and soil enzyme activities of copper tailing yard on Tongguan mountain].

From the open investigation and laboratory analysis, this paper studied the vegetation state and soil enzyme activities of copper tailing yard of Tongguan mountain. The results showed that there were 34 species of natural colonized plants on copper tailing yard, subordinated to 16 families and 33 genera, and regard herbs as principle, and many for 1-2 years old. The main families were compositae (10 species), gramineae (9 species) and legumineceae (2 species). Hippochaete ramosissimum, which belonged to equisetaceae, had and significant dominant. There were some microcoenses such as Hippochaete ramosissimum + Imperata cylindraca community, Cynodon dactylon + Imperata cylindraca community and Phragmites australis community. But, the vegetation on copper tailing yard was distributed in spot piece and scattered mainly with single species. The activities of three soil enzymes had a stronger sensitivity to the vegetation state, and their relativity to the vegetation state was in order of urease > sucrase > catalase. It's suggested that unrease activity could be used as an indicator index for the reclamation of wasteland.

Catalase↗
PubMed2003

[Analysis of subtype of porcine endogenous retrovirus in two species of Chinese pigs].

OBJECTIVE: To analyze the subtype of porcine endogenous retrovirus (PERV) in two species of Chinese pigs-Wu zhishan pig and Banna minipig inbred; the total number of pigs being eighty six. METHODS: Three primers which are specific for the three PERV subtypes (subtypes A, B and C) were used to amplify the genome DNA extracted from the pigs. RESULTS: In all tested pigs' genome, subtype C was not found; in 77.9% pigs, double positive envAB were found; in 22.1% pigs, only A or B was found. CONCLUSION: In the genome of peripheral leukocytes from Wuzhishan pig and Banna minipig inbred, only subtypes A and B exist, and envAB predominate.

Animals↗
PubMed2003

[Multivariate survival analysis for 423 patients with non-small cell lung cancer].

BACKGROUND & OBJECTIVE: Non-small cell lung cancer(NSCLC) is characterized by high incidence and poor prognosis in our country. New prognostic factors are needed to guide the treatment of patients with NSCLC. This study was designed to evaluate the characteristics and outcome in NSCLC patients who underwent completely surgical resection. METHODS: A total of 423 cases were analyzed using SPSS10.0 software. The survival differences were compared by univariate and multivariate analysis. RESULTS: The 5-year survival rate in this group was 46.98%. Multivariate analysis identified two poor prognostic factors: advanced TNM stage (P=0.003) and blood group B (P=0.02). Multivariate analysis also identified two better prognostic factors:adjuvant chemotherapy (P=0.04) and squamous histological type (P=0.005). The number of the risk factors (blood group B and non- squamous) were negatively related to prognosis according to multivariate analysis in stage III a NSCLC (the 5-year survival rates:43.5% vs 23.7% vs 4.0%, P< 0.001). CONCLUSION: Adjuvant chemotherapy maybe is beneficial to NSCLC patients who underwent completely surgical resection. Cox multivariate analysis demonstrates that evaluation of both histological type and blood-group has a significant prognostic effect in stage IIIa patients.

ABO Blood-Group System↗
PubMed2003

P-glycoprotein restricted transport of nimodipine across blood-brain barrier.

AIM: To examine whether the transport of nimodipine (NMD) from the circulating blood into the brain is restricted by P-glycoprotein (P-gp) in rat brain capillary endothelial cells (BCEC). METHODS: When cells reached confluence, a time course of NMD uptake was recorded by incubation with a medium containing NMD 10 mg/L at 37 degrees C. Effects of various agents in the steady-state uptake of NMD were tested by co-administration with NMD and each compound to cells at 37 degrees C for 90 min. The uptake of NMD was measured for 90 min. Effects of P-gp inhibitors on the efflux of NMD from primary cultured BCEC were studied by administration of erythromycin, clarithromycin, cyclosporin A (CsA), and Hanks' solution after the accumulation of NMD by BCEC at 37 degrees C for 90 min. RESULTS: The uptake of NMD by primary cultured rat BCEC was time-dependent, and the steady-state uptake of NMD was increased in the presence of several substrates of P-gp in BCEC. The steady-state uptake was also significantly increased (P<0.01) when cellular ATP was depleted by treatment with sodium azide. Furthermore, efflux of NMD from BCEC was inhibited by erythromycin,clarithromycin, and CsA. CONCLUSION: The permeability of NMD into the brain is restricted by P-gp and increased by co-administration with P-gp inhibitors.

ATP Binding Cassette Transporter, Subfamily B, Mem↗
PubMed2003

[Distraction osteogenesis for correction of maxillo-mandibular deformity].

OBJECTIVE: To study the application of extraoral distraction devices in correction of various maxillo-mandibular deformities. METHODS: From 1997, 11 cases with various maxillo-mandibular deformities were corrected by distraction devices of designed by myself. 7 cases of then were the patients with cleft palate who have severe secondary maxillary hypoplasia. 1 case with micrognathia, 1 case with partial mandibular mentum defect, 1 case with lateral deviation of micrognathia and 1 case with hemimandibular hypertrophy. The mandibles were elongated, from 12 mm to 20 mm, the maxillae were elongated from 7 mm to 22 mm, the vertical mandibular distraction was 15 mm. RESULTS: All cases were corrected satisfactorily and haven't any complications. In 1 cases the 1 Kischners pin had been pull out by loosen during distration. 4 Kischners pin were curved during vertical mandibular distration. Another case the maxillary hypoplasia appeared edge-to-edge and mild open occlusion after 4 weeks fixation. CONCLUSION: The extraoral distration osteogenesis provides a new method with many advantages for those maxillo-mandibular deformities that are difficulty corrected by ordinary orthognathic surgery.

Cleft Palate↗
PubMed2003

[An epidemiologic study for relation between the snore and cerebral infarction].

OBJECTIVE: To explore the relation between cerebral infarction and simple, apneic snore. METHOD: A case control comparative study with the exception of influencing factors such as ages, sex, obesity and so on in 60 patients with cerebral infarction and 60 control subjects. RESULT: The probability of cerebral infarction is a little high in all snorer compared with the no snorer. The Odds ratio is 1.709 (95% confidence limits 0.798-3.705). The probability persist high in apneic snore compared with other kind of snore. The Odds ratio is 3.571 (95% CL 1.295-9.849, P < 0.05). The Odds ratio of cerebral infarction is highest in apneic snore compared with normal subjects (Odd ratio = 4.107, 95% CL 1.407-11.99, P < 0.01). CONCLUSION: Cerebral infarction is not obviously related with common snore. It is related significantly with the apneic snore. It indicated that snore is a possible cause of cerebral infarction. The possibility suffering from cerebral infarction is more in people especially in apneic snorer than no snorer.

Aged↗
PubMed2003

[Effect of low-dose triptorelin on pituitary down-regulation for patients undergoing in vitro fertilization and embryo transfer].

OBJECTIVE: To observe the effect of low-dose triptorelin in later luteal phase on pituitary down-regulation for patients undergoing IVF-ET. METHODS: One hundred and twenty patients were randomly divided into 2 groups(A and B). In group A, the patients were treated with triptorelin of 0.1 mg subcutaneously daily started on day 21 of menstrual cycle for 7 days. In group B, the patients were treated with long protocol of Buserelin intranasal spray. Comparisons were made between the groups in serum concentrations of FSH, LH, E2, T, PRL and P on the third day (D3) of superovulation cycle and on the day of hCG administration, highly purified follicle-stimulating hormone (HP) dosages, and so on. RESULTS: In group A, mean serum LH level on D3 and the day of hCG was (1.55 +/- 0.99) U/L and (2.70 +/- 1.45) U/L, E2 was (85.66 +/- 13.13) pmol/L and (5,451.31 +/- 1,900.61) pmol/L, respectively, significantly lower than those of group B (P < 0.001; P < 0.01; P < 0.05; P < 0.001). Mean HP(75 IU/Amp) dosage per treatment cycle in group A was 28.20 +/- 11.03 ampoules, significantly higher than 22.30 +/- 6.40 ampoules in group B(P < 0.001). CONCLUSIONS: Low-dose triptorelin in later luteal phase is effective for down-regulation of pituitary and produces more suppression on endogenous LH in pituitary than Buserelin long protocol.

Adult↗
PubMed2003

Synthesis of propenamides with anti-malarial activities and 3D-QSAR study.

AIM: To establish 3D QSAR model of propenamides with anti-malarial activities. METHODS: Chemical synthesis combined with comparative molecular field analysis (CoMFA). RESULTS: Generated QSAR models for activities of inhibiting chloroquine resistive malaria (W2) and chloroquine sensitive malaria (D6). CONCLUSION: The activity of anti-W2 depends mostly on steric interaction and the activity of anti-D6 depends on both steric and electrostatic interaction.

Acrylamides↗
PubMed2003

[Study on the safe edible of puffer fish resources].

In order to utilize the puffer fish resources furthest and to develop traditional diet culture, a project on the safe eating of puffer fish resources was conducted. The results showed that the distributing of teterodotoxin(TTX) in puffer body was not in uniformity. Owing that the content of TTX changes in different seasons and bodies, different methods were selected to crank out the delicious globefish food. The number of globefish food consumer has reached the amount to 121239 person-time since 1998. The technological process on teterodotoxin was proved reliable by mice bioassay and feeding trial in human.

Adolescent↗
PubMed2003

[A novel mutation causes congenital factor V deficiency].

OBJECTIVE: To investigate the gene defect in a hereditary coagulation factor V (FV) deficiency family. METHODS: The plasma FV actigen was measured by one-stage clotting assay. The FV antigen was assayed by Biotin-Avidin enzyme linked immunosorbent assay (BA-ELISA). The full length of exon 1 to exon 25 and the 5' untranslated sequence of FV genomic DNA were analyzed by polymerase chain reaction (PCR) and direct sequencing of the amplified fragments, meanwhile the defect was identified by T/A cloning sequencing. RESULTS: The plasma coagulant activity and amount of FV of the proband were marked deficient (1% and 1.54%, respectively). DNA sequence analysis for the proband revealed a causative mutation in a heterozygous status. It was one base pair deletion in exon 4 at nucleotide 675 inherited from her mother. CONCLUSIONS: A novel mutation in the FV gene was identified in the proband with congenital FV deficiency. The mutation was 675delA in exon 4 resulting in a frameshift and a premature termination codon.

Adolescent↗
PubMed2003

[Pharmacokinetics of osthole in rabbits].

AIM: To investigate the pharmacokinetics of osthole in rabbits and obtain the main pharmacokinetic parameters. METHODS: A simple high-performance liquid chromatography (HPLC) method was developed to study the pharmacokinetics of osthole in rabbits by joining an internal standard (paeonal). Methanol-water (80:20) was used as the mobile phase. According to the 3P87 pharmacokinetic program, the main parameters were calculated. RESULTS: The osthole pharmacokinetics conforms to a two compartment open model after i.v. administration, T1/2 alpha = 5.81 min, T1/2 beta = 42.2 min, K21 = 0.036 0.min-1, K12 = 0.045 0.min-1, K10 = 0.054 0.min-1, AUC = 235 mg.min.L-1, CLs = 0.043 0 L.min-1.kg-1, Vc = 0.780 L.kg-1. CONCLUSION: The pharmacokinetics of osthole after i.v. administration showed a rapid distribution and elimination process in rabbits.

Animals↗
PubMed2003

[A study on the epidemiology of permanent tooth carles in children aged 15 years in Anhui province].

OBJECTIVE: The first study on the epidemiology of oral health status in AnHui province was held. The purpose of this survey was to investigate the prevalence of dental diseases and the needs of dental treatment, so as to develop effective programs in providing dental care in children. METHODS: Non-proportional stratified cluster sampling was used for this study. RESULTS: The results showed that XDMFT in this group was 0.95, permanent caries prevalence rate was 39.78%, lower than that of the same age children in other 11 provinces in Mainland(P < 0.001). The proportion of DMFT was 86.78%(D), 4.30% (M), 8.92% (F), respectively. The caries number that needed dental care were 1842 teeth(0.78 teeth per person). CONCLUSION: The caries prevalence rate, the XDMF and the "F" were lower than the same age children in 11 provinces in Mainland and the "M" was higher.

Adolescent↗