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Biomedical subjects

Li Zhang

Publications and source records attributed to Li Zhang.

At least 379 records · Page 21Linked to original sources
PubMed2005

[Current status and development of monitoring on adverse reaction of traditional Chinese medicine in China].

With the wide usage of traditional Chinese medicine (TCM) and the diversity of its dosage-forms, the reports about adverse reaction (ADR) caused by TCM were gradually increased. As the cradle of TCM with tremendous manufacturing enterprise engaged for this purpose, to establish and perfect the TCM ADR monitoring is the problem that TCM industry should face to. In this paper, the key point of TCM ADR monitoring was made clear by analysing the current situation and the problems presented in TCM ADR monitoring in China, and indicated that the nowday developing goals of TCM ADR monitoring are to establish and perfect the technologic system for TCM ADR monitoring, strengthen the related basic research and enhance international communication and cooperation in this field.

Adverse Drug Reaction Reporting Systems↗
PubMed2005

[Preliminary study on human embryonic kidney cell line HEK-293 after porcine endogenous retrovirus infection].

OBJECTIVE: To assess the infectivity of porcine endogenous retrovirus (PERV) via in vitro infection of human embryonic kidney cell line HEK-293. METHODS: PERV particles were detected by immunoelectron microscopy. PERV DNA and mRNA were studied in HEK-293 24 hours after the infection using polymerase chain reaction and reverse transcriptase-PCR respectively. The PERV types were also analyzed. PERV-gag protein was observed by confocal microscopy. RESULTS: Retroviral particles were round under electron microscope. PERV-gag pol gene and gag protein were detected and expressed in the infected HEK-293 cells. The types of PERV were PERV-A and PERV-B. PERV-gag protein was also identified in the cytoplasm of infected cells by confocal microscopy. CONCLUSIONS: PERV is able to infect HEK-293 cell line in vitro; types of PERV-gag protein is also expressed as a result. Further studies are thus necessary in order to evaluate the possibility of xenozoonoses in pig-to-human xenotransplantation.

Animals↗
PubMed2005

Expression of matrix metalloproteinase 2 and its tissue inhibitor in oral squamous cell carcinoma.

The aim of this study is to investigate the expression of matrix metalloproteinase 2 (MMP-2) and the tissue inhibitor of metalloproteinase 2 (TIMP-2) in oral squamous cell carcinoma (OSCC) and adjacent normal tissues, and explore the role of MMP-2 and TIMP-2 in carcinoma metastasis and invasion. Expression of MMP-2 and TIMP-2 was evaluated in 40 cases with semi-quantitative reverse transcriptase polymerase chain reaction (RT-PCR) and immunohistochemical techniques. The values of MMP-2/beta-actin and TIMP-2/beta-actin in OSCC were significantly higher than those in adjacent normal tissues 2 cm and > or = 5 cm from carcinoma tissues (p<0.05). The results of immunohistochemical analysis show that the positive expression of MMP-2 and TIMP-2 protein in tumor tissues (60.0 and 52.5%) was higher than that in adjacent normal tissues 2 cm (30.0 and 22.5%) and 5 cm (22.5 and 25.0%) from cancer tissues, and the difference was significant p<0.05). The expression of MMP-2 and TIMP-2 was related to the differentiation degree of tumor cells, metastatic lymph node status and stage of carcinoma, but not related to the age and gender of patients, or location of the carcinoma. Therefore, MMP-2 and TIMP-2 may play important roles in the invasion and metastasis of OSCC.

Adult↗
PubMed2005

[Interleukin-1 polymorphisms in patients with aggressive periodontitis].

PURPOSE: To study whether interleukin-1 (IL-1) genotypes and/or alleles were associated with aggressive periodontitis (AgP). METHODS: Anti-coagulated peripheral blood samples were obtained from 122 AgP patients and 95 healthy controls. Genomic DNA was extracted from each sample. Single nucleotide polymorphisms (SNPs) at IL-1A +4845 and IL-1B +3954 were analyzed by standard polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) assay. The polymorphism of a variable number tandem repeat (VNTR) in intron 2 of IL-1RN was detected by PCR amplification and fragment size analysis. The genotypes and/or alleles distribution differences between two groups were analyzed by logistic regression analysis. RESULTS: The frequencies of A2+ genotype and allele 2 at IL-1A +4845 were significantly increased in male AgP patients compared with male controls (genotype: 18.0% vs 4.3%, adjusted OR=5.58, 95% CI=1.09-28.68, P=0.039; allele: 9.0% vs 2.2%, adjusted OR=4.97, 95% CI=1.01-24.50, P=0.049). CONCLUSIONS: The IL-1A +4845 polymorphism may be associated with AgP susceptibility in Chinese males.

Aggressive Periodontitis↗
PubMed2005

[An exploratory study of preoperative analysis on histocompatibility in porcine allo-kidney transplantation].

Human tissue typing methods were employed in developing a porcine allotransplantation model. 23 Chinese Sichuan White Pigs(2-3 months old, 17.5+/-4.6kg, with clear family background) were selected for tissue typing, ABO blood type cross reaction, complement-dependent cytotoxicity (CDC) cross reaction and one way mixed lymphocyte reaction (MLR). 6 pairs of swine that showed better matching results were selected as donors and recipients. Single-kidney orthotopic transplantation was conducted after removing both kidneys of the recipient. Five recipients showed low matching results (MLR ranging from 2175 to 3560, CDC from 1 to 4); of them, 2 died of operation, 2 died of acute renal tubular necrosis and accelerating rejection 4 days after operation respectively, and 1 died of acute renal tubular necrosis 4 days after operation. 6 recipients showed high matching results (MLR ranging from 982 to 1916, CDC from 2 to 4); of them, 1 died of anaesthesia during operation, 3 died of accelerating rejection and acute rejection 2 weeks after operation respectively, 1 had good kidney function, and 1 presented weak rejection 1 week after operation but the kidney function came back to normal afterwards. Human tissue typing methods could be adopted in developing the porcine model. Hyperacute rejection could be avoided by screening ABO blood type, CDC and MLR tests. However, based on these primary data, it was hard to evaluate the predictive values of CDC and one-way MLR for accelerating rejection, acute rejection and graft chronic dysfunction. Further research by expanding experiments in these aspects is still going on.

ABO Blood-Group System↗
PubMed2005

[Study on the active protein fractions from scorpii tegument].

OBJECTIVE: Extracting the total protein from scorpii tegument, investigating its effect on immune system by transformation of T/B cell. METHOD: Water-soluble protein(ST1) was extracted by distilled water method and salting-out method, while keratin(ST2) by deoxidization method. Sephadex G-50 was used to isolate the protein. The effects of components isolated by sephadex G-50 on T/B and NK cell were investigated. RESULT: The protein from scorpii tegument could increase the transformation of T/B cell distinctly in vitro, while no apparent effect on NK cell. CONCLUSION: Protein from scorpii tegument could modulate the immune system, which may offer a new way for people to find protein drugs.

Animals↗
PubMed2005

[Experimental study on xue-bao capsules against injury of radiation or cyclophosphamide in mice].

OBJECTIVE: To explore the effects of xue-bao capsules on injury of radio-or chemo-therapy in mice, in order to provide rationale behind clinical trials. METHOD: xue-xu (deficiency of blood) model in mice was induced by radiation or cyclophosphamide. Leucocyte (WBC), erythrocyte (RBC), hemoglobin (Hb) and platelet (Pt) in peripheral blood as well as CFU-E and CFU-Gm in bone marrow were counted. RESULT: CFU-E and CFU-Gm in normal mice were promoted by this drug. The reduction of WBC, RBC and Hb in peripheral blood as well as CFU-E and CFU-Gm in bone marrow owing to the 3.5 Gy of 60Co radiation were antagonized by the drug. It had also antagonized cyclophosphamide induced the reduction of WBC, RBC and Pt in peripheral blood. CONCLUSION: xue-bao capsules has the effects against the adverse reactions of radio-or-chemo-therapy.

Animals↗
PubMed2005

[Research advances in simulating regional crop growth under water stress by remote sensing].

It is of practical significance to simulate the regional crop growth under water stress, especially at regional scale. Combined with remote sensing information, crop growth simulation model could provide an effective way to estimate the regional crop growth, development and yield formation under water stress. In this paper, related research methods and results were summarized, and some problems needed to be further studied and resolved were discussed.

Biomass↗
PubMed2005

[Prostate sarcoma: a report of 14 cases].

OBJECTIVE: To discuss the diagnosis and effective treatment of prostate sarcoma. METHODS: We analysed the clinical materials of fourteen patients with prostate sarcoma treated in our hospital from Jan. 1991 to Jun. 2004. RESULTS: Prostate sarcoma accounted for 3.21% of all the prostatic malignant tumors treated in our hospital during that period. The average age was 39.5 years old (ranging from 17 to 62). Twelve cases came to our hospital because of dysuria. Large soft prostate tumors were found in all the patients in physical examination. The serum AKP, ACP and PSA levels were normal in all the 10 patients who had received the test. Pathological test revealed: 7 cases leiomyosarcoma, 1 case rhabdomyosarcoma, 4 cases fibrosarcoma, 2 cases spindle cell sarcoma. Different kinds of prostate sarcoma each his its own immuno-histochemical staining features. Two cases were at Ghavimi Stage I, 5 at Stage II, 3 at Stage III, and 4 at Stage IV. All the patients received surgery, chemotherapy and/or radiotherapy. One case failed to be followed up, 11 died 2 approximately 12 months after diagnosis. Two patients are still alive, 1 for 18 months without recurrence, and the other for through with relapse. CONCLUSION: Dysuria is always the first symptom of prostate sarcoma. DRE test may suggest prostate sarcoma but needle biopsy contributes to a definite diagnosis. The immuno-histochemical dyeing helps to differentiate the disease. The prognosis of prostate sarcoma is very poor and the main treatment is surgery followed by chemotherapy and/or radiotherapy.

Adolescent↗
PubMed2005

[Preparation and application of CuS/Cu2+ nanoparticles as a resonance light scattering probe].

Cuprum-enriched cuprum sulfide nanoparticles (CuS/Cu2+) were prepared. The nanoparticles are water-soluble. They could be used as a resonance light scattering (RLS) probe in the determination of human gamma globulin, which was proved to be a simple, rapid and specific method. In comparison with organic dye probes, these nanoparticles probes are brighter, more stable against photobleaching, and do not suffer from blinking. Under optimum conditions, the response is linearly proportional to the concentration of gamma globulin between 0.1 and 1.5 mg x L(-1), and the detection limit is 0.064 6 mg x L(-1).

Copper↗
PubMed2005

[Highly sensitive spectrofluorimetric determination of trace amounts of chromium (VI) with mono[6-o-(4-aminobenzoic acid)]-beta-cyclodextrin].

A novel fluorescence probe, mono[6-o-(4-aminobenzoic acid)]-beta-cyclodextrin(ACD), was synthesized, and a new sensitive fluorescence identification reaction was studied based on the reaction of Cr2O7(2-) with compound ACD in NaAc-HAc buffer medium at pH 4.90. Under optimal conditions the determination limit was 1.26 microg x L(-1). The linear range was 5.20-1040 microg x L(-1). This method has been applied to the determination of trace amounts of chromium (VI) in electroplating liquor, electroplating waste water with satisfactory results.

Chromium↗
PubMed2005

[Emerging of avian leukosis virus subgroup J in a flock of Chinese local breed].

Myeloid leukosis (ML) cases were first diagnosed in a chicken flock of Chinese local breed in Shan dong province. The main symptom included wasting, weight loss, anemia. It caused about 10% mortality of about 15000 birds at the age of 120-day. In the necropsy, gray-white nodules and protrusions in various sizes were commonly observed on the surface of the sternum, intestine and trachea. Almost all viscera tissues showed moderate to severe enlargement with diffuse gray-white nodules. Histological examination indicated that the tumor cells proliferated in tissues were myelocytes with eosinophilic granules in cytoplasm. In PCR with a pair of ALV-J-specific primers, 15 of 17 liver samples were positive. PCR product of one positive sample was sequenced and demonstrated 98.05% and 97.4% identity with ALV-J HPRS-103 strain at nuclei acid and amino acids level, respectively. By immunohistochemistry (IHC) technique with ALV-J monoclonal antibody, the most intense staining was in the tumor tissue, liver, spleen, kidney, bone marrow, and proventriculus. The results indicate that ALV-J already caused chickens infection and dead in Chinese local breed.

Amino Acid Sequence↗
PubMed2005

[A study on the epidemiology of permanent tooth caries in juvenile group in Anhui province].

OBJECTIVE: To survey the prevalence of dental caries and the needs for dental treatment in Anhui province. METHODS: The survey of prevalence of dental caries in group aged 12 and 15 years in Anhui province was conducted using the non-proportional, stratified, cluster sampling method. RESULTS: DMFT in 12-year and 15-year group was 0.58 and 0.95 respectively, and permanent tooth caries prevalence rate was 30.78% and 39.78% respectively, lower than that of same age of other 11 provinces in mainland China (P < 0.01). The constituent ratio of DMFT was 90.51% (D), 2.48% (M), 7.01% (F) in 12-year group and 86.78% (D), 4.30% (M), 8.92% (F) in 15-year group. CONCLUSIONS: When we controlled dental caries in 19.98% 12-years crowd and 22.8% 15-years crowd of Anhui province, we will had controlled the occurrence of 80% caries too in group aged 12 and 15 years.

Adolescent↗
PubMed2005

[Population characteristics of Coptis chinensis var. brevisepala in Anhui Province and its endangering mechanism].

The study on the distribution pattern, population size, and age structure of C. chinensis var. brevisepala in Anhui Province showed that C. chinensis var. brevisepala mainly distributed in the southern mountain areas such as Mts. Jiuhuashan, Guniujiang, Huangshan and Qingliang Peak. The habitats had two kinds, i.e., under woods and beside water ditches, both of which were shady and humid, with loose and acidic soils containing abundant organic matter. The shading degree surpassed 70%, air humidity reached 70% approximately 90%, and soil water content was more than 30%. The distribution pattern of existing C. chinensis var. brevisepala populations was clustering, with illogical age structure and small population size. It was suggested that the populations of C. chinensis var. brevisepala in Anhui Province were in an unsteady stage. Serious destroy of natural habitat and excessive digging and gathering adult individuals were the main causes of the rapid decrease of the individuals in some populations, which threatened the existence of C. chinensis var. brevisepala populations seriously.

Biodiversity↗
PubMed2005

[An epidemiological study on vaccine derived polio virus circle in Zhenfeng County of Guizhou Province].

OBJECTIVE: To analyze the event and cause of vaccine derived polio virus (VDPV) circle happened at Yaoshang Village, Wanlan Township, Zhenfeng County, Qianxinan Prefecture, Guizhou Province in August 2004. METHODS: Virus isolation was performed for stool specimens collected from two children cases with acute flaccid paralysis (AFP) reported at Yaoshang Village and 21 normal children under five years old in the same village. And, routine immunization coverage at the Village and AFP incidence and performance of AFP surveillance system in Zhenfeng County were investigated, as well. RESULTS: Vaccine derived polio virus 1 (VDPV1) was identified in the feces of two affected children at Yaoshang Village and three other normal children at the same village. Totally, seven cases of AFP have been underreported in Zhenfeng County since 2002. Routine immunization coverage was rather low at Wanlan Township, and nearly nil at Yaoshang Village. CONCLUSIONS: Oral polio vaccine (OPV) with attenuated live virus has been used in China for many years to prevent polio. Circulation of VDPV in Zhenfeng County issues a new challenge to eradication of polio. It is necessary to strengthen routine immunization and AFP surveillance to prevent occurrence of VDPV event.

Child, Preschool↗
PubMed2005

[Monitoring CML28 mRNA levels in patients before and after HSCT by real-time quantitative RT-PCR].

The purpose of this study was to establish a SYBR Green I real-time quantitative RT-PCR method for investigating the correlation between CML28 mRNA expression levels and relapse of leukemia after allo-hematopoietic stem cell transplantation (HSCT). pcDNA3.1HisA-CML28 plasmid had been constructed as the standard template. Serial monitoring of CML28 mRNA levels by SYBR Green I real-time quantitative RT-PCR technique was performed in 14 patients, including 10 patients with CML and 3 patients with AML, 1 patient with Ph(+) ALL. The results showed that the sensitivity of the established method was at 10(-4) (0.05 ng) level, with interassay variation and intraassay variation of standard samples both below 10%. The CML28 was highly expressed in AML and CML-BP or AP. CML28 level in newly diagnosed group was (6.58 +/- 2.34) x 10(-2), in pre-conditioning regimen group was (2.19 +/- 0.32) x 10(-2), in group that 1 month after HSCT was (1.35 +/- 1.28) x 10(-2), in group that 3 months after HSCT was (4.57 +/- 6.39) x 10(-3). CML28 can be detected 3 months after HSCT in 1 patient with CML-CP and 3 patients with CML-AP or BC. 2 of them with low level (<2 x 10(-2)) survived without relapse, the other 2 case with high level (>2 x 10(-2)) relapsed within one year, 1 case died and 1 case received the second time HSCT, CML28 level decreased rapidly after HSCT, but still higher than 2 x 10(-2) and relapse has taken place. The conclusions was made that CML28 mRNA level is obviously correlated with the development of leukemia. Serial quantification of CML28 mRNA levels are necessary for HSCT recipients, and more informative than a single detection. Using of this assay to evaluate MRD in the patients received HSCT is helpful for prediction of relapse.

Adolescent↗