DNA delivery to cells in culture using peptides.
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Animals↗
Biomedical subjects
Lei Zhang
Publications and source records attributed to Lei Zhang.
Overexpression of heparin-binding growth-associated molecule in malignant glioma cells.
The highly invasive and angiogenic characteristics of malignant gliomas depend on the production of growth factors and angiogenic factors. Heparin-binding growth-associated molecule (HB-GAM) is a secreted growth factor that is mitogenic for endothelial cells. To examine the expression profile of HB-GAM in malignant glioma cells, messenger ribonucleic acid (mRNA) expression was analyzed in 10 malignant glioma cell lines, two glioblastoma tissue specimens, and two normal brain tissue specimens by the reverse transcription-polymerase chain reaction. HB-GAM mRNA was expressed in all specimens including normal brain tissue specimens. Western blot analysis revealed that HB-GAM protein contents in glioma cell lines and glioblastoma tissues were 1.8 to 6.3 times higher than those in normal brain tissues. The effect of neutralizing anti-platelet-derived growth factor (PDGF) antibody was also examined on the production of HB-GAM in malignant glioma cells, since malignant glioma cells secrete PDGF that upregulates HB-GAM expression. Treatment of U251 and T98G glioblastoma cells with the anti-PDGF antibody did not affect the HB-GAM production. These results suggest that HB-GAM is overexpressed in malignant glioma cells and is involved in tumor growth.
Antibodies↗
Insulin sensitivity of muscle capillary recruitment in vivo.
We have reported that insulin exerts two vascular actions in muscle; it both increases blood flow and recruits capillaries. In parallel hyperinsulinemic-euglycemic clamp studies, we compared the insulin dose response of muscle microvascular recruitment and femoral blood flow as well as hindleg glucose uptake in fed, hooded Wistar and fasted Sprague-Dawley rats. Using insulin doses between 0 and 30 mU(-1). min(-1). kg(-1), we measured microvascular recruitment at 2 h by 1-methylxanthine (1-MX) metabolism or contrast-enhanced ultrasound (CEU), and muscle glucose uptake was measured by either arteriovenous differences or using 2-deoxyglucose. We also examined the time course for reversal of microvascular recruitment following cessation of a 3 mU. min(-1). kg(-1) insulin infusion. In both groups, whether measured by 1-MX metabolism or CEU, microvascular recruitment was fully activated by physiologic hyperinsulinemia and occurred at lower insulin concentrations than those that stimulated glucose uptake or hindleg total blood flow. The latter processes were insulin dose dependent throughout the entire dose range studied. Upon stopping the insulin infusion, increases in microvascular volume persisted for 15-30 min after insulin concentrations returned to basal levels. We conclude that the precapillary arterioles that regulate microvascular recruitment are more insulin sensitive than resistance arterioles that regulate total flow.
Animals↗
[Study on the application of the Chinese version of SF-36 scales and selection of interceptive cents for its grade range].
OBJECTIVE: To study the possibility of measuring quality of life by Medical Outcomes Study 36-Item Short-Form Health Survey scale and to subdivide grade range of Medical Outcomes Study 36-Item Short-From Health Survey (SF-36) total cents through a Quality of Life questionnaire among the elderly Chinese. METHODS: The quality of life of the 167 elderly Chinese in Suzhou was measured simultaneously by SF-36 and the Quality of Life questionnaire developed by Epidemiology Group of Geriatric Medicine Committee of China for the elderly. Validity and reliability were analyzed and subdivided the grade range of SF-36 total scores by Quality of Life questionnaire for the Chinese elderly. RESULTS: Eight common factors from factorial analysis were in accordance with their theoretical structure, and the cumulative contribution rates of the Quality of Life questionnaire for the elderly and SF-36 were 74.244% and 72.081%. The split-half reliability of the Quality of Life questionnaire for the elderly was 0.6676. The split-half reliability of SF-36 was 0.9384. In SF-36, the Cronbach's alpha coefficient of internal consistency reliability ranged from 0.81 to 0.89, which was satisfactory for group comparison except 0.63 for the social functioning and 0.42 for mental health scale and 0.69 for vitality scale. The Quality of Life questionnaire for old people seemed to have good validity and reliability but the SF-36 was better. The cent of the furthest truncation between the good quality of life and the medium one in the SF-36 was 117 with a Kappa value of 0.58. CONCLUSION: The SF-36 scale could be used for measuring and evaluating the quality of life for the Chinese elderly. The cent of the furthest truncation could provide reference to judge the level of the quality of life of the elderly.
Aged↗
[The use of BD-018 medical natural absorbable surgical suture in oral and maxillofacial surgery].
The oral and maxillofacial cutaneous or/and oral mucous wound of 20 cases were sutured with BD-018 medical natural absorbable surgical suture. Fifteen cases with class I wound and five cases with class II wound healed well after operation. BD-018 medical natural absorbable surgical suture adapts to oral and maxillofacial cutaneous or/and oral mucous wound suturing.
Adolescent↗
[Cloning and expression of immunoadjuvant molecule--CTB gene].
We cloned cholera toxin subunit B gene from 569B and M045 strain of Vibrio cholerae with polymerase chain reaction, constructed recombinant plasmid pCTB, and transformed pCTB into the prokaryotic cell strain JM109. The indentification was made by means of restriction enzyme analysis, polymerase chain reaction, DNA sequencing, SDS-polyacrylamine gel electrophoresis analysis and Western blot. The results indicate that we have amplified cholera toxin subunit B gene of 376 bp from Vibrio cholerae and hve constructed the recombinant plasmid pCTB, and we have affained the object amied at successful expression of 12 KD in the prokaryotic cell strain.
Adjuvants, Immunologic↗
Acute promyelocytic leukemia in a hemophilia A patient: a case report.
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Adult↗
Expression of vascular endothelial growth factor gene in primary cultured rat hepatocytes.
BACKGROUND: It is the key point for vascular endothelial growth factor (VEGF121) gene related therapy as to how to transfect and express the gene safely, effectively and repeatedly. This study was designed to investigate the VEGF121 transfection and expression in primary cultured rat hepatocyte. METHODS: After construction of vector internal ribosome entry site-enhanced yellow fluorescent protein (pIRES-EYFP)/VEGF121, the transfection and expression of the exogenous VEGF121 gene in primary cultured rat hepatocytes were observed through RT-PCR, Western blot and fluorescent microscopy. RESULTS: pIRES-EYFP/VEGF121 plasmid was constructed and transfected successfully into primary cultured rat hepatocytes, the transfection and expression of gene in primary cultured rat hepatocytes were examined by RT-PCR and Western blot, and yellow-green fluorescence was observed through a fluorescent microscope. CONCLUSION: The successful transfection and expression of plasmid pIRES-EYFP/VEGF121 in primary cultured rat hepatocytes provides a foundation for hepatocyte transplantation and gene therapy after modification of hepatocytes by the gene.
Animals↗
[Current situation and some intending problems of landscape spatial dynamic model].
Along with the rapid development of remote sensing, geographic information system, computer and other supporting technologies, model approach has become the major method of landscape dynamic study. Based on literature review, this paper analyzed the current situation and some intending problems of the development of landscape spatial dynamic model. Three kinds of models, i. e., stochastic landscape model, neighborhood rule model and landscape process model (including percolation model, individual-based landscape model and spatial ecosystem model) were mainly introduced, and their development status, existing problems and related improvement work were analyzed. The development of model test technologies, such as verification analysis, validation analysis, and sensitivity analysis, were also analyzed. Finally, this paper summed up six major problems that should be dealt with in the future development of the landscape spatial dynamic models. These problems included model arithmetic optimization, scale conversion, model complication and predigesting, model test and assessment, bringing forward of general model, and integration of the traditional model with social-economic factors.
Algorithms↗
[The research progress in ligament tissue engineering].
Ligament injury always has an unsatisfied outcome because of the poor blood supply and scar tissue formation. This may result in severe joint dysfunction. Tissue engineering, as a most prospective field, may provide an effective approach for the treatment of ligament injury. This paper has reviewed some recently published articles focusing on the sources of seed cells in ligament tissue engineering, application of growth factors, screening of scaffold materials with specific mechanical and biodegradable properties, and interaction between cells and scaffold materials. At present, what should be extensively studied are scaffolds with specific mechanical and biodegradable properties, and bioreactors providing three-dimensional culture microenvironment mimic in vivo.
Animals↗
[Enhancing antitumor activity of tumor-draining lymph node cells by autologous dendritic cells].
AIM: To enhance antitumor activity of tumor-draining lymph node(TDLN) cells by autologous dendritic cells(DCs) in-vitro. METHODS: Peripheral blood mononuclear cells(PBMCs) were isolated from the patients with gastric adenocarcinoma and induced with GM-CSF, IL-4 and TNF-alpha to obtain mature DCs which were then sensitized by autologous tumor lysate. TDLN cells were isolated and purified from lymph nodes of the patients and incubated with IL-2. Then we designed three experiment groups: (1)first group: TDLN cells activated by sensitized DCs; (2) second group:TDLN cells activated by autologous tumor lysate; (3)third group: TDLN cells alone, which were used as control. Cytotoxic activity of TDLN cells to gastric adenocarcinoma cell line KATO3 and human melanoma cell line A375 were compared between 3 groups. RESULTS: The cytotoxicity of TDLN cells activated by sensitized DCs to KATO3 cells was significantly stronger than that of TDLN cells activated by autologous tumor lysate or TDLN cells alone. In contrast, for A375 cells, cytotoxic activities of three TDLN cells had no significant difference. CONCLUSION: Specific antitumor activity of TDLN cells can be enhanced significantly by activated autologous DCs.
Adenocarcinoma↗
[Mechanical properties of preimpregnated glass fiber reinforced composite resins].
OBJECTIVE: To study the mechanical properties of two kinds of composite resins reinforced by preimpregnated glass fibers. METHODS: Sinfony and belleGlass composite resins were used to make standard samples (25 mm x 2 mm x 2 mm) with and without glass fibers. After all specimens were stored for 24 hours in distilled water at (37 +/- 1) degrees C, the flexural strength and flexural modulus were measured on a universal test machine. RESULTS: Both flexural strength and flexural modulus of Sinfony composite reinforced by the glass fibers became significantly increased, which were (555.76 +/- 67.31) MPa and (12.59 +/- 3.06) GPa respectively, and 4.5 and 2.5 times much more than those of the specimens without glass fibers (P = 0); the flexural modulus of belleGlass composite became significantly increased, which was (14.10 +/- 2.88) GPa, and 0.9 time much more than the specimens without glass fibers (P = 0), but the improvement of flexural strength was not significant. CONCLUSION: Glass fibers can improve the mechanical properties of composite resins, but the reinforcement effect is different between different resins.
Composite Resins↗
Montelukast modulates lung CysLT(1) receptor expression and eosinophilic inflammation in asthmatic mice.
AIM: To determine the expressions of cysteinyl leukotriene receptors, CysLT1 and CysLT2, in airway eosinophilic inflammation of OVA-induced asthmatic mice and the modulation by montelukast, a CysLT1 receptor antagonist. METHODS: Asthma model was induced by chronic exposure to ovalbumin (OVA) in C57BL/6 mice. The eosinophils in bronchoalveolar lavage (BAL) fluid and lung tissues were counted, IL-5 level in BAL fluid was measured, and CysLT1 and CysLT2 receptor mRNA expressions were detected by semi-quantitative RT-PCR. RESULTS: Montelukast (6 mg/kg, once per day for 20 d) significantly suppressed the increased eosinophils in BAL fluid and lung tissue, and increased IL-5 level in BAL fluid in OVA challenged mice. OVA challenge increased CysLT1 but decreased CysLT2 receptor mRNA expression. Montelukast inhibited the increased CysLT1 but not the reduced CysLT2 expression after OVA challenge. CONCLUSION: CysLT receptors are modulated immunologically, and montelukast inhibits up-regulation of CysLT1 receptor and airway eosinophilic inflammation in asthmatic mice.
Acetates↗
Early down-regulation of Bcl-xL expression during megakaryocytic differentiation of thrombopoietin-induced CD34+ bone marrow cells in essential thrombocythemia.
BACKGROUND AND OBJECTIVES: Essential thrombocythemia (ET) is a chronic myeloproliferative disorder with abnormal megakaryocyte/platelet production. Recent studies have found that Bcl-xL, as a member of the bcl-2 family of proteins that inhibit apoptosis, is essential in megakaryocytic differentiation. In this study the expression of Bcl-xL was evaluated during megakaryocytic differentiation in ET patients. DESIGN AND METHODS: To study the role of Bcl-xL in megakaryocyte differentiation, we evaluated the effect of small interfering RNA (siRNA) on the expression of Bcl-xL. CD34+ cells from patients with ET, chronic myeloid leukemia (CML), polycythemia vera (PV) and normal individuals were cultured in serum-free medium supplemented with thrombopoietin (TPO). Immunocytochemical staining and flow cytometric analysis were used to evaluate the Bcl-xL expression during megakaryocytic differentiation of CD34+ cells. RESULTS: When exposured to si-Bcl-xL, the percentage of K562 cells induced into megakaryocytes in 72 hours was lower than the corresponding percentage of control cells. CD41a+ cells from the three groups of patients and the control group were cultured. At day 10, the percentage of Bcl-xL- cells in CD41a+ cells from ET patients was 61.0+/-28.1%, which was significantly higher than that from patients with CML (2.5+/-20.9%), PV (33.6+/-10.0%) or control subjects (15.1+/-13.0%).] INTERPRETATION AND CONCLUSIONS: These results demonstrate that Bcl-xL is down-regulated early during in vitro differentiation of megakaryocytes from ET patients; this might reflect an early entry of megakaryocytes into a degenerating mature stage.
Adult↗
[Effect of tillage system on soil animal, microorganism and enzyme activity in paddy field].
A long-term experiment showed that under ridge-no-tillage, the amount of soil animal in 0 - 20 cm layer was 14700 ind. x m(-2), while under no-tillage and fallow in winter, paddy-upland rotation, and conventional tillage, it was 10450, 7950 and 6275 ind. x m(-2), respectively. Soil microbial biomass and microbial biomass N were more in spring and autumn, and less in summer. Soil enzyme activity was higher in surface soil layer and lower in bottom soil layer. The amount of soil animal, microbial biomass and microbial biomass N and soil enzyme activity was in order of ridge-no-tillage > paddy-upland rotation > no-tillage and fallow in winter > conventional tillage. The results also indicated that ridge-no-tillage was advantageous to improve soil ecological environment and soil fertility in paddy field.
Actinomyces↗
[Therapeutic effects of a low molecular weight peptide from cattle spleen on masugi nephritis in rats].
OBJECTIVE: To investigate the therapeutic effects of a low molecular weight peptide KZ-36 from cattle spleen on masugi nephritis in rats. METHODS: The rabbit serum of anti-rat kidney was initially prepared, and then injected into normal rats to induce the formation of masugi nephritis. In this model, KZ-36 was administrated (i.p.) at the dosage of 50 microg/kg/d for 2 weeks, while saline and hydrocortisone were respectively used as negative and positive controls. The pharmacodynamic parameters were collected. RESULTS: After KZ-36 administration for one week, the protein output in 24 h urine remarkably decreased, and was 5.0 +/- 4.1 mg for two weeks, which was much less than 17.2 +/- 14.5 mg, a counterpart in saline control (P<0.01). KZ-36 could also reduce the serum level of creatinine and improve the renal pathological injury. CONCLUSION: KZ-36 has obvious therapeutic effects on masugi nephritis in rats.
Albuminuria↗
[Meta-analysis on the relationship between tobacco smoking, alcohol drinking and p53 alteration in cases with esophageal carcinoma].
OBJECTIVE: To investigate the relationship between tobacco smoking, drinking and p53 alteration in esophageal carcinoma. METHODS: Literature on the relationship between p53 alteration in esophageal carcinoma and tobacco smoking, drinking through Meta-analysis were reviewed. RESULTS: In 14 selected papers related to tobacco smoking, pooled odds ratio (OR) of tobacco smoking with P53 overexpression and p53 alteration were 1.99 (95% CI: 1.30- 3.06) and 1.64 (95% CI: 1.13 - 2.37), respectively (P < 0.05). Pooled OR of tobacco smoking with p53 mutation was 1.11 (95% CI: 0.47 - 2.76) (P > 0.05). In 11 selected papers on alcohol drinking, pooled OR of drinking with P53 overexpression, p53 mutation and p53 alteration were 1.30 (95% CI: 0.83 - 2.04), 1.13 (95% CI: 0.67 - 1.90) and 1.22 (95% CI: 0.87 - 1.72) respectively (P > 0.05). CONCLUSION: There were significant relations between tobacco smoking and p53 alteration while there were no significant relations between alcohol drinking and p53 alteration.
Alcohol Drinking↗
[Effect of autoskin grafting in fault hypodermis wound of granulation excision on full-thickness burn healing].
OBJECTIVE: To explore the mechanism of full-thickness burn wound healing with autoskin grafting in fault hypodermis wound of granulation excision and to evaluate its effect. METHODS: By the techniques of clinical observation, histopathology, immunohistochemistry, TEM and FCM, we observed changes of the activity and structure of grafted skin and the granulation tissue, collagnous fiber,microvessels,the ultramicrostructure of fibroblasts and the expression of basic fibroblast growth factor (bFGF) in the base of autoskin grafting in fault hypodermis wound in burned adult minipigs (Group A), and compared with traditional method of autoskin grafting on the basilar fibrous tissue wound of scraped partly granulation being (Group B) and control group (Group C, without treatment except de-fur). RESULTS: The grafted skin survived after 3 days of operation, and it had less injury and higher proliferative index (PI) in group A than in group B. The hyperplasia of granulation tissue and vascular endothelial and the expression of bFGF were more evident in group A. After 5 days, the proliferation of endothelial cells and granulation and the protein synthesis of fibroblasts were more active in group A, and at this moment, fresh collagen appeared and proliferated more actively in group B. After 7-14 days, epidermic structure and dermic microvascular density became normal gradually, the granulation on grafting base matured and transformed into fibrous connective tissue in group A. The same change deferred about 2 days in group B. After 21 days, the above pathologic change in group A was less than that in group B. After 30-60 days of operation, Group A achieved much less contraction and transfiguration than Group B, and the grafted skin was tender and movable. CONCLUSION: Autoskin grafting in fault hypodermis wound of granulation excision has a better effect than traditional operation.
Animals↗