[Hepatic stellate cell apoptosis and mitochondrion].
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Animals↗
Biomedical subjects
Lei Wang
Publications and source records attributed to Lei Wang.
[Experimental research on immunological rejection of brain tissue allograft].
AIM: To investigate the occurrence of rejection in brain tissue transplantation. METHODS: Ventral mesencephalon(VM) single cell suspensions from newborn rats were allografted into the striata of Parkinson's disease model recipient rats. Surviving animals were sacrificed at 2, 4 and 6 wk after transplantation, and the brain tissues were stained with HE or immunocytochemically to inspect the expression of tyrosine hydroxylase (TH) and major histocompatibility complex class II antigens (MHC II). RESULTS: High MHC II expression was observed within and around the allografts compared with control at 2 wk after grafting. MHC II expression then decreased at 4 wk and at 6 wk only few immunopositive cells remained. The number of TH positive neurons was low at 2 wk, but increased at 4 and 6 wk after transplantation. CONCLUSION: The brain is not an "immunologically privileged site" and graft rejection exists in brain tissue transplantation. This rejection maybe induced by MHC II. Therefore it is necessary to use immunosuppressants in brain transplantation.
Animals↗
[Effects of a novel recombinant polypeptide from the sea anemone Anthopleura sp. on left ventricular function in dogs with acute cardiac insufficiency].
OBJECTIVE: To observe the effects of recombinant hk2a, a novel neurotoxin from the sea anemone Anthopleura sp., on left ventricular function of dogs with acute cardiac insufficiency. METHODS: Canine models of acute cardiac insufficiency were established by rapid ventricular pacing, in which the left ventricular ejection fraction (LVEF) was measured by Acuson ultrasound systems (Sequoia 512) at 0, 5, 15, 30 and 60 min, respectively, after intravenous injection of 30 microg/kg recombinant hk2a. The response of the canine models to hk2a treatments was observed in comparison with that of the dogs treated with Cedilanid (as positive control) and saline (as negative control). RESULTS: Intravenous injection of recombinant hk2a caused an immediate and significant increase in LVEF in the canine models of acute cardiac insufficiency (P<0.05), and the effect maintained for more than 30 min without significant effect on heart rate. Recombinant hk2a possessed such merits as quicker onset and greater potency in comparison with Cedilanid. CONCLUSION: Recombinant hk2a may significantly increase LVEF of the dogs with acute cardiac insufficiency.
Animals↗
[The cDNA cloning of conarachin gene and its expression in developing peanut seeds].
In order to clone the genes of peanut seed storage proteins, a cDNA library of mid-maturation stage cotyledons of peanut (Arachis hypogaea L. Shanyou 523) was constructed. After immunoscreening with polyclonal antibody against conarachin from peanut seeds, two cDNA sequences named Ahy alpha and Ahy beta of conarachin genes were obtained. BLAST these sequences in GenBank showed that they share high homology with the genes of legume 7S globulins. One of the sequences (Ahy beta) was found to be identical to part of the published complete cDNA sequence of the peanut allergen, Ara h1 p17. The other fragment (Ahy alpha) was almost identical to parts of the published complete cDNA sequence of the peanut allergen, Ara h1 p41b. Results of southern analysis show that they belong to a small gene family (the result is not shown). These sequences were expressed only in developing peanut seeds. To isolate the genes involved in seed storage protein and to understand the gene expression during peanut seed development, the sequences of 414 ESTs were determined and further analyzed by BLAST searches and categorized functionally. Five rESTs (representative ones) of arahin, two rESTs of conarachin and six rESTs of conglutin-like proteins were isolated. 17.0% of total transcripts isolated in this study are involved in peanut seed storage proteins.
Allergens↗
[Screening of human scFv against IL-8 from phage antibody library].
AIM: To screen human scFv against IL-8 from phage antibody library. METHODS: IL-8-His fusion protein was expressed in E.coli BL21 (DE3) transfected with prokaryotic expression vector pRSET-IL-8 and was purified by affinity chromatography. Specific antibody was screened by 3 rounds of panning of phage antibody library with the fusion protein. The antigen binding activity and DNA sequences of positive clones were determined and analyzed. RESULTS: After 3 rounds of panning, 2 positive clones were obtained which could bind IL-8 specifically. DNA sequence analysis showed both of the 2 VH genes belonged to human IgG VH3 subgroup, and the Vlambda gene belonged to human VlambdaDPL5 and VlambdaDPL2 subgroups,respectively. CONCLUSION: It is feasible to obtain human anti-IL-8 antibody by phage display technique, which provides the basis for further research on psoriasis and other related diseases.
Antibodies↗
[Complications of transcatheter interventional occlusion of ventricular septal defects].
OBJECTIVE: To investigate the complications and their prevention in patients with perimembranous ventricular septal defect (VSD) experiencing transcatheter interventional occlusion. METHODS: From July, 2002 to May, 2004, totally 262 (138 males, 124 females) perimembranous VSD patients underwent transcatheter interventional occlusion in the department. The age ranged from 2 to 18 years (mean 9.3 +/- 5.8 years), and the body weight ranged from 11.0 to 65.0 kg (mean 30.5 kg). The implanted occluder with imported Amplatzer eccentric perimembranous VSD occluder, domestic double-disk, domestic eccentric perimembranous VSD and PDA occluder were attempted in 12, 212, 28 and 6 cases, respectively. Among them, 3 cases were complicated with ductus arteriosus, 4 with atrial septal defects and 1 with patent ductus arteriosus plus atrial septal defect. The associated defects were treated with other occluders at the same time. RESULTS: The diameters of VSD measured by transthoracic echocardiography before occlusion and by ventriculography during the procedure ranged from 2.7 to 13 mm (mean 6.5 mm) and from 1.3 to 14.0 mm (mean 6.8 mm), respectively. The defects in 256 cases (97.7%) were successfully occluded. The implant occluder was from 4 to 16 mm in size. Serious complications occurred in 8 cases (3.1%), including 5 cases of high degree atrioventricular block (AVB) (2.0%), 2 cases of hemolysis (0.8%) and 1 case of displacement of the occluder (0.4%). Amongst 5 cases of high degree AVB, permanent pacemaker was implanted only in 1 case due to high degree AVB 50 days after treatment, whereas the others all recovered. Two cases of hemolysis were completely recovered. One case of occluder displacement was emergently transferred to cardiac surgery. Other complications included residual shunt during 6 month follow-up in 1 case (0.4%), minor aortic regurgitation in 5 cases (2.0%), minor tricuspid regurgitation during 6 month follow-up in 4 cases (1.6%), and accelerated idioventricular rhythm or accelerated atrio-ventricular junctional tachycardia combined with atrioventricular interference-dissociation by ECG in 75 cases (29%) but they recovered after 3 - 5 days of corticosteroid treatment. Forty-one cases (16%) complicated with complete or incomplete right bundle branch block, of whom 50% recovered during follow-up. Still, there were 4 cases (1.6%) who were complicated with complete left bundle branch block but were all recovered during follow-up and 26 cases (10%) who were complicated with intraventricular block. There were no Significant difference in complications between cases who accepted imported Amplatzer occluders and domestic occluders. CONCLUSION: Transcatheter interventional occlusion is a safe, effective and ideal method with low incidence of serious complications for perimembranous VSD. Long term follow-up and clinical research should be carried out to improve the level of VSD interventional therapy.
Adolescent↗
[Randomized controlled trial on haiguiyuyang capsule in the treatment of duodenal ulcer].
OBJECTIVE: To assess the efficacy and safety of haiguiyuyang capsule in the treatment of duodenal ulcer (also diagnosed as weiwan pain and hanrecuoza syndrome according to the theory of TCM). METHODS: This is a multi-center clinical trial cooperatively conducted from May 2003 to March 2004 in accordance to the principle of informed consent, to the criteria for subject inclusion, exclusion, discontinuation, and to the relevant guidelines for evaluating the therapeutic effects of new TCM drugs. The design of double-blind, double-dummy and randomized controlled trial was adopted. 438 patients were randomized to the Test group (n = 330, treated with haiguiyuyang capsule) and to the Control group (n = 108, treated with ranitidine). The therapeutic course for both groups was 6 weeks. RESULTS: Regarding the efficacy in treating duodenal ulcer, in the Test group, the Marked Efficacy Rate was 66.37% and the Total Efficacy Rate was 82.13%; in the Control group, the Marked Efficacy Rate was 68.61% and the Total Efficacy Rate was 93.34%; there was no significant difference between the two groups (P>0.05). Regarding the efficacy in treating the syndrome diagnosed by TCM, in the Test group, the Marked Efficacy Rate was 70.31% and the Total Efficacy Rate was 93.34%; in the control group, the rates were 71.29% and 91.66% respectively; there was no significant difference between the two groups (P>0.05). Besides, regarding the abatement of distention and fullness of stomach after treatment, the haiguiyuyang capsule was better than ranitidine (P<0.05). No adverse side-effect was observed. CONCLUSION: The efficacy of haiguiyuyang capsule in treating duodenal ulcer is similar to that of ranitidine. No obvious adverse effect of it was observed in this trial.
Adolescent↗
[Influence of mannitol on cerebral blood flow of post-resuscitation children as detected by transcranial Doppler ultrasound].
OBJECTIVE: To detect the influence of mannitol on cerebral blood flow of post-resuscitation children by transcranial Doppler (TCD). METHODS: The blood flow changes of left side middle cerebral artery (MCA) and extracranial internal carotid artery (EICA) were monitored by TCD in 21 post-resuscitation children. TCD waveforms, peak velocity (Vp), diastolic velocity (Vd), mean velocity (Vm) and pulsitility index (PI) of MCA and EICA were monitored daily and 30 minutes before and after the first dose of mannitol. Glasgow scores were estimated in the meantime. The patients were divided into 2 groups according to the patterns of diastolic flow. Patients in group I showed no diastolic flow or retrograde diastolic flow (n = 9), and patients in group II had positive diastolic flow (n = 12). RESULTS: Vp, Vd, Vm, PI of MCA and EICA in group I patients had no significant change after the administration of mannitol and the Glasgow scores were much lower than that of group II patients (P < 0.05). TCD waveforms showed no improvement in group I patients and their outcomes were poor. Vd, Vm and Vp of MCA and Vd of EICA in group II patients increased, PI of MCA decreased (P < 0.05), while Vp, Vm, PI of EICA had no significant change. TCD waveforms recovered within one week. All the patients in this group survived. CONCLUSIONS: The results suggested that mannitol could improve cerebral perfusion in patients with mild brain damage. These patients usually had increased diastolic blood flow in the early stage. Lack of or retrograde diastolic flow of TCD waveforms might be associated with severe brain damage with poor outcome. Mannitol had no effect on improving cerebral blood flow in these patients.
Adolescent↗
[Detection of SARS-associated coronavirus N protein by time-resolved fluoroimmunoassay].
OBJECTIVE: To develop a method for quantitative detection of severe acute respiratory syndrome (SARS)-associated coronavirus (SARS-CoV) N protein by timed-resolved fluoroimmunoassay (TRFIA). METHODS: Using a monoclonal antibody (mAb) against SARS-CoV N protein, screened by SARS-CoV N protein and matching experiment, a method for quantitative detection of SARS-CoV N protein by TRFIA was established on the basis of double sandwich enzyme-linked immunosorbent assay (ELISA) and evaluated against the ELISA kit. RESULT: The measurement range of the assay was 0.02-150 ng/ml with a sensitivity of 0.02 ng/ml, the coefficient of variability within runs of 3.3%;-6.2%;, and coefficient of variability between days of 5.3%;-9.6%;. The results of detection were consistent between ELISA and TRFIA. CONCLUSION: TRFIA is a new, sensitive and specific immunoassay for detecting SARS N protein with potential value in clinical applications.
Antibodies, Monoclonal↗
[Transcriptional regulation of noeAB from Sinorhizobium meliloti 042BM].
The expression regulation of S. meliloti 042BM noeAB was studied. The results showed that trigonelline could not elevate the level of noeAB expression, which indicated that these genes are not regulated by nodD2. Since association of nodD3 and syrM could not change the level of the genes expression, they aren't also controlled by nodD3-syrM system. However, induction of luteolin resulted in 16 times increase of noeAB expression, which indicated that noeAB was regulated by nodD1. Most interestingly, more than 30 times increase in its expression was observed on TY medium without any flavonoid. Thus, it was suggested that noeAB may be controlled by other unknown factors.
Alkaloids↗
[Construction of the genomic library of Halobacillus sp. D8 and isolation of the glycine betaine transporter betH gene].
Halobacillus sp. D8 is a sporing-forming, gram-positive moderately halophilic bacterium which could tolerate up to 25% (W/V) NaCl. A genomic library of Halobacillus sp. D8 was constructed using pUC18 as vector, and 9000 recombinant plasmids were obtained. By dot blot hybridization, colony PCR and DNA sequencing, the entire glycine betaine transporter betH gene was isolated from the constructed library. Inspection of the sequenced 4.3 kb DNA region revealed the presence of three ORFs. The putative ORF of betH is 1515bp long, encoding a 505-residue protein (BetH) with a calculated molecular mass of 56.1kD. Hydrophobicity plot analysis of BetH indicated a transmembrane protein containing 12 transmembrane regions. Homology searches for BetH of strain D8 in the GenBank using the BLAST program revealed significant sequence identities to other glycine betaine transporters: the putative glycine betaine transporter of O. iheyensis (64% identity), OpuD of B. subtilis (51% identity), BetH of H. trueperi (49% identity), BetL of L. monocytogenes (48% identity), BetM of M. halophilus (43% identity) and the putative glycine betaine transporter of B. halodurans (44% identity).
Amino Acid Sequence↗
[The expression of gene related to salt tolerance from Sinorhizobium meliloti 042BM in Escherichia coli and purification of its fusion protein].
A 1.9kb DNA fragment related to salt tolerance of S. meliloti strain 042BM containing two open reading frames were obtained by PCR amplification and ligated into shuttle vector pBBR1-MCS2. The complementation experiment showed that ORF2 is related to salt tolerance and named as rstA gene. Then the gene was cloned into the expression vector pThio-HisA, B and C, respectively, and recombinant expression vectors pGSA, pGB and pGC were constructed, and transformed into E. coli Top10. Inducing by IPTG and analyzing with SDS-PAGE, the fusion protein encoded by pGSA was obtained,and it is 36% content of whole cell protein. It was isolated and purified by affinity chromography on ProBond, and the inclusion body precipitated by saturated sulfate ammonium, and 95% purity of fusion protein was obtained. The final product displayed a single band with a corresponding molecular weight 43kD in SDS-PAGE, and was verified by the Western blot.
Bacterial Proteins↗
[Cloning, deletion and functional analysis of noeA from Sinorhizobium meliloti 042BM].
042BM noeA was obtained by PCR. It is identical to that of S. meliloti 1021 at 99% level, and similarity of their NoeA is 97%. In addition, it was found that this protein shares significant homology with the SAM-dependent methyltransferase of Mesorhizobium sp. BNC1 (32% similarity), and the similarity of its 303-362 region to the 160- 220 region of Ll11 methyltransferases of E. coli (PrmA) is 41%. Compared to 042BM, the noeA deletion mutant 042BMA-Km showed different degrees of increase in number of nodule, fresh weight of nodule and plant top dry weight on alfalfa cultivars of Putong Zihua, Baoding, Ningxia, Baifa and Aohan, but decrease on Milu. However, this mutant has no significant change in ability to nodulate cultivars of Huanghou and Zahua. Hence, noeA is involved in alfalfa cultivar-specific nodulation.
Acyltransferases↗
[Combined bio-process for treatment of municipal wastewater containing Cu2+].
A combined bioprocess consisting of biosorption section with P. putida 5-x cell as biosorbent followed by a SBR is developed to treat Cu2+ containing municipal wastewater. The optimal preparing technique of P. putida 5-x cell as biosorbent is studied. Under the optimal condition, adsorption capacity of the P. putida 5-x cell to Cu2+ reached 87.3 mg x g(-1). The performance of the combined bioprocess for treating Cu2+ containing wastewater is assessed. The experimental results indicate that after treatment by the biosorption section, the Cu2+ concentration in wastewater reduced to the level that did not inhibit COD removal efficiency of subsequent SBR activated sludge process, although it still affectes the COD adsorption capacity of activated sludge. In terms of COD removal, the biosorption section is efficient for reducing Cu2+ concentration to provide biodegradable wastewater for subsequent SBR activated sludge process.
Adsorption↗
[Influence of nitrogen source NH4 Cl concentration on curdlan production in Alcaligenes faecalis].
The effect of initial ammonium chloride level on production of curdlan in Alcaligenesfaecalis was investigated. It was found that ammonium chloride was the limiting substrate for cell growth during the batch fermentation process. However, the cell growth and curdlan production could not be enhanced by solely increasing the initial ammonium chloride level. The pH drop in the broth due to the consumption of ammonium chloride also effected the cell growth and curdlan production. By simultaneously increasing the initial ammonium chloride concentration and implementing an optimal pH control strategy, which is to control pH at 7.0 in the growth phase, and then shift to 5.6 in the production phase, the biomass and curdlan production in batch fermentation were increased markedly. If the initial ammonium chloride concentration was increased from 1.1 g/L to 3.6 g/L, biomass concentration of 7.2 g/L was obtained, and the final curdlan concentration reached 30.5 g/L, which was 51.7% higher than that of the former case. As the cell growth was improved due to the increase of the initial ammonium chloride concentration, the agitation speed and aeration rates must be enhanced to suit the higher oxygen uptake requirement. However, as curdlan molecules is subject to the structural breakage due to the high shear stress at higher agitation speed, an overall optimal condition for both productivity and quality of curdlan should be considered comprehensively.
Alcaligenes faecalis↗
[Analysis of spontaneous reperfusion in acute myocardial infarction and its effect on short-term prognosis].
OBJECTIVE: To analyse the clinical and angiographic characteristics of spontaneous reperfusion (SR) in AMI, and to evaluate its effect on short-term prognosis. METHODS: 112 consecutive AMI patients without intravenous thrombolytic therapy received emergent coronary angiography and primary PCI. The patients were divided into SR group (antegrade TIMI grade 2-3 flow) and non-SR group (antegrade TIMI grade 0-1 flow). The clinical, angiographic and prognostic features of the patients were analyzed. RESULTS: 31 patients (27.7%) were in SR group, and there was no significant difference in base-line clinical characteristics between the two groups. Compared with non-SR group, peak values of CK and CK-MB, Ventricular wall motion abnormality and mortality were lower in SR group, ejection fraction was higher in SR group. Logistic regression analysis showed that there was good correlation between SR and peak value of CK, collaterals, ventricular wall motion abnormality and pre-dilation in PCI. CONCLUSION: SR decreased infarction size, improved heart function and reduced 30-day mortality.
Aged↗
[Dissipation mechanism of excessive energy in umbilical orange under phosphorus deficiency and strong light stress].
The dissipation mechanism of excessive energy of umbilical orange under phosphorus deficiency and strong light stress was studied with solution culture method. The results showed that under phosphorus deficiency and strong light stress, the photosynthetic pigment content, net photosynthetic rate (Pn), photorespiration rate (Pr), maximum fluorescence (Fm), photochemical efficiency (Fv/Fm) and electron transmit rate (ETR) of umbilical orange were all declined, while original fluorescence (Fo) and Pr/Pn ratio were raised. The fast-phase (qNf) of non-photochemical quenching of chlorophyll fluorescence was decreased, while the middle-phase (qNm) and slow-phase (qNs) were increased. After treated with DTT, Fo turned higher, but qNm and qNs were much lower than the control (H2O), while qNf did not have any significant change. Phosphorus deficiency and strong light stress aggravated photoinhibition of photosynthesis, which in turn started up several dissipation mechanisms in umbilical orange leaf.
Citrus sinensis↗
[CD34+ antigen expression relating to prognosis in acute myeloid leukemia].
To explore CD34(+) antigen expression in new diagnosed acute myeloid leukemia (AML) and analyze the prognosis for CD34(+) AML patients, the expression of antigen CD34 in 238 AML patients was detected by indirect immunofluorescence assay. The results showed that CD34 in 92 out of the 238 patients (38.7%) were positive, there was relationship between the CD34(+) expression and FAB subtypes (M(0), M(1)), and no CD34(+) expression was observed in M(3) subtypes. The complete remission rate of CD34(+) AML patients was 32%, which was lower than that of CD34(-) AML (61%). The lymphoid-associated antigen (CD7) was significantly increased in CD34(+) AML patients, compared with CD34(-) patients (P < 0.05). It is concluded that CD34(+) AML patients show poor prognosis and lower CR rate. The detection of CD34 expression is of some value in predicting prognosis in AML.
Adolescent↗