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Biomedical subjects

Lei Wang

Publications and source records attributed to Lei Wang.

At least 181 records · Page 10Linked to original sources
PubMed2006

[Experimental study on the phenotype of human embryonic lung fibroblasts transfected with Ad-BMP-2].

OBJECTIVE: To investigate the effects on the phenotype, especially the mineralization ability of human embryonic lung fibroblasts(HELFs) by transfection with adenovirus vector ecoding human bone morphogenetic protein-2 gene (Ad-BMP-2). METHODS: The HELFs were primarily cultured, then transfected with Ad-BMP-2. The morphologic characteristics of the cells were observed. The cell proliferation, alkaline phosphatase activities, BMP-2 protein expression, and the mineralization ability were detected with the methods of MTT, ALP staining, Western blot, and alizarin red S staining, respectively. RESULTS: After transfection, the shape of HELFs changed from silm spindle to multifigure, the cells became bigger than before. The colonies changed from unilaminar into multilaminar. The proliferation of HELFs was severely inhibited after transfection. An obvious BMP-2 lane was shown in Western blotting. Most cells presented positive in ALP staining, and large number of nacarat mineralized nodes were observed after alizarin red S staining. CONCLUSION: HELFs were capable of transforming into osteoblast-like phenotype, and were endowed with the ability of mineralization while being transfected with Ad-BMP-2.

Bone Morphogenetic Protein 2↗
PubMed2006

[Detecting the haplotype of three Y-STR loci by fluorescent multiplex amplification].

OBJECTIVE: To establish a fluorescent multiplex PCR system for typing Y-STR loci Y-GATA-A7.1, DYS456 and DYS443, and investigate their haplotype frequencies in Chinese Han population. METHODS: 203 unrelated males of Han population living in Zhengzhou were typed by fluorescent multiplex amplification system and ABI 3100 genetic analyzer. RESULTS: In Zhengzhou Han population, 5,6 and 6 different alleles were observed for Y-GATA-A7.1, DYS456 and DYS443 loci, and their gene diversity (GD) were 0.669 2, 0.583 9 and 0.705 3 respectively. A total of 44 different haplotypes formed by these three loci was identified and the haplotype diversity (HD) reached 0.952 3. CONCLUSION: The fluorescent multiplex system for these three Y-STR loci will be very powerful for forensic individual identification and paternity testing in Chinese Han population.

Alleles↗
PubMed2006

[Experiment study on anoxic phosphate accumulation with nitrite].

Biological phosphorus removal with nitrite was studied in an Sequencing Batch Reactor. The results showed that nitrite can be used as electron acceptor in denitrifying phosphorus removal. Feed mode of nitrite had significant influence on denitrifying phosphorus removal. Anoxic phosphorus assimilation rate could be reached 10.44 mg/(g x h) and percentage of anoxic phosphorus assimilation amount was more than 97%. Granular sludge with denitrifying phosphorus removal activity was cultivated in an SBR. The effects of different operational conditions on formation of granular were studied such as COD loading, settling time, HRT and so on. The average dimension of granular was 315 microm. Its water content was at 96.78%-98.14%, specific gravity 1.0064-1.016 , SVI of sludge 25 40 mL/g, COD removal loading could be reached to 1 5 kg/(m3 x d), nitrogen and phosphorus removal efficiency was more than 90%.

Bacteria, Aerobic↗
PubMed2006

[Capability of Cu2+ adsorption on cell surface of Pseudomonas putida 5-x].

Analysis of Cu2+ adsorption on cell surface of a Gram-negative bacterium P. putida 5-x was carried out. Isolated cell envelopes possess fivefold more Cu2+ adsorption capacity than that of the fresh intact cells. Experimental results also indicate that the content of the peptidoglycan layer, the outer membrane and inner membrane in the cell envelope is in order of inner membrane > outer membrane > PEG layer, while their Cu2+ adsorption capacity is in order of PEG layer > outer membrane > inner membrane. The total contribution of the separated PEG layer material to Cu2+ adsorption is no more than 15% part, and the cell outer membrane and inner membrane contribute about 30%-35% and 25%-30% part, respectively, to Cu2+ adsorption by the cell envelope. The relatively high phospholipid content in the outer wall might result in its higher adsorption capacity to Cu2+, and leads to a higher adsorption capacity of the cell envelope of P. putida 5-x.

Adsorption↗
PubMed2006

[Surgery therapy of Crohn disease complicated with fistula].

OBJECTIVE: To investigate the surgical treatment of Crohn disease(CD) complicated with perforation or fistula. METHODS: The clinical data of 24 CD cases complicated with perforation or fistula treated in our department from Jan. 1980 to Apr. 2005 were analyzed retrospectively. RESULTS: There were 24 cases of CD complicated with perforation or fistula among 181 CD patients,and the incidence of perforation or fistula was 13.3%. Steroid treatment before perforation was given in 8 patients, while not in 9 patients and unclear in 7 patients. Eighteen patients had single perforation, 4 patients two free perforations and 2 patients more than three extra-abdominal fistula. Emergency operation was performed in 8 cases and selective operation in 16 cases. Five cases had postoperative complications including incision infection in 2, intra-abdominal abscess in 2 and stomal fistula in one case, and were all cured by surgical drainage and anti-infection treatment. The morbidity rate was 20.8%. The postoperative mortality rate was 12.5% (3/24). Eighteen patients were followed up from 6 months to 18 years, and the 5-year recurrent rate was 16.7%. The 10-year reoperation rate was 33.3%. CONCLUSIONS: Steroid use is not the main cause of perforation or fistula in CD. Perforation or fistula is the definite indication for surgical treatment.

Adolescent↗
PubMed2006

[Sequence of Escherichia coli O11 O-antigen gene cluster and identification of molecular markers specific to O11].

Escherichia coli O11 belongs to Shiga toxin-producing Escherichia coli (STEC), which can cause food-borne disease, hemorrhagic colitis, and hemolytic-uremic syndrome (HUS) in humans. Because of its character of specificity, the O-antigen gene cluster provides the best material for the selection of molecular markers which can be used for rapid genotyping of bacterial strain. In this study, the E.coli O11 O-antigen gene cluster was amplified by Long-range PCR and was sequenced using Shotgun-sequencing approach. Twelve open reading frames were assigned functions on the basis of homology in the E. coli O11 O-antigen gene cluster, including UDP-N-acetyl glucosamine-4-epimerase gene (gne), genes responsible for the biosynthesis of GDP-L-fucose (gmd, fcl, gmm, manC, manB), glycosyl transferase genes, O-unit flippase gene (wzx) and O-antigen polymerase gene (wzy). By polymerase chain reaction against representative stains for all the 166 E. coli and 43 Shigella O serotypes, two genes and four pairs of primers were identified to be specific to E. coli O11. Further PCR was done to detect E. coli O11 from the environmental specimens, and the sensitivities for detecting E.coli O11 from the pork and dejecta specimens were 0.25 cfu/g and 2.5 x 10(3) cfu/g, respectively. Moreover, eight probes were designed and proved to be unique to E. coli O11, which provides the basis for a sensitive test of the rapid detection of E. coli O11 by DNA microarray method.

Biomarkers↗
PubMed2006

[Extraction, identification and primary investigation of adhesion specificity of Lactobacillus sp].

Lactobacillus L15, a strain of Lactobacillus sp. isolated from the intestinal tract of healthy Paralichthys olivaceus, and Lactobacillus acidlophilus ATCC4356 were used to investigate their specific adhesions. It was shown that cell surface proteins of two strains could be extracted by 5 mol/L lithium chloride( LiCl). Two surface proteins with molecular weights of 61.8 kDa and 54.6 kDa were identified to mediate the adhesion to intestinal mucus of Paralichthys olivaceus and Common carp respectively by the method of Western blotting, and the two novel proteins were named as MAPPpo1 and MAPPcc. In Lactobacillus acidlophilus ATCC4356, two proteins of 43.0 kDa and 63.3 kDa were identified to mediate the adhesion to intestinal mucus of Paralichthys olivaceus. Nevertheless, only 43.0 kDa protein was identified to mediate the adhesion to intestinal mucus of Common carp. Furthermore, the mucus proteins which participated the adhesion as the bacteria receptors were identified by the same method and it was found that L15 and ATCC4356 had the same receptors.There were 29.7 kDa and 30.3 kDa proteins in the intestinal mucus of Paralichthys olivaceus and only the 26.2 kDa protein was testified in the intestinal mucus of Common carp. It is demonstrated that the adhesion of Lactobacillus to mucus is dependent on the strain specificity, as well as host specificity.

Adhesins, Bacterial↗
PubMed2006

[Isolation and characterization of gamma-TMT gene promoter from Arabidopsis thaliana].

Vitamin E (Tocopherols) is lipid-soluble antioxidants and essential for human health. Gamma-tocopherol methyltransferase (delta-TMT), one of the key enzymes in tocopherol biosynthetic pathway in plants, converts delta,sigma-tocopherols into alpha,beta-tocopherols. In this study, we isolated the 1552 bp promoter of Arabidopsis TMT gene. The promoter was fused with GUS reporter gene and this expression cassette was introduced into wild Arabidopsis thaliana by Agrobacterium-mediated transformation. GUS staining shows that GUS gene is expressed in leaves, stems and flowers, with the highest expression in young leaves, stamens and stem apices, while not observable in roots, seeds and siliques. The data indicate that gamma-TMT gene promoter is likely to be expressed preferentially in some of the tissues of Arabidopsis.

Arabidopsis↗
PubMed2006

[The effects of antidepressant treatment on efficacy of antihypertensive therapy in elderly hypertension].

OBJECTIVE: To explore the effect of antidepressant treatment on antihypertensive therapy of senile hypertension. METHODS: 138 cases of senile hypertension complicating with depression were studied. 103 senile hypertensive patients without depression in the same period served as controls. The patients were randomly divided into 3 groups as A, B and C for study. 12.5 mg/d hydrochlorothiazide and 30 mg/d release-controlling tablets of nifedipine were orally administrated as basic antihypertensive medication each group. Besides, 20 mg/d fluoxetine was additionally given to groups A and one tablet of almitrine l/d and 20 mg oryzanol 3/d were given to group B. The treatment lasted 12 weeks. RESULTS: In comparison with pre-treatment, the blood pressure at base line in sitting position and the average circadian SBP and DBP monitored with 24-hour ambulatory blood pressure monitoring (ABPM) was remarkably decreased in group A with statistical significance (P < 0.01). These blood pressure parameters in group B were also decreased as compared ith the pretreatment level brt with less statistical significance (P > 0.05). It was also noted tha with the addition of antidepressant fluoxetine, the manifestations of depression ere alleviated as shown by Hamilton depression (HAMD) scale. untoward reactions such as nausea, perspiration and skin rash were noted in a few patients, but none withdrew from the study. CONCLUSIONS: Antidepressant and antianxiety treatment is of benefit to the antihypertensive therapy in senile hypertensive patients with complications of depression and/or anxiety neurosis. Based on the results of this study, it is suggested that routine administration of antidepressants and antianxiety drugs should be carried out in senile hypertensive patients complicating with depression or anxiety.

Aged↗
PubMed2006

Cloning and characterization of a novel sperm tail protein, NYD-SP28.

In this study, a gene coding a novel human sperm tail protein named NYD-SP28 was cloned and characterized using a complementary DNA (cDNA) microarray. Its expression was 3.5 times higher in human testis than in fetal testis, and very high in human spermatozoa. The full length of NYD-SP28 cDNA was 1798 bp and encoded a 484-amino-acid protein. Motif analysis revealed that the protein contained a cluster of phosphorylation sites, N-glycosylation sites and N-myristoylation sites. Immunohistochemical analysis of normal human testes showed that NYD-SP28 was expressed in the cytoplasm of spermatogenic cells but not in interstitial cells. The EGFP-NYD-SP28 fusion protein was also localized in the cytoplasm of transfected 7721 cells. In human spermatozoa, NYD-SP28 immunoreactivity was detected in entire sperm tail. Using the two-dimensional (2-D) gel electrophoresis and immunoblotting technique, NYD-SP28 was found to be post-translationally modified during sperm capacitation. In conclusion, these results suggest that NYD-SP28 is a new human sperm tail protein and might play an important role during sperm capacitation.

Adult↗
PubMed2006

[Protective effects of Xuebijing injection on kidney in rats with sepsis].

OBJECTIVE: To investigate the protective effects of the integrated traditional Chinese and Western medicine Xuebijing injection on the kidney in rats with sepsis. METHODS: Sepsis model was reproduced in rats with cecal ligation and puncture (CLP). Eighty healthy Wistar rats were randomly divided into four groups: control group, sham-operation group, CLP model group, and Xuebijing group. The two latter groups were divided into four groups of 2, 8, 24, 48-hour subgroups with 8 rats in each subgroup. Serum levels of creatinine (Cr), blood urea nitrogen (BUN), Na(+), K(+) were measured. beta (2)-microglobulin (beta (2)-MG) was determined by radioimmunoassay, urine level of Cr, Na(+), interleukin-6 (IL-6) levels in renal tissue, and fractional excretion of sodium (FENa) were determined by enzyme linked immunoadsorbent assay (ELISA). Light microscopy was used to assess the pathological changes in kidney. RESULTS: Compared with CLP model group,the level of beta (2)-MG was significantly decreased at 2 hours in Xuebijing group (P<0.01). Similarly, serum BUN content, renal IL-6 level were lowered at 8 hours, and serum contents of BUN as well as Cr were also decreased in Xuebijing group than those in model group at 48 hours after CLP (P<0.05 or P<0.01). CONCLUSION: Xuebijing injection possesses the protective effects on the kidney in rats with sepsis, which may be related with the synthesis and release of IL-6.

Animals↗
PubMed2006

[Characteristics of absorption spectra of phytoplankton].

Nine typical phytoplankton species were chosen and cultivated under two temperatures (20 and 15 degrees C) and two illumi- nations (7000 and 1100 lux), and their absorption spectra at different growth period were measured. Firstly, singular value decomposition was used on the matrix, which is composed of the autoscaled spectra data. The S1/msigma(i=1)Si was used to compare the similarities of spectra. Twenty-five representative spectra were obtained for the nine species of phytoplankton. Among them, there are one spectrum for each of Isochrysis galbana and Chaetoceros Debilis, two for each of Platymonas helgolanidica and Chaetoceros curvisetus, three for Gymnodinium sp., and four for each of Alexandrium tamarense, Prorocentrum dentatum, Skeletonema costatuma and Synechococcus sp. Then, feature extraction was processed to obtain the characteristic spectra, including seven incontinuous wavelengths that have great ability to differentiate species. They are 340. 5-420. 5 nm, 423. 5-431 nm, 440.5-525.5 nm, 760.5, 763.5, 769.5 and 856.5 nm. Consequently, they form characteristic spectra. The discriminant result is 80%.

Animals↗
PubMed2006

[Pegylated interferon alpha 2a in treating chronic hepatitis B patients].

OBJECTIVE: To evaluate the efficacy and safety of pegylated interferon alpha 2a (PEG-IFN alpha-2a) in treating patients with chronic hepatitis B. METHOD: Seventy-two patients with chronic hepatitis B were assigned to a PEG-IFN alpha-2a (experimental) group (n=42) and an interferon alpha (control) group (n=30) randomly. Each patient in the experimental group received 180 microg PEG-IFN alpha-2a every week. Each patient in the control group received 500 MU interferon alpha every day. All the patients were treated for 48 weeks, and then were followed for another 48 weeks with no treatment. RESULTS: At the end of the 12th week, the rate of HBeAg negative cases was 30% in the PEG-IFN alpha-2a group, which was much higher than in the control group (x2 = 4.162, P < 0.05). The values of HBeAg and the log value of HBV DNA in the PEG-IFN alpha-2a group were much lower than the values before the treatment (t = 2.689, t = 4.080, P <0.01), but there was no difference between before and after treatment in the control group ( t = 1.229, t = 1.009, P > 0.05). At the end of the 24th week, the rate of HBeAg negative cases in the PEG-IFN alpha-2a group was much higher than that in the control group (x2=6.190, P < 0.05). The value of HBeAg and the log value of HBV DNA in the PEG-IFN alpha-2a group were much lower than in the control group (t=2.215, t=2.122, P < 0.05). At the end of the 48th week, besides the reduction mentioned above, the rate of cases with HBeAg/antiHBe seroconversion and normalization of ALT and complete responsiveness in the PEG-IFN alpha-2a group were all much higher than those in the control group (x2=5.771, x2=5.617, x2=5.308, P < 0.05). At the end of 48 weeks with no treatment, all the parameters mentioned above in the PEG-IFN alpha-2a group were much better than those in the control group and they remained so, but they were different in the control group (x2=11.943, t=3.439, t=6.111, x2=9.930, x2=9.522, x2=7.920, P < 0.01). Nine patients in the PEG-IFN alpha-2a group had liver biopsies before their treatment and also at the end of their treatment. The expressions of HBsAg and HBcAg were decreased at the end of the treatment. The rate of expression of HBsAg in the liver tissues before the treatment was 88.9% but only 22.2% at the end of the treatment (x2=8.001, P < 0.01). The rate of expression of HBcAg in the livers before treatment was 66.7% but only 33.3% at the end of the treatment. Before and at the end of the PEG-IFN alpha-2a treatment, there were no significant changes in the degrees of inflammation and fibrosis and the quantity of collagen in the liver tissues. Three patients in the PEG-IFN alpha-2a group (10%) were HbsAg negative. Two of them were found so at the end of 32 weeks with treatment and one patient was found at the end of 24 weeks with no treatment, but there were no HBsAg negative patients in the control group. The adverse reactions that occurred in the PEG-IFN alpha-2a and in the control groups were similar. CONCLUSION: PEG-IFN alpha-2a was effective in inhibiting HBV replication. The effect of PEG-IFN alpha-2a was lasting. PEG-IFN alpha-2a was well tolerated during our treatment.

Adolescent↗
PubMed2006

[Clinicopathological characteristics and prognosis of patients with recurrent colorectal cancer].

OBJECTIVE: To investigate the clinicopathological characteristics and prognosis of patients with recurrent colorectal cancer. METHODS: Clinicopathological characteristics and postoperative survival of 235 patients with recurrent colorectal cancer after radical resection were retrospectively analyzed, and was compared with that of 993 patients with radical resection. RESULTS: The overall 5- and 10-year survival rates after radical resection were 67.7% and 55.8%, respectively. The 5- and 10-year survival rates in patients with recurrent colorectal cancer was 43.9% and 28.1% (P=0.000), respectively. Among patients with recurrent colorectal cancer, the 5- and 10-year survival rates of the patients underwent second radical resection were 50.2% and 32.7%, while the 5- and 10-year survival rates of the patients without second surgery were 25.8% and 0, respectively (P=0.000). On univariate analysis, postoperative recurrence was associated with age at diagnosis, cancerous ileus, ascites, lymphs nodes involvement, gross types, infiltration of tumor and Duke's stage. Multivariate analysis revealed that cancerous ileus, ascites, gross types and Duke's stage were independent predictive factors for postoperative recurrence. CONCLUSION: Cancerous ileus, ascites, gross types and Duke's stage were independent predictive factors for recurrence and metastasis of colorectal cancer after radical resection. The 5- and 10-year survival rates and quality of life could be improved by second radical resection.

Adult↗
PubMed2006

[Aboveground biomass of Tamarix on piedmont plain of Tianshan Mountains south slope].

Based on the geo-morphological and hydro-geological characteristics, the piedmont plain of Tianshan Mountains south slope was classified into 4 geo-morphological belts, i.e., flood erosion belt, groundwater spill belt, delta belt, and the joining belt of piedmont plain and Tarim floodplain. A field investigation on the Tamarix shrub in this region showed that there was a significant difference in its aboveground biomass among the four belts, ranged from 1428.53 kg x hm(-2) at groundwater spill belt to 111.18 kg x hm(-2) at the joining belt of piedmont plain and Tarim floodplain. The main reason for such a big difference might be the different density of Tamarix shrub on different belts. Both the Tamarix aboveground biomass and the topsoil's salinity were decreased with increasing groundwater level. Groundwater level was the main factor limiting Tamarix growth, while soil salinity was not.

Biomass↗
PubMed2006

[Designing and realization of the dynamic filter in B mode ultrasonic scanning].

This paper introduces the design and realization of the FIR dynamic filter by EP1C6Q240 of Cyclone of Altera Corporation, showing the hardware diagram of the filter and the process of calculating parameters, and compares and analyzes the B mode ultrasound images created by using the dynamic filter and by using the constant filter.

Algorithms↗
PubMed2006

[Molecular characterization of O-antigen gene cluster of Escherichia coli O23 reference strain and identification of UDP-N-acetylglucosamine 4-epimerase].

Lipopolysaccharide (LPS) is one of the major components of the outer membrane of gram-negative bacteria. It is an amphipathic molecule composing of lipid A, a core oligosaccharide and an O-specific antigen. O-antigen, which is a repeat unit polysaccharide, is a major contribution to the antigenic variability of the bacterial cell surface. Genes involved in O-antigen biosynthesis are generally found to be clustered between the housekeeping genes galF and gnd on the chromosome of E. coli. E. coli O23 is one of the enterotoxigenic E. coli causing pediatric diarrhea in the developing world. The O-antigen gene cluster of E. coli O23 type strain was amplified by long-range PCR using primers based on galF and gnd and then sequenced. Except for galF and gnd, seven open reading frames were identified and assigned functions on the basis of their similarity to those from available databases. The seven genes include a UDP-N-acetylglucosamine 4-epimerase gene (gne), the O-antigen polymerase gene (wzy), the O-antigen transferase gene (wzx) and four glycosyltransferase genes (orf2, orf4, orf5, orf6). The UDP-N-acetylglucosamine 4-epimerase (Gne) was identified by mutation and complementation complement tests. The structure of Gne was predicted by the homology modeling method, and the active sites were also analyzed. The phylogenetic and structural analysis showed that the Gne derived from the common ancestor with E. coli O23 Gne were UDP-GlcNAc/UDP-GalNAc epimerases. The specific DNA used for rapid molecular genotyping for E. coli O23 was also identified.

Base Sequence↗