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Biomedical subjects

L Zhou

Publications and source records attributed to L Zhou.

At least 289 records · Page 16Linked to original sources
PubMed1999

[Surgical treatment of trigeminal neurinomas].

OBJECTIVE: To investigate the best surgical approach to remove of trigeminal neurinomas (TNs). METHODS: 75 patients with TNs in Huashan Hospital were reviewed retrospectively. RESULTS: In early group (1978 - 1984) conventional intradural approaches were used, and in late group (1985 - 1995) an epidural approach via the skull-base craniotomy was utilized. Total removal of was achieved in 58% (20/35) in the early group and 80% (32/40) (P < 0.05) in the late group. Temporary or permanent cranial nerves morbidities were 62.7%, 37% in the early group and 28.1%, 10% (P < 0.01) in the late group, respectively. CONCLUSIONS: The best surgical approach with microsurgical technique for removal of TNs is epidural approach or epiduro-transduro-transtentorial approach via a skull-base craniotomy except the tumor only confined to the posterior fossa.

Adolescent↗
PubMed1999

[Neuronavigation for the resection of intracranial cavernous angiomas].

OBJECTIVE: To evaluate the use of the Stealth Station neuronavigator in conjunction with preoperative computerized tomography (CT) in the resection of intracranial cavernous angiomas (CAs). METHODS: The Stealth Station neuronavigator was used to provide real-time correlation of the operating field and computerized images in 6 patients with CAs. All of them suffered from epileptic seizures. RESULTS: Neuronavigation-guided lesionectomy with removal of hemosiderin deposition, gliosis and calcification in all cases was performed precisely. Mean fiducial error, predicted accuracy at 10 cm, and sustained accuracy ranged from 1.65 to 4.53 mm, 1.82 to 3.28 mm, and 0.50 to 3.45 mm, respectively. CONCLUSIONS: The Stealth Station neuronavigator is reliable and accurate in the resection of CAs.

Adolescent↗
PubMed1999

[Liver metastasis in colorectal cancer and tumor angiogenesis].

OBJECTIVE: To investigate the association between tumor angiogenetic activity and metastatic potential of colorectal cancer (CRC) retrospectively. METHODS: The microvessel density (MVD) and the level of vascular endothelial growth factor (VEGF) expression were examined immunohistochemically from 61 specimens obtained by surgical resection of CRC. RESULTS: MVD was (27.0 +/- 8.4) and the expression of VEGF was (59.75 +/- 12.36)%. Both were significantly higher in the group of liver metastasis, and in the group of lymph node metastasis (P < 0.01, P < 0.05). Survival analysis indicated a significant difference between increasing MVD group and the control group (P < 0.01). CONCLUSIONS: VEGF-mediated angiogenesis plays an important role in liver metastasis of CRC, and MVD appears to be a new prognostic factor for the prediction of outcome in patients with CRC.

Colorectal Neoplasms↗
PubMed1999

[A study on family aggregation of type 2 diabetes mellitus].

OBJECTIVE: To estimate the genetic susceptibility and its relative risk (RR) in the first degree relatives of type 2 diabetes mellitus (DM) through family aggregation analysis. METHODS: A population-based case-control study including 654 pedigrees (363 index cases and 291 controls) was carried out. RESULTS: The prevalence rate in the first degree relatives of DM probands was 3.94%, significantly higher than that in the controls (1.09%), with a relative risk of 3.62 (chi(2) = 36.5, P < 0.001). The RRs in different consanguineal relatives were all higher than 3.0. The younger was the probands at diagnosis for DM, the higher its prevalence rates and RRs among their relatives was and the more likelihood that more than one case of DM occurred in them. CONCLUSION: Significant family aggregation and higher genetic susceptibility were found in the first degree relatives of DM, on whom prevention and treatment for DM should be focused.

Adult↗
PubMed1999

[Determination of triptophenolide in Chinese herb leigongteng by TLCS].

OBJECTIVE: To establish a method for the determination of triptophenolide in Chinese herb Leigongteng (Tripterygium wilfordii Hook. f.). METHOD: TLCS was used. The sample was purified with a column and developed upwardly with CHCl3-EtOEt(2:1), sprayed with Keddle testing solution and scanned. The content was calculated with external two-point method. RESULT: The linear range was 2.1 micrograms-10.5 micrograms, r = 0.998. CONCLUSION: The method is proper for the quality control of Leigongteng.

Chromatography, Thin Layer↗
PubMed1999

[Flavonoids from Beijing propolis].

OBJECTIVE: To separate and identify the active flavonoids from Beijing Propolis. METHOD: The flavonoids were isolated by column chromatography with silica gel and polyamide, purified with Sephadex LH-20, and elucidated on the basis of NMR, MS, UV, IR and CD spectra. RESULT: Six kinds of flavonoids were obtained and elucidated as I: pinostrobin, 5-hydroxy-7-methoxy-flavanone; II: pinocembrin, 5, 7-dihydroxy-flavanone; III: tectochrysin, 5-hydroxy-7-methoxy-flavone; IV: chrysin, 5, 7-dihydroxy-flavone; V: izalpinin, 5-hydroxy-7-methoxy-flavonol and VI: galangin, 5, 7-dihydroxy-flavonol. CONCLUSION: Compounds I, II, III and V were found for the first time from Beijing Propolis.

Flavanones↗
PubMed1999

[Determination of nervonic acid in oral tonic by GC].

A method for determination of nervonic acid in oral tonic has been developed by gas chromatography. The samples were derivatized by BF3-CH3OH. Dinonyl phthalate was used as the internal standard. The calibration curve was linear over a range from 0.3 g/L to 3 g/L with a correlation coefficient of 0.9994. The average recovery of the method was 97.04% and the RSD was 0.90%. The method has been successfully applied to quality control of nervonic acid in oral tonic.

Administration, Oral↗
PubMed1999

[Perspectives of effect on new electrode technology with implantable motor prostheses for stimulating peripheral nerves].

This paper explores approaches to electrode technology and stimulating techniques that selectively activate specific portions of a peripheral nerve trunk with much less implanted hardware than is required with present motor prosthesis systems. The first section reviews the vulnerability of nerves to both mechanical and electrical factors and suggests ideas on how these findings might be utilized in future electrodes. The second section presents results on longitudinally oriented intrafascicular electrodes for nerve stimulation recording. The third section reviews results on longitudinal intrafascicular electrodes for nerve stimulation and recording.

Animals↗
PubMed1999

[Beaded molecule imprinted polymer for stereo isomer separation].

Beaded molecule imprinted polymer (MIP) was made by suspension polymerization. Particles with the size of 50-70 microns in diameter were collected and evaluated in HPLC mode to separate stereo isomers. Stereo isomers cinchonine and cinchonidine were successfully discriminated with selectivity factor of 2.89 and resolution factor of 0.76. Stereo selectivity of the MIP was found to come from both the interaction between the analyte and carboxyl group on the MIP and the similarity between the stereo structure of imprinted molecule and the MIP. The thermal analysis results showed that the MIP had high thermal stability with initial thermal decomposition temperature of 320 degrees C. The pore volume of the MIP was 0.1849 mL/g, the specific surface area was 126.84 sqm/g and the average pore diameter was 5.8 nanometer. Scanning electron microscopy showed that MIP had perfect spherical morphology.

Chromatography, High Pressure Liquid↗
PubMed1999

[Analysis of Lyapunov exponents of heart rate variability in diabetics].

This paper presents an analysis of Lyapunov exponents of ten normal persons and ten patients with diabetes mellitus by the definition of the Lyapunov exponents, the length evolution and the area evolution. The results show that the Lyapunov exponent of diabetic is clearly lower than that of normal. It can indicate the diabetic with less number of electrocardiographic data.

Adult↗
PubMed1999

[Studies on the parasites of Paris polyphylla var. yunnanensis].

Two bacteria and three fungi were isolated from the hypogeal stems of Paris polyphylla var. yunnanensis. The bacteria were identified as Bacillus cereus and Pseudomonas alcaligenes, and the fungi were identified as Periconia sp., Pachnocybe albida and Hormomyces paridiphilus. The results on liquid culture of B. cereus, P. alcaligenes and H. paridiphilus indicated that the colloidization and polysaccharide content increasing in hypogeal stems of P. polyphylla var. yunnanensis were due to the extracellular polysaccharide secretion of the parasitical fungus H. paridiphilus.

Bacillus cereus↗
PubMed1999

[Genotype and sequence analysis of merozoite surface protein 1 of Plasmodium falciparum isolates in Yunnan province].

AIM: To identify the genotype of merozoite surface protein 1 of Plasmodium falciparum in Yunnan Province and explore the polymorphism of MSP1 genes in geographical characteristics and genetics. METHODS: Nested polymerase chain reaction was applied to genotyping of P. falciparum isolated in Yunnan Province. Some of parasite alleles were sequenced by dye primer cycle sequencing. RESULTS: In 30 P. falciparum infections, 38 different alleles were found. Of them, the dominant allele was a variant of MAD20, while was K1 less and the RO33 was few. In addition, incidences of mixed allele infections were observed. Sequence analysis showed that DNA sequences of MAD20, K1 and RO33 alleles from Yunnan were highly homologous with those of international standard strains, respectively. CONCLUSION: Composition and sequence characteristics of P. falciparum arasite population in the endemic area can be detected by genotyping with MSP1 as genetic marker, which would be useful for the prevention and treatment of malaria.

Alleles↗
PubMed1999

[Study on the correlation between the cyclin D1 and laryngeal squamous cell carcinoma].

OBJECTIVE: To study the correlation between the expression level of Cyclin D1 and the laryngeal squamous cell carcinoma (LSCC). METHOD: 35 LSCC cases testified pathologically were included in the study. The samples obtained from each tumor tissue and adjacent normal mucosa were detected to see the expression level of Cyclin D1 with the method of RT-PCR and light density half quantive analysis. RESULT: The expression level of Cyclin D1 was significant higher in almost all turmor tissues than in the adjacent normal mucosae (P < 0.05). Overexpressed carcinomas were more frequently associated with lymph node metastases (P < 0.05). A significant association between the expression level of Cyclin D1 and TNM was found. The higher T stage, the higher the expression level of Cyclin D1 (P < 0.01). The expression level of low histological grade carcinomas was lower than that of the high histological grade carcinomas, but the difference had no statistical significance. No association between overexpression of Cyclin D1 and sex, age was found too. CONCLUSION: The result indicates that the expression level of Cyclin D1 in LSCC may be a useful marker of prognostic significance and it provide a molecular biology basis for treating the LSCC patients.

Aged↗
PubMed1999

[Phase II clinical study of a new anticancer drug boanmycin].

OBJECTIVE: To evaluate the efficacy of boanmycin (BAM) in patients with advanced cancers. METHODS: A multicenter phase II clinical study on BAM was conducted on 105 cases received BAM as single agent, and 220 cases were treated with combination chemotherapy containing BAM. RESULTS: The total response rate was 35.0% for single agent and 64.2% for combination chemotherapy group in advanced cancers. The response rate for single agent in malignant lymphoma, cancer of head and neck and breast cancer was 66.7%, 65.0% and 37.5%, respectively; and for combination chemotherapy group in malignant lymphoma, breast cancer, head and neck cancer, esophageal cancer and lung cancer was 88.5%, 62.5%, 50.0%, 47.2% and 45.8%, respectively. The main side effects with mild or moderate fever, chill, myalgia, gastrointestinal reactions, skin pigmentation as well as hardening of skin at injection site. CONCLUSIONS: BAM therapy was effective in malignant lymphoma, cancer of head and neck and other cancers.

Adolescent↗
PubMed1999

[Efficient expression of human papillomavirus type 16 (HPV16) capsid proteins L1 and L2 in E. coli].

OBJECTIVE: To study the expression of HPV16 capsid proteins L1 and L2 in E. coli. METHODS: The plasmids pET-30a-L1 and pET-30a-L2 were constructed from the pET-30a vector, and transducted into the BL21. The fusion proteins 6 x His-L1 and 6 x His-L2 were expressed when induced by adding of IPTG. SDS-PAGE and Western blot analyses were used to detect the expressed proteins. RESULTS: Fusion proteins 6 x His-L1 and 6 x His-L2 were expressed efficiently in E. coli, the Mr were about 60,000 and 97,000 respectively, 6 x His-L1 degraded more than 6 x His-L2. CONCLUSION: L1 and L2 proteins were expressed at a high level in prokaryotic expression system, and 12 protein were more stable than L1 protein.

Capsid Proteins↗
PubMed1999

[The reconstruction of laryngeal function in subtotal laryngectomy with preservation of arytenoid cartilage].

OBJECTIVE: To evaluate subtotal laryngectomy with preservation of arytenoid cartilage to reconstruct laryngeal function in T3 glottic or supraglottic laryngeal cancer. METHODS: Subtotal laryngectomy with preservation of arytenoid cartilage to reconstruct laryngeal function was reviewed in 20 patients with T3 laryngeal cancer(3 cases with glottic cancer and 17 cases with supraglottic cancer). Recurrent laryngeal nerve was preserved during surgery. The mucous membranes between cricoid cartilage and pharynx were sewed up accordingly. RESULTS: All patients had restored phonation and swallowing functions. The 3- and 5-year survival rates were 94.1% and 91.8% respectively. Decannulation rate was 95.0%. CONCLUSION: Subtotal laryngectomy with preservation of arytenoid cartilage is recommended for T3 laryngeal cancer.

Aged↗
PubMed1999

[Mechanism of arsenic hydride generation and atomization in low temperature atomic fluorescence spectrometry].

With a hollow cathode lamp as exciting source, the optimum working parameters for the determination of As were selected. By adopting two hydride-generators to generate hydride and H2 simultaneously, the mechanism of AsH3 generation was studied. Experimental results show that the atomization process of AsH3 might be carried out mainly by collision with hydrogen radical.

English Abstract↗