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Biomedical subjects

L Yman

Publications and source records attributed to L Yman.

At least 19 recordsLinked to original sources
PubMed2001

Standardization of in vitro methods.

IgE-antibody analysis is a major diagnostic procedure and a primary tool in allergological research. The determination of sensitization frequencies and antibody concentrations against allergens of defined sources provides critical information for the estimation of the relative importance of food and environment in clinical allergy. True quantitation is essential and requires assay designs providing allergen excess and mass unit calibration. Standardized and reproducible methods show geographic and culture dependent differences between patient populations and contribute to the quality of diagnosis and treatment of allergic disease.

Allergens↗
PubMed1997

Oral allergy syndrome to a jackfruit (Artocarpus integrifolia).

A 30-year-old man from the Philippines with pollen allergy noted the appearance of oral allergy syndrome (OAS) after eating raw apple, raw peach, raw celery, and recently, jackfruit (Artocarpus integrifolia), a tropical fruit which belongs to the Moraceae family (mulberry) and to the genus Artocarpus (breadfruit tree). Despite the patient's multiple sensitization in skin prick tests and in the Pharmacia CAP System to birch, grass, mugwort pollen, related fruits and vegetables, and jackfruit, in RAST-inhibition studies neither rBet v 1 nor rBet v 2 (profilin), the well-known cross-reacting allergenic components in OAS, could inhibit the specific IgE response to jackfruit. Whether the reaction to jackfruit is specific or whether other pollen-related, cross-reacting allergenic components exist should be investigated further.

Adult↗
PubMed1996

Inhalant allergy to fresh asparagus.

BACKGROUND: Two patients experienced itching conjunctivitis, running nose, tightness of the throat and coughing during preparation of fresh asparagus. Eating asparagus after cooking did not provoke any allergic symptoms. Both patients were atopic, sensitized additionally to pollens of grasses and trees as well as to onion. OBJECTIVE: To assess the hypersensitivity reactions to fresh and heated asparagus and to investigate any crossreactivities among the allergens. METHODS: Skin-prick tests were performed with commercial allergens and native asparagus and the patients were tested with Pharmacia CAP system for specific IgE antibodies against asparagus, onion, garlic, birch pollen, mugwort pollen and two recombinant birch pollen allergens, Bet v I and profilin. Inhibition of IgE antibody binding to solid phase homologous and unrelated allergens by increasing doses of liquid allergens (inhibitors) was studied. RESULTS: Skin-prick tests with native green and white asparagus were strongly positive, but negative with cooked asparagus. Both patients had measurable levels of IgE antibodies against asparagus (3.0 and 6.2 kU/L respectively) and several other allergens. One patient was highly sensitive to birch and Bet v I. Both were positive to profilin, mugwort and onion. In all cases the antibody uptake could be extensively and specifically inhibited by homologous allergen. The asparagus-specific IgE antibodies of the two patients could only be inhibited by asparagus. No inhibition was obtained after heating of the asparagus extract to 100 degrees C. CONCLUSIONS: The patients were specifically sensitized by asparagus. No immunological crossreactions could be observed. The measurements of IgE antibodies to other allergens were also specific, representing parallel multiple sensitivity. Profilin inhibited profilin-specific IgE binding but did not react with the asparagus-specific IgE antibodies of these patients. The asparagus allergen recognized by the specific IgE antibodies of these patients was thermolabile.

Female↗
PubMed1987

[New diagnostic approaches in immuno-allergology].

Methods for the diagnosis of allergy have shown considerable development in the 1980s. Older tests have been perfected (RAST) or their interpretation improved (total IgE). New tests have appeared for respiratory allergy (Phadiatop) and to follow the course of treatment (IgG RAST). Other tests are in the course of development, for example for the diagnosis of coeliac disease (gluten-specific IgA). In the future we can expect more application of specific antibody measurement and routine use of sophisticated procedures such as immunoblotting.

Humans↗
PubMed1987

[Determination of specific IgE against 16 widespread mould genera. Improvement of the efficacy of the diagnosis of allergy to moulds].

Serum samples from 55 Scandinavian and US patients with a clinical history of mould allergy were screened for specific IgE Ab against 16 different moulds, the 6 mould in the Phadebas RAST panel and 10 new moulds. The studies were performed using RAST-based techniques and the nitrocellulose immunoblotting method. The RAST screening of the patient panel, revealed that 42 patients (76%) had specific IgE to at least one of the 16 moulds. The two most frequent moulds were Rhizopus and Botrytis positive in 29 patients (53%) and 27 patients (42%), respectively. Top three in terms of frequency in the US patient group were: Rhizopus greater than Botrytis greater than Phoma and in the Scandinavian group: Cladosporium greater than Botrytis greater than Helminthosporium. 15 patients (27%) were negative against the moulds in the Phadebas RAST panel, but had specific IgE against one or more of the 10 new moulds. IgE Ab concentrations measured with different genera showed different degrees of positive concordance, e.g. Botrytis-Helminthosporium (79%) and Alternaria-Rhizopus (38%), indicating complex patterns of crossreacting and genus specific allergens. This was confirmed by immunoblotting with 22 (greater than 1 PRU/ml) of the 55 sera showing up to 25 IgE binding components. The results strongly suggest that the importance of some mould genera has been underestimated as allergens.

Antibody Specificity↗
PubMed1985

Antibodies in allergy and inflammation. Research tools and indicators of disease.

The identification of IgE and the development of the radioallergosorbent test was the initiation of a revolution in the allergy research field. Measurement of allergen specific IgE antibodies is today an integral part of the clinical investigation of patients with suspected allergic disease. However, in addition to a being a routine test for the clinical laboratory, Phadebas RAST has served as the model for a number of modified procedures and as the basis for a wide range of applications. The aim of this presentation is to review and communicate the experience for the benefit of researchers and clinicians working in the field of allergy.

Adult↗
PubMed1981

Clinical accuracy of updated version of the Phadebas RAST test.

The clinical sensitivity and specificity of an updated version of the Phadebas RAST test were superior to that of the conventional Phadebas system. In comparison to a three-hour first incubation, an overnight first stage incubation did not contribute to an improvement in the over-all clinical effectiveness of the test system. However, the prolonged first stage incubation did contribute to a better resolution between the normal, non-atopic control group and the atopic patient population.

Alternaria↗
PubMed1980

Dog dander allergens. Specificity studies based on the radioallergosorbent technique.

Cat allergen-specific serum IgE antibodies were detected in 27 (71%) of a group of 38 dog dander-sensitive patients. In 4 (44%) of the cases the dual reaction could be explained by the presence of IgE antibodies to cross-reacting serum proteins. In a larger group the binding of dog dander-specific IgE antibodies could be inhibited by cat epithelium allergens. Two allergenic components of dog dander were separated by chromatography on Sephadex G-75. One of the allergens was closely related to a purified major cat epithelium allergen. The other allergen was apparently dog-specific and showed no cross-reactivity with cat allergens. Both components were present in the dander of dachshund, Airedale terrier, poodle and boxer. The dog dander is the preferable source of dog epithelium allergens provided that the existence of relations to other animals is considered.

Allergens↗
PubMed1979

IgE antibodies to bee venom, phospholipase A, melittin and wasp venom.

Specific IgE antibodies against bee venom, phospholipase A, melittin and wasp venom have been examined in fifty patients with an unusually severe reaction after bee or wasp sting. Two thirds of the bee venom-sensitive patients also have detectable IgE antibodies to wasp venom. More than 50% of the wasp venom-sensitive patients are also allergic to bee venom. Phospholipase A and melittin IgE antibodies were found, respectively, in two thirds and one third of the bee venom-sensitive cases. Specific IgE antibody determinations by the Radioallergosorbent test play an essential role in the diagnostic work. After a reaction to hymenoptera stings both bee and wasp venom tests are necessary due to the high incidence of a false or incomplete identification of the stinging insect. Melittin, known for its potent pharmacological activity and possibly responsible for most of the side effects in bee venom immunotherapy, can probably not be excluded from therapeutic venom preparations since IgE antibodies to the melittin preparation were detected in one third of the cases.

Bee Venoms↗
PubMed1979

Comparison of skin tests and RAST for the diagnosis of bee sting allergy.

Skin tests and estimation of specific IgE-antibodies by the RAST are evaluated as diagnostic procedures in bee sting hypersensitivity with the followiny extracts. (2) With bee venom results of skin tests and RAST correspond in 82%, with bee wholebody extracts in 68%. (3) Both with skin tests and the RAST with bee venom a good discrimination between patients with bee sting hypersensitivity and non-allergic controls is observed. Skin tests with bee venom are somewhat more sensitive than bee venom-RAST. (4) With the careful skin test procedure chosen, no adverse reactions were observed.

Allergens↗
PubMed1978

Use of RAST technique in wasp sting hypersensitivity. Cross-reactions between various insect antigens are specially considered.

Clinical hypersensitivity to wasp stings was found to be fairly well correlated with the presence of serum IgE-antibodies against yellow jacket venom as detected by the RAST technique. Such antibodies were never found in a control group of non-allergic blood donors, but they were detected in a surprisingly large proportion of patients with bee sting allergy without known allergic reactions to wasps. Studies using RAST inhibition technique failed to prove cross-reactions between bee and wasp venoms. Considerably better results were obtained when venom antigens instead of whole body antigens were used in the RAST. RAST inhibition studies suggested that IgE-antibodies detected with RAST using whole body antigen are directed against bee venom constituents in the whole body extract.

Antibodies↗