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Biomedical subjects

L Yang

Publications and source records attributed to L Yang.

854 records · Page 48Linked to original sources
PubMedDate not supplied

Microwave-enhanced immunohistochemical staining of a formalin-sensitive antigen-laminin receptor.

Many potentially useful antigens have been difficult to detect in formalin-fixed, paraffin-embedded tissues. Recently a number of pathological and research laboratories have demonstrated that some antigens masked by formalin fixation could be restored to detectability by microwave heating. Previously, we were unable to demonstrate laminin receptor in cells processed by the routine fixation. Our results showed that microwave heating together with trypsin produced the best immunohistochemical staining for this receptor. Nevertheless, no significance was found in the levels of 67 kD LR in high and low metastatic tumor cell lines.

Adenocarcinoma↗
PubMedDate not supplied

Transjugular intrahepatic portosystemic shunts: midterm results in 28 patients.

This paper reports the clinical and procedural results of 28 consecutive TIPS procedures in 25 males and three females with a mean age of 48 years. All patients had cirrhosis with portal hypertension and varices. Twenty-two patients had recurrent bleeding. Shunts were completed in 26 of 28 patients, and no death was associated with the procedure. Portal vein pressure was reduced from 3.98 +/- 0.24 KPa before shunting to 2.40 +/- 0.16 KPa after shunting. Doppler US revealed that the maximum blood flow velocity in the main portal vein increased from 14.0 +/- 4.5 cm/sec to 48.0 +/- 16.5 cm/sec. Shunt patency was determined by color Doppler US in 20 patients. Shunt stenosis was found in five patients and occlusion in one, and these findings were confirmed by angiography. Ascites disappeared in six of eight cases, and varices disappeared completely in 11 patients and abated greatly in 12 patients two months after TIPS. Rebleeding occurred in three cases (occlusion, 1; stenosis, 2) during a mean follow-up time of 6.5 months. One of these cases was successfully redilated. The initial results suggest that TIPS is a safe and effective method of portal decompression and that the key to portal vein puncture is to understand the three-dimensional relationship between the hepatic and portal veins.

Adult↗
PubMedDate not supplied

In-line leukocyte filtration during bypass. Clinical results from a randomized prospective trial.

Leukocyte mediated pulmonary injury may delay recovery after cardiac surgery, and leukocyte depletion during bypass has been suggested. Two groups of patients were randomly, prospectively assigned from 50 sequential patients to undergo open heart surgery using cardiopulmonary bypass, either with (n = 25) or without (n = 25) leukocyte filters. The two groups were not significantly different regarding age, gender, race, pre-operative ejection fraction, pump time, or cross-clamp time. Post operative arterial blood gases (pO2: 173 +/- 66 vs 192 +/- 107; pCO2: 30.2 +/- 8.2 vs 30.8 +/- 8.0), pulmonary vascular resistance (PVR 105 +/- 45 vs 112 +/- 50 dyne cm-5), time on ventilator (17.8 +/- 6.4 vs 19.7 +/- 8.6 hr), and length of hospital stay (7.65 +/- 4.57 vs 8.52 +/- 5.87 days) were not different between groups (mean +/- SD, with vs without filters, respectively). Arterial oxygenation was somewhat poorer, and PVR was somewhat lower in the leukocyte filtered group. However, these trends did not produce significant decreases in total ventilator time or length of hospital stay. In-line filtration did remove leukocytes, but did not reduce circulating leukocyte count. In effect, leukocyte filtration produced an effective leukocyte concentration at the filter site. These data do not support routine incorporation of in-line leukocyte filtration during bypass.

Cardiac Surgical Procedures↗
PubMedDate not supplied

The association of HPV 16 DNA with specific nuclear matrix proteins of normal and cervical carcinoma cell.

Human papillomavirus type 16 (HPV 16) has been found to be integrated into the DNA of epithelial cells in most cervical cancers. The HPV16 DNA is bound to different nuclear matrix proteins in normal and cervical carcinoma cells. It has high affinity, for acidic proteins in cancer cells. The molecular weights of the acidic proteins are 200 kD, 186 kD and 67 kD. On the other hand, the viral DNA seemed to bind to higher molecular weight basic nuclear matrix proteins (250 kD, 150 kD) of normal cells. Further investigation of the functional roles of these nuclear matrix proteins may provide insight into the process of carcinogenesis of the cervix.

DNA, Viral↗
PubMedDate not supplied

Expression of nm23-H1 in human meningioma cells.

The expression of nm23-H1 has been demonstrated to be highly correlated with the metastatic potential of various tumors. In the present investigation, meningiomas of different pathological grades were used to study on their nm23-H1 expression. Immunohistochemistry showed that nm23-H1 was expressed mainly in the cytoplasm especially in the perinuclear region in explants under short-term culture. Western-blotting demonstrated the specific expression of nm23 protein in all tumor samples. The expression was also found to be sex-dependent on tumor progression in female, but not in male patients. RT-PCR results confirmed nm23-H1 expression was higher in benign tumors than in their normal counterpart. Our observations thus suggest that nm23-H1 may play an important role in the progression of meningiomas in female patients.

Adult↗
PubMedDate not supplied

Electrophoretic analysis of nuclear matrix proteins in human hepatocellular carcinoma.

The nuclear matrix is the non-chromatin skeleton of the nucleus. This structure contributes to the shape of the nucleus and regulates various nuclear functions. In this study, nuclear matrix proteins of human normal liver, a liver cancer cell line, HepG2, and hepatocellular carcinomas (HCC) were investigated. Using high resolution two-dimensional polyacrylamide gel electrophoresis, the nuclear matrix proteins of 3 normal liver and 14 HCC were compared and contrasted. A high degree of similarity between normal liver, HepG2, and HCC nuclear matrix protein patterns was found. Two HCC specific nuclear matrix proteins were identified. Among these, one protein (HCC-1, Mr 62 kd, pI 5.3) appeared in all tumor samples and HCC-2 (Mr 33.25, pI 5.3-5.5) was present in 9/11 tumors, but absent in normal liver and HepG2. Our results indicate the presence of HCC specific nuclear matrix proteins. These matrix proteins may be used as markers for HCC.

Antigens, Nuclear↗
PubMedDate not supplied

Nuclear matrix of human ovarian cancer cells in vitro.

The epithelial neoplasia constitute 60% of all primary tumors of the ovary and 90% of these are malignant. Nuclear matrix has been found to be involved in normal and abnormal nuclear activities. Previously, we have identified tumor-associated nuclear matrix proteins in cancers of human liver, nasopharynx and cervix. In this study, we compared nuclear matrices of immortalized ovarian and cancer cell lines by morphometric and 2-D gel electrophoresis analysis.

Electrophoresis, Gel, Two-Dimensional↗