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Biomedical subjects

L Wu

Publications and source records attributed to L Wu.

At least 757 records · Page 42Linked to original sources
PubMed1990

The proportion of various types of congenital color vision defects.

One hundred and three color vision defective subjects were screened from 3456 middle school students with pseudoisochromatic plate test. One hundred subjects out of them were further examined with a test battery including Panel D-15 and FM 100-hue test and Neitz anomaloscope test. It was found that there were 21 protanopes (P), 3 extremely protanomalous (EPA), 13 protanomalous (PA), 25 deutenopes (D), 5 extremely deuteranomalous (EDA), 28 deuteranomalous (DA), 6 unclassified subjects in our investigation. The proportion of various types of congenital color vision defects was P:EPA:PA:D:EDA:DA = 1.00:0.14:0.62:1.19:0.24:1.33.

Adolescent↗
PubMed1990

[Visual pigment genes for color vision defects].

Applying recombinant DNA techniques, the structures of red pigment gene (RPG) and green pigment gene (GPG) were analyzed for 43 patients with protan or deutan (including 3 females), 4 normal relatives and 3 carriers out of 3 families, as well as 11 normal controls. Abnormality of RPG was detected in all 19 protan and that of GPG was found in 14 out of 24 deutan. In about 80% (32/40) of protan and deutan the changing of exon 5 for RPG or GPG was discovered. In protan the normal RPG was replaced by a 5' red -3' green hybrid gene. Some of the deutan had no GPG, some had 5' green -3' red hybrid gene with or without GPG. Furthermore, the exon 5 of RPG and GPG was amplified by polymerase chain reaction (PCR) and further analyzed by Rsa I digestion. The results for PCR are identical to that of Southern blot hybridization.

Chromosome Deletion↗
PubMed1990

[The visual-vestibular interaction in normal subjects].

Full-field optokinetic nystagmus (OKN) at constant speed of 40 0/s and 60 0/s, vestibular ocular reflex (VOR) (sinusoidal 0.2 Hz, 60 0/s peak velocity) with different background (light and dark) and VOR-fix were observed in 72 normal subjects (144 normal eyes). The gain, FCV and DP of OKN, VVOR, VOR were also analysed. The gain of OKN is reduced accompanied with stimulation increasing, while the FCV is increased. The gain of VVOR is 1.08 +/- 0.09 (X +/- SD), while the gain of VOR is 0.64 +/- 0.09. The FCV of VOR is much reduced than that of VVOR. The FCV in the groups under 30 years of age are much faster than the groups above 30 years of age. The VOR--fix gain is 0.05 +/- 0.04. The DP of OKN, VVOR, and VOR are 0.05 +/- 0.04. The results showed an co-operation between visual and vestibular systems and the results also suggested that the series methods of visual-vestibular interaction (OKN, VVOR, VOR, VOR-fix) might be useful in eye movement examination.

Adolescent↗
PubMed1990

[Effects of total saponins of Panax notoginseng on increasing PGI2 in carotid artery and decreasing TXA2 in blood platelets].

Total saponins of Panax notoginseng (PNS) were given orally 100 mg/(kg.d) to rabbit for 8 wk. Aortic atherosclerotic plaque formation was restrained as compared to the control group. Radioimmunoassay was used to investigate the effects of PNS on the contents of prostacyclin in carotid artery and thromboxane A2 in blood platelets of rat. Oral administration of PNS 25,50,100 mg/(kg.d) for 10 d, the caused an increase of prostacyclin in carotid artery and a decrease of thromboxane A2 in blood platelets as compared to the control group. These results show that the anti-atherosclerotic action of PNS may be a result of the correction of the unbalance between prostacyclin and thromboxane A2.

Animals↗
PubMed1990

[Studies of rapid detection of drug-resistance of Mycobacterium by gas chromatography].

This test is based on the physiologic character of mycobacterium, which produces carbon dioxide (CO2) during its growth, Löwenstein-Jensen media CO2 contents-were measured by gas chromatography for determining drug-resistance of mycobacteria. This was compared with the routine method. Seventy-eight mycobacteria were isolated from suspected tuberculosis patients for the experiment. Our data suggested that the accordance rates of both methods were 83.3%. Gas chromatography is a simple, rapid and accurate method for detecting drug-resistance. The results can be obtained twenty days earlier than by the routine method.

Antitubercular Agents↗
PubMed1990

[Visual pigment genes for color vision defects].

Applying recombinant DNA techniques, the structures of red pigment gene (RPG) and green pigment gene (GPG) were analyzed for 43 patients with protan or deutan (including 3 females), 4 normal relatives and 3 carriers out of 3 families, as well as 11 normal controls. Abnormality of RPG was detected in all 19 protan and that of GPG was found in 14 out of 24 deutan. In about 80% (32/40) of protan and deutan the changing of exon 5 for RPG or GPG was discovered. In protan the normal RPG was replaced by a 5' red -3' green hybrid gene. Some of the deutan had no GPG, some had 5' green -3' red hybrid gene with or without GPG. Furthermore, the exon 5 of RPG and GPG was amplified by polymerase chain reaction (PCR) and further analyzed by Rsa I digestion. The results for PCR are identical to that of Southern blot hybridization.

Color Perception↗
PubMed1990

[The visual-vestibular interaction in normal subjects].

Full-field optokinetic nystagmus (OKN) at constant speed of 40 degrees/s and 60 degrees/s, vestibular ocular reflex (VOR) (sinusoidal 0.2Hz, 60 degrees/s peak velocity) with different background (light and dark) and VOR-fix were observed in 72 normal subjects (144 normal eyes). The gain, FCV and DP of OKN, VVOR, VOR were also analysed. The gain of OKN is reduced accompanied with stimulation increasing, while the FCV is increased. The gain of VVOR is 1.08 +/- 0.09(mean +/- SD), while the gain of VOR is 0.64 +/- 0.09. The FCV of VOR is much reduced than that of VVOR. The FCV in the groups under 30 years of age are much faster than the groups above 30 years of age. The VOR-fix gain is 0.05 +/- 0.04. The DP of OKN, VVOR, and VOR are 0.05 +/- 0.04. The results showed an co-operation between visual and vestibular systems and the results also suggested that the series methods of visual-vestibular interaction (OKN, VVOR, VOR, VOR-fix) might be useful in eye movement examination.

Adolescent↗
PubMed1990

[The visual evoked potentials in ischemic optic neuropathy].

In this study the pattern-reversal stimulation was used for analysing the clinical VEP in 27 cases (37 eyes) of ischemic optic neuropathy (ION), and combined uses of visual field (VF) and contrast sensitivity (CS) examinations were compared and observed with 3 kinds of spatial frequencies (60', 30', 15' check size), VEP in the eyes of ION displayed decrease in amplitude as a dominant manifestation accompanied by the slight increase of latency period. The abnormal latency and amplitude were 83.5% and 81.3% respectively. The decreased CS in the ION eyes was appeared mainly in the stimulation of middle and high frequencies or all frequency bands. There was a significant correlation between the VEP amplitude and CS in the patients with ION. The quadrant VF defect and bow-shaped scotoma were displayed in majority of the cases. Abnormal VEP was shown to be related to the central VF impaired. Among these patients, 7 cases (8 eyes) were followed up with VEP, however the VEP amplitude was shown decreasing somewhat compared with first exam. The results indicated that the ION was responsible for many visual function damages.

Adult↗
PubMed1990

Studies of neuron-specific enolase levels in serum and cerebrospinal fluid of children with neurological diseases.

Neuron-specific enolase (NSE) has recently proved to be a useful marker of neuron damage. We determined NSE levels in the serum and CSF of 117 children with various neurological disorders (43 with febrile convulsion, 25 with seizure disorder, 32 with meningitis, 3 with brain tumor, 2 with Reye syndrome, 3 with congenital CNS malformation and 9 with other disorders). The purpose of this study is to assess the potential usefulness of NSE in diagnosis and prognosis. Twenty CSF and serum samples of children without neurological problem served as a control. The mean values of the NSE levels in the CSF and serum of the control group were 5.00 +/- 1.65 ng/ml and 8.34 +/- 4.40 ng/ml respectively. The peak values were found in cases with brain tumor. A patient died of Reye syndrome didn't show a very high level of NSE in the serum or CSF. However, we found significant differences in NSE levels between the patients with febrile convulsions and those with seizure disorders (non-febrile, abnormal EEG). Most of our patients with febrile convulsions were cases of simple febrile convulsion, and their NSE levesin the CSF and serum were 4.55 +/- 1.00 and 8.06 +/- 3.18 ng/ml. Cases with non-febrile seizure disorders had significantly higher level of NSE in both CSF and serum (P less than 0.05). Patients with purulent meningitis usually had higher levels than those with aseptic meningitis. Our study can be summarized thus: 1. A normal level of NSE does not exclude severe neuron damage.(ABSTRACT TRUNCATED AT 250 WORDS)

Brain↗
PubMed1990

[Mosquito-net spraying with deltamethrin for malaria control].

Mosquito-nets were sprayed with 9.6mg deltamethrin per square meter for control of malaria vectors in Yibin County, Sichuan Province in 1986. In the study area, the relative population indices of Anopheles anthropophagus and An. sinensis in 35 mosquito-nets were 0.6 and 1.4 respectively; the man biting rates of these two vectors dropped by 97.8 and 90.8% respectively. The residual toxic effect of sprayed mosquito-nets, up to 104 days after spray, caused a mortality of 93.1-100% of An. anthropophagus and 87.1-100% of An. sinensis. The multiparous mosquito rate dropped by 48.4 and 51.3%. Indoor-intruding An. anthropophagus and An. sinensis were reduced by 76% and 78.3% respectively. The incidence of malaria decreased by 94.4%, the positive rate in school children declined by 77.5%. Both entomological and epidemiological indices indicated that mosquito-nets sprayed with deltamethrin could effectively control the malaria prevalence. This measure is economical and practical for control of malaria.

Animals↗
PubMed1990

[Diagnostic value of amniotic fluid alkaline ribonuclease activity in anencephaly and IUGR].

Amniotic fluid and maternal serum alkaline ribonuclease (RNase) activities were measured in women with normal pregnancy, fetus, neural tube defect, and fetal intrauterine growth retardation (IUGR). Amniotic fluid alkaline RNase activity was high in anencephaly and IUGR. The mechanism of these changes and the clinical application of these findings are discussed.

Amniotic Fluid↗
PubMed1990

[Study on change in zinc concentration for diabetic cataractous lenses in rats by means of XIXA].

The SD rats were injected with streptozotocin to induce diabetic cataract. Element Zn as well as K, Ca, Mn, Fe, Cu, Pb, Se, etc. in the eye lenses was determined by means of X-ray induced X-fluorescence analysis (XIXA). Compared to values for normal healthy rats, the concentration of Zn and Fe in the cataractous lenses increased, the concentration of K decreased, and that of the others did not change prominently. The change in Zn concentration was probably to meet the needs of the metabolic adjustment in the eye lenses, which would not be the reason for directly inducing diabetic cataract.

Animals↗
PubMed1989

A dihydropyridine-sensitive calcium channel in rodent osteoblastic cells.

Rat osteogenic sarcoma cells (UMR 106-01) and normal rat trabecular bone osteoblasts (ROB) were studied using the whole cell version of the patch clamp technique to determine the existence of calcium (Ca2+) channels. Pipette and bath solutions were designed to separate Ca2+ channel currents from other voltage-dependent currents, and Ba2+ was used as the charge carrier. In both UMR 106-01 and ROB cells, a Ba2+ current was measured, which expressed the characteristics of an L-channel, such as activation range, dihydropyridine sensitivity, and little or no inactivation. In some cases, this channel was detectable only with BAY-K-8644 in the bath solution. The dihydropyridine agonist increased the current intensity and shifted the peak inward current to more negative potentials. This study, confirming previous observations, demonstrates the existence of a Ca2+ channel in both transformed and normal osteoblastic cells.

Animals↗
PubMed1989

A murine early thymocyte developmental sequence is marked by transient expression of the interleukin 2 receptor.

Precursors of all T-lineage cells are found in the population of thymocytes that lacks the CD4 and CD8 surface markers. These "double-negative" thymocytes are heterogeneous and can be divided into discrete subpopulations based on their expression of other surface markers. We have determined the relative maturity of these subpopulations based on the extent of rearrangement and expression of their T-cell receptor genes, their cell cycle status, and their thymus reconstitution capacity. Within the subpopulation of double negatives expressing high levels of the heat-stable antigen, the additional markers phagocytic glycoprotein 1 (Pgp-1) and interleukin 2 receptor (IL-2R) can be used to define the sequence IL-2R- Pgp-1+----IL-2R+ Pgp-1-----IL-2R- Pgp-1-, which occurs before the expression of CD4 and CD8. Transient expression of the IL-2R marks an important developmental point in the sequence just prior to a burst of cell proliferation and a loss of thymus reconstitution ability. The earliest cells in this sequence are already partially rearranged for genes in the C beta 1 region. IL-2R expression marks a second wave of T-cell antigen receptor of beta-chain gene rearrangement and the initiation of T-cell antigen receptor alpha- and beta-chain gene expression.

Animals↗
PubMed1989

Sex determination of Chinese femur by discriminant function.

The objective of this study was to provide a method of sex determination for Chinese femur, especially for fragmentary remains. Statistical analysis of 17 measurements based on 141 northeastern Chinese femora shows that all measurements have significant sex differences. Sex determination by the midpoint method of a single measurement can obtain an average accuracy of 76.8%. The results indicate that of all dimensions the maximum head diameter is the best discriminator of sex. It alone can determine the sex of a femur with 85.1% accuracy. The results also show that maximum head diameter, transverse head diameter, epicondylar breadth, upper breadth, and medial condylar length have higher sexual dimorphisms than the dimensions of length. According to the result to stepwise discriminant analysis and the need for sexing fragmentary remains, 22 discriminant functions composed of various combinations of variables were calculated which can determine the sex of the femur correctly 82.3 to 87.2%. The methods of sex determination provided by this paper can be used to sex poorly preserved femora.

Asian People↗