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Biomedical subjects

L Wu

Publications and source records attributed to L Wu.

At least 397 records · Page 22Linked to original sources
PubMed1998

[A study on the distribution and etiology of pathogenic vibrio in the 5 coastal provinces of China].

In order to understand the distribution and pathogenicity of vibrio in the coastal areas of China a study consisted of 51 water samples and foodstuff from 10 different areas in 5 provinces was conducted microbiologically and etiologically. Results showed that 45 pathogenic vibrio were identified, with majority of A. caviae (26.7%), V. alginolyticus (22.2%) and V. parahaemolyticus (11.1%), same kinds of vibrio found in feces from patients with acute infectious diarrhea. Prevalence rates of pathogenic vibrio identified from the East China Sea, the South China Sea and the Yellow Sea were between 66%-77%. The detection rate of pathogenic vibrio in 29 water samples was 93.1% (27/29) with 38 strains isolated mainly in river water (57.8%), followed by in sea water (21.1%) and pool water (21.1%). The results revealed that the distribution of pathogenic vibrio in different waters was closely related to its pathogenecity, providing evidence that vibrio diarrhea be supervised, diagnosed and treated in order to decrease the incidence of the disease.

China↗
PubMed1998

[The clinical value of tissue polypeptide antigen in ovarian carcinoma].

OBJECTIVE: To evaluate the clinical usefulness of tissue polypeptide antigen (TPA) in diagnosis and monitoring the course of patients with ovarian carcinoma. METHODS: Serum levels of TPA and cancer antigen 125 (CA125)were measured by radioimmunoassay in 60 patients with advanced ovarian carcinoma (41 with active disease and 16 with nonactive, 3 with metastatic tumor from gastrointestinal carcinoma), 24 healthy women and 27 benign gynecologic tumors. RESULTS: The serum TPA and CA125 levels were elevated in 82% and 70% of patients with ovarian carcinoma respectively, the total positive rate of the two markers was 92%. TPA and CA125 levels were within normal range among most of healthy women and patients with benign tumors. TPA and CA125 levels were also correlated with the regression or progression of the disease. CONCLUSION: Combined measurement of TPA with CA125 was useful for the differential diagnosis and achieving higher positivity in patients with epithelial ovarian carcinoma.

Adult↗
PubMed1998

[Clinical experience with iron supplementation in pregnancy].

OBJECTIVE: To study the efficacy of iron supplementation during pregnancy and its influences on the outcome of pregnancy. METHODS: A total of 369 pregnant women were enrolled in this study. According to the hemoglbin levels at recruitment, there were 2 groups: preventive (Hb > or = 110 g/L) and treatment (Hb < 110 g/L) groups. In the preventive group, women entered the study from 20-24 gestational weeks and were randomly assigned to materna treatment (n = 96) who took materna 1 tablet daily or control group (n = 95) who took no other supplementation. In the treatment groups, women less than 36 gestational weeks were accepted. They were randomly divided as materna (n = 93) 1 tablet/d, ferrous sulfate 0.3 g tid/d (n = 50) or Ferroids 1 tablet/d (n = 35) groups. Both Hb and serum ferritin concentrations were determined at admission and immediately after delivery. In some cases serum ferritin in the umbilical vein were measured as well. Hemoglobin levels were examined every 4 weeks during the observational period. RESULTS: In the preventive groups, maternal serum ferritin levels after materna treatment were significantly higher than that before treatment and the control group (P < 0.05). As for the anemia women, compared the serum ferritin concentration, materna treatment had significantly higher levels than that at admission (P < 0.05), and also higher than that in the ferrous sulfate or ferroids tratment groups (P < 0.05). The s-ferritin in the umbilical vein had no correlation with the paired maternal levels. There were no significant differences in the pregnancy outcomes among all the groups. CONCLUSION: Materna can increase the iron storage and effectively improve the iron deficiency during pregnancy, and has no impact on the prgnancy outcomes.

Adult↗
PubMed1998

[The effect of electroacupuncture stimulation an neurotrophic substance in cat spinal dorsal horn].

For exploration of the mechanism of collateral sprouting from injured spinal cord, polyacrylamide gel electrophoresis (PAGE) and modified hanging drop culture method were used to examine the effect of acupuncture on the amount and the biological activity of neurotrophic substance in larger than 50 kd fraction of dorsal horn in cat subjected to partial dorsal rhizotomy. The results showed that the amount of protein in relative mobility (RM) 0.1 zone increased significantly in the acupuncture-operated group by PAGE and that of protein in RM 0.5 zone decreased significantly as compared with the unoperated group. While this data was compared with the data of another operated group in our lab, the amount of RM 0.1 protein of the acupuncture-operated group was significantly higher than that of the latter group; however, no obvious difference was noted between the two groups in the amount of RM 0.5 protein. It suggests that acupuncture may promote tha amount of RM 0.1 protein to increase, but acupuncture has no influence on the amount of RM 0.5 protein. Also, the results showed that, in culture, accompanying with the increase in amount, the activity of RM 0.1 protein for promoting DRG neurite-outgrowth increased. This indicates that the RM 0.1 protein has a function to promote DRG neurite-outgrowth, but the RM 0.5 protein has no obvious effect on DRG neurite-outgrowth.

Animals↗
PubMed1998

[An immunohistochemical study of the proliferating activity of ameloblastic fibroma and ameloblastic fibrosarcoma].

Six cases of ameloblastic fibroma (AF) and three cases of ameloblastic fibrosarcoma (AFS) were immunohistochemically investigated with a proliferating cell nuclear antigen (PCNA) monoclone antibody. Data on the PCNA labeling index (LI) of both epithelial and mesenchymal components were analyzed. The results showed that the PCNA LI of mesenchymal component of AFS (40.8%) was significantly higher than that of AF (3.2%) (P < 0.01) and that of the epithelial component of AFS (5.3%) was significantly lower than that of the mesenchymal one of AFS (P < 0.01). The results suggested that PCNA LI be a useful marker for the differentiation diagnosis of AF and AFS.

Cell Division↗
PubMed1998

[Different types of acidemia at birth, fetal heart rate patterns and infants outcome at four years of age].

OBJECTIVE: To study the relations between different types of acidemia in umbilical artery at birth, fetal heart rate patter'ns (FHR) during laobr and infant long-term outcome. METHODS: 2,981 cases with singleton term pregnancy were selected for retrospective case-control study. Fetal monitoring was performed periodically during labor and umbilical blood gas was analyzed at birth and the infants outcome was followed up at age 4. RESULTS: There were 47 infants with pure metabolic acidemia and 67 infants with mixed acidemia. The incidence of FHR score < 6 and 6-7 at the end of first stage was significantly higher in the two study groups than that in the control group. Late deceleration was associated with pure metabolic acidemia and complicated variable deceleration with mixed acidemia. Both types of acidemia were correlated with Apgar scores below 7 at 1 minute after birth. Developmental screening at age 4 showed no significant differences between the two study groups and the control groups. In the mixed acidemia group there were slightly more infants had deficits in speech/language development. CONCLUSIONS: Late deceleration may be an indicator of pure metabolic acidemia and complicated variable deceleration be an indicator of mixed acidemia. Both types of acidemia were correlated with Apgar scores below 7 at 1 minute after birth. The relation between acidemia at birth and deficit in speech/language development should be further evaluated.

Acidosis↗
PubMed1998

Mitochondrial gene defect in patients with chronic progressive external ophthalmoplegia.

OBJECTIVE: To detect the gene defect of mitochondrial DNA (mtDNA) from skeletal muscles in 2 patients with chronic progressive external ophthalmoplegia (CPEO). METHODS: After extraction of mtDNA, Southern hybridization was performed after restrictive digestion by Pvu II, EcoRI, Hind III, and Sacl. Then, we carried out polymerase chain reaction (PCR) and the enzyme digestion of the PCR products. Finally, mtDNA sequencing was done by automatic DNA sequence analyzer. RESULTS: In case 1, a 5 kb deletion was found by Southern blot analysis and PCR. And dosage analysis showed a heteroplasmic change with 44% mtDNAs deleted. In case 2, PCR plus restriction endonuclease Pvu II digestion demonstrated a mutation which was confirmed by DNA sequencing to be a single base substitution (T-->C) inducing a novel Pvu II site around 10,909 on mtDNA sequence. The laser image analyzer measurement revealed the mutation was almost homologous (99.4% mutant). CONCLUSIONS: In case 1, a 5 kb deletion found in mtDNA is called "common deletion" according to the literature. In case 2, a novel Pvu II site was found. It seems to be a de novo point mutation affecting ND4 in published CPEO research and is first reported in Chinese population. This point mutation does not induce an amino acid(Phe) change according to the published human mitochondrial genetic code as well as the mtDNA sequence. Whether it affects the translation efficiency or transportation of signals between mitochondrial and nuclear genome needs further studies.

Adult↗
PubMed1998

[The surgical management of the carotid artery involved with the head and neck cancer].

To discuss the possibility of resection of the carotid artery involved with head and neck cancer without reconstruction. 5 cases of tumor fixed to segment of the carotid artery block were resected, among them 4 without reconstruction and only 1 artery anastomosed. None of them experienced cerebrovascular complications. 3 cases survived two years long, and 2 is now under follow-up. The selective resection of the carotid artery without reconstruction was practical if well prepared.

Aged↗
PubMed1998

[Neuronal activity of monkey dorso-lateral premotor cortex during tasks of figure recognition guided motor sequence vs memorized spatial motor sequence].

In the last 2-3 decades the role of the premotor cortex (PM) of monkey in memorized spatial sequential (MSS) movements has been amply investigated. However, it is as yet not known whether PM participates in the movement sequence behaviour guided by recognition of visual figures (i.e. the figure-recognition sequence, FRS). In the present work three monkeys were trained to perform both FRS and MSS tasks. Postmortem examination showed that 202 cells were in the dorso-lateral premotor cortex. Among 111 cells recorded during the two tasks, more than 50% changed their activity during the cue periods in either task. During the response period, the ratios of cells with changes of firing rate in both FRS and MSS were high and roughly equal to each other, while during the image period, the proportion in the FRS (83.7%) was significantly higher than that in the MSS (66.7%). Comparison of neuronal activities during same motor sequence of two different tasks showed that during the image periods PM neuronal activities were more closely related to the FRS task, while during the cue periods no difference could be found. Analysis of cell responses showed that the neurons with longer latency were much more in MSS than in FRS in either cue or image period. The present results indicate that the premotor cortex participates in FRS motor sequence as well as in MSS and suggest that the dorso-lateral PM represents another subarea in function shared by both FRS and MSS tasks. However, in view of the differences of PM neuronal responses in cue or image periods of FRS and MSS tasks, it seems likely that neural networks involved in FRS and MSS tasks are different.

Animals↗
PubMed1998

[Analysis of heart rate variability in Chinese healthy pilot].

Normal range of 24 h heart rate variability (HRV) and its application in healthy pilots of different age and sex were investigated. 24 h HRV data in 118 healthy male and female pilots and ground crew were measured. Comparing with male pilots, SDNN, HRVi, RMSSD, HF and ATP values in females are obviously higher (P < 0.05). For HRV comparison analysis, the pilots were divided into three age groups. The results show that SDNN value of male pilots in group A is obviously higher than those in group B and C (P < 0.01). In female pilot group, the RMSSD, HF and ATP values of group C, are obviously higher than those of A and B (P < 0.01). It demonstrates that many HRV parameters show significant difference between male and female pilots, the HRV parameters in female pilot are obviously higher than those in males, especially for such parameters which reflect vagal nerve activity. Age factor effect is apparent in pilot's HRV data. Some HRV parameters in different age groups show significant difference. Most 24 h HRV parameters in pilots flying different types of aircraft show no significant difference.

Adult↗
PubMed1998

[Analysis of time domain and frequency domain heart rate variability in fighter pilot before and after upright tilt].

Effects of upright tilt on mechanism of autonomic nervous regulation of cardiovascular system and characteristics of heart rate variability (HRV) were observed in sixty healthy male pilots. Relation between time domain and frequency domain indexes of short-time HRV (5 min) were analysed before and after upright tilt. The results showed that there are significant difference in short time HRV parameters before and after upright tilt. Significant relationship was formed between time domain and frequency domain indexes of HRV. It suggests that time domain and frequency domain HRV analysis is capable of revealing certain informations embedded in a short series of R-R intervals and can help to evaluate the status of autonomic regulation of cardiovascular function in pilots.

Adult↗
PubMed1998

[Verification of a new processing technology for pilot production of Aconitum coreanum (Lévl.) Raipaics].

The stability and feasibility of a new technology for processing Aconitum coreanum have been tested for pilot production by determining the components, toxicity(LD50) and traditional identification standard. The result shows that the contents of guanfu A and total alkaloid between the small trial and pilot production are slightly different and LD50 are nearly similar. Hypaconitine was not found in all kinds of processed products. The new technology has thus been proved stable and feasible.

Alkaloids↗
PubMed1998

[Clinicopathological and diagnostic study of amyloidosis of oral maxillofacial area].

OBJECTIVE: To study the clinicopathological features of amyloidosis studied in oral maxillofacial area and the diagnostic methods. METHODS: HE staining and staining of alkaline Congo red, Immunohistochemical staining, Sections examined by transmitted and polarized light microscopy, Transmitted electron microscopy. RESULTS: 14 cases of the disease were reported. Clinically, the patients usually had progressive enlargement and solidness of the tongue (macroglossia, 12 cases), hard nodules or cords of the cheek and lip mucosa (8 cases), single or multiple hard masses of submandibular and submental areas (6 cases), and ecchymosis or petechiae of the face, neck, chest or oral mucosa. Microscopically, the disease is characterized by the even pink amyloid protein deposit in the soft tissues and the wall of blood vessles, which shows positive staining of alkaline Congo red, Immunohistochemical staining can differentiate the primary amyloidosis from secondary one. Ultrastructurally, Amyloid protein appeares as felt-like finely filament. CONCLUSION: Amyloidosis involving in oral maxillofacial area mainly arise in tongue, less frequently, in buccal and lip mucosa, salivary glands. It is not difficulty to dignosis the disease by HE staining combining with staining of alkaline Congo red and other specific staining methods. Ultrastructural and immunohistochemical study is helpful to make show the dignosis.

Adult↗
PubMed1998

[The testing of local electroretinogram in macular diseases].

OBJECTIVES: To record the local electroretinogram (LERG) in macular diseases, and define the using value of LERG in macular diseases. METHODS: The LERGs of five kinds of maculopathies were recorded with a spot light stimulator at 2.5, 5.0, 7.5 degrees of macular region. RESULTS: (1) The amplitudes of LERG at the three regions in Stargardt's disease, wet form age-related macular degeneration (AMD), central serous chorioretinopathy, and idiopathic macular hole were significantly lowered in comparison with the controls (P < 0.01). In the meanwhile, these diseases also showed high abnormal rates. While in dry form AMD and epiretinal membrane, the amplitudes of LERG had no significant changes (P > 0.05). (2) The abnormal rates of LERG were related to the stimulated area. In the three tested areas, the highest abnormal rates occurred at 2.5 degrees. CONCLUSIONS: There are mean differences in macular electrical activities in these maculopathies. LERG is an effective method of electrophysiology for testing macular function.

Adult↗
PubMed1998

[The fluorescein angiography and indocyanine green angiography of high myopia with macular hemorrhage].

OBJECTIVE: To investigate the causes and fundus changes of high myopia with macular hemorrhage. METHODS: Color photography and fundus fluorescein angiography (FFA) were performed on 35 patients (37 eyes) of high myopia with macular hemorrhages, and indocyanine green angiography (ICGA) was performed on 8 patients. RESULTS: The causes of high myopia with macular hemorrhage could be divided into two forms: (1) macular hemorrhage with subretinal neovascularization (23 eyes), (2) macular hemorrhage without subretinal neovascularization (14 eyes). ICGA noted the neovascularization more well-defined and slightly bigger than FFA. In 9 of 14 eyes, lacquer cracks appeared at the sites of macular hemorrhage or around the hemorrhage. 6 eyes were followed up, the follow-up period ranging from 3 to 20 months (mean, 8.6 months). ICGA was performed on two patients. The results show that the rupture of choriocapillaries and Bruch's membrane can result in hemorrhage without subretinal neovascularization, and ICGA is more sensitive in detecting the lacquer cracks than FFA. CONCLUSION: Subretinal neovascularization and the formation of lacquer cracks result in two forms of high myopia with macular hemorrhage. ICGA combined with FFA is more useful in evaluating the two forms of hemorrhage.

Adolescent↗
PubMed1998

[Scanning electron microscope observation and image quantitative analysis of Hippocampi].

The "scale-like projects" on the derma of 3 species of Hippocampi, H. kuda Bleerer, H. trimaculatus Leach and H. japonicus Kaup were observed by scanning electron microscope (SEM). Results showed that some characteristics such us size, shape and type of arrangement of the "scale-like projects" can be considered as the evidence for microanalysis. Image quantitative analysis of the "scale-like project" was carried out on 45 pieces of photograph using area, long diameter, short diameter and shape factor as parameters. No difference among the different parts of the same species was observed, but significant differences were found among the above 3 species.

Animals↗
PubMed1998

[Chemical stability of salmon calcitonin (sCT) analogues in aqueous solution].

Calcitonin is a peptide hormone with 32 amino acids. Natural calcitonins are presumably quite unstable in solution and numerous analogues of calcitonin had been synthesized to improve the stability. In the present paper, we report the investigation of the chemical stability of salmon calcitonin (sCT) analogues [Val1, Ala7]sCT, [Val1, Ala7, Ala30]sCT and [D-Ala30]sCT in aqueous solutions by reversed phase high-performance liquid chromatography (RP-HPLC) and fast atom bombardment mass spectrometry (FAB-MS) methods. The degradation patterns of the sCT analogues showed a marked dependence on pH and temperature. Most of the degradation products in pH 9.0 solution of sCT and its analogues were identified by FAB-MS analysis. Results showed that the sCT analogues are more stable at pH 3.3 and pH 3.7 than at pH 6.0 and pH 9.0. Analogues without disulfide bridge [Val1, Ala7]sCT and [Val1, Ala7, Ala30]sCT showed higher chemical stability than sCT while [D-Ala30]sCT showed lower chemical stability than the native sCT.

Calcitonin↗