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L Wolska-Goszka

Publications and source records attributed to L Wolska-Goszka.

At least 19 recordsLinked to original sources
PubMed2000

[Measuring of the level of IGG antibodies against mycobacterial A60 antigen in the differential diagnosis of lung tumors].

In some cases it is difficult to define an etiology of lung tumour in the other way than thoracotomy. In these diagnostic difficulties patients with tuberculous tumour are also involved. The aim of the present study was to define if A60-ELISA test, a new valuable tool in the diagnosis of tuberculosis, might be useful in the differential diagnosing of lung tumours. The levels of IgG antibodies against A60 antigen of mycobacteria was measured with the use of the ELISA test. 228 persons were enrolled into the study: 58 healthy controls (Group I), 26 nonmalignant and nontuberculous patients (Group II), 56 patients with the diagnosis of pulmonary tuberculosis bacteriologically confirmed (Group III), 54 pulmonary tuberculosis patients culture negative (Group IV), 16 patients with lung tumour, without histologic diagnosis before thoracotomy (Group V) and 18 patients with defined diagnosis of lung cancer (Group VI). By using 200 U/ml as a cut-off point the test was positive in: 2/58 subjects from Group I, 2/26 from Group II, 44/56 from Group III, 31/54 from Group IV, 3/16 from Group V and 0/18 from Group VI. In three patients from group V having positive result of A60-ELISA test pulmonary tuberculosis was diagnosed when the thoracotomy was performed. We conclude that the new, simple, noninvasive and inexpensive A60-ELISA test migt be the useful tool in the differential diagnosing of lung tumours because of its good sensitivity and specificity in pulmonary tuberculosis. We also consider that in lung tumour patients with the positive result of this test the prophylactic antituberculosis treatment have to be ordered before planned thoracotomy.

Adolescent↗
PubMed1999

[Humoral immune response against A60 antigen from tuberculosis expectoration and the clinical and radiologic state of patients with with pulmonary tuberculosis].

Serological tests available for diagnosis of tuberculosis can provide the valuable informations about host immune response to the mycobacterial infection. The aim of our study was to assess the correlation between clinical, radiological and bacteriological state of pulmonary tuberculosis patients and the serum concentration of IgG antibodies against A60 antigen of Mycobacterium bovis BCG. 110 active pulmonary tuberculosis patients--56 culture positive and 54 culture negative--were involved into the study. In all patients serum level of IgG ati-A60 antibodies was measured with the use of A60-ELISA test. We found that bacteriologically confirmed pulmonary tuberculosis is associated with higher serum level of anti-A60 antibodies. Our study also showed the significantly higher levels of anti-A60 IgG in patients with cavitary and more progressive forms of this disease. We conclude that the greater antigenic challenge in progressive forms of pulmonary tuberculosis stimulates more expressed antibody response.

Adult↗
PubMed1999

[Cefotaxime in sequential therapy of community acquired pneumonia].

Recent studies indicate clinical value of intra-venous to oral switch therapy. The aim of this study was to analyze our results i.v. Cefotaxime to oral Cefetamet Piroxil switch therapy in lower respiratory tract infections. Group consists 35 patients in whom 27 has bacterial pathogen definitely established. Cefotaxim (Tarcefoksym-Polfa) i.v. has been used for 3-4 days followed with oral Cefetamet Pivoxil (Tarcevis-Polfa). Patients has been treated for 7 days in hospital and then as an out-patients afterwards. Excelent result has been observed in 27 cases (77% of the study group). Mild symptoms of medication intolerance has been observed in 8 patients (23% of all patients). Results of our experience with third generation cefalosporin i.v. to oral switch therapy are satisfactory. This method reduced total cost of treatment, reduced in-hospital days and was very well accepted by patients.

Adult↗
PubMed1999

[Mycoplasma pneumonia in own clinical material].

UNLABELLED: The majority of community acquired pneumonia patients are treated by family doctors, most frequently in empiric way, what delays the treatment when a choice of antibiotic is not proper. In this case the resistance to the antibiotics of the bacteria can occurred and there is more often the necessity of patient admission to the hospital. The aim of the study was to analyse retrospectively the treatment of mycoplasmal pneumonia patients. The material consisted of consecutive 27 patients (17 females and 10 males; average age 31.9 yrs.) treated in the Pneumonology Department of Medical University of Gdańsk in the period from 1995 to 1997 year because of mycoplasmal pneumonia. The etiologic diagnosis was defined first of all on the base of serologic test, measuring the complement-fixing antibodies, beside clinical data. The retrospective analysis revealed that all patients had been admitted to the hospital after previous not effective anti-bacterial or anti-viral treatment as out-patient. At the admission no patients had the temperature exceeding 38 degrees C, myalgia and headache occurred in 44% and 40.7% of patients respectively, splenomegaly was reported in 4 patients. In chest roentgenograms pulmonary infiltrations were observed in all patients, most frequently unilaterally (68% of pts.), in 29% of patients complicated by pleural effusion. The titers of complement-fixing antibodies against Mycoplasma pneumoniae antigen ranged from 1:245 to 1:2000. In the treatment most frequently (70.3% of cases) doxycycline in monotherapy was used. Eight patients were treated with doxycycline together with erythromycine. In 33.3% patients the antimycoplasmal treatment was ordered only on the base of clinical data. The period of hospitalisation ranged from 18 to 34 days, on an average 24 days. CONCLUSION: In case of mild community acquired pneumonias with not defined ethiology, if the initial antibacterial or antiviral treatment is not effective, tetracyclines or/and erythromycine should be ordered without the necessity to confirm the diagnosis of mycoplasamal pneumoniae with the serological test.

Adolescent↗
PubMed1998

[Evaluation of the correlation between the level of IgG antibodies against mycobacterial A60-antigen and tuberculin reactivity in persons without a history of tuberculosis and in active pulmonary tuberculosis patients].

A measurement of antimycobacterial antibodies levels provides important informations about infection with tubercle bacilli and the development of tuberculosis. A tuberculin test as a manifestation of cellular immunity, has lost in diagnostic value. The aim of the present study was to assess the correlation between the level of IgG antibodies against A60 antigen of Mycobacterium bovis BCG and tuberculin reactivity. 213 persons were involved into the study: 104 subjects without history of tuberculosis in the past (Group I) and 109 active pulmonary tuberculosis patients (Group II)--56 culture positive patients and 53 culture negative patients. In all subjects the A60-ELISA test with the use of Immune kit was performed. All subjects were also tuberculin tested by the Mantoux technique using 2 units of tuberculin Rt23. We found that the levels of anti-60 antibodies in patients with active pulmonary tuberculosis were significantly higher than in controls (median value 43 U/ml and 237.5 U/ml respectively; p = 0.0001). We observed 47.1% and 74.3% tuberculin positive subjects in Group I and Group II respectively. We did not find relationship between the level of IgG anti-A60 antibodies and tuberculin reactivity in tuberculin-positive and tuberculin-negative subjects both in controls and active pulmonary tuberculosis patients. We conclude: 1. There is no correlation between A60-ELISA results and tuberculin reaction status. 2. The serological test can better than tuberculin test differentiate subjects with non-active tuberculous infection from active disease.

Adult↗
PubMed1998

[Use of Polymerase chain reaction (PCR) for detection of Mycobacterium tuberculosis complex from patient materials].

The polymerase chain reaction (PCR) and direct culture were applied for detection of Mycobacterium tuberculosis complex in samples obtained from patients with suspicion of pulmonary and extra-pulmonary tuberculosis. In the reaction of amplification IS6110 was applied as a target region, and PCR reaction products were of the size of 123 bp and 317 bp. A total of 278 samples (158 sputum, 36 urine, 25 pleural effusion, 23 bronchial washings, 8 blood, 4 stomach washings, 3 cerebrospinal fluid, 2 fragments of skin, 2 pleural effusion, and 17 others samples) from 181 patients were tested. Mycobacterium tuberculosis complex was detected by PCR in 144 out of 278 samples and by culture in 68 of 278 samples. The PCR test enabled a rapid and sensitive diagnosis particularly in a number of samples which were negative on culture.

Blood↗
PubMed1998

[Prevalence of COPD in Gdansk sea port workers].

UNLABELLED: The purpose of the study was to evaluate prevalence of COPD in sea-port workers who has been employed with handling phosphoric compounds, sulphur compounds and grain. Smoking habits has been also analysed. Study group consists of 238 persons working in Gdańsk sea-port. Protocol of the study has included: medical history, detailed description of work place, smoking habit analysis and spirometric study. As a spirometric parameters FEV1, FVC, FEV1%VC, PEF, MEF25%VC, MEF50%VC and MEF75%VC has been assessed. In study group 106 persons worked with phosphoric compounds handling, 42 persons with sulphuric compounds handling and 90 persons with grain handling. COPD had been diagnosed in 55 persons--43 smokers and 12 non-smokers. Mean values of FEV1% in groups with equal 10-20 years time-work has been compared. Typical COPD features has been present in 37 persons--25 smokers and 12 non-smokers. COPD has been defined based on medical history, physical examination and no response o FEV1 after bronchodilatator application (Berodual). CONCLUSION: Smoking habits in Gdańsk sea-port workers has been more important factor in COPD development than work place toxicities.

Adult↗
PubMed1997

[Bacterial diagnosis of tuberculosis using the PCR technique].

At the beginning of 80-ties new system BACTEC for mycobacteria culture has been introduced. So time of this culture has been shortened to 1-3 weeks. At the end 80-ties Polymerase Chain Reaction (PCR) technique was started and at present it is used in many laboratories in order to get information about genetic material of mycobacteria in different biological samples. Most often insertive factor IS 6110, located in chromosome Myc. tub. complex, is used. DNA amplification occurs in three stages: 1) DNA denaturation. 2) addition of starters (primers): 3) elongation of starter with d NTP synthesis. PCR technique identifies genetic material (DNA) of mycobacterium which is presented in a given biological sample. Positive PCR result not necessarily means that active disease takes place.

DNA, Bacterial↗
PubMed1997

[Non-sporeforming anaerobic bacteria in bronchoalveolar lavage fluid of patients with pneumonia during the course of lung cancer].

The aim of this study was to assess the occurrence of non-sporeforming anaerobes in bronchoalveolar lavage in patients with pneumonia coexisting with lung cancer. Material consisted of 40 patients with lung cancer. Bronchoalveolar lavage was performed before administration of antimicrobial treatment and obtained material was cultivated in anaerobic and aerobic conditions. Quantitative assessment of anaerobic bacteria and their susceptibility to common antimicrobial agents were also performed. Peptostreptococcus, Prevotella, Fusobacterium and Bacteroides were the most common anaerobes. Among aerobic bacteria, the most frequent were G-negative bacilli. G-negative anaerobes were susceptible to most tested agents, whereas G-positive rods were resistant to metronidazole and tinidazole. This study demonstrates presence of non-sporeforming anaerobic bacteria in lower respiratory tract infections accompanying lung cancer.

Adult↗
PubMed1996

[Occurrence of non-spore forming anaerobic bacteria in the upper airways of patients with chronic obstructive pulmonary disease].

The aim of this study was to assess the occurrence of anaerobic non-sporeforming bacteria upper airways flora in the exacerbation of COPD. Sputum from 35 COPD patients was sent to the laboratory in sterile, filled with CO2 containers and cultured under anaerobic conditions. In all patients bacteriological tests were positive. The most common anaerobic bacteria were as follows: Peptostreptococcus - in 28 pts, Fusobacterium - in 27 pts, Bacteroides - in 26 pts and Prevotella in 24. Less common were: Propionibacterium, Actinomyces, Eubacterium, Porphyromonas and Peptococcus. The susceptibility to most common antimicrobial agents was evaluated. All anaerobic bacteria show a significant sensitivity to amoxicillin either with clavulanic acid or ampicillin with sulbactam. Gram-positive rods were resistant to metronidazole and tinidazole.

Adult↗
PubMed1994

[Thoracoscopy].

The purpose of the work is presentation of own experience in the use of thoracoscopy in patients with pathological pleural changes. In the years 1989-1991 in the Chair and Department of Pulmonology and Tuberculosis, Medical Academy in Gdańsk, 20 thoracoscopies were performed with simultaneous taking of material for histopathological examination. In 15 patients from this group the diagnosis was made confirming the aetiology of pleural pathological changes. It should be stressed that in the group of patients with suspected malignancy, the diagnosis was confirmed in 100% of cases. Thoracoscopy is a useful diagnostic procedure during which aimed pleural biopsy may be performed.

Adolescent↗
PubMed1993

[Characteristics of patients with pulmonary tuberculosis].

The purpose of the study was presentation of the characteristics of patients treated for various forms of pulmonary tuberculosis at the Chair and Department of Pulmonary Diseases and Tuberculosis Medical Academy in Gdansk in the years 1977-1991. On the basis of medical documentation the incidence and age distribution were analysed. The proportions of patients with different forms of tuberculosis and the duration of their treatment were calculated. In the selected years 1977, 1982, 1987, 1991 the results of bacteriological investigations confirming the diagnosis were compared. In the light of the obtained data it was found that in the last 15 years there was an increase in the number of patients aged over 50 years. In the years 1990 and 1991 a higher per cent of cases of miliary tuberculosis and caseous pneumonia was found as compared with the years 1977-1978. Caseous pneumonia was diagnosed in 8.4% of all patients treated for tuberculosis in 1977, while in 1991 this proportion was 14.5%. Positive results of bacteriological examination in 1977 were obtained in 57% of cases, in 1987 in 58.8%, and in 1991 in 35 cases, that is 63.6% of all cases of pulmonary tuberculosis.

Adolescent↗
PubMed1992

[Evaluation of the effectiveness of medical prophylactic procedures applied to workers in ports].

The purpose of the study is an evaluation of the effectiveness of the introduced medical prophylactic procedures among workers employed in zone of pollution with inorganic dusts. Medical and laryngological examinations were done several times in 125 workers of the Maritime Merchant Haven in Gdańsk overloading phosphates, apatites, and crystalline sulphur compounds. In all studied patients systematic procedures were applied of rinsing the nasal and oral cavities with normal saline. A protective gel and also mucolytic drugs, among others Mistabron, were applied intranasally. Toxicological examinations were done of the nasal cavity washings with measurement of the quantity of phosphorus in cm3 before and after rinsing. The phosphorus concentration before the hydrotherapy procedure was X = 12.7 Ug/cm3, and X = 0.65 Ug/cm3 after the procedure. After using of prophylactic measures for two years, a reduction was achieved of the number of workers with respiratory tract diseases by 60% in the Sulphur Overloading Division, and by 34.2% in the Raw Phosphate Fertilizers Overloading Division.

Air Pollutants, Occupational↗
PubMed1992

[Evaluation of the activity of angiotensin I converting enzyme (ACE) and of humoral immunity in patients with active pulmonary sarcoidosis].

In a group of 20 patients with active pulmonary sarcoidosis and in 20 healthy subjects, serum levels were determined of angiotensin I converting enzyme, immunoglobulins, and of the third and fourth complement components. An increased level of IgG and IgM was found in patients with sarcoidosis, as compared with the control group. High ACE levels were observed in the second and third phase of the disease. A tendency was also observed for simultaneous increase of IgG and ACE levels in patients with sarcoidosis.

Adult↗
PubMed1990

[Mycoplasma pneumonia].

Two cases of mycoplasma pneumonias are presented. Initially this etiology was not suspected. Prior to hospitalization they were treated with cefamandole and carbenicillin and cephradine with gentamycin without any improvement in the clinical state of the patients. After serological tests finding a high titer of serum antibodies (1: 2560) the therapy was altered in the first patient to minocycline (in a dose of 200 mg per day for 16 days) and in the second to doxycycline (in a dose of 200 mg per day for 10 days). The first patient improved clinically after two days of altered therapy, the second after 10 days. Both patients were discharged fully recovered after 32 and 36 days of hospitalization.

Adult↗