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Biomedical subjects

L Wilson

Publications and source records attributed to L Wilson.

At least 433 records · Page 24Linked to original sources
PubMed1975

Clobetasone butyrate, a new topical corticosteroid: clinical activity and effects on pituitary-adrenal axis function and model of epidermal atrophy.

Clobetasone butyrate is a new corticosteroid, selected for study because of its combination of good activity in the vasoconstriction test and low systemic activity in animals. Formulated as an 0.05% ointment and cream (Molivate) it was clinically effective in patients with eczema, its activity being significantly greater than that of hydrocortisone 1% or fluocortolone 0.2% (Ultradil). Under conditions that predispose to maximal percutaneous absorption clobetasone butyrate ointment had minimal effect on hypothalamic-pituitary-adrenal function. In an animal model of cutaneous atrophy it caused less thinning of the epidermis than steroids other than hydrocortisone. Clobetasone butyrate 0.05% ointment and cream gave every indication of offering clinically effective topical anti-inflammatory activity with a wide margin of safety.

Administration, Topical↗
PubMed1975

B and T cells in a cat with thymic lymphosarcoma.

The mixed antiglobulin reaction and the formation of nonimmune rosettes with guinea-pig red blood cells (RBC) distinguished feline B and T cells, respectively. In a cat with thymic lymphosarcoma, the cells reacting in these tests formed separate, nonoverlapping populations. The malignant cells were large lymphoblasts replacing the normal thymus and infiltrating local lymph nodes, where they localized only in the paracortical, i.e., thymus-dependent areas. Cells from the nodes could therefore be identified as malignant or normal by their size. The mixed antiglobulin reaction showed that the malignant cells did not carry the surface Ig characteristic of B cells, whereas these malignant cells formed nonimmune rosettes with guinea-pig RBC. Among lymph node cells, most surviving normal and small lymphocytes, from outside the thymus-dependent areas, reacted as B cells. The morphologic evidence therefore corroborated the test results, which indicated that the formation of rosettes with guinea-pig RBC seems a reliable means for the demonstration of T cells in the cat.

Animals↗
PubMed1975

Intravascular hemolysis: a complication of midtrimester abortion: a report of two cases.

Intravascular hemolysis occurred twice in a series of 1582 consecutive intraamniotic midtrimester saline abortions. The hemolysis was associated with consumption coagulopathy and acute renal failure. Both patients made a complete recovery after a prolonged hospital stay; one required peritoneal dialysis. The pathogenesis of this serious complication of saline abortion is discussed but no satisfactory explanation has been reached.

Abortion, Induced↗
PubMed1975

A mixed-immunoglobulin rosette technique for detection of antibody to feline oncornavirus-associated cell membrane antigen.

A mixed-immunoglobulin rosette technique has been developed for the detection of antibodies to feline oncornavirus-associated cell membrane antigens. Lymphoblastoid cells infected with feline leukemia virus were incubated with test sera and then fixed in paraformaldehyde. They were then exposed to a rabbit anti-cat immunoglobulin G serum. Antigen-antibody reactions were detected by mixing the cells with sheep erythrocytes sensitized with cat anti-sheep red blood cell serum; the presence of cat antibody on the lymphoid cells was registered by the formation of sheep red blood cell rosettes around them. The method was shown to be at least 10 times more sensitive than indirect immunofluorescence. A high degree of correlation was shown between the mixed-immunoglobulin rosette and indirect immunofluorescence tests, using both feline leukemia virus-infected cat and dog cells as targets. The results indicate that the tests are likely to be measuring similar reactions. Using the indirect immunofluorescence test we found that 75% of cats with lymphoid neoplasia had no demonstrable antibodies. Twenty-four of such indirect immunofluorescence-negative sera were tested by the mixed-immunoglobulin rosette technique; antibody was shown in at least seven of these sera.

Animals↗