Search PubMed⌕ Search

Biomedical subjects

L Williams

Publications and source records attributed to L Williams.

At least 307 records · Page 17Linked to original sources
PubMed1983

Isolation and characterisation of genomic and cDNA clones for an androgen-regulated secretory protein of rat seminal vesicles.

Testosterone controls the synthesis of seminal vesicle protein F in male rats by regulating the cellular concentration of its mRNA (mRNAF). Phage lambda recombinants have been isolated containing the complete F gene. In addition plasmids have been constructed containing cDNAF sequences some of which are probably full-length (approximately 700 bp). Detailed restriction mapping shows that the F gene is 1.7 kbp long and contains approximately 1.0 kbp of intervening sequence arranged in at least two introns (420 bp and 600 bp). Part of cDNAF has been sequenced showing that the terminal 125 bp of the 3' untranslated region of mRNAF has substantial (greater than 70%) sequence homology with the 3' end of the mRNA coding for another androgen-dependent seminal vesicle protein (protein S). The cloned F gene has been detected in liver and seminal vesicle DNA along with an homologous but structurally different gene. The hormonal control of mRNAF was examined with cDNAF. A pronounced (approximately 3000-fold) differential response to testosterone was observed.

Androgen-Binding Protein↗
PubMed1983

Isolation and characterisation of genes for androgen-responsive secretory proteins of rat seminal vesicles.

Under the influence of testosterone, rat seminal vesicles synthesise large amounts of a tissue specific protein, S. Recombinant lambda clones have been isolated containing overlapping sequences covering a 27.5 kilo base region of the rat genome within which the gene for protein S is located. Recombinant plasmids bearing cDNA sequences for protein S were constructed in pBR328. One (pcS2) contains a 690 nucleotide insert and is probably full length. Detailed restriction maps of the S-gene are presented and the structure was confirmed by analysis of R-loops and heteroduplexes. The S-gene covers a 2 kbp region of the genome and consists of a 5' intron (490 bp) separating a leading exon (120 bp) containing the 5' untranslated region from a central exon (310 bp) containing most of the coding sequence and part of the 3' untranslated region. A larger intron (1100 bp) lies within the 3' untranslated region. The cloned gene is representative of the native gene but the S gene may be heterogeneous. Using pcS2, the hormonal control of S-specific mRNA was examined and a pronounced differential response to testosterone was observed.

Animals↗
PubMed1983

Synthesis of [1-14C]1,2-cyclohexanedione bis(4-diethylenoxythiosemicarbazone) and preliminary biodistribution studies of this potential antitumor agent.

A new compound, [1-14C]1,2-cyclohexanedione-bis(4-diethylenoxythiosemicarbazone) was found to have significant antitumor activity (% T/C = 245) when tested against sarcoma-180 ascites tumor in mice and thus may be a potentially useful drug. The compound can be easily labeled with 14C by employing the straightforward synthetic procedures detailed in this article. Results of the synthesis and purification are presented. Preliminary biodistribution studies of the labeled compound in both normal and tumor bearing mice were performed. The compound, when administered i.p., is rapidly absorbed and localized into most tissues. Urinary and biliary excretion are its major routes of elimination. Based on these studies, continued evaluation is recommended.

Animals↗
PubMed1983

Supplementation of nutritional fluid and electrolyte balance by perfusion of an isolated small bowel loop in a patient with Crohn's disease.

Delayed reanastamosis of small bowel in Crohn's disease has been made possible, largely by parenteral nutrition. We describe a patient with multiple resections resulting in loss of all the small and large bowel except the duodenum and 70 cms of proximal jejunum ending in a jejunostomy and a 150 cm long isolated loop of jejuno-ileum. The short proximal bowel proved adequate for energy and protein nutrition, with small frequent meals but gave rise to a persistent negative balance of water, sodium, potassium and magnesium. This was corrected by overnight perfusion of the isolated loop with an electrolyte solution with added glucose polymer to enhance sodium absorption. The critical rate of perfusion and concentration of glucose polymer were determined by a series of studies. The patient and her husband carried out this regimen for a year at home. The isolated loop was subsequently reanastamosed to the proximal jejunum allowing resumption of normal eating without further electrolyte disturbance.

Journal Article↗
PubMed1983

Tibial dysplasia. A study of the anatomy.

Three amputated legs with tibial dysplasia were studied by radiography, arteriography and anatomical dissection. The radiographic appearances were the same as the Type 1b tibial dysplasia described by Jones, Barnes and Lloyd-Roberts (1978) in that the tibiae were absent but the lower femoral epiphyses were normal. However, our anatomical findings differed from those of Jones et al. since no bony or cartilaginous anlage of the proximal tibia was found in any of the three legs. The pattern of vascular anomaly was identical in the three legs and similar to the findings of Hootnick et al. (1980) in congenital short fibula. Congenital fusion of the subtalar joint was a constant finding. These results support the hypothesis that the arterial and skeletal systems are vulnerable to a teratogenic insult in the fifth week of embryonic life. The bony and arterial anomalies should be borne in mind by the surgeon attempting reconstructive surgery for this condition.

Angiography↗
PubMed1983

Preventing burnout: an alternative approach.

Burnout is a potentially serious dilemma which any practicing pharmacist may encounter. Various suggestions have been proposed to prevent burnout. An alternative approach is presented which involves the pharmacist in a nontraditional role. Pharmacists participate in a 3-month rotation within the Idaho Drug Information Service and Regional Poison Control Center. Pharmacists involved in this rotation believe it is extremely important for expanding their professional roles and preventing burnout.

Burnout, Professional↗
PubMed1983

A DNA polymorphism adjacent to the human apolipoprotein CII gene.

We have used a cDNA clone for human apolipoprotein CII (apo CII) to detect a common DNA polymorphism with the enzyme TaqI. This polymorphism is probably caused by a single base change approximately 2000 base-pairs from the 3' end of the structural gene. In the normal population (n = 90) the frequency of the less common allele is approximately 0.44. No significant differences were observed in the allele frequency in individuals with type IIa, IIb, III, IV and V lipoprotein patterns. There does not seem to be any population association between the TaqI polymorphism and factors that predispose an individual to hyperlipidaemia.

Adult↗