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Biomedical subjects

L Wei

Publications and source records attributed to L Wei.

At least 181 records · Page 10Linked to original sources
PubMed1997

Effect of jiawei shenqi dihuang tang on the content of urinary protein in patients with diabetic nephropathy.

42 cases of diabetic nephropathy (DN) diagnosed as the type of kidney deficiency and blood stasis were treated with Jiawei Shenqi Dihuang Tang. The therapeutic effect demonstrated that various kinds of symptoms and sings were significantly improved. There was significant difference in the qualitative and quantitative examinations of urinary protein between pre- and post-treatment (P < 0.05).

Adult↗
PubMed1997

[Detection of the level of serum antigen recognized by McAbGB2 in breast cancer].

This study sought to verify the levels of serum antigen recognized by McAbGB2 (abbr. serum McAbGB2 antigen) in patients with breast cancer. We adopted the ELISA method and used McAbGB2 to detect the level of serum McAbGB2 antigen in 50 normal persons and 60 patients with breast cancer. The results showed that the level of serum McAbGB2 antigen of 17 preoperative patients with breast cancer was significantly different from that of the normal persons (P < 0.001); the rate of agreement on positivity for the 17 preoperative patients with breast cancer was 88.2% (15/17); the levels of serum McAbGB2 antigen in 8 patients among the 17 preoperative patients with breast cancer decreased from 57.5 +/- 51.3 u/ml (preoperation) to 20.6 +/- 4.98 u/ml (postoperation; the levels of serum McAbGB2 antigen in the other 43 operated patients with breast cancer almost remained normal during post-operative chemotherapy. These suggest that serum McAbGB2 antigen is a marker for breast cancer and can be used for serological diagnosis, treatment and prognosis inspection.

Antibodies, Monoclonal↗
PubMed1997

[Observation of the serum acidic isoferritin levels in patients with hepatocellular carcinoma].

The levels of serum acidic isoferritin (SAIF) in 48 patients with hepatocellular carcinoma (HCC), 30 patients with hepatitis, 28 patients with liver cirrihosis, and 33 healthy subjects were measured by using enzyme-linked immunosorbent assay (ELISA--double-determinant). The result revealed that the SAIF values of HCC, HP and LC did not show normal distribution. The median values were 440 micrograms/L, 21 micrograms/L, 120 micrograms/L and, in normal subjects, 66 micrograms/L respectively. There were statistically significant differences between HCC and the control groups. The sensitivity of SAIF to HCC was 85.46%, with the cut-off point being 250 micrograms/L. SAIF was not correlated to AFP in HCC cases. The values of SAIF had no relationship with the volume of the tumors and the clinical stages. SAIF may be an available and useful serum marker and be beneficial to the diagnosis of early-staged HCC.

Adolescent↗
PubMed1997

[Validity of partial laryngectomy for glottic carcinoma].

To evaluate the effect of partial laryngectomy for the treatment of glottic carcinoma, 126 patients with glottic carcinoma were reviewed. Between 1974 and 1994, 132 patients with glottic carcinoma were treated. Up to Dec. 1994, 126 cases (95%) had been followed-up and analyzed statistically in this paper. According to TNM classification (UICC 1987), 98 (77.8%) of them were T1.2, 28 (22.2%), T3.4. One patient was T4N1, and the rest 125 were No. All patients were M0 on admission. A total of 126 patients underwent partial laryngectomy. Among these patients, 89 were treated with surgical procedures alone, and 4 received pre-operative 33 post-operative, radiotherapy. Survival curves were plotted by the method of Kaplan and Meier. The results showed that three-year, five year and ten-year survival rates for 126 patients were 94.7%, 89% and 86.1% respectively; Those for group of T1.2 were 97.8%, 95.1% and 93.3%; For T3.4 were 83.2%, 62.7% and 52.3%. All patients preserved their voice and were able to communicate by speech after operation. The function of larynx was satisfactory. It was concluded that partial laryngectomy is preferable choice for the treatment of glottic carcinoma.

Adult↗
PubMed1997

[An experimental study on the effects of lingzhi spore on the immune function and 60Co radioresistance in mice].

Lingzhi spore (2 g.kg-1.d-1, 10 times) was orally administered to mice, and studies were carried out on the carbon clearance, delayed-type skin hypersensitivity (DTH), antibody hemagglutinin value and spleen weight. The results showed that Lingzhi spore could increase the immune function, inhibit the reduction of white blood cells and raise the survival rate of mice after they were radiated by 870 rad 60Co.

Animals↗
PubMed1997

[The advances of research for cardiac contractile proteins structure and function].

Cardiac contractile proteins are the material basis of cardiac contractility. This paper introduces the Structures of two major cardiac contractile proteins: myosin and actin, especially the details of the two proteins' structure relevant to their function. At the same time, it describes the myosin and actin proteins' changes in normal and abnormal hearts so as to enrich our mind with knowledge of cardiac hypertrophy and heart failure at the molecular level.

Actins↗
PubMed1997

[Primary study on the use of ovariectomized rabbits for establishment of osteoporosis model].

Purebred New Zealand female rabbits were used in this study for demonstrating the feasibility of establishing rabbit's primary osteoporosis model. Bilateral ovariectomies were performed. Five to eight months after operation, we observed the BMD, weight, hair colour and living condition of the model. After killing the rabbits, we made biomechanical detection and pathologic histomorphometry using the bones. The weight of bone ash, the content of calcium and phosphorus, and the weight of metra were determined. The results showed that BMD, bone bending stress, weight of bone ash, content of calcium and phosphorus in ash, and weight of metra all decreased (P < 0.05); the number of trabecula decreased and the bone cortex became thinner. All these parameters evidenced the primary founding of rabbit's osteoporosis model. This research provides a basis for studying the pathogenesis of primary osteoporosis and evaluating the efficacy of pharmacotherapy.

Animals↗
PubMed1997

[Variation in hepatitis C virus NS 5b nucleotide sequence and its relationship with interferon treatment].

In order to investigate variation in hepatitis C virus (HCV) nonstructural 5b (NS5b) nucleotide sequence and the relationship with interferon treatment, cDNA containing in this region were obtained by reverse transcription-polymerase chain reaction from the sera of 4 HCV RNA positive asymptomatic chronic carriers and 9 chronic hepatitis C patients. The cDNA were then directly analysed by polymerase-chain-reaction-directed sequencing. Compared with three published HCV type II/1b sequences (HCV-J, HC-C2 and HCV-BDS), the homology of HCV was lower in the patients with chronic hepatitis C (93.52% +/- 2.09% average), especially in nonresponders to interferon (92.70 +/- 1.90% average), than in asymptomatic HCV carriers (95.65 +/- 2.61%). In 5 patients, HCV RNA was eliminated by interferon treatment. In the remaining 4 interferon-treated HCV survived patients there were 9-48 nucleotide substitutions in HCV mutants out of 380 nucleotides, resulting 6-20 amino acids changes out of 126 amino acids. These results suggested that NS5b region variants play an important role in maintaining persistent infection and the evolution of NS5b is associated with the effect of interferon treatment.

Adult↗
PubMed1997

[The changes and relation among platelet function, plasma heparin and anti-coagulation-III: activity in patients with hemorrhagic fever with renal syndrome].

Blood platelet count (BPC), platelet adhesive rate (PAdT), platelet aggregate rate (PAgT), plasma heparin and anti-coagulation-III: activity (AT-III: alpha) were determined in 55 cases with hemorrhagic fever with renal syndrome (HFRS). In these patients, decreased BPC, defect PAdT, PAgT, increased plasma heparin as well as decreased AT-III: a were found, moreover, these changes were much notable in patients with severe type. A positive correlation existed between plasma heparin and BPC, PAdT, PAgT in cases with 80% AT-III: alpha or above (r values were -0.4344, -.7157 and -0.5547 respectly, p<0.01). The results suggested that plasma heparin may be one of factors resulting in decreased BPC and defect platelet function in HFRS patients with 80% AT-III: alpha or above.

Adolescent↗
PubMed1997

Using the radial distributions of physical features to compare amino acid environments and align amino acid sequences.

We have performed a comprehensive analysis of the microenvironments surrounding the twenty amino acids. Our analysis includes comparison of amino acid environments with random control environments as well as with each of the other amino acid environments. We describe the amino acid environments with a set of 21 features summarizing atomic, chemical group, residue, and secondary structural features. The environments are divided into radial shells of 1 A thickness to represent the distance of the features from the amino acid C beta atoms. We make the results of our analysis available graphically over the world wide web. To illustrate the validity and utility of our analysis, we used the amino acid comparative profiles to construct a substitution matrix, the WAC matrix, based on a simple summary of the computed environmental differences. We compared our matrix to BLOSUM62 and PAM250 in BLAST searches with query sequences selected from 39 protein families found in the PROSITE database. Although BLOSUM62 was the most sensitive matrix overall, our matrix was more sensitive for some families, and exhibited overall performance similar to PAM250. Our results suggest that the radial distribution of biochemical and biophysical features is useful for comparing amino acid environments, and that similarity matrices based on the geometric distribution of features around amino acids may produce improved search sensitivity.

Amino Acid Sequence↗
PubMed1997

[Primary structure and variability of partial sequences in nonstructural gene 5 region of hepatitis G virus].

OBJECTIVE: To sequence partial genome of hepatitis G virus (HGV) in the sera of patients with different chronic liver disease and paid donors. METHODS: Total nucleic acids were extracted from the sera of 3 paid donors, 2 patients with liver cirrhosis, and 1 patient with non-A-E chronic hepatitis, then subjected to reverse transcriptase-nested-polymerase chain reaction (PCR) with primers derived from the putative nonstructural gene 5 (NS5). PCR products were directly sequenced after purification. RESULTS: Partial HGV NS5 gene of 6 isolates exhibited 87.2% to 93.9% nucleotide sequence identities with 3 reported HGV isolates (GBV-C, PNF2161, R10291) while the nucleotide homologies among the 6 isolates were 90.1% to 93.8%. And 93.6% to 98.7 homolies were found at the deduced amino acid level when comparing the 6 isolates with 3 reported ones, while 93.61% to 98.40% amino acid identities were found among 6 isolates. In the region sequenced, there were 16 conserved proline residues and 8 conserved cysteine residues. CONCLUSIONS: Six isolates of HGV were obtained from different cases and donors. The partial nucleotide sequences, as well as amino acid sequences, of the putative NS5 region of HGV were relatively conserved. This region of the genome may be suitable for the development of diagnosis reagents.

Adult↗
PubMed1997

[Treatment and prognostic factors of epithelial ovarian cancer].

OBJECTIVE: To study the treatment results and prognostic factors of epithelial ovarian carcinoma. METHODS: Retrospective study of 155 cases of ovarian epithelial carcinoma admitted in our hospital since Jan. 1970 to Dec. 1992. All of these cases had postoperative pathological diagnosis and staging according to FIGO. 42 cases had second operations and 4 cases had the third. 149 cases had postoperative chemotherapies except for 6. Chemotherapies were also given to 32 cases after the second operation, while 9 for recurrence. RESULTS: The 2-year, 5-year, 10-year survival rates of stage I were 92.4%, 87.0%, 70.6%; and II 91.9%, 63.6% 47.8%; III 59.9%, 38.2%, 19.2% and IV 25.0%, 25.0%, 0.0% respectively. All 6 cases without chemotherapy after surgery died within 2 years. The results indicated that the prognosis of ovarian epithelial carcinoma was related to clinical stage, cellular grade and tumor residues. The 5-year survival rates for stage I was 87.0% and III 38.2% (P < 0.001); and cell G1 95.0% and G3 11.8% (P < 0.001), while no residual tumor 97.6% and with residual tumor > 2cm 21.2% (P < 0.001) respectively. CONCLUSION: Residual tumor of ovarian epithelial carcinoma < 2cm after primary surgery and chemotherapy right after surgery can improve survival rates.

Adolescent↗
PubMed1997

Primary structure and variability of partial nonstructural gene 5 region of hepatitis G virus.

OBJECTIVE: To sequence partial nonstructural 5 (NS5) gene of hepatitis G virus (HGV) in the sera of Chinese patients with chronic non-A-E hepatitis or hepatitis B virus (HBV) infection. METHODS: Total nucleic acid was extracted from the sera of 35 patients with chronic non-A-E hepatitis and 20 patients with HBV infection, then it was subjected to reverse transcriptase-nested-polymerase chain reaction (RT-nested-PCR) with primers derived from the putative nonstructural gene 5 (NS5) region of HGV. The positive PCR products (994 bp in length) were directly sequenced using the Sang's method after purification. RESULTS: HGV RNA was detected in 1 of the 35 patients (2.9%) with chronic non-A-E hepatitis and 5 of the 20 patients (25%) with HBV infection. All the 6 positive samples were sequenced. These 6 Chinese isolates exhibited 87.21% to 93.92% nucleotide sequence identities with 3 reported isolates (GBV-C, PNF2161, R10292) over the 994 nucleotides examined, while the nucleotide homologies among the 6 isolates were 90.09% to 93.82%. And 93.61% to 98.72% homologies were found at the deduced amino acid level in comparing the 6 isolates with 3 reported ones, while 93.61% to 98.40% amino acid identities were found among the 6 isolates. In the region sequenced, there existed 16 conserved proline residues and 8 conserved cysteine residues. CONCLUSIONS: Six isolates of HGV were obtained from different Chinese patients in this study. The partial nucleotide sequences, as well as amino acid sequences, of the putative NS5 region of HGV in Chinese patients are relatively conserved. The overall degree of nucleotide conservation (90.09% to 93.82%) among Chinese isolates may make this region of the genome suitable for the development of diagnosis reagents.

Amino Acid Sequence↗
PubMed1997

[Diagnostic value of serum IGA-fibronectin aggregates to IGA nephropathy].

In order to demonstrate the diagnostic value of serum IgA-fibronectin (FN) aggregates in IgA nephropathy, serum samples from 91 patients with IgA nephropathy, 14 patients with Henoch-Schonlein purpura, 117 patients with non-IgA nephritis, 24 random in-patients and 234 normal controls were examined for IgA-FN aggregates by using ELISA. The results showed that the level of IgA-FN aggregates increased markedly in the serum of patients with IgA nephropathy and 61.5% of them reached the positive standard. Only 8.8% of the patients with non-IgA nephritis, none of the in-patients with non-renal diseases and 5.1% of the normal controls were beyond the positive criteria. But the level of IgA-FN aggregates were also elevated in the serum of patients with Henoch-Schonlein purpura and lupus nephritis. These data indicate that serum IgA-FN aggregates may be useful in the diagnosis of IgA nephropathy, but Henoch-Schonlein purpura and lupus nephritis should be ruled out.

Adolescent↗
PubMed1996

Delayed xenograft rejection in the concordant hamster heart into Lewis rat model.

The inability to provide an adequate supply of human organs for clinical transplantation has created a strong interest in the use of nonhuman, especially nonprimate, organs. The first biological obstacle confronting such discordant transplantations is a series of violent reactions that result in hyperacute rejection of the xenograft. Significant advances in controlling hyperacute rejection have been achieved recently through the generation of transgenic pig donors bearing human complement regulatory proteins. However, when hyperacute rejection is averted, the xenografts are rejected in 2-70 days in spite of high-dose immunosuppression, by a process collectively termed delayed xenograft rejection. Delayed xenograft rejection is characterized by a refractoriness to conventional immunosuppression, extensive xenoreactive antibody deposition, and cellular infiltration that is dominated by macrophages. We have examined the features of extended host and graft response in the concordant hamster-to-rat xenotransplant model, where such features have historically been obscured by early graft destruction. Hamster hearts transplanted into rats do not encounter hyperacute rejection but are rejected within 3-4 days when xenoreactive antibody titers rise exponentially to levels that elicit a classical antibody- and complement-mediated acute xenograft rejection. We have successfully blocked acute xenograft rejection by a combination of immunosuppressive agents, leflunomide, and cyclosporine. Stopping the immunosuppression resulted in graft rejection that is histologically characterized by extensive xenoreactive antibody deposition and cellular infiltration that is predominantly composed of macrophages. We have noted the similarities between the histopathology of rejection of long-surviving concordant xenografts and that described for discordant xenografts and refer to the process of rejection of concordant grafts that have escaped acute xenograft rejection, delayed xenograft rejection.

Acute Disease↗
PubMed1996

Cerebrovascular amyloidosis in squirrel monkeys and rhesus monkeys: apolipoprotein E genotype.

Some neuropathological changes characteristic of aging and Alzheimer's disease (AD) in humans are present also in senescent non-human primates. The human apoE4 allele is associated with an increased risk of developing late-onset familial and sporadic AD. We found that rhesus monkeys and three subspecies of squirrel monkeys are homozygous for apoE phenotype with arginine at positions 112 and 158 as in human apoE4. However, in both species threonine replaces arginine at position 61 of human apoE. It was previously shown that arginine 61 was critical in determining apoE4 lipoprotein distribution in humans.

Alzheimer Disease↗
PubMed1996

CHEB, a convulsant barbiturate, evokes calcium-dependent spontaneous glutamate release from rat cerebrocortical synaptosomes.

CHEB [5-(2-cyclohexylidene-ethyl)-5-ethyl barbituric acid] is a potent convulsant barbiturate that causes direct neuronal excitation by an unknown mechanism. We have analyzed the effects of CHEB on the release of endogenous glutamate from rat cerebrocortical synaptosomes using an on-line enzyme-coupled fluorimetric assay. CHEB evoked spontaneous Ca(2+)-dependent glutamate release with an EC50 = 14.2 microM and an Emax = 3.2 mumol/min/mg. The non-convulsant barbiturates pentobarbital and phenobarbital evoked significantly less glutamate release at high concentrations. CHEB (30 microM) increased intrasynaptosomal [Ca2+] by 58 +/- 4 nM (p < 0.01; n = 4) above baseline compared to an increase of 5 +/- 4 nM (NS; n = 4) produced by pentobarbital (30 microM). CHEB-evoked glutamate release was inhibited by pentobarbital, phenobarbital, EGTA, CoCl2/CdCl2 and flunarizine, but not by local anesthetics, tetrodotoxin, nitrendipine or omega-conotoxin GVIA. These results demonstrate that CHEB acts as a potent and effective secretogogue for glutamate by a pre-synaptic mechanism that does not require activation of Na+ channels or of L-type or N-type Ca2+ channels. Stimulation of spontaneous glutamate release may contribute to the convulsant properties of CHEB.

Animals↗