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Biomedical subjects

L Wang

Publications and source records attributed to L Wang.

At least 613 records · Page 34Linked to original sources
PubMed2001

[The impact of lateral rhinotomy on nasal airway].

OBJECTIVE: In order to ascertain the lateral operation and medial maxillectomy affect on nasal airway. METHOD: Report 16 cases of nasal cavity or nasal sinus neoplasm who were therapied by lateral rhinotomy and medial maxillectomy. They were observed by acoustic rhinometer (AR) and CT examination in 1-2.5 years after the surgery. RESULT: The feeling of nasal obstruct were disappeared in 3 months after the surgery. The nasal airway resistance(NAR) of the ill nasal cavity was markedly decreased after the surgery, and the total NAR were decreasing unmarkably. The mucous proliferation was notable only in the first year after surgery, and especially on remains of the inferior turbinate or extra wall of nasal cavity. CONCLUSION: Lateral rhinotomy operation and medial maxillectomy have less impact on nasal airway and the functions.

Adult↗
PubMed2001

[The diagnosis and treatment of isolated sphenoid sinus disease].

OBJECTIVE: To detail the underlying symptoms and signs of patients with isolated sphenoid sinus inflammatory disease and enhance the initial diagnostic accuracy. METHOD: 49 patients with isolated sphenoid sinus inflammatory disease were reviewed, and the outcomes of the endoscopic surgery were compared with the non-endoscopic surgery. RESULT: In patients who underwent endoscopic sphenoidotomy, 31 were virtually free from symptoms and 5 still experienced mild headache and hyposmia but their radiographic finding had become normalized. None of them need re-operation within 6-48 months follow-up. However, in patients with non-endoscopic sinus irrigation, only one was free from the symptoms and 5 patients underwent re-operation because of recurrence. CONCLUSION: Nasal endoscopy and CT/MRI scan could provide a precise diagnosis for isolated sphenoid sinus disease and endoscopic surgery is valuable in its treatment.

Adolescent↗
PubMed2001

[The analysis of long-term curative effect in 201 cases of endoscopic sinus surgery].

OBJECTIVE: To analyze the long-term curative effect of endoscopic sinus surgery (ESS), and to study the factors related to the defeat of endoscopic sinus surgery. METHOD: 201 cases (353 sides) of chronic sinusitis and nasal polyps were treated with ESS, and were treated by taking the nasal cavity as a whole unit. RESULT: Following up survey from 12 months to 24 months postoperatively, it was found that 108 cases were cured (53.7%), 82 cases achieved symptomatic relief(40.8%), 11 cases remained failure (5.5%). CONCLUSION: It is very important to improve the ESS technique in order to reduce the recurrence of nasal polyps and sinusitis and to improve the curative effect of ESS, in addition, the treatment after operation is also indispensable to successful surgery.

Adolescent↗
PubMed2001

[Effect of arytenoid adduction operation for vocal function of laryngeal palsy patients].

OBJECTIVE: To evaluate the curative efficiency on vocal function of 56 laryngeal palsy patients underwent arytenoid adduction operation. METHOD: Voice analysis and laryngeal aerodynamical measurement were performed for the 56 laryngeal palsy patients to compare maximum phonation time (MPT), mean flow rate (MFR), voice intensity(SPL), and expiratory pressure(EP) before and after operation. Perceptual evaluation was also performed for comparison by using GRBAS system(G). RESULT: The postoperative average MPT increased by 3.1 times in males and 2.7 times in females. The average MFR decreased apparently from 827.3 ml/s in males and 477.1 ml/s in females before operation to 340.3 ml/s and 158 ml/s after operation, which was statistically significant (P < 0.01). The average SPL increased and the average EP decreased after operation. After operation the average G decreased from 2.8 to 1.7 (P < 0.01) and the percentage of improvement over one grade was 71%. CONCLUSION: From these results mentioned above, it can be concluded that the arytenoid adduction operation might be one of the most effective operative methods to improve glottal incompetence caused by laryngeal nerve paralysis.

Adolescent↗
PubMed2001

[Separation of two isomers in the products of condensation reaction with trimethoxybenzaldehyde as raw material and quick determination of the concentration of its intermediates by high performance liquid chromatography].

The content ratio of the two isomers in the products of condensation reaction with trimethoxybenzaldehyde as raw material is the key factor to improve the productivity of trimethoprim(TMP). A quantitative method using Nova-Pak C18 column (3.9 mm i.d. x 150 mm, 4 microns) and tetrahydrofuran(THF)-water (30:70, V/V) as mobile phase with flow rate of 1.0 mL/min and UV 320 nm detector was established. A good linearity was obtained in the concentration range of 0.01 mg.L-1-10 mg.L-1. The detectable limits of trimethoxybenzaldehyde and alpha-(3,4,5-trimethoxy-benzal)-beta-methoxy-propionitrile were 2.0 micrograms/L and 1.0 microgram/L respectively. The method has been used to analyze real samples with satisfactory results.

Benzaldehydes↗
PubMed2001

[Determination of cholesterol and alpha-tocopherol in eggs by capillary gas chromatography].

A simple and rapid method has been developed for simultaneous determination of cholesterol and alpha-tocopherol in eggs. The method involves alcoholic KOH saponification of the samples, extraction with petroleum ether, and determination by wide-bore capillary gas chromatography. The recoveries of cholesterol and alpha-VE in eggs were 91.0%-93.3% and 82.5%-95.0%, with RSDs of 2.7%-3.1% and 4.9%-6.9% respectively.

Cholesterol↗
PubMed2001

[Determination of polyphenols in tobacco by solid phase extraction and high performance liquid chromatography].

A high performance liquid chromatographic (HPLC) method for the determination of polyphenols in tobacco was studied. Polyphenols can be extracted from tobacco sample by refluxing with 80% (volume fraction) methanol, then subjected to degrease by solid phase extraction with Sep-Pak-C18 column. Chlorogenic acid, rutin, scopoletin, caffeic acid, scopolin and other polyphenols can be separated on a Nova-Pak-C18 column (3.9 mm i.d. x 150 mm) gradiently eluted with methanol and 0.05 mol.L-1 potassium dihydrogen phosphate buffer solution as mobile phase at a flow-rate of 0.5 mL.min-1. Polyphenols were determined at the maximum wavelength of each polyphenol, for chlorogenic acid 326.1 nm, rutin 354.8 nm, scopoletin 344.0 nm, caffeic acid 323.7 nm and scopolin 365.2 nm by photodiode array detector. The main polyphenols in tobacco can be identified with UV spectroscopy. The recoveries of tobacco polyphenols were 94%-105%, and the relative standard deviations were 1.28%-1.49%. This method can be applied to the determination of polyphenols in tobacco with satisfactory results.

Chromatography, High Pressure Liquid↗
PubMed2001

[Transcriptional activator PlcR regulate the expression of multiple genes in Bacillus cereus].

Food-poisoning and some diseases of human can be caused by potential pathogenic factors including phospholipases of c-type, hemolysins, enterotoxins, emetic toxin and others from Bacillus cereus which is an opportunistic pathegon. Generally, the expression of pathogenic genes is completed by a co-regulation in many bacteria. The isolation of a mutant, displaying significant reducing expression of lecithinase and general proteolytic activities, from a transposon-induced bank of B. cereus type strain ATCC14579T were described. Sequencing data revealed that a gene with high homology to transcriptional activator (plcR) of B. thuringiensis was inactivated by insertion in this mutant. The results suggest that plcR regulates the expression of multiple genes in B. cereus.

Amino Acid Sequence↗
PubMed2001

[Prospects on the application of toxicogenomics in assessing the safety of chemical compounds].

Toxicogenomics has been developing with the rapid advances in Human Genome Project in recent two years. The categories and specialty of tox-gene; the application of tox-gene microarray in safety assessment, analysis of gene mutation and gene polymorphism, extrapolation of animal tests results, clarification of mechanism and safety assessment of mixtures; some problems of toxicogenomics need to be solved in spite of its great prospective application.

Safety↗
PubMed2001

[Irradiation of 235 uranium on the growth, behavior and some biochemical changes of brain in neonatal rats].

The effects of enriched uranium on growth, behavior and biochemical changes of brain in neonatal Wistar rats was studied. Neonatal rats were irradiated with a single injection of 2 microliters enriched uranium (containing 0, 1, 5 and 10 micrograms of 235U) into left lateral ventricle of brain at the first day (< 24 h) after birth. The growth and neurobehavior development of the rats was delay and abnormal. The microautoradiographic tracing technique showed that the radionuclide was mainly accumulated in the nucleus, also appeared in the cytoplasm of cells and between cells. The activity of superoxide dismutase (SOD) and the level of endothelin (ET) of cerebellum, cerebral cortex, hippocampus and diencephalons of brain after exposure to alpha irradiation were examined with radioimmunoassay. The data indicated that SOD and ET was elevated by low dose irradiation and distinctly inhibited by high dose irradiation. It was concluded that alpha irradiation could have damages on the developing brain of neonatal rats characterized by changing the sensibility, fragility and compensation of nerve cells.

Animals↗
PubMed2001

[Autogenous bone marrow graft for the management of nonunion of tibia].

OBJECTIVE: To investigate the effect of percutaneous bone marrow graft for the management of nonunion of tibia. METHODS: From March 1996 to June 2000, 56 cases with nonunion of tibia were treated by autogenous bone marrow graft. Among them, there were 38 males and 18 females, aged from 19 to 72 years. A marrow needle was inserted into the site of the nonunion under the X-ray, the autogenous bone marrow was injected into the site of nonunion. Compression bandage and appropriate immobilization material were applied after operation. This procedure was repeated every month, 2 or 3 times was needed. RESULTS: 56 patients were followed-up for 4 months to 4 years and 2 months, averaged 2.8 years. Fracture healed in 53 cases and X-ray displayed fracture line disappeared and a great deal of continuous callus formed, and nonunion in 3 cases. CONCLUSION: Percutaneous autogenous bone marrow graft can play a role in osteogenesis at the site of nonunion. It is easy to aspirate bone marrow and the operation is simple. It has clinical application value for the satisfactory effect.

Adult↗
PubMed2001

[The clinical research of phaco techniqiue and IOL implantation in children cataract surgery].

OBJECTIVE: To investigate the clinical application of phaco technique with amblyopia treatment in children cataract. METHODS: The cataract surgery and intraocular lens (IOL) implantation were done in 21 cases(36 eyes) of children who were from three to 12 years old with amblyopia treatment postoperation. RESULTS: The postoperative corrected visual acuity was more than 0.5 on 12 eyes (33%), from 0.1 to 0.4 on 13 eyes (36%), less than 0.1 on Three eyes (8.3%), incorporation on eight eyes (22%). Strabismus was found on one eye with nystagmus on one eye and central corneal perforation on two eyes. In the follow-up, there were two eyes with platycoria and three eyes posterior capsular opacity with Erbium: Yttium Alminum-Garnet Laser(YAG) laser capsulectomy, especially on the patients with traumatic cataract. CONCLUSION: The chilren cataract surgery with the application of phaco technique and amblipia treatment postoperation were more excellent than those with the traditonal surgical methods. We should attach importance to amblipia treatment after the operation.

Amblyopia↗
PubMed2001

[Vitrectomy and photocoagulation for treatment of proliferative diabetic retinopathy].

PURPOSE: To evaluate the efficacy of vitreous surgery and photocoagulation for treatment of a series of patients with proliferative diabetic retinopathy(PDR). METHODS: A consecutive series of PDR treated with vitrectomy and photocoagulation during February 1997 to May 2000 was retrospectively analyzed. RESULTS: The series included 79 cases (38 male and 41 female) and 104 eyes [oculus dexter(OD), 26; oculus sinister(OS), 28; oculus uterque(OU), 25] with diabetic history for 0.5-37 (8.5 +/- 6.5) years and eye symptoms for 15 days to 1.5 years (1.5 +/- 1.5 years). Retinal photocoagulation was performed in 30 eyes with only once in 26 eyes before vitrectomy. Surgical techniques included standard vitrectomy, neovascular membrane peeling and fragmentation, endo--diathermy and endolaser. Silicone oil was injected in 59 eyes with active bleeding, retinal detachment, or severe ischemia. Cataract extraction was done in 63 eyes, of which 27 eyes with intraocular lens implantation. Visual acuity was light perception(LP), hand movement(HM), count fingers(CF), and > or = 0.02 in 15, 38, 22, and 29 eyes, respectively before operation. Visual improvement achieved in 84 eyes (80.8%) with > or = 0.1 in 41 eyes (39.4%). Visual acuity remained unchanged or decreased in 20 eyes (20.2%). Neovascular glaucoma occurred in two eyes after surgery. CONCLUSIONS: Although the series of patients with PDR without sufficient retinal photocoagulation before operation, vitrectomy and endolaser improved the visual outcome in the majority of eyes. Silicone oil injected in eyes with severe ischemia, active bleeding or retinal detachment may provide chance for prompt laser coagulation after surgery in order to prevent progression of PDR.

Adult↗
PubMed2001

Clinical observation on preservation of lens anterior capsule in reduction of complications associated with silicone oil.

PURPOSE: To observe the effect of preservation of anterior capsule on the incidence of complications associated with silicone oil. METHODS: 82 patients (82 eyes) accepted trans pars plana vitrectomy (TPPV) combined with lensectomy of whom, 30 eyes with preservation of anterior capsule (PAC) and 52 eyes with no preservation of anterior capsule (NPAC). Analysis of the incidence of complications associated with use of oil. RESULTS: The incidence rate was 50.0% in NPAC group, and 23.3% in PAC group (P < 0.025). There were secondary glaucoma (21.1%), band keratopathy (13.5%) and corneal decompensation (9.6%) in NPAC group, while there was none of them in PAC group. CONCLUSIONS: Preservation of anterior capsule is an effective method to reduce the complications associated with silicone oil.

Adolescent↗
PubMed2001

Effect of hypericin on confocal imaging of Ca2+ signaling in cultured human retinal pigment epithelium.

PURPOSE: To investigate the mechanism of the Ca2+ signaling in cultured human retinal pigment epithelial (RPE) cells with the protein kinase C(PKC) specific inhibitor-hypericin stimulation. METHODS: Cultured human RPE cells were analyzed using the fluorescence Ca2+ dye fluo-3 AM and laser scanning confocal microscope(LSCM) after stimulation with 100 nM phorbol 12-myristate 13-acetate(PMA) and (or) 5 concentrations of hypericin(1, 2, 3, 4 and 5 microM). RESULTS: The normal fluorescence in RPE cells was strong and distributed throughout the cells. The nucleus appeared to be more fluorescent than the cytoplasm. After stimulation with PMA alone or 5 concentrations of hypericin, a rapid decrease in flurescence intensity was observed. There was no obvious difference in decreased curve among 5 concentrations. However, after stimulation with a 24 hr preincubation of PMA and 5 concentrations of hypericin, a further decrease was not observed. CONCLUSION: Fluo-3 AM appears to be a good indicator of the change in Ca2+ occurring in RPE cells and hypericin is a strong inhibitor of Ca2+ influx channel. Hypericin has potential as a therapeutic drug for proliferative vitreoretinopathy(PVR), the inhibitory effect on PVR might be caused by blocking the PKC activity and inhibiting Ca2+ influx pathway.

Adult↗
PubMed2001

[Effect of tiangui recipe on hypothalamic proopiomelanocortin mRNA levels in androgen sterilized rats].

OBJECTIVE: To explore the effect of Tiangui recipe (TGR) in androgen sterilized rats (ASR). METHODS: New-born female SD rats were randomly divided into 3 groups. ASR model was established by means of testosterone injection subcutaneously at the age of 9 days, ASR were administered with TGR for 14 days. Around the age of 106 days (proestrus day), all rats were sacrificed, serum total testosterone (TT) and free testosterone (FT) were measured by radioimmunoassay (RIA), and levels of hypothalamic proopiomelanocortin (POMC) mRNA expression in hypothalamus were determined. RESULTS: None of the ASR ovulated and all rats in control group ovulated. The rate of ovulation in TGR group was 58%, the mean-serum concentration of TT and FT in ASR were significantly higher than those in the control group and TGR group (P < 0.01). The hypothalamic POMC mRNA level in ASR model group was significantly higher than that in the control group (P < 0.01), and became normal after TGR treatment. CONCLUSION: The elevated serum androgen levels in ASR could challenge hypothalamic POMC mRNA overexpression via androgen receptor (AR). TGR might reduce serum androgen and lower the expression of POMC mRNA.

Animals↗
PubMed2001

[Poteintation of vincristine-induced apoptosis by tetrandrine, neferine and dauricine in the human mammary MCF-7 multidrug-resistant cells].

AIM: To investigate the poteintation of vincristine-induecd apoptosis by tetrandrine, neferine and dauricine isolated from Chinese medicinal plants in the human mammary MCF-7 multidrug resistant cells. METHODS: The apoptotic cells were detected by fluorescent staining of a combination of Hoechst 33342 and propidium iodide (PI), flow cytometry and agarose electrophoresis. RESULTS: The apoptotic cells induced by vincristine alone accounted for about 10% of all the cancer cells, while the percentage of apoptotic cells induced by a combination of vincristine with tetrandrine, neferine, or dauricine was found to be significantly higher than that by vincristine alone, and their reversal effects were positively correlated with the drug concentration and the exposure time. In addition, tetrandrine was shown to be the most potent in the reversal efficacy among the three compounds to be tested for apoptosis in vitro. CONCLUSION: Tetrandrine, neferine and dauricine showed obvious potenitiation of vincristine-induced apoptosis in the human mammary MCF-7 multidrug-resistant cells.

Alkaloids↗