Lowering of pH from 7.40 to 6.60 significantly decreased the rate of glycerol formation in fat cells incubated with either 1-norepinephrine or theophylline. When these cells were incubated with both 1-norepinephrine and theophylline, the glycerol formation proceeded at maximal rate and was quite similar at pH 7.40 and 6.60. These results indicate that the inhibitory effect of acidosis on the lipolytic action of 1-norepinephrine is exerted on the process which activates lipase.

Acidosis↗

PubMed1965

Increase of O2 uptake during hemorrhage and after transfusion.

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Animals↗

PubMedDate not supplied

Plasma retention and metabolic fate of hemoglobin modified with an interdimeric covalent cross link.

Hemoglobin (Hb) modified with an interdimeric bicovalent cross link using 2-nor-2-formylpyridoxal 5'-phosphate (NFPLP) as the cross-linking agent has an exceptionally low O2 affinity (P50 = 47 torr), enabling it to deliver more oxygen at tissue pO2 than blood. In addition, the covalent cross link prevents dissociation of the HbXL tetramers. By using 3H-labeled HbXL, the present study investigated intravascular retention time of cross linked Hb (HbXL), organ distribution, and routes by which HbXL is metabolized and eliminated from the body. The rats were injected with an i.v. bolus (125-200 mg Hb/kg body weight) of either 3H-labeled HbXL or noncross-linked pyridoxal 5'-phosphate modified Hb (diPLPHb) as a control. Urine and feces were collected daily for up to 9 days. Organs and tissues were harvested either at 9 hr or 9 days and assayed for 3H-label content by standard liquid scintillation counting. Plasma retention of HbXL at this dose was about three times longer than diPLPHb, and no HbXL as such was recovered in the urine. HbXL did undergo metabolic degradation in the body, with labeled fragments (mol. wt. less than 10,000) being excreted by the kidneys and gastrointestinal tract. Total body clearance of the label by 9 days amounted to approximately 83% of the injected dose.

Adrenal Glands↗

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