Search PubMed⌕ Search

Biomedical subjects

L Tissi

Publications and source records attributed to L Tissi.

26 records · Page 2Linked to original sources
PubMed1980

Protection test and early antibody response to tetanus immunization.

The Authors carried out a research work to study the dose-dependence of antibody response in BD2F, mice injected with tetanus toxoid. Experimental data show that it is possible to obtain an earlier resistance at least 14 days after immunization, increasing ten-fold the usual anatoxin dose administered in humans. The protection test toward 4 MLD i.v. injected of tetanus toxin proved a valuable tool for assessing the first appearance of acquired immunity.

Animals↗
PubMed1979

[Technic for studying antitetanus immunity].

The Authors carried out a research work on problems relating to technical aspects to the tetanus immunity tests. Some interesting conclusions have been reached: - Purified and diluted tetanus toxin is best preserved at - 20 degrees C, in pH 7.4 physiological salt solution added with 1% peptone. - Vaccination in small laboratory animals must be done injecting adsorbed anatoxin by a microsyringe, in order to avoid dilution of the original compound. - Protection test using low, i.v. toxin doses is more reliable than serological tests in assessing the acquired resistance level in immuno-prophylaxed animals. - Passive haemagglutination test is much more sensitive when done with turkey instead of sheep red blood cells.

Animals↗
PubMed1978

The state of antitetanus protection in the commune of Perugia.

A serological survey was carried out on the population in the Perugia district to assess the protection level against tetanus. The antitoxin titre was evaluated by indirect haemagglutination test in 983 sera from healthy subjects of both sexes and all age-groups. Only 285 males and 144 females, 33.47% of the subjects, had been immunized prior to the investigation. The research made it clear that the serological antibody level does not always correspond to the anamnestic data on previous immunization. Therefore, it appears that prophylactic treatment against tetanus should be based on objective data, that is, serum antibody titre, in all subjects at risk. This information, thanks to the indirect haemagglutination test, is not difficult to obtain.

Adult↗
PubMed1978

Haemagglutination antibody titre levels which give certain protection against tetanus toxin.

The lowest haemagglutination titre which gives safe protection against tetanus toxin, in sera of vaccinated subjects of both sexes and of all age groups has been assessed, by comparative haemagglutination and neutralization tests. It was established that sera with 1/32 haemagglutination antibody titre have a toxin neutralizing capability of greater than or equal to 0.1 I.U./ml. Lower haemagglutination titers do not assure protective capacity against tetanus toxin.

Animals↗
PubMed1977

Anti-Candida albicans precipitating antibodies in the sera of surgery patients.

In healthy subjects and in subjects undergoing surgical intervention for neoplastic and other diseases, the behaviour of precipitating antibodies against soluble Candida albicans natigens was studied. In 13,7% of healthy controls the Ouchterlony test was positive. In subjects suffering from intestinal tract neoplasia, the percentage of positive tests was 26,6% before and 66,6% after the intervention. In other two groups of patients affected by neoplasia, the per cent positive results of the tests after intervention were, respectively, 50% and 63,1%. Among non neoplastic patients undergoing surgical treatment, after intervention 35% too proved positive. The data were confirmed by P.E.T. (partigen elution test): specific anti-Candida IgG, and in some cases IgA, could be demonstrated. The diagnostic value of positive Ouchterlony's test against superficial soluble Candida antigens after surgical intervention has been discussed.

Antigens, Fungal↗