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Biomedical subjects

L Terry

Publications and source records attributed to L Terry.

At least 19 recordsLinked to original sources
PubMed2000

Establishment and characterization of a breast cell strain containing a BRCA1 185delAG mutation.

OBJECTIVE: The aim of this study was to examine whether cells containing the heterozygous form of a BRCA1 185delAG mutation would exhibit abnormal growth or an altered response to DNA damage. METHODS: A primary culture of human mammary epithelial cells (90P) was obtained from the nontumor breast tissue of a 35-year-old patient who had undergone a mastectomy for removal of a breast tumor. These cells were immortalized (90PE6E7) following retroviral infection with HPV-16 viral E6/E7. genes. Both the 90P cell strain and the cell line were characterized for their ability to grow in culture, form colonies in soft agar, and produce tumors in athymic nude mice compared to normal breast epithelial cells containing wild-type BRCA1. 90P cells were also analyzed for cellular response to gamma radiation and H(2)O(2). RESULTS: These cells were confirmed to contain a frameshift mutation, 185delAG, of the BRCA1 gene. Despite being heterozygous for wild-type BRCA1, the 220-kDa full-size BRCA1 protein was abundantly expressed. 90P and 90PE6E7 cells grew at a similar rate and were anchorage dependent. 90PE6E7 also failed to form tumors in athymic nude mice. Finally, 90P cells exhibited a survival response similar to that of normal mammary epithelial cells to radiation damage and exposure to oxidative stress. CONCLUSION: To our knowledge the 90P cells and the 90PE6E7 cells are the first characterized, non-tumor-derived breast epithelial cells that are heterozygous for the BRCA1 germline mutation 185delAG. Our conclusion is that these BRCA1 mutant cells appear to have growth and stress response characteristics similar to those of normal human breast cells, which is consistent with the hypothesis that loss of heterozygosity must occur to impair putative BRCA1 function.

Adult↗
PubMed1999

Moderate and high intensity exercise training in HIV-1 seropositive individuals: a randomized trial.

HIV-infected individuals are frequently active, but guidelines for exercise in this population lack scientific support, since studies on the effects of exercise training on immunologic variables of HIV-1 positive individuals have shown conflicting results. Exercise capacity, immunologic markers (CD4, CD8 and CD4:CD8 ratio), anthropometric measurements, and depression scores were evaluated to compare the effects of two intensities of aerobic exercise on HIV-1 seropositive individuals. Twenty-one healthy subjects (14 men, 7 women), carriers of the HIV-1 virus (CD4>200 cells x mm(-3)), and inactive for at least 6 months, completed a 12 week exercise training program (36 sessions of 1 h, 3 times per week), in a moderate intensity group (60+/-4% of maximal heart rate) or a high intensity group (84+/-4% of maximal heart rate). Exercise capacity estimated by treadmill time was increased significantly in both moderate intensity (680+/-81 s before; 750+/-151 s after) and high intensity (651+/-122 s before; 841+/-158 s after) groups, but the high intensity group presented a significantly larger increment (p<0.01). There were no significant changes in the immunologic variables, anthropometric measurements or depression scores. Thus, HIV-seropositive individuals that participate in moderate and high intensity exercise programs are able to increase their functional capacity without any detectable changes in immunologic variables, anthropometric measurements or depression scores.

Adult↗
PubMed1999

Development of a maximal testing protocol for the NordicTrack cross-country ski simulator.

PURPOSE: The purpose of this study was to develop a valid VO2max testing protocol for the NordicTrack (NT) cross-country ski simulator. METHODS: To be considered a valid protocol, it was felt that the maximal values obtained from the cross-country skiing (XC) test should be similar to those obtained from a maximal graded treadmill (TM) test. During pilot testing, cadence (strides x min(-1), arm resistance, leg resistance, and grade were individually manipulated to study the effect on measured VO2. A protocol for the NT was developed from the findings of the pilot study, and the results were compared with a TM VO2max test utilizing the Bruce protocol. Thirteen male and 15 female volunteers (aged 22-49 yr) served as subjects and completed both tests in random order. The maximal physiological responses from the XC and TM max tests were compared using paired t-tests. RESULTS: There were no significant (P>0.05) differences in maximal VO2 (42.5 vs. 42.6 mL x kg(-1) x min(-1); 3.27 vs. 3.31 L x min(-1)), heart rates (HR; 185 vs. 185 bpm), or ratings of perceived exertion (RPE; 19.0 vs. 19.1) for the XC and TM tests. There were significant (P<0.05) differences for maximal VE (124.5 vs. 118.6 L x min(-1)) and respiratory exchange ratio (RER; 1.15 vs. 1.21) between the XC and TM tests, respectively. CONCLUSIONS: It was concluded that the testing protocol developed for the NT XC simulator is valid and can be expected to elicit similar maximal values when compared with TM testing.

Adult↗
PubMed1999

Metabolic responses associated with deer hunting.

PURPOSE: Deer hunting is a popular recreational activity with a high rate of cardiovascular events. Previous studies have demonstrated large HR responses during deer hunting. This study compared the HR and metabolic costs of maximal treadmill (TM) exercise with those of hiking while deer hunting and while dragging a deer. METHODS: Healthy male volunteers (N = 16) performed a maximal TM exercise test, a 0.8-km hiking test, and a 0.4-km dragging test over lightly rolling terrain. VO2 was measured by portable spirometry and HR by radiotelemetry. RESULTS: HR averaged 74.0 +/- 7.0% and 89.1 +/- 4.5% of peak TM HR during the hike and drag, respectively. The peak HR observed during hiking and dragging was 83.2 +/- 6.0% and 94.9 +/- 4.2% of peak TM HR, respectively. VO2 averaged 62.2 +/- 15.8% and achieved a peak of 77.2 +/- 19.0% of TM VO2 while hiking. This corresponded to 86.8 +/- 17.3% and 108.1 +/- 22.3% of ventilatory threshold (VT), respectively. VO2 averaged 72.3 +/- 21.0% and achieved a peak of 91.2 +/- 21.4% of peak TM VO2 while dragging the deer. This corresponded to 101.5 +/- 27.7% and 128.5 +/- 26.8% of VT, respectively. The VO2/HR relationship showed significant (P < 0.05) difference between the dragging test and the TM test with a disproportionately high HR. The VO2/HR relationship between the hiking and TM tests was comparable. CONCLUSION: In part, the previously described large HR responses and high rate of cardiovascular complications associated with deer hunting may attributable to the elevated metabolic costs of associated activities.

Adult↗
PubMed1997

The physiological responses to walking with and without Power Poles on treadmill exercise.

Power Poles are specially constructed, rubber-tipped ski poles designed for use during walking. Using Power Poles simulates the arm motion of cross-country skiing, thus increasing the muscle mass used during walking. This study investigated the potential increases in exercise intensity and energy cost associated with the use of walking poles. Thirty-two apparently healthy volunteers (16 men and 16 women) between the ages of 19 and 33 years participated. Each completed a treadmill maximal oxygen consumption (VO2max) test and two randomly assigned, submaximal walking trials (one with poles and one without poles) on separate days. Each walking trial was conducted on a level treadmill, for 20 minutes, at an identical self-selected pace. Expired gases, heart rate in beats per minute (bpm), and ratings of perceived exertion (RPE) were recorded each minute. Results between trials were compared using repeated measures analysis of variance and Tukey's post hoc tests. It was found that walking with poles resulted in an average of 23% (4.4 ml.kg-1.min-1) higher VO2, 22% higher caloric expenditure (1.5 kcal.min-1), and 16% (18 bpm) higher heart rate responses compared to walking without poles on a treadmill. RPE values averaged 1.5 units higher with the use of the poles, and the pattern of responses between conditions was similar for men and women. It is concluded that the use of Power Poles can increase the intensity of walking at a given speed and, thus, may provide additional training benefits to walkers.

Adult↗
PubMed1996

Evoked otoacoustic emissions and auditory selective attention.

The auditory system has an extensive peripheral efferent innervation. The question addressed in this paper is whether the olivocochlear bundle (OCB) efferent system innervating the outer hair cells (OHC) of the cochlea plays a role in selective attention. As evoked otoacoustic emissions (EOAE) provide a measure of the active micromechanical properties of OHCs, they can be used to assess the role of the efferent system in attention. Six experiments using tone-pip EOAEs are reported. In each experiment, EOAEs generated by 1 or 2 kHz tone pips when they were attended were compared with EOAEs to the same stimuli when they were unattended. In three experiments (1-4), a non-linear stimulus difference method was used to record a pure cochlear component of EOAEs. In Exps. 1-5, 1 and 2 kHz tone pips were delivered to the same ear and the difficulty of the subjects' task was manipulated in order to produce a more focussed attentional state or contralateral noise was presented to determine whether attention effects are dependent upon having an already activated efferent system. In Exp.6, the 1 and 2 kHz stimuli were delivered to opposite ears. A total of 70 subjects participated in the six experiments. There were no effects of attention on EOAEs in any of the experiments in the direction of previously reported effects. The results of these first six experiments employing simple attention switches between fixed auditory objects do not support active cochlear involvement in selective attention.

Acoustic Stimulation↗
PubMed1994

High frequency of CD8 alpha alpha homodimer-bearing T cells in human fetal intestine.

Immunohistochemistry on frozen sections was used to identify CD8 alpha alpha cells and CD8 alpha beta cells in human intestine. As observed previously, CD8 alpha beta cells predominate (> 95%) in tonsil and post-natal intestine. However in human fetal intestine (16-24 weeks gestation), almost half the CD8+ cells in the lamina propria are CD8 alpha alpha, and many CD8 alpha alpha cells can be identified in the epithelium. In contrast, in the T cell zones of the Peyer's patches, CD8 alpha beta cells are dominant. The CD8 alpha alpha cells are virtually all alpha beta T cell receptor positive. By analogy with the murine system, these CD8 alpha alpha cells in the fetal gut may be directly derived from the marrow, undergoing thymus-independent differentiation in the gut mucosa.

CD3 Complex↗
PubMed1991

Outcome of patients with blunt trauma transferred after diagnostic or treatment procedures or four-hour delay.

STUDY OBJECTIVE: This study was conducted to assess the outcome of patients suffering multiple injuries from blunt trauma who were transferred to a Level I trauma center for definitive care after special diagnostic or treatment procedures (DTPs) or after a simple delay of four hours at a primary receiving hospital. DESIGN: Retrospective review of aeromedical registry data and clinical chart review. MEASUREMENTS: Aeromedical trauma transfers made during a four-year period were studied. The TRISS method was used to determine predicted survivals for DTP patients, patients transferred after a four-hour delay, and patients transferred promptly. RESULTS: The observed survival for 469 aeromedically transferred patients with blunt trauma was the same as that predicted by TRISS analysis (394 vs 392.5). Survival for 86 patients with DTP was also the same as predicted (62 vs 61.6). Outcomes for nonDTP patients and for 92 patients experiencing a delay in transport of four hours or more were also the same as predicted. CONCLUSION: Early versus delayed decision to transfer patients with blunt trauma did not appear to influence overall outcome. These data suggest that minor delays in recognizing the need to transfer patients with blunt injuries to a trauma center for definitive care may be offset by rapid, skilled transfer and highly specialized trauma care and support the hypothesis that regional trauma center care after initial evaluation and resuscitation elsewhere can be effective even when the timing of transfer is not ideal.

Adult↗
PubMed1990

Hope and spiritual well-being: essentials for living with AIDS.

Without a cure on the horizon there is a need to identify ways to sustain hope and spiritual well-being in patients with AIDS. This article examines the impact of AIDS on the emotional and spiritual health of its victims and summarizes current research findings on spiritual well-being in the ill. It also summarizes the authors' study on hope and existential and spiritual well-being in a group of 65 adult male patients who were either serum positive for the human immunodeficient virus or who had been diagnosed with AIDS Related Complex or AIDS.

Acquired Immunodeficiency Syndrome↗
PubMed1988

Loss of CD45R and gain of UCHL1 reactivity is a feature of primed T cells.

A group of mAb recognizing the 200- and/or 220-kDa determinants (CD45R) of the leukocyte common Ag such as 2H4, WR16, MD4.3, and SN130 cross-block each other showing that they recognize a closely related epitope. The antibody UCHL1 reacts with a 180-kDa determinant of the leukocyte common Ag and exhibits a reciprocal T subset distribution pattern to the CD45R group. Peripheral blood T cells were 40% positive for UCHL1 and 58 to 65% positive for the CD45R antibodies; less than 1% of cells stained for both. On activation of CD45R+,UCHL1- T cells by PHA, up to 40% of cells became positive for both CD45R and UCHL1 by day 3. By day 7, CD45R+,UCHL1- cells fell from 90 to less than 21% whereas UCHL1+,CD45R- cells rose from 2 to 93%. Conversely, PHA-stimulated UCHL1+,CD45R- cells remained UCHL1+,CD45R- during the 7 days in culture showing that phenotypic change was unidirectional from CD45R+ to UCHL1+. In primary allogeneic mixed lymphocyte reactions, activated CD45R+ T cells also showed a change to UCHL1+. When these cells were rechallenged by the original alloantigen, the UCHL1+ cells showed 7- to 20-fold greater proliferation than the CD45R+ cells on day 3 after rechallenge. The recovery of virtually all alloantigen induced secondary proliferative response in the UCHL1+,CD45R- T cell population suggests that UCHL1 identifies a primed population of T cells which may include memory cells.

Antibodies, Monoclonal↗
PubMed1988

Limiting dilution analysis of proliferative responses in human lymphocyte populations defined by the monoclonal antibody UCHL1: implications for differential CD45 expression in T cell memory formation.

Using limiting dilution analysis, we investigated proliferative responses of UCHL1+ and UCHL1- T cell populations to compare the precursor cell frequencies following recall and alloantigen stimulation, and the complexity of cellular interactions within UCHL1+ and UCHL1- populations. We find high frequencies of recall antigen responses among UCHL1+, but not UCHL1- T cells. In contrast, both populations contain similar frequencies of alloantigen responsive cells. Our results are consistent with single-hit kinetics in recall as well as alloantigen responses and show no complex cellular interactions within the responding populations. In conclusion, the difference between the recall antigen response of UCHL1+ and UCHL1- cells observed in conventional proliferation assays is most probably due to a high frequency of recall antigen-responsive UCHL1+ cells and not to suppressive phenomena in the UCHL1- population. These data suggest that memory T cells are largely of the UCHL1+ phenotype. The relation of post-thymic T cell maturation and differential CD45 expression is briefly discussed.

Antibodies, Monoclonal↗
PubMed1988

Phenotypic diversity of the CD45 antigen and its relationship to function.

Monoclonal antibodies (mAbs) have identified further heterogeneity within the major CD4 and CD8 T-lymphocyte subsets. Antibodies to different polypeptides of the CD45 complex separate two largely non-overlapping subsets that may represent stages in the maturation of T cells or separate functionally distinct subsets.

Animals↗
PubMed1987

Progress in T cell biology.

We outline recent work in our laboratories on thymus progenitors, lineages within the thymus, interactions between regulatory and effector lymphocytes, splitting the CD4 (T4) T cell subset, and Ir and Is genes. We highlight the possibilities for future research opened up by the demonstration that certain marrow-derived cell lines can repopulate thymic lobes in culture, and also the deep insight into the logical structure of the lymph node provided by our ability to make an exact comparison between two-cell-type and three-cell-type immunoregulatory clusters.

Animals↗
PubMed1985

An evaluation of an inpatient cardiac patient/family education program.

A study to (1) evaluate the impact of a patient education program on the patient's knowledge regarding his/her illness and (2) identify obstacles to compliance behavior after discharge was conducted over a 2-year period. A population of 342 patients with ischemic heart disease was assigned to an inpatient cardiac patient education program consisting of five daily 1-hour classes. The following patient data were gathered: (1) assessment of the individual's knowledge of his/her illness; (2) selected sociodemographic information, (3) measures of general intelligence and problem-solving ability, and (4) motivation to alter risk factors. A measure of compliance with the prescribed treatment plan was obtained by telephone for all patients 4 weeks after discharge. Patients were randomly assigned such that one group received a pretest and a posttest assessment of knowledge while a second group received only the posttest assessment. A difference score t test on knowledge scores was statistically significant. A regression analysis of predictors of overall compliance scores after discharge demonstrated that indicators of motivation were most highly correlated with compliance. It was concluded that additional research is needed to identify: (1) types of information that enhance the patient's awareness of his/her illness and (2) program structures and techniques that enhance patient motivation to comply with a prescribed treatment plan.

Adult↗
PubMed1984

Effect of propafenone in patients with stable ventricular arrhythmias.

Thirty patients with clinically significant ventricular ectopy were treated with propafenone, a new potent antiarrhythmic agent with membrane stabilizing action. Patients had a minimum mean of greater than 30 ventricular premature beats per hour documented by continuous 48-hour ambulatory ECG recording. Twenty-five patients qualified as responders, defined as greater than 85% reduction of ventricular ectopy compared to baseline, and completed a double-blind placebo-crossover phase. Significant reduction in single ventricular ectopy per hour, paired ventricular ectopy per hour, and ventricular tachycardia beats per hour were observed. Almost total abolition of ventricular tachycardia and paired ventricular ectopy was achieved. Side effects were minimal and well tolerated. A significant prolongation of the PR interval occurred. QRS prolongation and prolongation of the corrected QT interval was observed in some patients, with new left bundle branch block developing in two patients. Long-term efficacy and safety studies will be necessary to determine the ultimate role of this new agent in the selection of antiarrhythmic therapy, but these initial results are encouraging.

Adult↗