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Biomedical subjects

L T Lee

Publications and source records attributed to L T Lee.

52 records · Page 3Linked to original sources
PubMed1969

Alpha-L-fucosidase from a soil bacterium.

Intracellular glycosidases were measured in cell-free extracts obtained by ultrasonic disruption of a gram-negative soil coccobacillus (Chase, 1938). From these extracts, alpha-l-fucosidase was purified about 120-fold by salting out with (NH(4))(2)SO(4), ion exchange chromatography, and gel filtration. The approximate molecular weight of the enzyme was 50,000; its pH optimum was 5. The enzyme was inhibited by l-fucose and split this sugar from a purified acid mucopolysaccharide from chicken chorioallantoic fluid. The acid mucopolysaccharide is identical with a component (host antigen) of the hemagglutinin of influenza virus. Its antigenic reactivity is altered by cell-free extracts of the bacterium, in which the responsible enzyme is thought to be an alpha-l-fucosidase.

Bacteria↗
PubMed1966

Pneumococcal neuraminidase.

Lee, L. T. (Columbia University, New York, N.Y.), and C. Howe. Pneumoccal neuraminidase. J. Bacteriol. 91:1418-1426. 1966.-The elaboration of neuraminidase by pneumococci grown under optimal conditions in liquid medium was studied in relation to the bacterial growth cycle. The enzyme was found free in the culture medium in increasing concentration throughout most of the logarithmic phase of growth, at the end of which enzyme concentration had reached a maximum. Only a small fraction of the total neuraminidase was cell-associated at any time. It appears, therefore, that pneumococcal neuraminidase is actively secreted by dividing cells and does not accumulate solely as a result of cellular autolysis. Neuraminidase in cell-free extracts (types I, III, VII, and XIV) was neutralized both by homotypic and by heterotypic antibody, thus demonstrating it to be a group antigen. The enzyme was separable in agar gel electrophoresis from other protein and polysaccharide pneumococcal antigens. Limited immunochemical data suggest that pneumococcal neuraminidase may be of relatively low molecular weight.

Antibodies↗
PubMedDate not supplied

Age-period-cohort analysis of lung cancer mortality in Taiwan, 1966-1990.

The specific aim of this study was to examine the effects of age, calender period of death, and birth cohort in lung cancer mortality in Taiwan over the period 1966-1990. A log-linear model modified from the method of Osmond and Gardner was used. Age is the most important predictor of lung cancer mortality according to the model. The oldest age group of 75-79 years old had a lung cancer mortality 185.3 and 79.2 times those for the youngest age group of 30-34 for males and females, respectively. There was also a significant cohort effect. The birth cohorts at the highest risk of lung cancer death were those born around 1931 for males and 1921 for females. A leveled off or declined trend of lung cancer mortality was observed for more recent cohorts. Parameters of the period factor showed an increasing lung cancer mortality from 1966 to 1990. In addition to the adverse effect of cigarette smoking, dietary and environmental factors should be taken into consideration in interpreting the results.

Adult↗