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Biomedical subjects

L Suter

Publications and source records attributed to L Suter.

At least 55 records · Page 3Linked to original sources
PubMed1985

[Multiple primary malignant melanomas].

We report on a 54-year-old woman who developed 6 melanomas and 9 lentigines malignae in 5 years. Five of the 6 melanomas were malignant lentigo melanomas. In one case it was not possible to differentiate between malignant lentigo melanoma and superficial spreading melanoma. Intensive exposure of the corresponding localizations (face, arms and back) to sunlight over many years was the only risk factor known in this case. In spite of multiple tumors, the prognosis is relatively good for this patient, because the risk of recurrence or metastasis does not multiply but depends on the prognostically most unfavorable tumor (0.65 mm--level III--with regression zone).

Female↗
PubMed1984

HLA-DR antigen expression in primary melanomas of the skin.

Ninety-three primary malignant melanomas of the skin were typed immunohistologically for the expression of HLA-DR on tumor cells. HLA-DR-positive stroma cells were HLA-DR-negative or only locally positive in most cases. In 36 (39%) of the lesions more than 10% of the tumor cells were stained by two monoclonal antibodies against the nonpolymorphic portion of HLA-DR. HLA-DR-positive tumor cells were often accumulated at the advancing front of the melanoma. The occurrence of HLA-DR-positive tumor cells was related to tumor thickness and level of invasion. Substantial numbers of HLA-DR-positive tumor cells were found in half of the tumors thicker than 1.5 mm and in only 18% of flatter lesions. The highest percentage of HLA-DR-positive tumors was found in the group of melanomas invading the reticular dermis (level IV). The majority of tumors (18/24) that had metastasized within an observation period of 0-32 months were HLA-DR-positive. Regarding the mononuclear cell infiltrate, no correlation between the degree of overall infiltrate and the expression of HLA-DR by the tumor cells was found. The infiltrate within the tumor, however, was more often marked in HLA-DR-positive than in HLA-DR-negative melanomas.

Antibodies, Monoclonal↗
PubMed1984

DNA aneuploidy in congenital melanocytic nevi: suggestive evidence for premalignant changes.

Nuclei with abnormal (aneuploid) DNA content were detected by flow cytometric analysis in 4 of 39 congenital melanocytic nevi and in 0 of 62 acquired nevi. Three of the 4 nevi with DNA-aneuploidy were greater than 20 cm in their largest diameter. We suggest that DNA aneuploidy is an indicator of a premalignant condition in congenital nevi in cases where histologic examination does not reveal any evidence for malignant melanoma. Cells with abnormal DNA content (aneuploid cells) are not uniformly distributed in giant congenital nevi: in one lesion we observed an area with a high percentage of cells with DNA aneuploidy, areas with a low percentage of aneuploid cells, and parts that were found to be normal by flow cytometric analysis. Nuclear pleomorphism was found histologically in the area with the high percentage of aneuploid cells while the areas with the low percentage of aneuploid cells were histologically normal. Thus, flow cytometry seems to be an additional sensitive method for the detection of nuclear abnormalities that are not always apparent by conventional histology. Increase in the relative amount of growth phase (S-phase cells), indicating elevated proliferative activity, was detected in 6 of 39 congenital pigmented nevi and in 6 of 62 acquired nevi.

Adult↗
PubMed1984

The expression of tumor-associated antigens in primary and metastatic human malignant melanoma.

Monoclonal antibodies were raised against cultured melanoma cells and selected for their reactivity with melanoma-associated antigens expressed on various melanoma lines. Of 34 monoclonal antibodies, which were all reacting with melanoma lines only 19 antibodies bound to fresh melanoma tissue. Of the 19 antibodies 10 displayed a broad cross-reactivity with normal cells and structures, whereas 9 had a restricted reactivity. Of these antibodies five types were defined, which detected antigens (nevocellular I, nevocellular II, neural, endothelial, basal cell), which were found on certain normal tissues and structures and on certain tumor phenotypes. On the basis of an extensive study on melanoma biopsies of different stages it became evident, that the endothelial, the basal cell and also HLA-DR antigens were significantly more expressed in high risk melanomas. The basal cell antigen was preferentially expressed in locoregional metastases. By immunization with fresh melanoma biopsies different specificities of monoclonal antibodies were obtained as compared to immunization with established cell lines.

Animals↗
PubMed1983

Heterogeneity of primary and metastatic human malignant melanoma as detected with monoclonal antibodies in cryostat sections of biopsies.

Monoclonal antibodies were generated against established melanoma cell lines and characterized by their reactivity with various sublines. The antibodies selected for their reaction with melanoma-associated antigens were tested on cryostat sections of melanoma tissue from various stages and on other tumors. The reactivity with normal tissues was also determined. Of 30 antibodies reacting with melanoma cell lines 11 did not react with melanoma biopsies. Of the remaining 19 antibodies nine displayed broad cross-reactivity with normal cells and structures and other benign or malignant tumor cells. Among the remaining antibodies five types were defined that detected antigens (nevocellular I, nevocellular II, neural, endothelial, basal cell) found on certain normal tissues and structures and on certain tumor phenotypes. Even though there seems to be a tendency for some antigens to be preferentially associated with certain stages of melanoma, it has not yet been possible to establish any clear-cut correlation between the expression of one of the differentiation antigens and a particular stage or malignancy potential of melanoma.

Antibodies, Monoclonal↗
PubMed1983

Monoclonal antibodies against human malignant melanoma.

Monoclonal antibodies were raised against cultured melanoma cells and selected for their reactivity with melanoma-associated antigens phenotypically expressed on various melanoma lines and their sublines. Of 34 monoclonal antibodies tested which were all reacting with melanoma lines only 19 antibodies were found to bind to melanoma tissues. Of the 19 antibodies 10 displayed broad cross-reactivity with other cells and structures whereas 9 had a restricted reactivity. Of these antibodies 5 types could be defined which reacted with nevocellular antigens (I and II) with neural, endothelial and epidermal cells. The antibodies revealed a great heterogeneity of primary and metastatic melanoma. So far no clear association of the antigens with either primary or various forms of metastatic melanoma could be established.

Animals↗
PubMed1981

Monoclonal gammopathies in Sézary syndrome: a report of four new cases and a review of the literature.

Clinical and laboratory findings of four patients with Sézary syndrome and monoclonal gammopathy are presented. In the literature five cases with the association of these symptoms have been reported. Among the authors' series of 13 patients with Sézary syndrome, monoclonal gammopathy was observed far more often than could be expected from its natural incidence. It appears likely that monoclonal gammopathy is related to Sézary syndrome.

Aged↗
PubMed1981

Eosinophilic pustulosis with pemphigus-like antibody.

A 75-year-old male with eosinophilic pustulosis Ofuji complicated by superinfection with Pseudomonas aeruginosa and Proteus mirabilis is described. This is the first case of eosinophilic pustulosis with an antiepidermal antibody. This antibody was directed against the intercellular substance of the lower epidermis and it was detected by direct immunofluorescence in the patient's normal and lesional skin. As revealed by indirect immunofluorescence the patient's serum reacted with intercellular substance of human lower epidermis but not with guinea pig esophagus. Possibly the detected autoantibody is not characteristic for eosinophilic pustulosis Ofuji but rather an accompanying feature of this case similar to the antiepidermal antibodies found in patients with drug reactions and burns.

Aged↗
PubMed1981

[Folliculitis decalvans].

A 58 year-old-male with folliculitis decalvans, a circumscribed chronic purulent folliculitis with subsequent hair loss and follicular atrophia is described. Staphylococcus aureus haemolyticus could be detected in the lesions. Local and systemic treatment with fusidic acid was effective.

Alopecia↗
PubMed1980

Use of an enzyme-linked immunosorbent assay (ELISA) for screening of hybridoma antibodies against cell surface antigens.

A micro-ELISA for screening of antibodies from hybridoma cultures against surface antigens of human melanoma is described. The technique employs alkaline phosphatase-conjugated protein A and target cells attached to poly-L-lysine-coated microtiter plates. The micro-ELISA is equally sensitive as the radioimmunoassay. Mild glutaraldehyde treatment of cells did not lead to an appreciable loss of antigen activity. The fixed cells can be stored at 4 degrees C for at least 6 weeks. It is concluded that the ELISA is superior to the radioimmunoassay in the following aspects: (1) exclusion of radioactive hazards, (2) speed of performance, and (3) lower costs.

Animals↗
PubMed1980

Atypical tuberous myxedema Jadassohn-Dössekker. Report of a case.

A 48-year-old male patient with atypical tuberous myxedema Jadassohn-Dössekker, characterized by nodules on both upper legs with mucin deposits, is described. Atypical tuberous myxedema Jadassohn-Dössekker can be classified together with lichen myxedematosus and scleromyxedema Arndt-Gottron as normothyrotic myxodermia. It can be distinguished from lichen myxedematosus only by the size of the lesions. The pathogenesis is unknown.

Histocytochemistry↗
PubMed1979

Human malignant melanoma antigenic properties of phenol water extracts.

Tumor-associated antigens were demonstrated in phenol water extracts of human malignant melanoma by migration tests with leukocytes from melanoma patients and controls: in 25 out of 60 melanoma patients weak reactivity was observed, usually stimulation of leukocyte migration, while leukocytes from 37 controls were negative in 100 tests. The frequency of positive reactions did not differ significantly in patients with and without metastases. Humoral antibodies against antigens of phenol water extracts were not detectable in melanoma patients. Rabbits did not produce antibodies against tumor typical substances after immunization with phenol water extracts. Tumor associated antigens of phenol water extracts are presumably not targets of antitumor immune reactions that can influence the clinical course.

Animals↗
PubMed1978

[Isolation of soluble tumor-associated antigens of human malignant melanoma].

Experiments were started with an animal model: Tumor antigens could be solubilized from fibrosarcomas of inbred guinea pigs by extraction with physiological saline. Extracts were fractionated by ammoniumsulfate precipitation, gel filtration, ion exchange chromatography, and polyacrylamide electrophoresis. Skin tests and leucocyte migration assays could detect antigenic activity in a number of fractions different with respect to size and charge. A less heterogeneous antigen could be extracted with phenol-water from guinea pig fibrosarcomas. Tumor associated antigens could be demonstrated in phenol-water extracts of human melanoma by leucocyte migration tests. Weak migration reactions were found with leucocytes from melanoma patients, not with leucocytes from controls. The frequency of positive results was similar in patients with and without metastases. No antibodies against antigens of phenol-water extracts could be detected in sera from melanoma patients. No antibodies against tumor associated substances could be demonstrated after immunization of rabbits with phenol-water extracts. Antigens extracted by phenol-water seem to be weak immunogens. Radioactive antigens were extracted from membranes of labelled cell cultures of human melanoma. Antigenic activity was assayed by double immune precipitation followed by sodium-dodecylsulfate-polyacrylamide-electrophoresis. Yields from available cell quantities were too small for further purification and clinical studies.

Animals↗
PubMed1977

[Ascher's syndrome].

A case of Ascher's syndrome with blepharochalasis and double upper lip is described. It is emphasized that the combined appearance of these two symptoms is essential for the definition of this syndrome.

Blepharoptosis↗