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Biomedical subjects

L Stevens

Publications and source records attributed to L Stevens.

At least 217 records · Page 12Linked to original sources
PubMed1969

The binding of spermine to the ribosomes and ribosomal ribonucleic acid from Bacillus stearothermophilus.

1. The total intracellular concentrations of Na(+), K(+), Mg(2+), spermine, spermidine and RNA were measured in Bacillus stearothermophilus. 2. The binding of spermine to ribosomes and to ribosomal RNA from B. stearothermophilus was studied under various conditions by using a gel-filtration technique. 3. The affinity of spermine for ribosomes and for ribosomal RNA decreased with increasing ionic strength of the medium in which they were suspended. 4. The extent of spermine binding did not change appreciably in the temperature range 4-60 degrees . 5. Optimum binding occurred at about pH7.0. 6. The number of binding sites for spermine on either ribosomes or ribosomal RNA was 0.10-0.13/RNA phosphate group. 7. A high proportion of the intracellular spermine is likely to be bound to the ribosomes in vivo; spermine competes with Mg(2+) on equal terms for sites on the ribosomes.

Amines↗
PubMed1968

Studies on the role of polyamines associated with the ribosomes from Bacillus stearothermophilus.

1. Spermine and spermidine were the main polyamines detectable in Bacillus stearothermophilus. 2. When grown at 65 degrees B. stearothermophilus contained lower concentrations of polyamines per mg. of RNA than when grown at 45 degrees or at 55 degrees . 3. Ribosomes isolated from B. stearothermophilus in 0.01m-tris-hydrochloric acid buffer (pH7.4)-0.01m-magnesium chloride contained sufficient polyamines to neutralize between 4% and 9% of their RNA phosphorus. 4. Removal of polyamines from the ribosomes by dialysis against m-potassium chloride did not appreciably alter the hypochromicity or thermal denaturation profiles of the ribosomes when measured in 0.01m-tris-hydrochloric acid buffer (pH7.4)-0.01m-magnesium chloride, though it did cause a loss of ribosome particles sedimenting at greater than 78s. 5. When ribosomes were dialysed against acridine orange solutions acridine orange bound to the ribosomes and did not displace spermine, but when a mixture of ribosomal RNA and spermine was dialysed against acridine orange the acridine orange displaced the spermine. It is concluded that polyamines in the ribosomes are less accessible for displacement by acridine orange than when polyamines are bound to ribosomal RNA.

Acridines↗
PubMed1967

Studies on the interaction of homologues of spermine with deoxyribonucleic acid and with bacterial protoplasts.

Four homologues of the naturally occurring polyamine spermine, of the type H(2)N.[CH(2)](3).NH.[CH(2)] (n).NH.[CH(2)](3).NH(2) where n=2, 3, 5 and 6, have been synthesized. Their ability to stabilize Escherichia coli protoplasts against osmotic lysis was compared with that of spermine. All homologues were approximately as effective as spermine. The effect of low concentrations of the homologues on the T(m) of calf thymus DNA and of Aerobacter aerogenes DNA in 0.03m-sodium chloride-1mm-potassium dimethylglutarate buffer, pH6.2, was tested. The increase in T(m) for a given concentration of amine was found to be n=5>n=4 and n=6> n=3>n=2. When calf thymus DNA in 0.15m-sodium chloride-15mm-sodium citrate was used spermine gave the highest increase in T(m). It is concluded that the stabilization of E. coli protoplasts by tetra-amines is a non-specific effect independent of chain length, whereas the elevation of T(m) of DNA is a more specific effect which depends on chain length.

Amines↗
PubMedDate not supplied

Variation in the production and distribution of substituted benzoquinone compounds among genetic strains of the confused flour beetle, Tribolium confusum.

Insects often produce chemicals, such as defensive compounds, whose quantity and distribution can affect their fitness. For evolution to produce adaptations, chemical production must be genetically variable. Here we report the results of a study using high-performance liquid chromatography to quantify two important chemical secretions of the flour beetle Tribolium confusum, methyl-1, 4-benzoquinone (MBQ) and ethyl-1,4-benzoquinone (EBQ). Our results show a distinct difference in the production of the compounds among four genetically distinct strains of T. confusum (b-+, b-I, b-IV, b-Pakistan) with an unusually high amount measured for the b-Pakistan strain. By measuring internal and external benzoquinone levels separately, we were also able to detect differences in production and distribution of the compounds between the strains. Some strains secrete more of the chemicals, whereas other strains appear to sequester the compounds within their bodies. The sexes also differ in total quinone production as well as in their internal to external benzoquinone ratios, suggesting the trait is sex influenced. Finally, a consistent correlation in the amounts of MBQ to EBQ in individual beetles suggests that the substituted benzoquinones share a common precursor or pathway.

Animals↗
PubMedDate not supplied

The Medicaid market.

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Community Health Centers↗