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L Steele

Publications and source records attributed to L Steele.

86 records · Page 5Linked to original sources
PubMedDate not supplied

Chromatographic investigation of the glycosylation pattern of alpha-1-acid glycoprotein secreted by the HepG2 cell line; a putative model for inflammation?

In certain pathophysiological conditions, such as rheumatoid arthritis, there are alterations in the glycosylation pattern of the acute phase protein, alpha-1-acid glycoprotein (AGP). These changes are likely to be functionally significant, however, verification of the latter role requires a system which reflects in vivo glycosylation changes in AGP and also produces sufficient quantities of the protein for further study. The human hepatoma cell line HepG2 is documented as displaying a shift in the glycosylation pattern of glycoproteins from normal state to acute phase after stimulation with inflammatory mediators. We have isolated AGP from the culture medium of HepG2 cells both before and after stimulation with a cytokine preparation and analysed the glycosylation pattern of each preparation, after enzymatic release, by high pH anion-exchange chromatography. Before stimulation, the glycosylated population was similar to a profile of AGP isolated from normal plasma; however, cytokine stimulation resulted in a shift to a profile which was consistent with that of AGP from a rheumatoid arthritis sufferer. Thus a HepG2 cell culture system is capable of being a crude model of the changes in glycosylation of acute phase proteins although it has a tendency to produce oligosaccharide chains which are not fully sialylated.

Animals↗
PubMedDate not supplied

Fatiguing illness among employees in three large state office buildings, California, 1993: was there an outbreak?

UNLABELLED: The objective was to determine if a cluster of chronic fatigue syndrome (CFS)-like illness had occurred among employees in two large state office buildings in northern California, and to identify risk factors for and features of fatiguing illness in this population. DESIGN: case-control study. POPULATION AND SETTING: Over 3300 current employees in two state office buildings and employees in a comparable "control" building. Information was collected on demographic and occupational variables, the occurrence of fatiguing illness for at least one month in the previous year, and the presence of 36 symptoms. A total of 3312 (82%) of 4035 employees returned questionnaires. Overall, 618 (18.7%) persons reported fatigue lasting at least one month; including 382 (11.5%) with fatigue of at least six months' duration and 75 (2.3%) with symptoms compatible with a CFS-like illness. Independent risk factors for fatigue lasting one month or longer were found to be Native American ethnicity (OR 2.4, CI 1.1,5.3), Hispanic ethnicity (OR 1.7, CI 1.3,2.3), female sex (OR 1.5, CI 1.2,1.9), gross household incomes of less than $50,000 (OR 1.3, CI 1.1,1.6), and less than a college education (OR 1.3, CI 1.1,1.6). Similar risks were observed for persons who reported fatigue lasting six months or longer. Female sex (OR 3.2, CI 1.7, 6.4) was the only independent risk factor found for those persons classified as having a CFS-like illness. Case prevalence rates for all three categories of fatigue, as determined by multivariate analysis, were not significantly different among buildings. Despite finding a substantial number of employees with fatiguing illness in the two state office buildings, the prevalence was not significantly different than that for a comparable control building. Previously unidentified risk factors for fatigue of at least one month and at least six months identified in this population included Hispanic ethnicity, not having completed college, and income below $50,000.

Adult↗
PubMedDate not supplied

Impact of hospital care on incidence of bloodstream infection: the evaluation of processes and indicators in infection control study.

The Evaluation of Processes and Indicators in Infection Control (EPIC) study assesses the relationship between hospital care and rates of central venous catheter-associated primary bacteremia in 54 intensive-care units (ICUs) in the United States and 14 other countries. Using ICU rather than the patient as the primary unit of statistical analysis permits evaluation of factors that vary at the ICU level. The design of EPIC can serve as a template for studies investigating the relationship between process and event rates across health-care institutions.

Bacteremia↗
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The reunion.

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History, 20th Century↗
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Sweeping changes.

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Guidelines as Topic↗
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Who needs nursing care?

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Activities of Daily Living↗
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The scare factor.

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Acquired Immunodeficiency Syndrome↗
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Aiding understanding.

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Acquired Immunodeficiency Syndrome↗
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Mammography and Pap smear screening of Yaqui Indian women.

The Pascua-Yaqui Tribe of Arizona receives its health care services at a local neighborhood health center in Tucson and a satellite clinic located on the reservation. Using a computerized data base from the health center, the authors determined the use rates by Pascua-Yaqui women ages 35-65 of the Papanicolaou smear and mammography screening. Among active users of the health center, 31-36 percent had received a Papanicolaou smear, according to the yearly data bases examined from 1986 to 1990, while 65 percent of the women had received at least one smear test over the entire 5-year period. Regarding mammography screening, 41-43 percent of the women ages 50-65 had received a mammogram in the years studied, and 51-58 percent of the women ages 40-49 had been screened. In all, 67 percent had received at least one mammogram during the 1988-90 period when the center offered mammography. This population of 35-65-year-old American Indian women, for whom financial access is not a barrier, were receiving Papanicolaou smears and mammograms at rates comparable with other segments of the U.S. population but at lower rates than those recommended by the American Cancer Society and National Cancer Institute. The challenge for the health center is to reach those women who are eligible for services but do not use them and to address the nonfinancial barriers to care such as language, transportation, and gender-specific issues.

Adult↗
PubMedDate not supplied

Prevalence of cardiovascular disease risk factors in a southwestern Native American tribe.

A cross-sectional study was conducted among the Pascua Yaqui Indian tribe in Tucson, AZ, in 1990 to document the prevalence of cardiovascular disease risk factors. Cardiovascular disease is the leading cause of mortality for Native Americans and for members of the Pascua Yaqui tribe specifically. A total of 230 randomly selected adults, ages 25-65 years, who were listed as members on the tribal roll, participated, resulting in a 73-percent participation rate for those contacted. The five risk factors studied included diabetes, hypertension, hypercholesterolemia, obesity, and smoking. Only 14 percent of participants had none of the risk factors; 52 percent had two or more factors. Obesity was the most prevalent, being present in 69 percent of the women and 40 percent of the men, followed by diabetes, 35 percent of men and 39 percent of women. Twenty-six percent of the population had hypertension, and 43 percent of men were smokers, compared with 24 percent of women. Hypercholesterolemia was present in 19 percent of men and 14 percent of women. The rates of diabetes, obesity, hypertension, and smoking documented in this tribe are relatively high and can serve as a baseline for evaluating future prevention efforts.

Adult↗
PubMedDate not supplied

Home health care for cancer patients: insights from the American Indian community.

With the confounding effects of an aging population, as well as advances in the treatment of cancers, the cure rates and associated recovery time for patients have increased. In the past, recovery from cancer treatment was often associated with extended hospital stays. However, shorter lengths of stay and the use of home health care is now the accepted standard of care. For the recovering patients, and their families, sensitivity to their cultural needs may often be overlooked given the diverse nature and background of home health care providers. Within the Indian Health Service, the cultural needs of the patients are taken into account. Through this paper we hope to provide a description of this unique program of home health care for American Indians who live on or near reservations and trust lands and Alaskan Natives who live in traditional villages.

Alaska↗
PubMedDate not supplied

A prospective study of insulin sensitivity and glucose metabolism in women using a continuous subdermal levonorgestrel implant system.

OBJECTIVE: Decreased insulin sensitivity and deterioration in oral glucose tolerance curves have been reported with oral contraceptive use, particularly with those containing levonorgestrel. However, the subdermal levonorgestrel systems release a lower dosage of steroid and avoid the first pass through the liver that occurs with oral administration. We hypothesized, therefore, that continuous subdermal levonorgestrel has no significant effect on insulin sensitivity and other indices of glucose metabolism. METHODS: Nine women desiring a subdermal hormonal contraceptive system were recruited for this longitudinal prospective study. Each subject underwent a 3-hour frequently sampled intravenous glucose tolerance test immediately before insertion and after 6 months of continuous use. Insulin sensitivity and glucose effectiveness were determined using a mathematical minimal modeling approach. Glucose clearance and fasting and peak levels of glucose and insulin were examined as well. The matched-pair data were analyzed using paired t tests. RESULTS: There was no significant change in insulin sensitivity, glucose effectiveness, glucose clearance, or fasting or peak insulin levels. Fasting glucose levels remained within a normal range. CONCLUSIONS: Subdermal levonorgestrel appears to have no significant effect on tissue insulin sensitivity or glucose metabolism after 6 months of continuous use.

Adolescent↗