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Biomedical subjects

L Stanciu

Publications and source records attributed to L Stanciu.

69 records · Page 4Linked to original sources
PubMedDate not supplied

Some aspects of cellular and humoral immunity in bronchial asthma and allergic rhinitis.

Blood lymphocyte subpopulations were investigated in 50 patients with allergic diseases (38 with allergic rhinitis and 12 with allergic bronchial asthma). The total T lymphocyte (Tt) count (E rosette test), the active T lymphocyte (Ta) count (active E rosette test) and the B lymphocyte count (EAC rosette test) were determined and the results were correlated with: 1) lymphocyte sensitization in vitro to different allergens and to PPD; 2) circulating immune complexes; 3) serum histaminopexic capacity. In allergic rhinites a decrease of Tt and B lymphocytes and an increase of Ta lymphocytes was observed. In allergic bronchial asthma the Tt lymphocyte were found reduced. Lymphocyte reactivity to PPD in vitro was present in 100% of the cases and in vitro lymphocyte sensitivity to several allergens was observed in most of the cases. The histaminopexic capacity was absent or low in 75% of the patients with allergic rhinitis and in 66.6% of the patients with allergic bronchial asthma. The involvement of cellular mediated immunity in allergic diseases is discussed.

Adult↗
PubMedDate not supplied

Test of leukocyte migration inhibition. 1. Value of the test in the diagnosis of certain immune diseases.

The test of leukocyte migration inhibition (LMI) being a specific and sensitive method for the detection of cell-mediated immunity, its ever increasing use in the clinic necessitates a more accurate estimation of its actual value. The technique used in this study is that of leukocyte migration in capillary tubes according to Soborg and Bendixen. The test was performed in three groups of patients: A--with drug intoxication; B--with chronic post viral hepatitis and C--with alcoholic liver disease. The results obtained showed that in the patients with drug allergy the LMI test using the incriminated drugs as antigens was positive in 83% of the cases, in the patients with chronic post viral hepatitis the cellular migration inhibition to the HBsAg was observed in 35% of the cases and in alcoholic liver disease lymphocyte sensitization to ethanol was present in 28.7% of the cases. It is concluded that in drug allergy the LMI test is useful and has diagnostic value being preferable to other in vitro determinations and even, in certain cases, to the skin tests. In chronic liver diseases the value of the test is limited to investigation purposes as it can reveal the degree of involvement of cell-mediated immunity in the pathogenesis of disease.

Drug Hypersensitivity↗
PubMedDate not supplied

Virological and immunological study of 20 patients with Balkan endemic nephropathy.

A virological and immunological study was carried out in 20 cases of Balkan endemic nephropathy (BEN) belonging to two distinct geographical areas of a Romanian county. Cytopathogenic agents could be isolated in the BHK-21 cell line from 14 urine samples and from one kidney biopsy specimen. Sera from BEN patients gave autologous and heterologous neutralization reactions against several cytopathogenic agents, within the same or from the other geographical area. Humoral and cell-mediated immunity tests against standard antigens and one of the cytopathogenic agents revealed a general preservation of immune reactivity in BEN patients.

Adult↗
PubMedDate not supplied

Circulating immune complexes in chronic hepatitis as related to the presence of HBsAg.

The relationship between the presence of circulating immune complexes (CIC) and of HBsAg in the serum of patients with chronic liver diseases was studied in a group of 78 patients of whom: 16 with chronic persistent hepatitis (CPH), 39 with chronic active hepatitis (CAH) and 23 with liver cirrhosis (LC). The results showed a clear relationship between the incidence of CIC and the presence of HBsAg in the patients with persistent or active chronic hepatitis but not in those with liver cirrhosis. The presence of HBsAg was detected in the composition of CIC in only part of the cases (10/25 patients). This low incidence suggests that in chronic hepatitis other antigens can also participate in the formation of CIC.

Antigen-Antibody Complex↗
PubMedDate not supplied

Test of leukocyte migration inhibition. II. Comparative study of the techniques in capillary tubes and in agarose.

The object of the paper was to estimate the advantages and disadvantages of two techniques of the leukocyte migration inhibition (LMI) test (in capillary tubes and in agarose) for the in vitro testing of delayed type hypersensitization. The capillary technique was carried out according to Soborg and Bendixen and the agarose technique according to Clausen. The antigen used in both techniques was PPD in a concentration of 10 micrograms/ml. The techniques were performed in parallel in two groups of individuals: 15 healthy controls and 20 patients with chronic hepatitis. It was observed that in the control group sensitization to tuberculin in vitro was detected by the capillary technique in 7 of the 15 subjects (47%) and by the agarose technique in 4 of the 15 subjects (27%). In chronic hepatitis sensitization to PPD was detected, by the capillary method, in 11 of the 20 patients (55%) and by the agarose method, in 9 of the 20 patients (45%). The results were consistent by the two methods in 18 of the 20 patients. In conclusion the LMI test in capillary tubes is preferable to that in agarose being simpler, more rapid, more sensitive and less expensive.

Adolescent↗
PubMedDate not supplied

Active rosette-forming lymphocytes in chronic hepatitis.

Lymphocyte subpopulations (total T lymphocytes, active T lymphocytes, late T lymphocytes, B lymphocytes), leukocyte migration inhibition test, serum HBsAg, serum immunoglobulins (IgG, IgA, IgM) were determined in 15 chronic active hepatitis patients and 35 chronic persistent hepatitis patients (diagnosed clinically, biochemically and histologically) and 10 healthy subjects who had acute B viral hepatitis three years ago. A decreased number of total T lymphocytes has been found in HBsAg-seropositive chronic hepatitis. An increase of relative and absolute number of active T lymphocytes was noticed in HBsAg-seronegative healthy persons with a past history of acute viral hepatitis B.

Acute Disease↗
PubMedDate not supplied

Immunologic investigation in cardiomyopathies.

A group of 6 patients with congestive cardiomyopathy were investigated from the immunologic pint of view. Changes of serum immunoglobulins with increase of IgA and IgM were observed in all the patients. The immunofluorescence test in myocardial fragments, taken by transparietal biopsy from the six patients, confirmed the presence of IgA and IgG in two cases. A pathogenic immunologic mechanism is discussed in cardiomyopathies and especially in the alcoholic one.

Adult↗
PubMedDate not supplied

Ergonovine test in coronary disease.

The authors present the role of the Ergonovine test in establishing the diagnosis of the angina produced by coronary spasm. ECG alterations induced by Ergonovine, like transparietal ischemia (monophasic wave) or subendocardic ischemia, not only make possible a correct diagnosis, but they also underline the mechanism of angina and the therapeutical measures. Severe arrhythmias and the danger of sudden death are the two elements illustrating the importance of this coronary spastic mechanism. Nitroglycerine, isosorbidinitrate and calcium antagonists are the drugs of election for the prevention and also for the treatment of coronary spasm.

Adult↗
PubMedDate not supplied

The role of CD8+ T cells in the pathogenesis of atopic asthma.

The role of the CD8+ T cells, a specialized subset of T cells, in the pathogenesis of atopic asthmatics is presented on the basis of the latest data. Thus the authors discuss the regulatory role of the CD8+ T cells in the synthesis of IgE, the environmental factors which activate preferentially the CD8+ cells as well as other factors such as histamine, the leukochemoattractant factor, PGE2, IFN gamma and IL-4 which also contribute to the activation of these cells thus demonstrating their role of down-regulating the immune response in asthmatics without the T helper or T cytotoxic.

Animals↗
PubMedDate not supplied

The prognostic significance of arterial blood pressure in liver cirrhosis.

A decrease of the blood pressure (BP) due to the changes in the regulatory mechanisms of blood pressures homeostasis is frequently observed in cirrhosis. The present work studied the blood pressure profile of the cirrhotic patients and estimated the influence it might have on the survival prognosis at one year. A lower mean blood pressure: 8.25 +/- 1.5 cm Hg is observed versus a control group: 9.8 + 2.0 cm Hg (p < 0.001). The decrease is due to the patients with severe liver impairment (Child class C). The survival is poor in cirrhosis with hypotension (systolic blood pressure < 9 cm Hg): 75 +/- 10%, than in patients with systolic blood pressure between 9 and 11 cm Hg (survival rate 91 +/- 6%) and patients with systolic blood pressure over 11 cm Hg (survival rate 88 +/- 6%) (p < 0.001).

Adult↗