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Biomedical subjects

L Sréter

Publications and source records attributed to L Sréter.

At least 19 recordsLinked to original sources
PubMed2001

Circulating tumor cell clusters in the peripheral blood of colorectal cancer patients.

PURPOSE: Recently several reverse transcription-PCR techniques have been proven to be useful for the detection of circulating micrometastases. However, this way intact cell clusters that were found in animal experiments of prognostic value could not be detected. In this study, evaluation and modification of a commercial, cytokeratin-based, immunomagnetic cell separation method was performed for the detection of intact cell clusters in colorectal carcinoma patients. EXPERIMENTAL DESIGN: Thirty-two colon cancer patients (6 were in Dukes stage B, 13 in stage C, and 13 in stage D) and 20 healthy donor samples were evaluated. Immunomagnetic cell separation was performed from the buffy coat of peripheral blood samples (20 ml) using the Carcinoma Cell Enrichment Kit (Miltenyi Biotec, Bergisch Gladbach, Germany), avoiding any filtering steps. The enriched cell fraction was cytocentrifuged and immunocytochemically labeled using a pancytokeratin antibody (MNF116; Dako). RESULTS: Of 20 healthy samples, 2 contained one cytokeratin-positive cell. Of 32 single samples from malignant cases, 24 showed cytokeratin-positive cells. Tumor cell clusters, mixed-cell doublets (one cytokeratin-positive and -negative cell), and mixed-cell clusters were detected in 22 of 24 patients. In six cases, cytokeratin-positive dendritic-like cells were detected. Follow-up data indicate that chemotherapy cannot destroy all of the circulating tumor cell clusters. CONCLUSIONS: Using the methods presented, we could detect circulating colon cancer cells and cell clusters in colon carcinoma patients. Similar cellular structures were described previously only in rats. Present data prove that such structures are present in human colorectal cancer, too.

Cell Adhesion↗
PubMed2000

Detection of a b3a3 type chimera in chronic myeloid leukemia by double hybridisation of RT-PCR products.

The standard RT-PCR method performed on RNA of a chronic myeloid leukemia patient resulted in a product of unusual size. Hybridisation to a probe containing the a2 sequences yielded a very faint band. Rehybridisation of the same blot to b3 sequences has given a firm signal. Based on the size of the product and on results of hybridisation tests, a translocation, resulting in a b3a3 juxtaposition, was supposed. The direct sequencing of the RT-PCR product has proven this hypothesis. We concluded, that double hybridisation of RT-PCR products of standard methods is a useful tool in detection of rare bcr-abl chimeras.

Female↗
PubMed1999

[The role of nutrition in the pathogenesis and prevention of oncologic diseases].

Nutrition influences the etiopathogenesis of oncological diseases by several mechanisms: the food may contain carcinogens and the organism is able to produce them also in vivo from some foods. The mutagens in the warmed or heated foods, several food-additives and other accessory ingredients of the food chain can influence the carcinogenetic process. From the epidemiological point of view the role of ethanol is very important in the etiology of tumours: its complex cocarciogenic effect is based upon its influence on the DNA metabolism and the hormonal status. The anticarcinogen nutritive factors like vitamin A and retinoids, vitamin D and selen have important anticarcinogan potency based on epidemiologic and experimental result. The anticarcinogenic effect of fibers is complex and multifactorial. The phytochemicals promise a lot in anticarcinogenesis, with their help we may gain a possibility to prevent cancer. The theoretical general dietary principles to reduce cancer risk are summarized. It may be important to change dietary habits also after diagnosis of cancer: it is proved in some tumours that with an ideal nutrition the recidive-free survival is longer. The risks, combined with dietary factors can be effectively and rationally diminished by primary prevention: that is why information and education of the population should have a basic role in primary prevention.

Carcinogens↗
PubMed1999

[Serologic study of human herpesviruses 6 and 7 in lymphoma patients].

DNA sequences, antigens and elevated antibodies to HHV-6, and DNA sequences of HHV-7 in patients with Hodgkin's disease and non-Hodgkin's lymphoma have been detected. It is not known whether HHV-6 variants A and B, and HHV-7 contribute to the malignization by different ways, there is any interaction between these viruses, and their primary or recurrent infections occur during the disease progression. Total and high avidity IgG, IgM to HHV-6A, HHV-6B and HHV-7 were quantitated simultaneously in the sera of 12 patients with lymphomas and 12 control persons by indirect immunofluorescent assay and ELISA. It was established that, primary infection by HHV-6B in Hodgkin's disease, its primary or recurrent infections in non-Hodgkin's lymphoma; primary or recurrent infection by HHV-6A in Hodgkin's disease, its recurrent infection in non-Hodgkin's lymphoma; recurrent infection by HHV-7 in Hodgkin's disease may contribute to the deterioration of clinical conditions. Probably, HHV-7 exerts its effects through activating HHV-6B. The simultaneous effects of HHV-7 and HHV-6A, and that of HHV-6B and HHV-6A seem to be independent. Our results supports the recent opinion that, the effect of these herpesviruses on the tumorous cells is exerted indirectly by altered mediators of the immune system.

Female↗
PubMed1998

[Chronic myeloproliferative diseases].

The chronic myeloproliferative syndromes are bone marrow stem cell disorders. An increase of cell counts of one or rather all three blood cell types is characteristic for these disorders. The most important diseases in this group are: chronic myelogenous/granulocytic leukemia, polycythemia rubra vera, osteomyelosclerosis or agnogenic myeloid metaplasia and essential thrombocythemia. The cells are normally differentiating in these diseases, while the control of cell dividing is abnormal and therefore the cells are produced and accumulated in excess. Splenomegaly is a common and characteristic clinical finding. The fibrotic or sclerotic transformation of the bone marrow can take place in all forms of the syndrome. Extramedullary haematopoiesis can occur in all of the above diseases, but it is most common in myelofibrosis/agnogenic myeloid metaplasia. In the last phase of the disease a terminal blastic crisis may terminate the course of chronic myeloproliferative diseases. The myeloproliferative disorders can be transformed in each other--the most common transformation is that of the polycythemia rubra vera into myelofibrosis. The greatest progress in the therapy of chronic myeloproliferative diseases is achieved in chronic myelogenous leukemia: bone marrow transplantation and interferon treatment (the latter also in essential thrombocythemia and polycythemia rubra vera) are routine modalities worldwide. A new drug, anagrelide is effective in the treatment of myeloproliferative thrombocytosis and thrombocythemia.

Chronic Disease↗
PubMed1997

Expression of syndecan-1 in human B cell chronic lymphocytic leukaemia.

Syndecan-1 is considered an important transmembrane proteoglycan in cell-microenvironment interactions, but its exact function in normal or in transformed B cells is still unknown. In this study, RNA was isolated from peripheral cells of chronic lymphocytic leukaemia (B-CLL) and 'normal', non-leukaemic patients, as controls. Reverse PCR showed no or very low syndecan-1 mRNA expression in controls, while in 11/13 B-CLL the circulating leukaemic cells expressed syndecan-1. Similar results were obtained for interleukin-1 beta (IL-1 beta) and interleukin-6 (IL-6). Furthermore, syndecan-1 protein was detected in the majority of circulating B-CLL cells by flow cytometry and immunocytochemistry using anti-syndecan-1 MAb. Control cells were practically negative. Further study is required to understand the biological significance of syndecan-1 on B-CLL cells.

Adult↗
PubMed1996

[Small intestinal leiomyoma causing gastrointestinal hemorrhage].

Leiomyomas are uncommon benign tumors of smooth muscle which may occur wherever smooth muscle is present. The small bowel is the most frequent site. Bleeding is the most common presenting symptom. Other symptoms, nausea, vomiting, abdominal pain are rare. In this article a 44 year-old male, presented, who was admitted with intermittent gastrointestinal bleeding in requence of jejunal leiomyoma with exulceration.

Adult↗
PubMed1996

Management of hemophilias--gene therapy to come?

BACKGROUND: Hemophilias are relatively common coagulopathies that require very careful management to prevent bleeding and related complications. OBJECTIVE: To review the current management of hemophilias, with an overview of gene therapy. SUMMARY: Although with the availability of highly purified and recombinant factor concentrates the replacement therapy of hemophilias has become safer and more effective, a therapeutic method providing continuous supply of clotting factors is desirable. Theoretically, this is possible, by transplanting organs, than can express antihemophilic proteins, but in most cases a less invasive method is preferable. Therefore the gene therapy would be revolutionary in treating hemophiliacs. CONCLUSION: The treatment of choice for hemophilias is the infusion of highly purified or recombinant factor products. Although still in experimental level, gene therapy for hemophilias is promising and may become the treatment of choice in these disorders.

Blood Transfusion↗
PubMed1991

Role of interferon in clinical practice.

Interferons are currently the most widely used biological response modifiers. They are of high clinical value in haematological malignancies (chronic myelogenous leukaemia, multiple myeloma, non-Hodgkin lymphoma), in solid tumours (malignant melanoma, hypernephroma, pancreas neoplasms, carcinoid tumours, Kaposi's sarcoma, glioma, in ovarium, cervix and bladder carcinoma, and in basalioma) and in infectious diseases (chronic hepatitis B, chronic non-A/non-B hepatitis, chronic delta hepatitis, AIDS, Papova virus and Rhinovirus infections, leishmaniasis, leprosy) and some other conditions. Although the mechanism of action of interferons has not been explained in every detail these agents are promising therapeutic means in a number of diseases.

Humans↗
PubMed1990

[The interleukins ].

Immune response is regulated by a number of soluble mediators. Interleukins belong to these mediators, as a well defined group of regulatory molecules. Interleukins are necessary for the differentiation and activation a T and B lymphocytes and other cells. They play an important role in inflammation: they cause fever, leukocytosis etc. Some interleukins are being used as therapeutic agents.

Adjuvants, Immunologic↗
PubMed1988

Serum type-III-procollagen and laminin concentration after interferon therapy in chronic myeloproliferative disorders.

Serum procollagen-III-peptide (P-III-P) and laminin P 1 (LP1) concentrations were measured in 24 patients with various chronic myeloproliferative diseases, viz., essential thrombocythaemia (ET), chronic myelogenous leukaemia (CML) and myelofibrosis (MF). The highest P-III-P values were found in myelofibrosis. As compared to the controls, the serum laminin concentration was higher in all there groups of patients. No significant difference was found between the groups of patients. After three months of alpha-IFN therapy the P-III-P values became significantly higher, the laminin concentration slightly decreased in all investigated patients. Longitudinal follow-up myeloproliferative patients should be carried out in order to prove whether P-III-P and laminin determinations provide a useful test to monitor the progression of the disease and the effect of the therapy.

Adult↗
PubMed1984

Experimental models for the study of hepatoprotection.

In the therapy of chronic liver diseases several drugs are currently used. This review summarizes the results of the authors in the therapy of chronic liver diseases with cyanidanol-3, as well as the beneficial effects of the new dihydroquinoline-type antioxidants in acute carbon tetrachloride induced and galactosamine induced liver lesions. In addition, the immunostimulant effects of Aicaphosphate is demonstrated in chronic active hepatitis.

Animals↗
PubMed1984

Effect of cyanidanol-3 on lysosomal enzyme activity of serum and granulocytes in chronic liver disease and active hepatitis.

The authors examined the effect in vivo and in vitro of Catergen (cyanidanol-3) on demonstrable lysosomal enzyme activity in the serum and granulocytes in liver diseases. Acid phosphatase, cathepsin-D and beta-glucuronidase were investigated. In the in vitro studies the direct effect of Catergen was observed by enzyme release. In chronic active hepatitis without treatment the lysosomal activity of the serum increases, the lysosomal activity of the granulocytes decreases and in vitro release increases. Under the effects of Catergen treatment the lysosomal activity of the serum decreases in comparison with initial values, approaching the lysosomal enzyme activity observed in healthy persons. Under in vitro conditions, lysosomal enzyme release from the granulocytes decreases in treated individuals and thus a higher lysosomal enzyme activity in the granulocytes is observed. Granulocytes separated from the blood of healthy persons were incubated with Catergen in isotonic and hypotonic media. In an isotonic medium the release did not change significantly. Under the effect of hypotension the release of lysosomal enzyme increased considerably. In vitro incubation with small doses of Catergen significantly inhibited the degree of release. On the basis of these data it may be supposed that Catergen has a stabilizing effect on the lysosomal membrane and is thus beneficial in the treatment of liver injuries (alcoholic, toxic, inflammatory) with lysosomal membrane alterations.

Acid Phosphatase↗
PubMed1982

Biochemical markers in carbon-tetrachloride-and galactosamine-induced acute liver injuries: the effects of dihydroquinoline-type antioxidants.

The pharmacological action and possible therapeutic uses of some recently developed synthetic, non-toxic dihydroquinoline-type antioxidants were studied. The effect of the lipid-soluble 6,6-methylene-bis (2,2,4-trimethyl-1,2-dihydroquinoline) (n = 1, 2 or 3) (MTDQ) on carbon-tetrachloride-induced acute liver injuries was investigated, and that of the water-soluble 6,6-methylene-bis (2,2-dimethyl-4-methansulphonic acid sodium-1,2-dihydroquinoline) (MDS) on galactosamine-induced acute liver injuries in CFLP mice (Lati, Hungary). MTDQ was found suitable for the prevention of acute CCl4-induced liver injuries and MDS for that of acute galactosamine-induced liver injuries. Disappearance or significant diminution of the morphological signs and lesions of lipid degeneration and centro-lobular liver necrosis, decrease of serum GOT activities, and also inflammatory changes induced by galactosamine were observed.

Animals↗
PubMed1982

Acute carbon tetrachloride induced lysosomal membrane damage and the membrane protecting effect of a new dihydroquinoline-type antioxidant.

The authors examined the damage of lysosomal membrane caused by acute CCl4 intoxication by in vitro methods. They measured the acid phosphatase as well as beta-glucuronidase enzyme levels and determined the rate of release of these two enzymes. The in vivo changes in enzyme activity were extrapolated from the in vitro results. The CCl4 causes a significant increase in the permeability and rigidity of the lysosomal membrane. By oral and/or intraperitoneal administration of MTDQ the state of permeability can be improved or even corrected. On the basis of their results, the authors conclude that the lysosomal damage caused by CCl4 is mediated by peroxidation of lipids and the lysosomal membrane can be stabilised by MTDQ.

Animals↗