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Biomedical subjects

L Skoog

Publications and source records attributed to L Skoog.

191 records · Page 11Linked to original sources
PubMedDate not supplied

Immunocytochemical analysis and cytomorphologic diagnosis on fine needle aspirates of lymphoproliferative disease.

Fine needle aspirates were used for the cytologic and immunologic analysis of 21 cases of lymphoproliferative disorders. Immunocytochemical studies performed on Cytospin preparations confirmed the cytomorphologic diagnosis in 19 cases. In one case, the morphology of both aspirates and surgically obtained material showed a reactive pattern while immunologic analyses were inconclusive on both types of material. Immunocytochemistry on tumor material obtained by fine needle aspirations was in agreement with immunohistochemistry on surgical biopsies in 15 of 16 patients with malignant lymphomas. We conclude that immunocytochemical studies performed on Cytospin material in conjunction with the cytologic diagnosis will lead to an increase in diagnostic accuracy as well as providing a means for subclassification of neoplastic lymphoid cells. Moreover, this technique appears to give results comparable to those obtained by histopathologic and immunohistochemical analysis on surgically removed lymph nodes.

Adult↗
PubMedDate not supplied

Estrogen receptor content in primary breast carcinomas and their metastases.

The estrogen receptor content was measured in 25 primary human mammary carcinomas. These values were compared to those in metastases which were detected between 0-108 months after mastectomy. There was a good correlation between the receptor level in the primary tumor and in its metastasis. This finding does not support the hypothesis that metastases in general represent a selection of poorly differentiated receptor-poor cell clones from the primary tumors.

Adult↗
PubMedDate not supplied

Detection of TdT in AML blasts by immunological and biochemical techniques.

The cellular level of terminal deoxynucleotidyl transferase (TdT) was measured in blasts from patients with AML. Parallel determinations were made using an immunoassay and polymerizing reaction. There was a good correlation between the two techniques (r = 0.92) and detectable levels of the enzyme was found in approximately 50% of the cases. The TdT levels found in AML blasts were 1/10 - 1/100 of that reported for cells of lymphoid origin.

DNA Nucleotidylexotransferase↗
PubMedDate not supplied

Identification of transforming growth factor alpha in human primary breast carcinomas.

Primary human mammary carcinomas were found to contain an acid stable polypeptide which bound to the receptor for epidermal growth factor (EGF). This polypeptide which was antigenetically different from EGF and thus identified as Tumor Growth Factor (TGF) alpha, could be identified in 25% of the tumors. Among these tumors 40% contained measurable levels of the EGF receptor. Thus these tumors exhibit the molecular prerequisite for autocrine growth stimulation.

Breast Neoplasms↗
PubMedDate not supplied

Immunohistochemical analysis of estrogen receptors in cells obtained by fine needle aspiration from human mammary carcinomas.

An immunocytochemical assay for the estrogen receptor has been used to analyze the receptor content in tumor cells obtained by fine needle aspiration from human breast carcinomas. The results were compared with receptor determinations on cryostat sections of freshly frozen tumors and by biochemical technique based on radiolabelled ligand binding. The staining intensity and fraction of positive cells were comparable in cryostat sections and tumor cell specimens obtained by fine needle aspiration. Moreover, there was a good correlation between the semiquantitative receptor level estimated by the immunoperoxidase technique and the biochemical assay. These results show that tumor cell specimens obtained by fine needle aspirates can be used for estrogen receptor analysis by the immunocytochemical technique.

Adult↗
PubMedDate not supplied

Prognostic significance of the receptor for epidermal growth factor in human mammary carcinomas.

125I-EGF binding technique was used to demonstrate high affinity receptor binding for epidermal growth factor (EGF-R) in 72 human mammary carcinomas. 27% of the tumors were EGF-R positive and the presence of this receptor was correlated with receptor levels for estradiol, DNA-pattern, proliferative index, thymidine kinase, tumor size, number of lymph node metastases and 6-year relapse probability. Our results confirm previous reports of an inverse relation between the cellular content of EGF-R and ER. In addition, we could associate EGF-R positivity with an aneuploid DNA-pattern and an increased growth rate, as measured by proliferative index. No correlation was found between EGF-R positivity in the primary tumor and presence of axillary lymph node metastasis at the time of operation. The 6-year relapse rate was somewhat higher for patients whose primary tumors were EGF-R positive. Moreover patients who had lymph node metastases at the time of operation and EGF-R positive tumors experienced a significantly lower 6-year disease free survival rate as compared to those who were node negative and had receptor negative tumors. It remains to be shown whether EGF-R alone can be used as an independent prognostic factor.

Adult↗
PubMedDate not supplied

Fine needle aspiration cytology of tubular adenoma of the breast. A report of two cases.

During the routine practice of fine needle aspiration of the breast, two tubular adenomas were encountered. The patients were 46 and 69 years of age. In one case the appearances indicated a benign epithelial proliferative lesion, while in the other, epithelial atypia necessitated surgical excision. The differential diagnoses included other benign epithelial proliferative lesions and carcinoma. To avoid inappropriate surgery, the cytomorphologic spectrum of benign epithelial proliferative lesions, including tubular adenoma, must be recognized.

Adenoma↗