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Biomedical subjects

L Schindler

Publications and source records attributed to L Schindler.

24 records · Page 2Linked to original sources
PubMed1982

Activation of natural killer cells and induction of interferon after injection of mouse hepatitis virus type 3 in mice.

High levels of natural killer (NK) cell activity and high titers of interferon were observed in the peritoneal exudate of C57BL/6 mice 20 to 50 h after injection of mouse hepatitis virus type 3 (MHV3) but not during the first 10 h after infection. C57BL/6 mice were susceptible to MHV3 infection and showed high titers of MHV3 in the peritoneal exudate 48 h after infection. A/J mice, in contrast, were resistant to the dose that killed 100% of the C57BL/6 mice (10 macrophage-infecting doses) and showed considerably lower virus titers than those shown by C57BL/6 mice. NK cell activity and interferon titers were significantly lower in the peritoneal exudate of A/J mice than in that of C57BL/6 mice. Serum interferon titers were also lower in A/J mice. Thus, our data show an inverse relationship between resistance and the levels of these two parameters. The data suggest that, in contrast to the situation observed with certain herpesviruses, interferon and NK cells may not be of overwhelming importance in the defense of mice against MHV3.

Animals↗
PubMed1981

Protection of mice against mouse hepatitis virus by Corynebacterium parvum.

C57BL/6 mice that are highly susceptible to infection with mouse hepatitis virus type 3 were protected against intraperitoneal viral infection by simultaneous intraperitoneal injection of Corynebacterium parvum. No protection was observed when C. parvum was given intravenously or when it was injected intraperitoneally 3 days before viral infection. Protective effects were, however, consistently found when C. parvum was given 2 h before or 2 h after viral infection. Activity was seen only against 10 50% lethal doses and not against 100 50% lethal doses. C. parvum also caused a significant decrease of virus type 3. These data suggest a direct effect of C. parvum on virus-susceptible cells. Injection of C. parvum in mice caused activation of natural killer (NK) cells and of interferon production. However, these two effects were equally demonstrable at high and low doses of C. parvum, whereas protection against mouse hepatitis virus type 3 was not demonstrable at low doses of C. parvum. Thus, antiviral protection may be dissociated from activation of NK cells and induction of interferon.

Animals↗
PubMed1975

Inhibitory postsynaptic actions of taurine, GABA and other amino acids on motoneurons of the isolated frog spinal cord.

The actions of glycine, GABA, alpha-alanine, beta-alanine and taurine were studied by intracellular recordings from lumbar motoneurons of the isolated spinal cord of the frog. All amino acids tested produced a reduction in the amplitude of postsynaptic potentials, a blockade of the antidromic action potential and an increase of membrane conductance. Furthermore, membrane polarizations occurred, which were always in the same direction as the IPSP. All these effects indicate a postsynaptic inhibitory action of these amino acids. When the relative strength of different amino acids was compared, taurine had the strongest inhibitory potency, followed by beta-alanine, alpha-alanine, GABA and glycine. Topically applied strychnine and picrotoxin induced different changes of post-synaptic potentials, indicating that distinct inhibitory systems might be influenced by these two convulsants. Interactions with amino acids showed that picrotoxin seletively diminished the postsymaptic actions of GABA, while strychnine reduced the effects of taurine, glycine, alpha- and beta-alanine. But differences in the susceptibility of these amino acid actions to strychnine could be detected: the action of taurine was more sensitively blocked by strychnine compared with glycine, alpha- and beta-alanine. With regard to these results the importance of taurine and GABA as transmitters of postsynaptic inhibition on motoneurons in the spinal cord of the frog is discussed.

Alanine↗