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Biomedical subjects

L Samuelson

Publications and source records attributed to L Samuelson.

28 records · Page 2Linked to original sources
PubMed1987

A simple technique for increased sensitivity in immunoelectrophoresis by the use of antiserum against IgG.

Precipitates in two-dimensional immunoelectrophoresis (2D-IE) plates can be amplified by the application of an antiserum against IgG after the second dimension run. Thereby, the sensitivity of the IE method is increased; faint precipitates and antigen-antibody reactions close to but below the level of forming recognizable precipitates are made visible. The advantage of the method was demonstrated for a multilinear Mycobacterium phlei system.

Animals↗
PubMed1986

A thin gel layer technique for improved resolution in two-dimensional immunoelectrophoresis.

A modification of the two-dimensional (crossed) immunoelectrophoresis (2D-IE) method characterized by the employment of a thin gel layer (0.3 mm) was developed. A rectangular Millipore filter dehydrates an area of the gel, thereby forming a basin which is charged with antiserum by means of a special applicator. This 2D-IE technique was compared with the techniques by Weeke [Scand. J. Immunol. 2: suppl. 1, pp. 47-56, 1973] and by Lebecq et al. [J. immunol. Methods 66: 219-226, 1984] using a model system comprising an antigen preparation from Mycobacterium intracellulare and a corresponding antiserum. Analyses by the technique described resulted in a distinct, multilinear precipitation pattern with dense and sharp precipitates contrasting well against the background.

Antigen-Antibody Reactions↗