Biomedical subjects
Publications and source records attributed to L Samochowiec.
Explore the source record for details and available documents.
Explore the source record for details and available documents.
Explore the source record for details and available documents.
The influence of atherogenic diet and "essential" phospholipids (EPL) both on exploratory activity and catecholamine content in the brain of rats has been examined. The atherogenic diet caused a decrease of the animals' exploratory activity as well as a diminution of catecholamines. It was observed that these changes correlated. Prophylactic administration of EPL is capable of preventing such changes. Therapeutic administration of EPL increased exploratory activity and catecholamines in the atherogenic group which subsequently received the basic laboratory diet.
A total of 45 workers, who had been chronically exposed to a potentially hepatotoxic xylene-toluene-benzene mixture in the atmosphere, were treated for 3 months with 'essential' phospholipids. Routine biochemical tests and radioisotope investigations to assess liver damage were carried out both at baseline and after treatment. The liver:spleen index was increased in 71% of subjects and the mean isotope transit time in the liver was reduced in 90% of the patients after treatment. The biochemical parameters remained within the normal range throughout the treatment period. The results suggest that 'essential' phospholipids may be of value in the treatment of liver damage that has arisen as a result of chronic exposure to organic solvents.
Three (R,S)-1-N-(theophyllinyl-7'-ethyl)-amino-2-propanol derivatives were obtained as soluble hydrochlorides and tested in four models of experimental arrhythmia. Two compounds 1a and 2 have shown antiarrhythmic properties and very low toxicity.
The effect of mestranol and chlormadinone, the hormones contained in oral contraceptives: on lipid metabolism, some hemostatic parameters and on morphology of aorta, liver and heart was tested after separate or combined administration to mongrel female rabbits fed on a normal or high-fat diet (HFD). HFD administration led to typical atheromatous changes in the aortal intima. The hormones given together with HFD attenuated the augmentation of lipid serum level and the extent of atheromatous plaques in the intima. The percent content of alpha-lipoproteins was significantly lower in groups receiving HFD with or without the hormones than in the controls, but it was higher than in controls in the rabbits receiving the hormones alone. Chlormadinone given together with HFD potentiated atheromatous aortal changes, which occurred not only in the intima but also in the media. Mestranol given together with HFD significantly counteracted the development of atheromatous changes. Female rabbits receiving mestranol and chlormadinone displayed a significant increase in fibrinogen content, while those receiving mestranol showed an increased time of fibrinolysis. HFD produced a hepatic damage, which was augmented in rabbits concomitantly receiving the hormones. The hormones alone also displayed some, a less pronounced hepatotoxic action.
In male Wistar rats the protective effect of Padma 28 against changes induced by prolonged treatment with ethyl alcohol was investigated. Exposure of the animals to prolonged ethyl alcohol consumption caused a significant increase in AspAT (by 32%), A1AT (by 50%) and alkaline phosphatase (by 372%) activities as well as an increase in the levels of bilirubin (by 98%) and lipids in the blood serum and lipids in the liver homogenate. Padma 28 administration was found to exert a protective action against these changes: a significant reduction of blood total lipid, triacylglycerol, total cholesterol and bilirubin concentrations as well as in the activity of the liver enzymes was observed.
Radioimmunoassay of serum thyroid hormones (T3 and T4), testosterone, estradiol, insulin and cortisol of rabbits fed on a high-fat diet (coconut oil, cholesterol, cholic acid) for three months and developing experimental atherosclerosis (confirmed by biochemical tests and morphological changes in the aorta) have revealed an increase in the content of estradiol and insulin, and a decline in the level of thyroid hormones and testosterone.
A new derivative of diprophylline--[2-hydroxy-3(theophylline-7-ol)] propyl ester 4-benzyloxybenzoic acid was shown to decrease the content of cholesterol and of atherogenic lipoproteins in blood serum of hyperlipidemic guinea pigs. In blood serum of rabbits the new substance studied decreased cholesterol, free fatty acids and malonic dialdehyde concentrations.
A series of theophyllinyl-7'-ethyl derivatives of (RS), (R)(-) and (S)(+) 2-aminobutanol were synthesized and tested in various models of experimental arrhythmia for their preventive and curative properties; (R)(-) 2-N-theophyllinyl-7'-ethyl amino-1-butanol (1a) and (R)(-) theophyllinyl-7'-ethyl-2-N-methylamino-1-butanol (3a) hydrochlorides showed interesting antiarrhythmic properties. The levorotatory compounds of R configuration showed stronger protective and also therapeutic effect in different experimental arrhythmias.
To assess the hypolipemic and antiatherosclerotic activity of diprophyllinyl-4-benzyloxybenzoate guinea-pigs were kept on a high-lipid diet alone or with the tested compound for 21 days, and rabbits--for 3 months. It was found that diprophyllinyl-4-benzyloxybenzoate displays a distinct hypolipemic activity, particularly with respect to cholesterol, both in guinea-pigs and rabbits. It also depresses the cholesterol and the trigliceride content in liver homogenates and shows a tendency to normalize the low-density-lipoprotein fraction in guinea-pigs, as well as cholesterol of the high-density lipoprotein fraction, and malon dialdehyde in rabbits. The area of intima covered with atheromatous plaques was smaller in rabbits receiving the high-lipid diet with diprophyllinyl-4-benzyloxybenzoate than in those receiving that diet alone.
In order to determine the efficacy of new compounds--an amino acid derivative of clofibric acid (WKLB-5), and a theophylline monoester of nicotinic acid (ME1)--a high fat diet (HFD) was applied to rabbits for 3 months, with or without the above mentioned compounds. The HFO included coconut oil, cholesterol and cholic acid. A substantial hypolipemic activity of those compounds was demonstrated; however, the studied lipid fractions did not undergo normalization, except FFA whose level after ME1 administration was distinctly lower than in control animals. Sudanophilic changes were found on 83% of surface of the interior membrane of aorta in rabbits kept on the HFD only, on 35% of surface--in rabbits treated with the HFD + WKLB-5, and on 75% of surface--in rabbits treated with the HFD + ME1. Thus in those animals no parallelism between the hypolipemic and antiatherosclerotic activities was observed.
The effect of colchicine on the course of experimental atherosclerosis was investigated in rabbits receiving for 3 months a high-lipid diet (HLD) in addition to the basal diet. Colchicine was given in a dose of 0.2 mg/kg ip twice a week. An evident antiatherosclerotic action of colchicine was demonstrated both by macroscopic and microscopic investigation of the aorta. The level of blood total lipids was significantly depressed, and the level of triglycerides was back to normal. The content of total lipids in liver homogenates was similar to that in the controls. A significant decline in the fibrinogen concentration in the blood of rabbits receiving colchicine was also found.
Rabbits, guinea pigs and rats received for 3 months a high lipid diet containing coconut oil and cholesterol, separately or in combination. The blood lipid content was assayed. Lipoproteins were separated into fractions by agarose electrophoresis. The aorta, heart and liver were investigated macro- and microscopically. The most pronounced changes in lipid metabolism appeared as a consequence of combined administration of coconut oil and cholesterol; they were most expressed in rabbits, less in guinea pigs, and most weakly in rats. Typical atherosclerotic lesions were observed only in rabbit's aorta. They were accompanied by a marked depression of the percentage content of alpha-lipoproteins in blood serum.
Explore the source record for details and available documents.
Explore the source record for details and available documents.
The effect of intramuscular injection of atropine and papaverine on the pharmacokinetics of a single oral dose of paracetamol in healthy men was investigated. The open two-compartment model was applied and the calculations were performed using a program for the Hewlett-Packard 9830 B system. An increase of the extent of bioavailability of paracetamol was observed after the atropine administration, however the absorption of the drug was delayed. The administration of papaverine did not change the AUC and Cmax, but tmax was significantly longer.