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Biomedical subjects

L Salvaggio

Publications and source records attributed to L Salvaggio.

34 records · Page 2Linked to original sources
PubMed1990

Yersinia enterocolitica and related species isolated in the Pesaro and Urbino area (Italy) from 1981 to 1986.

A total of 23 strains of yersinias, Y. enterocolitica (17), Y. frederiksenii (5) and Y. intermedia (1) characterized according to bio-serogroup and phage type, were isolated from human, animal and environmental samples during a 5-year period. It appears that in the Pesaro-Urbino area Yersinia spp. are infrequent and the strains of Y. enterocolitica belong to environmental and rarely to human pathogenic bioserogroups.

Adolescent↗
PubMed1990

Role of free radicals produced during the metabolism of mitomycin C in Escherichia coli inactivation.

1. The primary objective of this study was to assess whether reactive oxygen species produced during the metabolism of mitomycin C are responsible for, or contribute to, the induction of the cytotoxic response. 2. Escherichia coli wild-type and superoxide dismutase mutants were grown either anoxically or euoxically, in K medium or minimal glucose medium, and treated with mitomycin C in K medium or M9 salts. The cytotoxic response did not vary significantly between the wild-type and the SOD mutants. 3. The toxicity of mitomycin C was not affected by the hydroxyl radical scavengers, dimethyl sulphoxide or ethanol, whereas protection was afforded by thiourea. 4. It is concluded that oxygen radical species do not play a significant role in mediating mitomycin C inactivation of normally growing bacteria.

Dimethyl Sulfoxide↗
PubMed1989

[Antibodies against the 15 amino acid sequence 599-613 of HIV-1 glycoprotein 41 in subjects at risk of HIV infection].

A peptide (called A-15) composed of 15 amino acids of the gp41 (from position 599 to position 613 of the sequence encoded by the env gene) of HIV-1 has been used as an antigen to search for antibodies in 347 sera of at-risk for HIV-1 infection subjects. The purpose was of comparing the prevalence of these antibodies with that of HIV-1 total EIA antibodies and HIV-1 immunoblotting antibodies. Assuming immunoblotting test as reference test for detecting a HIV-1 infection, the antibodies against peptide A-15 show the same sensitivity and specificity when compared with other HIV-1 total EIA antibodies tests.

Amino Acid Sequence↗
PubMed1989

Molecular mechanisms of hydrogen peroxide cytotoxicity.

The molecular mechanisms of H2O2 toxicity have been investigated in both mammalian or bacterial cells. DNA breakage mediates cytotoxicity by low concentrations of H2O2 in mammalian cells, but DNA lesions do not appear as a direct consequence of the action of the hydroxyl radical; rather, these radicals may disturb intracellular Ca2+ homeostasis, which results in secondary reactions ultimately leading to DNA strand breakage and cytotoxicity. Studies that have used Escherichia coli (E. coli) as a cellular system have indicated that the two modes of killing detectable in cells exposed to increasing concentrations of H2O2 are mediated by different radical species. Mode-one killing seems to be produced by the superoxide anion whereas mode-two killing seems to be a consequence of the hydroxyl radical attack.

Animals↗
PubMed1988

[Normal spirometric values in the Sicilian population].

Principal spirometric parameters have been measured in a sample group of 1641 male subjects. Due to the significant difference in values often found when using the standards proposed by CECA (1983) (particularly in the medico-legal field) in order to obtain reference values as close as possible to the local norm the chosen subjects are clinically and functionally healthy and come from different parts of Sicily. The regression plots obtained using three independent variables (age, weight, height) other than demonstrating a good affinity with the original sample and an elevated predictivity level, underline the difference between obtained reference values and European ones (higher for VC and FEV 1; lower for FRC and RV) even though evolutive trend and ageing overlap. The above mentioned difference, found also for some parameters (VC and FEV 1) using normal Italian standards can be explained; not only by different evaluations of normal conditions, respiratory risk, smoking etc., but also by the existence of a different ethnic constitution which influences in various ways the ventilatory parameters.

Adult↗
PubMed1987

The effect of temperature or anoxia on Escherichia coli killing induced by hydrogen peroxide.

The cytotoxicity of hydrogen peroxide in Escherichia coli was investigated after various conditions of drug exposure. Two modes of killing were detected following a 15-min challenge with H2O2 under either aerated or anoxic conditions. Mode one killing occurred at levels below 2.5 mM and mode two killing at concentrations higher than 10 mM. Whereas mode one killing was similar at the two conditions of drug exposure, mode two lethality differed in that aerated cells were more sensitive than anoxic cells. Independently of O2 tension the hydroxyl radical scavenger, thiourea, prevented mode two but not mode one killing by H2O2. Cells treated with the drug at ice temperature did not display mode one killing and mode two lethality occurred only at very high concentrations. We suggest that hydroxyl radicals mediate mode two but not mode one killing by H2O2.

Escherichia coli↗