Search PubMed⌕ Search

Biomedical subjects

L S Strochkova

Publications and source records attributed to L S Strochkova.

At least 19 recordsLinked to original sources
PubMed1990

[Hyposelenoses].

Literature on the various manifestations of the selenium deficiency in humans and animals is presented. Mechanisms of the biological effects of selenium brought about by glutathione peroxidase are considered. Biogeochemical anomalies, parenteral nutrition, malabsorption syndromes as conditions for the development of hyposelenosis are characterized as well as their manifestations (white-muscular disease, myopathies, cardiomyopathies, Keshan disease, Kashin-Beck disease). Selenium deficiency facilitates the development of the ischemic heart disease, myocardium infarction, certain types of malignancy and so on. The attention is drawn to the link of the selenium deficiency with imbalance of other trace elements.

Animals↗
PubMed1988

[Cellular homeostasis and trace elements].

The authors consider the relevance of essential and toxic elements to the maintenance and derangement of cellular homeostasis, review different routes of their introduction in and release from varying cell types as well as relationships with specific ligands, distribution by intracellular pools. Presented are also morphological data on trace elements accumulation in cell, organs and compartments. A number of trace elements contributed to mutagenesis and carcinogenesis while the others demonstrate anticarcinogenic and antiblastic action.

Animals↗
PubMed1986

[Effect of sodium selenite on tumor cells in culture].

Morphofunctional changes in HeLa cell culture following treatment with various concentrations of selenium ion during 2, 4 and 24 hours are described. Variations in mitotic index, duration of separate mitotic stages and a profile of pathologic mitoses were established. Inhibition of cell entry into the S-phase of mitotic cycle and modifications of RNA and protein synthesis after treatment with 0.1 and 0.25 ppm of the trace element was shown autoradiographically. Increased destruction of cell monolayer following contact with selenium was demonstrated by light, scanning and transmission electron microscopy. It is suggested that selenium exerts a toxic effect on tumor cells although this mechanism is not yet clear.

HeLa Cells↗
PubMed1985

[Changes in the protein-synthesizing system of a HeLa cell culture exposed to a number of trace elements].

The authors investigated the cytopathic action of maximal allowable concentrations (MAC) of zinc, nickel, cobalt, cadmium and fluorine on cell culture. The most significant changes in RNA synthesis were noticed after exposure to zinc MAC. After exposure to the MAC of zinc, nickel and fluorine considerable modifications of protein synthesis were recorded. It was established that the content of rRNA increased after incubation with all trace elements under study for 24 hours. The amount of protein experienced significant changes after nickel introduction into the incubation medium. It is concluded that exposure to some of trace elements entails considerable changes in cell metabolism.

Autoradiography↗
PubMed1984

[Morphological and metabolic changes in a HeLa cell culture exposed to fluorine].

Fluoride in subtoxic (1.5 mcg/ml) and toxic (12 mcg/ml) concentrations induced characteristic changes in the mitotic regime of HeLa cell culture. Fluctuations of the mitotic index, variations in the duration of division phases and in the spectrum of pathologic mitosis were noticed. It was shown that fluoride inhibited the genome reduplication. In lower concentrations the halogen produced the most expressed effect on transcription and translation, which corresponds to a so called paradoxial action of this trace element. Definite shifts in the ultrastructure of the cell surface and cell organelles were registered, which confirms a connection between morphological and functional changes in the culture during incubation with fluoride.

Autoradiography↗
PubMed1983

[Principles for classification of diseases of a biogeochemical nature].

The principles of working classification of human pathology associated with excess, deficiency and dysbalance of trace elements are presented on the basis of many-year analysis of the literature and own experimental and clinico-morphological observations. According to the most important variants of human contact with the environment abnormal with regard to the content of trace elements, the following types of diseases are distinguished: (1) natural, (2) technogenic, and (3) iatrogenic. In their turn, technogenic diseases are subdivided into industrial, neighbouring, transgressive. Among these diseases, monomicroelementoses and polymicroelementoses should be distinguished. The former include fluorosis, arsenosis, berilliosis, cadmium nephropathy, cobalt myocardiopathy, etc., the latter anemias of biogeochemical nature, endemic goiter, tooth caries, urolithiasis, selenium-deficient myocardiopathy, and others.

Aluminum↗
PubMed1981

[Dynamics of protein synthesis in several organs of mice with fluorosis].

Proceeding from the time course of 3H-leucine incorporation during chronic fluoride administration the mouse organs were distributed conventionally into three groups. The first group included the organs which did not manifest significant alterations in protein synthesis. On the contrary, the second group of the organs manifested pronounced oscillations in the synthetic activity during poisoning. However, the majority of the organs studied (the third group) was characterized by a sharp inhibition of isotope incorporation.

Animals↗
PubMed1980

[Dynamics of incorporation of 3H-actinomycin D into synchronized Chinese hamster cells].

Dynamics of 3H-actinomycin D (3H-AMD) incorporation has been studied in the Chinese hamster synchronized cell culture (line 237) during different cell cycle phases. Intensive isotope inclusions were seen in cells during interphase: in the beginning of G1 period, in the early S period, and in the late S period, during the cell passage to G2 stage. The authors suggest that the binding of 3H-AMD by cells may be due not only to conformational changes of DNA structure, but also due to the variation in cell membrane penetration throughout the mitotic cycle.

Animals↗
PubMed1979

[Effect of the inhibition of a synthesis processes in the S-period on the subsequent course of mitosis in a synchronized Chinese hamster cell culture].

The transcription of all nuclear RNA and rRNA was inhibited in the synchronized Chinese hamster cell culture by actinomycin D and protein synthesis was suppressed by pyromycin during the first and the second parts of the S period of the mitotic cycle. A decrease of the mitotic cell activity was revealed during the first and second waves of cell proliferation after the synchronization irrespective of the time of the antibiotic administration. At the same time there was noticed an increase of such pathological mitoses as C-mitoses and chromosome scattering in the metaphase, whereas suppression of rRNA synthesis determined the delay of cell going out of mitosis (an increase of the anaphase and telophase cell portion) mostly correlated with such type of pathological mitoses as chromatid and chromosome bridges. The protein synthesis depression during the first half of the S-period failed to influence the mitotic regimen of the cell culture during the first, nearest division, but suppressed considerably the cell proliferation during the distant proliferation wave. It is suggested that the cells entered the S-period having in store the proteins for initiation and supporting the genom reduplication at least during the first stage of this process.

Animals↗
PubMed1979

[Pathology of mitosis after cellular recovery from a metaphase block].

The experiments in Chinese hamster fibroblast-like cells have shown that the c-mitosis inducing agents (colchicine, colcemid and low temperature) produce evident stathmokinetic and radiomimetic effects. By the reversibility moment of the former the latter becomes apparent, manifesting in bridge accumulation. This form of pathology is likely to be caused by the disturbance of the cellular nucleoprotein metabolism in c-mitosis.

Animals↗
PubMed1979

[DNA content and the endometrial cellular mitotic activity in the phase of menstrual bleeding].

Endometrium obtained during menses from 46 healthy women in reproductive age was investigated morphologically and cytospectrophotometrically in order to solve the problem on the source of the cells reepithelizing the uterine mucous membrane after desquamation. It was stated that desquamation takes place not in the whole functional layer of endometrium, some mucous fragments, covered with persisting luminal epithelium, are always preserved. During endometrial regeneration the cells of the luminal and glandular epithelia and those of endometrial stroma are predominantly diploid. The amount of premitotic cells in population is so small that they cannot secure any intensive cellular proliferation. Mitogenesis in endometrium is stimulated only after a complete restoration of the epithelial layer. It is suggested that persisting luminal epithelium is the source of cells for reepithelization; they migrate towards endometrial "wounds" and repair defects in the uterine mucosa during the regeneration phase.

Adult↗
PubMed1978

[Effect of inhibitors of RNA and protein synthesis on the course of mitosis in a synchronized culture of Chinese hamster cells].

The synthesis period of various types of RNA and proteins during the interphase related to the immediate after synchronization mitosis was investigated on teh synchronized culture of Chinese hamster cells with inhibitors. Analysis of MI and forms of mitosis pathology indicated the proteins functionally related to the cell division to be mainly synthesized during the second half of the interphase. The most important action of pyromycin during this time is the induction of C-mitosis on account of suppression of proteins synthesis responsible for the spiralization of chromosomes during the prophase. Inhibition of the r RNA transcription at any stage influence the cytotomy and reconstruction of daughter nuclei, this being accompanied by the delay of the cell exit from the mitosis. In difference from pyromycin, a high dose of AMD as an inhibitor of the whole cell RNA synthesis was most effective during the first half of the interphase. It is suggested that during this period at least three kinds of RNA are synthesized which are functionally related to the immediate mitosis in a different way: RNA for genome reduplication, RNA for the tubulin synthesis, and RNA which is the division spindle component.

Animals↗
PubMed1978

[Remote changes in the course of mitosis in synchronized cultures of Chinese hamster cells following inhibition of RNA and protein synthesis].

The effect of inhibition of the synthesis of some types of RNA and proteins was determined in the synchronized culture of Chinese hamster cells at various stages of the interphase on the course of remote mitosis. Analysis of MI and some forms of pathological cell division showed that the action of different doses of AMD and pyromycin during the first part of the interphase provoked an identical effect--C-mitosis at the immediate and remote waves of cell division. Suppression of the synthesis of whole cell RNA and proteins during the second half of the interphase was accompanied by a metaphase cell delay with scattering chromosomes, this indicating derangement of the synthesis of the division spindle component. It is suggested that proteins (tubulins) and RNA participate in the organization of the division spindle during remote mitosis as a "reserve pool".

Animals↗
PubMed1978

[Characteristics of mitosis pathology in a Chinese hamster cell culture with disorders of the protein-synthesizing system].

Disturbance of protein synthesis with puromycin and transcription of chromosomal and ribosomal RNA with actinomycin D was followed by marked changes in the normal course of mitosis. There was noted an increase in number of colchicin-like mitoses (C-mitoses), and particularly, sometimes, fragmentation of their cytoplasm with the formation of cluster-like structures. It is suggested that development of C-mitosis was connected not only with destruction in the mitotic apparatus formation system, but also with the block of synthesis of one of the chromosomal proteins, stabilizing DNA strands spiralization. Another form of pathological mitosis occurring under conditions of suppression of the metabolic processes ("hollow metaphase plate") was linked not only with the chromosomal alterations. Selective suppression of the ribosomal RNA transcription led to an evident anaphase delay and to coupling of telomere regions of chromosomes. This phenomenon is apparently associated with destruction of the "protective cover" of chromosomes, formed by RNA of the disintegrating nucleoli and RNA of perichromatin granules.

Animals↗