Search PubMed⌕ Search

Biomedical subjects

L S Iakovleva

Publications and source records attributed to L S Iakovleva.

At least 37 records · Page 2Linked to original sources
PubMedDate not supplied

[Search for HHV-8 associated diseases reservoir and spread paths of HHV-8 in Russia].

Summarized in the paper are study results of human herpesvirus type 8 (HHV-8) and of its association with Kaposi's sarcoma (KS). The data obtained denotes that the share of individuals producing the antibodies to HHV-8 in a majority of studied patients was low and ranged form 0 to 5.5%, which is indicative of a low degree of the virus spread in population. At the same time, a high share of persons with antibodies to HHV-8 was detected among HIV-infected homosexuals (71.4%), kidney recipients (26.0%) and among AIDS-KS patients (78.6%). It was also unexpectedly high among patients with T- and B-cell lymphomas (50%), encephalopathy (27.3%) and with stomach cancer (41.8%): the appropriate parameters were 7-12-fold higher versus healthy subjects. The HHV-8 markers, i.e. virus specific antibodies and/or nucleotide sequences of the virus, were detected in blood serum and ejaculate of a significant number of patients with different pathologies of the prostate. Such detection of viral markers in the above categories of patients is suggestive of that sexual contacts with such patients are decisive for the HHV-8 spread in population.

Acquired Immunodeficiency Syndrome↗
PubMedDate not supplied

[LMP1 gene variants of Epstein-Barr virus in patients with some lymphoproliferative malignancies].

The samples of tumor biopsy, blood, and saliva from 10 patients with Hodgkin's disease, 10 patients with non-Hodgkin's lymphoma, and the blood samples of 20 donors were tested by polymerase chain reaction (PCR) for standard (wild) B95-8 and Cao-like (deleted) variants of the LMP1 gene. The paraffin sections of most PCR-tested tumors were also investigated by immunohistochemistry using the monoclonal antibodies S12 or 7D7 to detect the expression of the standard or Cao-like variants of LMP1 protein, respectively. It is suggested that Eptein-Barr virus (EBV) that contains the above deletion is not crucial for the development of the study lymphoproliferative malignancies. The fact that in some cases there is the Cao-like variant of LMP1 in the tumor biopsy specimen and its standard variant LMP1-B95-8 in the biological fluids of the same patient is very likely to suggest that the patient is infected with both types of the virus or there is genetic mutation(s) of EBV during viral carcinogenesis preceding or accompanying the development of a tumor.

Biopsy↗
PubMedDate not supplied

[A study of sera positive and doubtful for antibodies to the human immunodeficiency virus for the presence of HTLV-I-specific antibodies].

The results of examinations for the presence of antibody to HTLV-I of 205 sera which were positive or indefinite (doubtful) with regard to HIV are presented. No evidence indicating simultaneous infection with HIV and HTLV-I viruses in 18 HIV-positive subjects was obtained. Antibodies to gag-proteins p19 and p28 HTLV-I were detected in one serum showing primary reactivity in screening for HIV antibodies. Problems of HIV and HTLV-I occurrence and sensitivity of screening test systems are discussed.

Agglutination Tests↗
PubMedDate not supplied

[Mechanism of postvaccinal immunity in Marek's disease].

The apathogenic variant No. 38 of Marek's disease virus, Kekava strain (MDV-Kekava), at the level of the 16th and 45th passages in vitro made chickens resistant to Marek's disease when inoculated 14 days before the infection of the chickens with the pathogenic variant No. 55 of MDV-Kekava. A simultaneous administration of both variants did not protect the animals against the disease. The occurrence in variants of MDV-Kekava of genetic markets manifest upon virus passages in chick embryo fibroblast (CEF) cultures provided an opportunity to study the interactions between them in chickens, using the CEF culture for virus isolation. The results of virus isolation from the blood cells of vaccinated chickens showed interference to occur in chickens between the virus variants as the rate of isolation of the pathogenic variant was 3 times as low as that of apathogenic MDV-Kekava, and both virus variants persisted in different cells. When chickens were inoculated simultaneously with both virus variants, the recovery rate of both pathogenic and apathogenic variants from the blood cells was similar. In such cases, persistence of two virus variants in one cell is also possible. The experimental results suggest that in the mechanism of resistance of vaccinated chickens to Marek's disease a significant role may be played by interference between the viruses in which the "occupation" of target cells by vaccine virus protects them from pathogenic MDV.

Animals↗
PubMedDate not supplied

[Study of the synthesis of Rous virus-specific RNA in chick embryo cells].

The presence of virus-specific RNA in commercial chick embryos and its lack in chick embryos of leukemia-free chicken farm of the USSR AMS Oncological Research Center as well as in cell cultures from RIF-free chicken infected with Marek's disease virus was demonstrated by hybridizationof 3H-DNA-product of Rous sarcoma virus synthesized in vitro in the presence of actinomycin D with the total preparation of cellular RNA.

Animals↗
PubMedDate not supplied

[Study of the vaccinating properties of 2 variants of Marek's disease virus].

The vaccinating properties of two variants of Marek's disease virus, Kekava strain (MDV-Kekava) have been studied. One of them is naturally attenuated, the other has been produced as a result of attenuation of the pathogenic MDV-Kekava by long-term passages in chick embryo fibroblast (CEF) culture. The vaccinating properties of the naturally attenuated strains (MDV-Kekava 83) were first studied in an experiment in which the blood of chicken No. 1683 was used as the vaccine. In paralled. the MDV-Kekava 83 variant was isolated from the blood of this chicken in CEF culture. Vaccination of chickens with the blood of this chicken as well as with 1000-3000 PFU MDV-Kekava 83 which had undergone 16, 24, 45, and 46 passages in vitro produced in the chickens resistance to Marek's disease. According to the combined data of 4 experiments, altogether 9 out of 101 vaccinated chickens developed Marek's disease. Among the controls inoculated with the pathogenic MDV alone 51 out of 137 chickens developed the disease. Attenuation of the pathogenic MDV-Kekava 55 in CEF culture occurred very slowly. The virus of the 45th passage in vitro had no vaccinating properties. Vaccination of the chickens with 1000-2500 PFU MDV-Kekava 56 which had undergone 73 a94 passages in CEF culture produced resistance to Marek's disease. Among the vaccinated chickens 20 out of 113 had Marek's disease whereas in the control group 74 out of 122 were observed to develop the disease. Among the chickens vaccinated with MDV, 3-4% developed the disease. Possible causes of the disease in this group of chickens are discussed.

Animals↗
PubMedDate not supplied

[A mixed infection of lymphoid cell lines by the human T-lymphotropic retrovirus type I (HTLV-I) and the measles vaccine virus].

Interaction of HTLV-1 and vaccine strain of measles virus (VM-L16) in different T and B cell lines was studied. VM-L16 replicated in T and B cells with a cytopathic effect. At a multiplicity of infection of 1 TCD50 per 10 cells, all the cells in cultures died within 4-13 days. No effect of HTLV-1 on cell sensitivity to VM-L16 was demonstrated. HTLV-1 produced in various T-cell lines had different syncytium-forming activity in XC cell cultures. Cocultivation of C91/pL cells with XC cells led to the formation of multiple syncytia. HUT-102 cells had no such activity. Infection of these cell lines with VM-L16 did not change their properties. Another HTLV-1-infected cell line, MT-2, caused insignificant aggregation of XC cells. Infection of the latter with VM-L16 increased the number of racemations 8-fold, and they consisted of numerous adhesive cells. The cell adhesion, however, which is the first stage of syncytium formation did nor terminate in cytoplasm confluence. MT-2 nad XC cell lines may be used as model systems for the study of various factors influencing HTLV-1 activation.

B-Lymphocytes↗
PubMedDate not supplied

[Screening of sera from the adult populations of some USSR regions for antibodies to the human T-cell leukemia virus type I (HTLV-I)].

The results of serologic survey of 4118 residents of the USSR Far East from 16 to 82 years of age and 1037 blood donors for antibody to HTLV-I by the method of passive agglutination of gelatin particles coated with viral proteins are summarized. The portion of HTLV-1 seropositive subjects in the Khabarovsk Territory was 1.79% (25 out of 1391), in the Maritime Territory 2.2% (18 out of 801), in the Sakhalin Island 1.6% (26 out of 1557), and in Kamchatka 1.6% (6 out of 369). A discrete area of a higher number of positive cases was found among Nivkhi national group in the village of Nogliki in the Sakhalin Island: 6.4% (10 out of 155). In women, antibody to HTLV-1 was found twice as frequently as in men. Seropositives to HTLV-1 among blood donors in Moscow, the town of Komsomol'sk-na-Amure, and the town of Yuzhno-Sakhalinsk comprised 1.4%, 1.6%, and 5.45%, respectively. The occurrence of HTLV-1 infection among the normal population of the Far East and especially among blood donors attests to the expedience of wider seroepidemiological surveys of the population and especially donors for HTLV-I infection in other regions of the USSR as well.

Adolescent↗
PubMedDate not supplied

[Detection of HTLV-I viral antibodies in donor blood samples].

Sera from 867 donors were tested for antibodies to the virus of T-lymphocyte leukemia of adults (HTLV-I) in parallel by indirect immunofluorescence on acetone-fixed cells of HUT-102 culture producing HTLV-I and by agglutination test using a commercial set ("Serodia-ATLA", Japan). Twelve (1.38%) out of 867 sera were positive in the agglutination test and only 7 of them in immunofluorescence test. The specificity of the results was verified by absorption experiments. The activity of the sera in the agglutination test disappeared completely after their absorption with HUT-102 culture cells but did not change after absorption with LLU cells producing no HTLV-I despite a low percent of virus infection, donor blood control for HTLV-I infection becomes mandatory.

Adult↗
PubMedDate not supplied

[Possible connection of the vaccinia virus and carcinogenesis].

The data are presented indicating a possible carcinogenic danger of vaccinia virus. Vaccinia virus with reduced lytic properties was shown to be capable of inducing morphological and malignant transformation of cells of primary mammal tissue cultures (mouse, rat, man).

Animals↗
PubMedDate not supplied

[Factual nutrition and several indicators of health status of urban schoolchildren of the regions of Ukraine].

Altogether 2334 schoolchildren (first-, fifth- and eighth-graders) were examined for the factual nutrition, health status and physical development. The diet received by the schoolgirls of the above grades and that of the first-grade boys correlated well with the recommended physiological standards, whereas the diet of the eight-grade boys and five-grade boys in particular was slightly lower as compared to those standards. Attention is drawn to an ever increasing number of obese children, which has an adverse effect on the percentage of harmoniously developed children, reducing it to 66.7%. To improve the nutrition of schoolchildren, the authors have developed sets of foods and exemplary menus which have been included into the methodological instructions for organization of schoolchildren's catering in nonspecialized schools of the Ukrainian Soviet Socialist Republic.

Adolescent↗
PubMedDate not supplied

[Use of computers in studying the actual nutritional and health status of children].

The authors have devised a pack of applied programs for statistic data treatment, which can be used for studying problems of nutrition and the health status of children, for design of rational diets by the research institutes and childhood establishments sponsored by the public health and education systems, as well as by trade unions.

Child↗