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Biomedical subjects

L Rosen

Publications and source records attributed to L Rosen.

At least 217 records · Page 12Linked to original sources
PubMed1966

Identification of enteroviruses by hemagglutination-inhibition.

Kern, Jerome (Pacific Research Section, Honolulu, Hawaii), and Leon Rosen. Identification of enteroviruses by hemagglutination-inhibition. J. Bacteriol. 91:1936-1942. 1966.-Approximately 40% of a group of 906 enterovirus isolates cytopathic for monkey cell cultures were found to possess hemagglutinins for human erythrocytes when tested at temperatures of 4 and 37 C and at pH 5.8 and 7.3. The hemagglutinating isolates could be classified by relatively simple techniques into 18 serotypes. Four of these serotypes, echovirus type 24 and coxsackievirus B types 1, 5, and 6, had not previously been known to include hemagglutinating strains. One serotype, Toluca-3, represented a previously unrecognized enterovirus, and two other serotypes may also represent previously unrecognized enteroviruses.

Enterovirus↗
PubMedDate not supplied

Small-bowel obstruction secondary to pericecal hernia.

Pericecal hernia is a rare type of internal hernia and, though congenital in origin, may occur at any age, usually with symptoms of acute intestinal obstruction. The pathophysiology and character of these defects are described, and the management of this situation in an 80-year-old patient is reported. Prompt operative intervention to avoid the associated high mortality secondary to strangulation is urged. It is suggested that closure of these defects be performed if incidentally found at laparotomy.

Aged↗
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A non-invasive means to effectively restore normothermia in cold stressed individuals: a preliminary report.

The combined application of subatmospheric pressure and heat to a forearm and hand has been previously reported to be an effective method for restoring normothermia in hypothermic postoperative patients. The objective of this study was to determine whether this technique also could be useful for the treatment of accidental hypothermia. Four otherwise healthy cold-stressed soldiers (core temperature <36.0 degrees C) were studied. In all four cases, application of the subatmospheric pressure induced an immediate local distention of the subcutaneous vasculature of the hand and fingers. Tympanic temperature reached a plateau in a normothermic range within 15 min in all subjects. The subjects also noted rapid elimination of shivering and improvement in subjective assessment of thermal comfort. These results are encouraging and warrant further investigation of the technique.

Adult↗
PubMedDate not supplied

Preparation of high-titred antisera for sigma virus strains from Hawaii and France and their use in neutralization tests in vitro and in vivo.

Antisera were prepared in rabbits against two strains of sigma virus, from France and Hawaii respectively, using purified virus from cell cultures. All antisera had the same titre by immunofluorescence, 1/400 or 1/800, whether tested against the homologous or the heterologous virus. An antiserum prepared with the French strain neutralized the Hawaiian virus at a dilution of 1/800 both in vitro and in vivo. The Hawaiian virus was not neutralized to a significant extent by reference mouse ascitic fluids for 26 different vertebrate rhabdoviruses.

Aedes↗
PubMedDate not supplied

Doppler echocardiography: application to the assessment of successful thrombolysis of prosthetic valve thrombosis.

Prosthetic valve thrombosis remains a significant problem despite anticoagulation therapy and advances in valve design. Thrombolytic therapy offers an alternative approach to valve replacement in patients with high surgical risk. In this article we discuss three cases in which Doppler echocardiography was used to confirm the diagnosis of prosthetic mitral valve obstruction and serially monitor the response of valvular hemodynamic measurements to thrombolysis with intravenous streptokinase. These cases illustrate how the Doppler technique, in addition to allowing the noninvasive diagnosis of prosthetic valve obstruction, is presently the ideal tool to follow serially the effect of thrombolytic therapy on prosthetic valve function.

Aged↗
PubMedDate not supplied

Recovery of virus from the liver of children with fatal dengue: reflections on the pathogenesis of the disease and its possible analogy with that of yellow fever.

Dengue viruses types 2 or 3 were recovered from the liver of 5 out of 17 children who were suspected to have died of dengue. Among the 5 children with liver isolates, virus was also recovered from the heart blood, spleen and midbrain of one and from the spleen of another. Attention is drawn to the many similarities in the clinical manifestations of yellow fever and severe dengue, their indistinguishable liver lesions and the close relationship of the viruses themselves. In view of the liver isolates reported here and those described by others, it is suggested that the pathogenesis of the two diseases may be analogous.

Antigens, Viral↗
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Disease exacerbation caused by sequential dengue infections: myth or reality?

Dengue can be caused by any one of four viruses that are antigenically related but not cross-protective. It has been proposed that the disease may be more severe if an infection with one dengue serotype follows infection with another serotype. Both experimental and epidemiologic data have been cited to substantiate this concept, but the present reviewer does not find the data convincing. The issue is of practical importance because of its relevance to the use of dengue vaccines now under development. It is impossible to prove that clinical manifestations of dengue are not exacerbated by sequential infections. Negative findings can always be countered by arguments that exacerbation occurs only with certain serotypes, only with certain strains of those serotypes, or only when infections occur at critical intervals. Interested persons can examine the relevant data and decide whether the concept of disease exacerbation following sequential dengue infections is myth or reality.

Dengue↗
PubMedDate not supplied

Myocardial wall motion evaluation using amplitude frequency spectral analysis.

Frequency spectral analysis of cardiac wall motion as calculated from the recorded video kymogram are demonstrated. Characteristic harmonics following the fundamental frequency of the cardiac rate have been demonstrated to have an alternating sequential energy distribution as measured from the free margin of the left ventricle of humans. The characteristic patterns occur in approximately 80% of the 50 patients evaluated. The correlation of these harmonics with physiologic events is not yet complete. Amplitude frequency spectral analysis offers an objective method for analysis of cardiac kymography not otherwise possible.

Adult↗
PubMedDate not supplied

Contrast and electrolyte dynamics of the intravenous pyelogram. I. Urinary pH and volume changes in the canine I. V. P.

Canine peripheral venous or suprarenal aortic injections of sodium or meglumine iothalamate produced a significant swing towards an alkaline urine only in individual dogs injected with meglumine salts. When mean values were compared for the sodium or meglumine groups as a whole, however, no significant differences could be established for pH change or induced diuresis. With both salts, ATPase inhibition could play a role in urinary electrolyte excretion. Carbonic anhydrase inhibition does not seem to play a significant role in urinary pH changes induced by contrast media.

Acetazolamide↗